The freeze-extraction and freeze-gelation methods were used to prepare highly porous β-TCP/CS scaffolds with different β-TCP/CS ratio.In these methods,the suspending mixture of β-TCP and chitosan was frozen,the fro...The freeze-extraction and freeze-gelation methods were used to prepare highly porous β-TCP/CS scaffolds with different β-TCP/CS ratio.In these methods,the suspending mixture of β-TCP and chitosan was frozen,the frozen mixture was immersed in a non-solvent（0.05mol/L NaOH/ehanol aqueous） bath to allow the exchange between solvent（acetic-acid aqueous） and non-solvent at a temperature lower than the freezing point of the acetic-acid.Then,the β-TCP/CS scaffolds were formed and dried at room temperature.Scanning electron microscopy （SEM）,X-ray diffraction （XRD）,gas chromatography （GC） and omnipotence material testing machine were employed to characterize the β-TCP/CS scaffolds.The results of GC show that the freeze extraction of the β-TCP/chitosan scaffolds was completed when the extraction time is above 24 h.The SEM results show that the β-TCP/CS scaffolds are composed of interconnected pore network.The porosity of the β-TCP/CS scaffolds decrease with the increase of the content of the β-TCP.The β-TCP/CS scaffolds have a highest compressive strength when the chitosan/β-TCP ratio is 30：70.The present work displays that the β-TCP/CS composite scaffolds with appropriate mechanical properties and high porosity can be successfully prepared by the freeze-extraction and freeze-gelation methods.展开更多
基金Funded by the Funds from Science and Technology Commission of Shanghai Municipality(0852nm05200)
文摘The freeze-extraction and freeze-gelation methods were used to prepare highly porous β-TCP/CS scaffolds with different β-TCP/CS ratio.In these methods,the suspending mixture of β-TCP and chitosan was frozen,the frozen mixture was immersed in a non-solvent（0.05mol/L NaOH/ehanol aqueous） bath to allow the exchange between solvent（acetic-acid aqueous） and non-solvent at a temperature lower than the freezing point of the acetic-acid.Then,the β-TCP/CS scaffolds were formed and dried at room temperature.Scanning electron microscopy （SEM）,X-ray diffraction （XRD）,gas chromatography （GC） and omnipotence material testing machine were employed to characterize the β-TCP/CS scaffolds.The results of GC show that the freeze extraction of the β-TCP/chitosan scaffolds was completed when the extraction time is above 24 h.The SEM results show that the β-TCP/CS scaffolds are composed of interconnected pore network.The porosity of the β-TCP/CS scaffolds decrease with the increase of the content of the β-TCP.The β-TCP/CS scaffolds have a highest compressive strength when the chitosan/β-TCP ratio is 30：70.The present work displays that the β-TCP/CS composite scaffolds with appropriate mechanical properties and high porosity can be successfully prepared by the freeze-extraction and freeze-gelation methods.
