目的 :探索恶性胸腹水上清中游离胸苷酸合成酶(TS)、β-tubulinⅢm RNA的表达,以及游离TS、β-tubulinⅢ表达和患者5-氟尿嘧啶(5-FU)、多西紫杉醇药物敏感性的关系。方法:收集43例进展期肿瘤患者的恶性胸/腹水,分离、提取游离核酸,分离...目的 :探索恶性胸腹水上清中游离胸苷酸合成酶(TS)、β-tubulinⅢm RNA的表达,以及游离TS、β-tubulinⅢ表达和患者5-氟尿嘧啶(5-FU)、多西紫杉醇药物敏感性的关系。方法:收集43例进展期肿瘤患者的恶性胸/腹水,分离、提取游离核酸,分离肿瘤细胞。实时荧光定量RT-PCR分别检测游离上清和肿瘤细胞中TS和β-tubulinⅢm RNA表达;ATP-TCA方法检测肿瘤细胞5-FU、多西紫杉醇的敏感性。结果:TS和β-tubulinⅢm RNA在恶性胸/腹水游离上清中的检出率为98%。与配对的肿瘤细胞相比,游离上清中TS m RNA表达水平高(P=0.02)。在胃肠肿瘤患者中,游离上清中TS m RNA表达水平与5-FU药物敏感性相关(P=0.002)。尽管观察到游离上清中β-tubulinⅢm RNA高表达者多西紫杉醇药物敏感性高的趋势,但差异没有统计学意义。结论:晚期肿瘤患者恶性胸腹水游离上清中可检测到游离TS、β-tubulin III m RNA的表达。在胃肠肿瘤患者中,游离上清TS m RNA表达水平与5-FU药物敏感相关。展开更多
Many studies have shown that fibronectin type III domain-containing protein 5(FDNC5) and brain-derived neurotrophic factor(BDNF) play vital roles in plasticity after brain injury. An enriched environment refers to an ...Many studies have shown that fibronectin type III domain-containing protein 5(FDNC5) and brain-derived neurotrophic factor(BDNF) play vital roles in plasticity after brain injury. An enriched environment refers to an environment that provides animals with multi-sensory stimulation and movement opportunities. An enriched environment has been shown to promote the regeneration of nerve cells, synapses, and blood vessels in the animal brain after cerebral ischemia;however, the exact mechanisms have not been clarified. This study aimed to determine whether an enriched environment could improve neurobehavioral functions after the experimental inducement of cerebral ischemia and whether neurobehavioral outcomes were associated with the expression of FDNC5 and BDNF. This study established ischemic mouse models using permanent middle cerebral artery occlusion(pMCAO) on the left side. On postoperative day 1, the mice were randomly assigned to either enriched environment or standard housing condition groups. Mice in the standard housing condition group were housed and fed under standard conditions. Mice in the enriched environment group were housed in a large cage, containing various toys, and fed with a standard diet. Sham-operated mice received the same procedure, but without artery occlusion, and were housed and fed under standard conditions. On postoperative days 7 and 14, a beam-walking test was used to assess coordination, balance, and spatial learning. On postoperative days 16–20, a Morris water maze test was used to assess spatial learning and memory. On postoperative day 15, the expression levels of FDNC5 and BDNF proteins in the ipsilateral cerebral cortex were analyzed by western blot assay. The results showed that compared with the standard housing condition group, the motor balance and coordination functions(based on beam-walking test scores 7 and 14 days after operation), spatial learning abilities(based on the spatial learning scores from the Morris water maze test 16–19 days after operation), and memory abilities(based on the memory scores of the Morris water maze test 20 days after operation) of the enriched environment group improved significantly. In addition, the expression levels of FDNC5 and BDNF proteins in the ipsilateral cerebral cortex increased in the enriched environment group compared with those in the standard housing condition group. Furthermore, the Pearson correlation coefficient showed that neurobehavioral functions were positively associated with the expression levels of FDNC5 and BDNF(r = 0.587 and r = 0.840, respectively). These findings suggest that an enriched environment upregulates FDNC5 protein expression in the ipsilateral cerebral cortex after cerebral ischemia, which then activates BDNF protein expression, improving neurological function. BDNF protein expression was positively correlated with improved neurological function. The experimental protocols were approved by the Institutional Animal Care and Use Committee of Fudan University, China(approval Nos. 20160858 A232, 20160860 A234) on February 24, 2016.展开更多
Objective:To exploit- the effects of gentiana scabra bage on the expression of hepatic collagen proteins in Paragonimus skrjabini rats with liver fibrosis.Methods:Immunohistochemical technique was used to observe the ...Objective:To exploit- the effects of gentiana scabra bage on the expression of hepatic collagen proteins in Paragonimus skrjabini rats with liver fibrosis.Methods:Immunohistochemical technique was used to observe the changes of content of hepatic type Ⅰ,Ⅲ collagen proteins in Paragonimus skrjabini rats with Liver fibrosis before and after the gentiana scabra bage treatmeat.Results:Comparing with the model group,changes of hepatic tvpe Ⅰ and type Ⅲ collagen proteins in gentiana scabra bage treated group were significantly weakened.Conclusions:Gentiana scabra bage treatment can reduce the content of hepatic type Ⅲ and type Ⅰ collagen protein significantly in Paragonimus skrjabini rats with liver fibrosis,thereby,playing the role against hepatic fibrosis.展开更多
