β-elemene通过调节耐药特异性miRNA逆转乳腺癌细胞耐药性的机制研究

目的:探讨β-榄香烯逆转乳腺癌细胞耐药性的机制,明确其抗肿瘤作用。方法:通过向MCF-7细胞株培养基内逐渐增加临床上最常用于乳腺癌化学治疗的药物多西紫杉醇(Doc)或阿霉素(Adr),逐渐提高培养基中药物浓度的方法成功地构建出亲本细胞株MCF-7中耐药亚型,选择乳腺癌阿霉素耐药细胞(MCF-7/Adr)和乳腺癌多西紫杉醇(MCF-7/Doc)耐药细胞株,采用MTT-cytotoxic、miRNA微阵列、实时定量PCR、双荧光素酶活性测定等方法,研究β-elemene对乳腺癌耐药细胞生存率、耐药特异性miRNA29a、miRNA452、miRNA34a及miRNA222表达的影响。结果:β-elemene明显降低耐药乳腺癌细胞株细胞生存率和增殖率,β-elemene显著调节MCF-7/Adr和MCF-7/Doc细胞内miRNAs的表达水平,其中6个miRNAs上调,12个miRNAs下调显著。结论:β-elemene是一种从植物中提取的天然抗肿瘤中药成分,可以逆转乳腺癌细胞的耐药性。证实了中药β-elemene可以通过调控耐药相关miRNA表达的机制抗乳腺癌耐药细胞的耐药性。

β-elemene联合顺铂抑制Hep-2细胞生长作用的实验研究

目的探讨β-elemene联合顺铂对体外培养的Hep-2细胞(人喉咽癌细胞)生长的抑制作用。方法采用MTT比色法和流式细胞分析技术检测不同组别、不同时间(12、24、48h)对Hep-2细胞的抑制其增殖和诱导其凋亡的作用。结果单纯β-elemene组、单纯顺铂组及β-elemene联合顺铂组,12h时对Hep-2细胞增殖抑制率分别为19.05%、20.12%和22.24%,细胞凋亡率分别为12.21%、17.98%和20.34%;24h时细胞增殖抑制率为40.62%、41.51%和64.65%,细胞凋亡率为25.34%、31.08%和34.62%;48h时细胞增殖抑制率为60.50%、68.23%和71.56%,细胞凋亡率为40.63%、42.96%和63.01%。β-elemene联合顺铂组对Hep-2细胞生长抑制作用和诱导细胞凋亡的作用都明显优于其他各组(P<0.05)。结论β-elemene联合顺铂能够明显抑制Hep-2细胞增殖,诱导Hep-2细胞凋亡,为喉咽癌的化疗提供了一个新的启发。

β-elemene enhances the radiosensitivity of gastric cancer cells by inhibiting Pak1 activation

AIM:To explore the potential of β-elemene as a radiosensitizer for gastric cancer cells and the underlying mechanisms.METHODS:SGC7901,MKN45,MKN28,N87,and AGS human gastric cancer cell lines were used to screen for radioresistant gastric cancer cell lines. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium(MTT) assay was used to determine the effects of β-elemene and IPA-3 on cell viability in MKN45 and SGC7901 gastric cancer cell lines. A clonogenic survival assay and annexin V-FITC/PI apoptosis detection assay were used to evaluate cellular radiosensitivity and radiation-induced cell death,respectively. A proteomic method,isobaric tags for relative and absolute quantitation(i TRAQ),was employed to screen the proteins regulated by β-elemene pretreatment prior to ionizing radiation(IR) in SGC7901 gastric cancer cell line. IPA-3 was used as a specific small molecule inhibitor of p21-activated protein kinase 1(Pak1) to target Pak1 signaling. Protein levels of PAK1IP1(p21-activated protein kinase-interacting protein 1),total Pak1(t-Pak1),phospho-Pak1(T423),phospho-ERK1/2( Thr202/Tyr204),and cleaved caspase-3(17 k Da) were assessed by western blotting.RESULTS:MKN45 and SGC7901 gastric cancer cell lines were relatively more resistant to IR. β-elemene pretreatment decreased clonogenic survival following IR in MKN45 and SGC7901 gastric cancer cell lines. Additionally,β-elemene pretreatment prior to IR increased radiation-induced cell death compared with IR alone in MKN45(10.4% ± 0.9% vs 34.8% ± 2.8%,P < 0.05) and SGC7901(11.6% ± 0.9% vs 46.7% ± 5.2%,P < 0.05) human gastric cancer cell lines,respectively,consistent with the level of cleaved caspase-3(17 k Da). Through i TRAQ analysis and western blot validation,we found that β-elemene upregulated PAK1IP1 and downregulated phospho-Pak1(T423) and phosphoERK1/2 in SGC7901 gastric cancer cells. IR increased the level of phospho-Pak1(T423). Pretreatment with β-elemene decreased radiation-induced Pak1 and ERK1/2 phosphorylation. Inhibition of Pak1 using IPA-3 decreased clonogenic survival following IR. In addition,IPA-3 increased radiation-induced cell death in MKN45(13.4% ± 0.3% vs 26.6% ± 1.0%,P < 0.05) and SGC7901(16.0% ± 0.6% vs 37.3% ± 1.7%,P < 0.05) gastric cancer cell lines,respectively,consistent with the level of cleaved caspase-3(17 k Da). Western blotting showed that IPA-3 decreased radiation-induced Pak1 and ERK1/2 phosphorylation.CONCLUSION:This is the first demonstration that β-elemene enhances radiosensitivity of gastric cancer cells,and that the mechanism involves inhibition of Pak1 signaling.

