Researchers have long studied circular dichroism(CD) for its enormous prospects in life sciences. Many biomolecules have vibration modes in the terahertz region, and terahertz CD spectra are robust to detect biomolecu...Researchers have long studied circular dichroism(CD) for its enormous prospects in life sciences. Many biomolecules have vibration modes in the terahertz region, and terahertz CD spectra are robust to detect biomolecular structures. However,few studies explore the terahertz CD spectra on even natural materials due to technical challenges in both fields. Here, we report a setup of home-built terahertz time-domain spectroscopy to measure the polarization states of terahertz waves.By carefully measuring the transmission Jones matrix, we obtain terahertz CD spectra of α-lactose tablets and D-glucose tablets. Our results show that the terahertz CD spectra are sensitive to vibrational motions in biochemical compounds,which will find wide applications in biosensing and biomedical diagnostics.展开更多
Lactose powder consisting of α-D-lactose monohydrate and anhydrous β-D-lactose was nondestructively and quantitatively evaluated by transmission-type Terahertz time-domain spectroscopy (THz-TDS). An absorption with ...Lactose powder consisting of α-D-lactose monohydrate and anhydrous β-D-lactose was nondestructively and quantitatively evaluated by transmission-type Terahertz time-domain spectroscopy (THz-TDS). An absorption with peak at 39.7 cm-1 (1.19 THz) was assigned to be derived from anhydrous β-D-lactose, in addition to the absorptions due to α-D-lactose monohydrate with peak at 17.1 cm-1 (0.53 THz) and 45.6 cm-1 (1.37 THz). After deconvolution of the spectra using Lorentzian, integrated intensities of the absorptions with peak at 39.7 cm-1 and 45.6 cm-1 were uniquely dependent on the weight composition ratio of the α-and β-lactose powder. As a result, the net molar-ratio of the α-and β-lactose in lactose powder could be precisely evaluated by the integrated intensity ratio. Further, anomer content in lactose powder extracted from lactose solution was evaluated and the refined and unrefined features were shown by the evaluation method.展开更多
基金Project supported by the National Natural Science Foundation of China (Grant No. 61775233)。
文摘Researchers have long studied circular dichroism(CD) for its enormous prospects in life sciences. Many biomolecules have vibration modes in the terahertz region, and terahertz CD spectra are robust to detect biomolecular structures. However,few studies explore the terahertz CD spectra on even natural materials due to technical challenges in both fields. Here, we report a setup of home-built terahertz time-domain spectroscopy to measure the polarization states of terahertz waves.By carefully measuring the transmission Jones matrix, we obtain terahertz CD spectra of α-lactose tablets and D-glucose tablets. Our results show that the terahertz CD spectra are sensitive to vibrational motions in biochemical compounds,which will find wide applications in biosensing and biomedical diagnostics.
文摘Lactose powder consisting of α-D-lactose monohydrate and anhydrous β-D-lactose was nondestructively and quantitatively evaluated by transmission-type Terahertz time-domain spectroscopy (THz-TDS). An absorption with peak at 39.7 cm-1 (1.19 THz) was assigned to be derived from anhydrous β-D-lactose, in addition to the absorptions due to α-D-lactose monohydrate with peak at 17.1 cm-1 (0.53 THz) and 45.6 cm-1 (1.37 THz). After deconvolution of the spectra using Lorentzian, integrated intensities of the absorptions with peak at 39.7 cm-1 and 45.6 cm-1 were uniquely dependent on the weight composition ratio of the α-and β-lactose powder. As a result, the net molar-ratio of the α-and β-lactose in lactose powder could be precisely evaluated by the integrated intensity ratio. Further, anomer content in lactose powder extracted from lactose solution was evaluated and the refined and unrefined features were shown by the evaluation method.