文摘目的 :探索恶性胸腹水上清中游离胸苷酸合成酶(TS)、β-tubulinⅢm RNA的表达,以及游离TS、β-tubulinⅢ表达和患者5-氟尿嘧啶(5-FU)、多西紫杉醇药物敏感性的关系。方法:收集43例进展期肿瘤患者的恶性胸/腹水,分离、提取游离核酸,分离肿瘤细胞。实时荧光定量RT-PCR分别检测游离上清和肿瘤细胞中TS和β-tubulinⅢm RNA表达;ATP-TCA方法检测肿瘤细胞5-FU、多西紫杉醇的敏感性。结果:TS和β-tubulinⅢm RNA在恶性胸/腹水游离上清中的检出率为98%。与配对的肿瘤细胞相比,游离上清中TS m RNA表达水平高(P=0.02)。在胃肠肿瘤患者中,游离上清中TS m RNA表达水平与5-FU药物敏感性相关(P=0.002)。尽管观察到游离上清中β-tubulinⅢm RNA高表达者多西紫杉醇药物敏感性高的趋势,但差异没有统计学意义。结论:晚期肿瘤患者恶性胸腹水游离上清中可检测到游离TS、β-tubulin III m RNA的表达。在胃肠肿瘤患者中,游离上清TS m RNA表达水平与5-FU药物敏感相关。
基金supported by the National Natural Science Foundation of China,Nos.81601961(to KWY),81672242(to YW)the Key Construction Projects of Shanghai Health and Family Planning on Weak Discipline,China,No.2015ZB0401(to YW)
文摘Many studies have shown that fibronectin type III domain-containing protein 5(FDNC5) and brain-derived neurotrophic factor(BDNF) play vital roles in plasticity after brain injury. An enriched environment refers to an environment that provides animals with multi-sensory stimulation and movement opportunities. An enriched environment has been shown to promote the regeneration of nerve cells, synapses, and blood vessels in the animal brain after cerebral ischemia;however, the exact mechanisms have not been clarified. This study aimed to determine whether an enriched environment could improve neurobehavioral functions after the experimental inducement of cerebral ischemia and whether neurobehavioral outcomes were associated with the expression of FDNC5 and BDNF. This study established ischemic mouse models using permanent middle cerebral artery occlusion(pMCAO) on the left side. On postoperative day 1, the mice were randomly assigned to either enriched environment or standard housing condition groups. Mice in the standard housing condition group were housed and fed under standard conditions. Mice in the enriched environment group were housed in a large cage, containing various toys, and fed with a standard diet. Sham-operated mice received the same procedure, but without artery occlusion, and were housed and fed under standard conditions. On postoperative days 7 and 14, a beam-walking test was used to assess coordination, balance, and spatial learning. On postoperative days 16–20, a Morris water maze test was used to assess spatial learning and memory. On postoperative day 15, the expression levels of FDNC5 and BDNF proteins in the ipsilateral cerebral cortex were analyzed by western blot assay. The results showed that compared with the standard housing condition group, the motor balance and coordination functions(based on beam-walking test scores 7 and 14 days after operation), spatial learning abilities(based on the spatial learning scores from the Morris water maze test 16–19 days after operation), and memory abilities(based on the memory scores of the Morris water maze test 20 days after operation) of the enriched environment group improved significantly. In addition, the expression levels of FDNC5 and BDNF proteins in the ipsilateral cerebral cortex increased in the enriched environment group compared with those in the standard housing condition group. Furthermore, the Pearson correlation coefficient showed that neurobehavioral functions were positively associated with the expression levels of FDNC5 and BDNF(r = 0.587 and r = 0.840, respectively). These findings suggest that an enriched environment upregulates FDNC5 protein expression in the ipsilateral cerebral cortex after cerebral ischemia, which then activates BDNF protein expression, improving neurological function. BDNF protein expression was positively correlated with improved neurological function. The experimental protocols were approved by the Institutional Animal Care and Use Committee of Fudan University, China(approval Nos. 20160858 A232, 20160860 A234) on February 24, 2016.
基金supported by National Natural Science Foundation of China(No.81360252:81360128)Natural Science Foundation of Yunnan Province(No.2012FB025)
文摘Objective:To exploit- the effects of gentiana scabra bage on the expression of hepatic collagen proteins in Paragonimus skrjabini rats with liver fibrosis.Methods:Immunohistochemical technique was used to observe the changes of content of hepatic type Ⅰ,Ⅲ collagen proteins in Paragonimus skrjabini rats with Liver fibrosis before and after the gentiana scabra bage treatmeat.Results:Comparing with the model group,changes of hepatic tvpe Ⅰ and type Ⅲ collagen proteins in gentiana scabra bage treated group were significantly weakened.Conclusions:Gentiana scabra bage treatment can reduce the content of hepatic type Ⅲ and type Ⅰ collagen protein significantly in Paragonimus skrjabini rats with liver fibrosis,thereby,playing the role against hepatic fibrosis.