Preparation, characterization, pharmacokinetics and anticancer effects of PEGylated β-elemene liposomes

Objective:This study aimed to develop a new polyethylene glycol(PEG)ylatedβ-elemene liposome(PEG-Lipo-β-E)and evaluate its characterization,pharmacokinetics,antitumor effects and safety in vitro and in vivo.Methods:The liposomes were prepared by ethanol injection and high-pressure micro-jet homogenization.Characterization of the liposomes was conducted,and drug content,entrapment efficiency(EE),in vitro release and stability were studied by ultra-fast liquid chromatography(UFLC)and a liquid surface method.Blood was drawn from rats to establish the pharmacokinetic parameters.The anticancer effect was evaluated in a KU-19-19 bladder cancer xenograft model.Histological analyses were performed to evaluate safety.Results:The PEG-Lipo-β-E showed good stability and was characterized as 83.31±0.181 nm in size,0.279±0.004 in polydispersity index(PDI),-21.4±1.06 mV in zeta potential,6.65±0.02 in pH,5.024±0.107 mg/mL inβ-elemene(β-E)content,and 95.53±1.712%in average EE.The Fourier transform infrared spectroscopy(FTIR)and differential scanning calorimetry(DSC)indicated the formation of PEG-Lipo-β-E.Compared to elemene injection,PEG-Lipo-β-E demonstrated a 1.75-fold decrease in clearance,a 1.62-fold increase in half-life,and a 1.76-fold increase in area under the concentration-time curves(AUCs)from 0 hour to 1.5 hours(P<0.05).PEG-Lipo-β-E also showed an enhanced anticancer effect in vivo.Histological analyses showed that there was no evidence of toxicity to the heart,kidney,liver,lung or spleen.Conclusions:The present study demonstrates PEG-Lipo-β-E as a new formulation with ease of preparation,high EE,good stability,improved bioavailability and antitumor effects.

An ATF24 peptide-functionalized β-elemene-nanostructured lipid carrier combined with cisplatin for bladder cancer treatment

Objective:In this study,we aimed to develop an amino-terminal fragment(ATF)peptide-targeted liposome carryingβ-elemene(ATF24-PEG-Lipo-β-E)for targeted delivery into urokinase plasminogen activator receptor-overexpressing bladder cancer cells combined with cisplatin(DDP)for bladder cancer treatment.Methods:The liposomes were prepared by ethanol injection and high-pressure microjet homogenization.The liposomes were characterized,and the drug content,entrapment efficiency,andin vitro release were studied.The targeting efficiency was investigated using confocal microscopy,ultra-fast liquid chromatography,and an orthotopic bladder cancer model.The effects of ATF24-PEG-Lipo-β-E combined with DDP on cell viability and proliferation were evaluated by a Cell Counting Kit-8(CCK-8)assay,a colony formation assay,and cell apoptosis and cell cycle analyses.The anticancer effects were evaluated in a KU-19-19 bladder cancer xenograft model.Results:ATF24-PEG-Lipo-β-E had small and uniform sizes(~79 nm),high drug loading capacity(~5.24 mg/mL),high entrapment efficiency(98.37±0.95%),and exhibited sustained drug release behavior.ATF24-PEG-Lipo-β-E had better targeting efficiency and higher cytotoxicity than polyethylene glycol(PEG)ylatedβ-elemene liposomes(PEG-Lipo-β-E).DDP,combined with ATF24-PEG-Lipo-β-E,exerted a synergistic effect on cellular apoptosis and cell arrest at the G2/M phase,and these effects were dependent on the caspase-dependent pathway and Cdc25C/Cdc2/cyclin B1 pathways.Furthermore,thein vivo antitumor activity showed that the targeted liposomes effectively inhibited the growth of tumors,using the combined strategy.Conclusions:The present study provided an effective strategy for the targeted delivery ofβ-elemene(β-E)to bladder cancer,and a combined strategy for bladder cancer treatment.

