In the study,the reasonable sampling of the grey Aneurolepidium chinense of green grassland,the grey-green A.chinense of green grassland,the grey A.chinense of Wulimu and the grey-green A.chinense of Wulimu were analy...In the study,the reasonable sampling of the grey Aneurolepidium chinense of green grassland,the grey-green A.chinense of green grassland,the grey A.chinense of Wulimu and the grey-green A.chinense of Wulimu were analyzed by ISSR.Eight primers with clear and diverse products were screened out from 20 primers and 47 DNA fragments were amplified from 39 individuals.The average number of DNA fragments produced by each primer was 5.9,and polymorphic bands were 41 and the polymorphic rate was 87.23%,which could r...展开更多
Leymus chinensis （Trin.） Tzvel is the characteristic of the Eastern Asia, and is a perennial herbaceous rhizome species on L. chinensis steppe of the Northern China, the Eastern Mongolia, and area of Outer-Baikal of...Leymus chinensis （Trin.） Tzvel is the characteristic of the Eastern Asia, and is a perennial herbaceous rhizome species on L. chinensis steppe of the Northern China, the Eastern Mongolia, and area of Outer-Baikal of Russia. It has both vegetative and sexual reproductions, and results in complexity of the population structure. Thus, it is difficult to identify distinct clones. Author used the randomly amplified polymorphic DNA （RAPD） technique to identify the clonal structure of heterogeneous and homogeneous habitats in two populations ofLeymus chinensis, analyzed 260 ramets samples using 12 primers and 60 polymorphie markers （reproducible RAPD bands）. The results proved elonal diversity described using PD, D, and E was higher in the HCS habitat than that in the HPS habitat. The PD, D, and E in the HCS of Songzhan population were 0.21, 0.90, and 0.91, respectively, whereas the PD, D, and E in the HPS of Songzhan population were only 0.09, 0.68, and 0.77, respectively; the PD, D, and E in the HCS of Yongle population were 0.19, 0.92 mad 0.90, respectively, correspondingly, these measures in the HPS of Yongle population were only 0.09, 0.63, and 0.79, respectively. The results also indicated that clonal population ofL. chinensis in HPS habitat had lower genetic dissimilarity index than that in the HCS habitat.展开更多
研究了从芦苇(Phragmitis communis Trin)中分离得到的化感组分对羊角月牙藻(Selenastrum capricornutum)和雷氏衣藻(Chlamydomonas reinhardtii)生长特性的影响．在藻类生长的对数期向培养液中投加不同浓度的化感组分,分别测定并观察...研究了从芦苇(Phragmitis communis Trin)中分离得到的化感组分对羊角月牙藻(Selenastrum capricornutum)和雷氏衣藻(Chlamydomonas reinhardtii)生长特性的影响．在藻类生长的对数期向培养液中投加不同浓度的化感组分,分别测定并观察了培养期间受试藻种藻密度和藻细胞形态的变化情况．结果表明,该化感组分对羊角月牙藻藻密度的增长具有明显的抑制作用,半效应浓度(EC_(50．7d))值为0．60m/L,同时使羊角月牙藻细胞内部结构改变,形态变大．投加6mg/L化感组分后,藻细胞平均宽度是对照组的1．5倍．该化感组分对雷氏衣藻藻密度的增长没有明显的抑制作用,但使其运动性能降低．展开更多
文摘In the study,the reasonable sampling of the grey Aneurolepidium chinense of green grassland,the grey-green A.chinense of green grassland,the grey A.chinense of Wulimu and the grey-green A.chinense of Wulimu were analyzed by ISSR.Eight primers with clear and diverse products were screened out from 20 primers and 47 DNA fragments were amplified from 39 individuals.The average number of DNA fragments produced by each primer was 5.9,and polymorphic bands were 41 and the polymorphic rate was 87.23%,which could r...
基金Supported by the National Natural Foundation of China (30670336, 30870423, 30870391)
文摘Leymus chinensis （Trin.） Tzvel is the characteristic of the Eastern Asia, and is a perennial herbaceous rhizome species on L. chinensis steppe of the Northern China, the Eastern Mongolia, and area of Outer-Baikal of Russia. It has both vegetative and sexual reproductions, and results in complexity of the population structure. Thus, it is difficult to identify distinct clones. Author used the randomly amplified polymorphic DNA （RAPD） technique to identify the clonal structure of heterogeneous and homogeneous habitats in two populations ofLeymus chinensis, analyzed 260 ramets samples using 12 primers and 60 polymorphie markers （reproducible RAPD bands）. The results proved elonal diversity described using PD, D, and E was higher in the HCS habitat than that in the HPS habitat. The PD, D, and E in the HCS of Songzhan population were 0.21, 0.90, and 0.91, respectively, whereas the PD, D, and E in the HPS of Songzhan population were only 0.09, 0.68, and 0.77, respectively; the PD, D, and E in the HCS of Yongle population were 0.19, 0.92 mad 0.90, respectively, correspondingly, these measures in the HPS of Yongle population were only 0.09, 0.63, and 0.79, respectively. The results also indicated that clonal population ofL. chinensis in HPS habitat had lower genetic dissimilarity index than that in the HCS habitat.
文摘研究了从芦苇(Phragmitis communis Trin)中分离得到的化感组分对羊角月牙藻(Selenastrum capricornutum)和雷氏衣藻(Chlamydomonas reinhardtii)生长特性的影响．在藻类生长的对数期向培养液中投加不同浓度的化感组分,分别测定并观察了培养期间受试藻种藻密度和藻细胞形态的变化情况．结果表明,该化感组分对羊角月牙藻藻密度的增长具有明显的抑制作用,半效应浓度(EC_(50．7d))值为0．60m/L,同时使羊角月牙藻细胞内部结构改变,形态变大．投加6mg/L化感组分后,藻细胞平均宽度是对照组的1．5倍．该化感组分对雷氏衣藻藻密度的增长没有明显的抑制作用,但使其运动性能降低．
文摘为了克隆与研究盐胁迫相关的基因,以盐胁迫后的短芒大麦叶片为材料,用Trizol一步法提取总RNA,Oligo(dT)纤维素柱纯化mRNA,反转录合成第一链cDNA和第二链cDNA.cDNA纯化后,与λZAP表达载体连接,体外包装后得到盐胁迫下短芒大麦cDNA文库,其重组率达96%,滴度为2×107 pfu/mL,插入片段长度0.5～3.0 kb.