Inhibition of β-elemene on the expressions of HIF-lα, VEGF and i NOS in diabetic rats model

AIM: To evaluate the effect of β-elemene on the expressions of hypoxia-inducible factor(HIF)-lα, vascular endothelial growth factor(VEGF) and inducible nitric oxide synthase(i NOS) in a streptozotocin(STZ) induced diabetic SpragueDawley(SD) rat model.METHODS: SD rats were administered an abdominal injection of STZ and induced to a diabetic model. After 6 wk course of diabetes, the treatment groups were given β-elemene through periocular and intravitreous injection separately and the control groups were given blank emulsion injection. HE staining was used to observe the morphology of retina. The m RNA expressions of HIF-1α, VEGF and i NOS was assayed by real-time polymerase chain reaction(PCR) and the protein expression was measured by Western blot and immunocytochemistry methods.RESULTS: The results indicated that the protein and m RNA expressions of HIF-1α, VEGF and i NOS after treated by β-elemene periocularly and intravitreally injections were all found to be reduced compared with the levels in the diabetic rats group(P<0.05). The inhibitory effect of intravitreal injection was more remarkable.CONCLUSION: The results show β-elemene protect the retina of diabetic rats from high glucose damage by downregulating the expression of HIF-1α, VEGF and iNOS.

ANGⅡ-AT_1 Receptor Pathway Is Involved in the Anti-fibrotic Effect of β-elemene

To investigate the effects of β-elemene on the ANG Ⅱ-ATI receptor pathway in rats with liver fibrosis, a model of hepatic fibrosis was induced by hypodermical injection of carbon tetrachloride （CC14） into Wistar male rats. D-elemene was intraperitonealy administered into the rats for 8 weeks （0.1 mL/100 g body weight per day）. Masson staining was used to observe the liver fibrosis of rats and liver functions were measured by enzymatic kinetic analysis. The content of hydroxyproline in liver tissues was detected by specimen alkaline hydrolysis. The level of plasma ANG Ⅱ in blood plasma was detected by radioimmunoassay. The expression of AT1R in rat liver were measured using reverse transcriptional-polymerase chain reaction and immunohistochemistry respectively. The results showed that β-elemene could reduce the collagen disposition in liver and inhibit the progression of liver fibrosis. In addition, the levels of plasma ANG Ⅱ and the expression of hepatic AT1R in rats with liver fibrosis were also suppressed by β-elemene. It is concluded that the ANG Ⅱ-AT1 receptor pathway plays an important role in the development of hepatic fibrosis and D-elemene could down-regulate the levels of plasma ANG Ⅱ and the expression of hepatic ATIR in rats with liver fibrosis.

β-elemene down-regulates HIF-lα, VEGF and iNOS in human retinal pigment epithelial cells under high glucose conditions

AIM:To investigate the effects and mechanism ofβ-elemene on the expressions of hypoxia-inducible factor-1α(HIF-lα),vascular endothelial growth factor(VEGF)and inducible nitric oxide synthase(iNOS)in human retinal pigment epithelial(RPE)cells under high glucose conditions.METHODS:ARPE-19 cell line was cultured under eight conditions:1)low glucose(LG;5.5 mmol/L);2)high glucose(HG;33 mmol/L);3)high glucose with 20μg/m Lβ-elemene(HG+20 E);4)high glucose with 40μg/m Lβ-elemene(HG+40 E);5)high glucose with SB203590[HG+SB203590,p38-mitogen-activated protein kinase(p38-MAPK)pathway inhibitor];6)high glucose with LY294002[HG+LY294002,phosphoinositide 3-kinase/protein kinase B(PI3 K/Akt)pathway inhibitor];7)high glucose with 40μg/m Lβ-elemene and SB203590(HG+40 E+SB203590);and 8)high glucose with 40μg/m Lβ-elemene and LY294002(HG+40 E+LY294002).Cells were treated in conditions 1-4 for 24 and 48 h,while for 48 h in conditions 5-8.Then m RNA and protein levels of HIF-1α,VEGF and iNOS in cells were measured by real-time polymerase chain reaction(q PCR),immunofluorescence and Western blotting,respectively.Furthermore,protein levels of total p38-MAPK,phosphorylated p38-MAPK(p38-MAPK-P),total Akt and phosphorylated Akt(Akt-P)in cells of conditions 2 and 4 which treated for 48 h were measured by Western blotting.RESULTS:The m RNA levels and protein levels of HIF-1α,VEGF and iNOS in cells were significantly reduced in conditions 3-8 when compared with those in condition 2(P<0.05).These reductions were more obvious in conditions treated for 48 h than in conditions treated for 24 h.The protein levels of p38-MAPK-P and Akt-P in cells of condition 4 were significantly lower than in condition 2(P<0.01).CONCLUSION:β-elemene down-regulates HIF-1α,VEGF and iNOS in ARPE-19 cells under a high glucose condition.The inhibitory effect ofβ-elemene is more significant when its concentration and treatment time are increased,as well as it is combined with SB203590 or LY294002 treatment.P38-MAPK and PI3 K/Akt signaling pathways may play a role in this inhibitory effect.

Radiosensitivity of β-elemene on rabbit VX2 renal transplant carcinoma model

Objective To investigate the effects of low dosage of β-elemene on the radiosensitivity of rabbit VX2 renal transplant carcinoma model. Methods We took the rabbit VX2 renal transplant carcinoma as the model. Experimental rabbits were divided into three groups: the control group, the radiation group, and the radiation +β-elemene (radiosensitivity) group. The change of tumor was observed by Spiral CT and B ultrasound to compare its regrowth period. The tumor was measured by light microscopy and electron microscopy. Results The tumor in radiosensitivity group was restrained obviously and the sensitization enhancement ratio (SER) of β-elemene was 1.89. Different apoptosis was observed under transmission electron microscopy. Conclusion Low dosage β-elemene can enhance the radiosensitivity of rabbit VX2 renal transplant carcinoma model and induce the apoptosis of tumor cells, but the mechanism needs further study. It promotes apoptosis in mechanisms in vitro.

Discovery and bioassay of disubstituted β-elemene-NO donor conjugates:synergistic enhancement in the treatment of leukemia

Natural products are essential sources of antitumor drugs.One such molecule,β-elemene,is a potent antitumor compound extracted from Curcuma wenyujin.In the present investigation,a series of novel 13,14-disubstituted nitric oxide(NO)-donorβelemene derivatives were designed,withβ-elemene as the foundational compound,and subsequently synthesized to evaluate their therapeutic potential against leukemia.Notably,the derivative labeled as compound 13d demonstrated a potent anti-proliferative activity against the K562 cell line,with a high NO release.In vivo studies indicated that compound 13d could effectively inhibit tumor growth,exhibiting no discernible toxic manifestations.Specifically,a significant tumor growth inhibition rate of 62.9%was observed in the K562 xenograft tumor mouse model.The accumulated data propound the potential therapeutic application of compound 13d in the management of leukemia.

β-Elemene induces apoptosis and autophagy in colorectal cancer cells through regulating the ROS/AMPK/mTOR pathway

β-Elemene is an effective anti-cancer ingredient extracted from the genus Curcuma(Zingiberaceae familiy).In the present study,we demonstrated thatβ-elemene inhibited the proliferation of colorectal cancer cells and induced cell cycle arrest in the G2/M phase.In addition,β-elemene induced nuclear chromatin condensation and cell membrane phosphatidylserine eversion,decreased cell mitochondrial membrane potential,and promoted the cleavage of caspase-3,caspase-9 and PARP proteins,indicating apoptosis in colorectal cancer cells.At the same time,β-elemene induced autophagy response,and the treated cells showed autophagic vesicle bilayer membrane structure,which was accompanied by up-regulation of the expression of LC3B and SQSTM1.Furthermore,β-elemene increased ROS levels in colorectal cancer cells,promoted phosphorylation of AMPK protein,and inhibited mTOR protein phosphorylation.In the experiments in vivo,β-elemene inhibited the tumor size and induced apoptosis and autophagy in nude mice.In summary,β-elemene inhibited the occurrence and development of colon cancer xenografts in nude mice,and significantly induced apoptosis and autophagy in colorectal cancer cells in vitro.These effects were associated with regulation of the ROS/AMPK/mTOR signaling.We offered a molecular basis for the development ofβ-elemene as a promising anti-tumor drug candidate for colorectal cancer.

ETME,a novel β-elemene derivative,synergizes with arsenic trioxide in inducing apoptosis and cell cycle arrest in hepatocarcinoma cells via a p53-dependent pathway

Arsenic trioxide(ATO)has been identified as an effective treatment for acute promyelocytic leukemia(APL)but is much less effective against solid tumors such as hepatocellular carcinoma(HCC).In the search for ways to enhance its therapeutic efficacy against solid tumors,we have examined its use in combination with a novel derivative ofβ-elemene,N-(β-elemene-13-yl)tryptophan methyl ester(ETME).Here we report the effects of the combination on cell viability,apoptosis,the cell cycle and mitochondria membrane potential(MMP)in HCC SMMC-7721 cells.We found that the two compounds acted synergistically to enhance antiproliferative activity and apoptosis.The combination also decreased the MMP,down-regulated Bcl-2 and pro-proteins of the caspase family,and up-regulated Bax and BID,all of which were reversed by the p53 inhibitor,pifithrin-α.In addition,the combination induced cell cycle arrest at the G2/M phase and reduced tumor volume and weight in an xenograft model of nude mice.Overall,the results suggest that ETME in combination with ATO may be useful in the treatment of HCC patients particularly those unresponsive to ATO alone.

Synthesis of 13-β-elemene ester derivatives and evaluation of their antioxidant activity in human umbilical vein endothelial cells

In the present study, a series of 13-β-elemene ester derivatives were designed and prepared, and their antioxidant activity was investigated in the H2O2-treated human umbilical vein endothelial cells(HUVECs). Among the test compounds, the dimer compounds 5v and 5w exhibited the most potent antioxidant activity with significant ROS suppression being observed. Both compounds markedly inhibited the H2O2-induced changes in various biochemical substances, such as superoxide dismutase(SOD), malonyldialdehyde(MDA), nitric oxide(NO), and lactic dehydrogenase(LDH), which were superior to that of the positive control vitamin E. Furthermore, they did not produce any obvious cytotoxicity, but increased the viability of HUVECs injured by H2O2 in a dose-dependent manner. Additionally, compound 5w, designed as a prodrug-like compound, showed improved stability relative to compound 4 in vitro.

β-Elemene-loaded polymeric micelles intensify anti-carcinoma efficacy and alleviate side effects

β-Elemene is a volatile oil used for the treatment of cancer,but poor solubility,low bioavailability,and various adverse reactions limit its application.For amelio rating risks of the venous toxicity ofβ-elemene,intravenously injectable micelle ofβ-elemene was prepared using the thin-film hydration method.The results pointed out the micelles were uniformly spherical with about 20.96±0.1966 nm in average diameter and exhibited high entrapment efficiency(99.02%±0.88%).As revealed by drug release studies in vitro,β-elemene micelles had sustained drug release.Compared with freeβ-elemene,the micelles increased the drug cellular uptake and enhanced the anti-tumor effect in vitro through retarding cell cycle and inducing apoptosis.Meanwhile,the elevated se rum stability o fβ-elemene micelles implied less drug leakage and reduced toxicity.The wound healing and tube formation assay in vitro demonstrated the anti-metastasis and anti-angiogenesis effects ofβ-elemene micelles.Moreover,the pharmacokinetics study showed the AUC and T1/2 ofβ-elemene in micelle group were 1.79 and 1.62 times of that in free fi-elemene group,suggesting the circulation time ofβ-elemene in the blood had been prolonged.In addition,β-elemene micelles showed a favorable antitumor response compared with theβ-elemene solution on C26 colon cance r-bearing mice model.Local irritation study investigated in rabbits indicated that theβ-elemene micelles strikingly mitigated the irritation to the injection sites compared with freeβ-elemene.These results proved that the micelle could be a good candidate as an auspicious drug delivery system ofβ-elemene for the prospective clinical treatment of carcinoma.

Immunotherapeutic effects on murine pancreatic carcinoma by β-elemene combined with dendritic cells modified with genes encoding interleukin-23

The dendritic cell vaccine is a treatment vaccine with potent clinical applications.Functional cytokines can enhance dendritic cell anti-tumor immune responses.This experiment was conducted to study the effects of bone marrow-derived dendritic cells(BM-DCs)modified with genes encoding murine interleukin-23(IL-23)on murine pancreatic carcinoma,and effects of the treatment of pancreatic carcinoma with β-elemene combined with IL-23-modified dendritic cell vaccine.The murine IL-23 cDNA was sub-cloned into a dual-expression vector.DCs were pulsed with tumor cell lysate after being modified wth IL-23.Mice were divided into groups which were injected with IL-23-transduced DC vaccine,non-transduced DC vaccine and sodium respectively.The preventive immune and immunotherapeutic effects of DC vaccines on mice and cytokine release in vivo were then assessed.Results showed inhibitory effects on tumor cells and increased survival time in the experimental group treated with the vaccine combined with β-elemene.The IL-23 protein apparently increases the antigen presenting ability of DCs.After injection with DC vaccines,IFN-γ production in the treatment group was significantly increased as compared with that in the control group(P<0.01),and IL-4 production was decreased as compared with that in the control group(P<0.05).Tumor size was obviously reduced,and survival time clearly prolonged in the group with β-elemene combined with DC vaccine,in comparison to the other treatment groups and the control(P<0.01).IL-23-modified dendritic cell vaccines enhance specific Th1-type and cytotoxic T lymphocyte(CTL)responses against pancreatic carcinoma cells,and induce not only auto-immune ability but also preventive immunity against pancreatic carcinoma implanted in mice.β-elemene has great anti-tumor collaborative functions.

Longest survival with primary intracranial malignant melanoma:A case report and literature review

BACKGROUND Primary intracranial malignant melanoma(PIMM)is rare,and its prognosis is very poor.It is not clear what systematic treatment strategy can achieve long-term survival.This case study attempted to identify the optimal strategy for long-term survival outcomes by reviewing the PIMM patient with the longest survival following comprehensive treatment and by reviewing the related literature.CASE SUMMARY The patient is a 47-year-old Chinese man who suffered from dizziness and gait disturbance.He underwent surgery for right cerebellum melanoma and was subsequently diagnosed by pathology in June 2000.After the surgery,the patient received three cycles of chemotherapy but relapsed locally within 4 mo.Following the second surgery for total tumor resection,the patient received an injection of Newcastle disease virus-modified tumor vaccine,interferon,andβ-elemene treatment.The patient was tumor-free with a normal life for 21 years before the onset of the recurrence of melanoma without any symptoms in July 2021.A third gross-total resection with adjuvant radiotherapy and temozolomide therapy was performed.Brain magnetic resonance imaging showed no residual tumor or recurrence 3 mo after the 3rd operation,and the patient recovered well without neurological dysfunction until the last follow-up in June 2022,which was 22 years following the initial treatment.CONCLUSION It is important for patients with PIMM to receive comprehensive treatment to enable the application of the most appropriate treatment strategies.Long-term survival is not impossible in patients with these malignancies.

Chemical Constituents and Antimicrobial Activity of Essential Oil of <i>Senna podocarpa</i>(Guill. et Perr.) Lock

The chemistry of essential obtained from the leaves of Senna podocarpa (Guill. Et Perr.) Lock from Nigeria is being reported. The hydrodistilled oil was analyzed by gas chromatography-flame ionization detector (GC-FID) and gas chromatography-mass spectrometry (GC-MS) techniques. The main constituents of the oil were 1,2-benzenedicarboxylic acid, mono (2-ethylhexyl) ester phthalate (26.6%) and β-elemene (27.9%). There were significant amounts of caryophyllene oxide (7.3%) and urs-12-en-24-oic acid, 3-oxo-methyl ester (5.5%). The antimicrobial activity of the essential oil was evaluated against a panel of seven bacteria and two fungal strain using agar diffusion and broth microdilution methods. Results had shown that the oil exhibited moderate to strong antimicrobial activity against the tested microorganisms. The activity zones of inhibition (ZI) ranged between 10.0 ± 0.2 mm and 28.3 ± 2.9 mm while the minimum inhibitory concentrations (MIC) ranged between 0.3 mg/mL 5.0 mg/mL, respectively. The chemical constituents and antimicrobial activity of the essential oil of Senna podocarpa were being reported for the first time.