Long non-coding RNA H19 regulates neurogenesis of induced neural stem cells in a mouse model of closed head injury

Stem cell-based therapies have been proposed as a potential treatment for neural regeneration following closed head injury.We previously reported that induced neural stem cells exert beneficial effects on neural regeneration via cell replacement.However,the neural regeneration efficiency of induced neural stem cells remains limited.In this study,we explored differentially expressed genes and long non-coding RNAs to clarify the mechanism underlying the neurogenesis of induced neural stem cells.We found that H19 was the most downregulated neurogenesis-associated lnc RNA in induced neural stem cells compared with induced pluripotent stem cells.Additionally,we demonstrated that H19 levels in induced neural stem cells were markedly lower than those in induced pluripotent stem cells and were substantially higher than those in induced neural stem cell-derived neurons.We predicted the target genes of H19 and discovered that H19 directly interacts with mi R-325-3p,which directly interacts with Ctbp2 in induced pluripotent stem cells and induced neural stem cells.Silencing H19 or Ctbp2 impaired induced neural stem cell proliferation,and mi R-325-3p suppression restored the effect of H19 inhibition but not the effect of Ctbp2 inhibition.Furthermore,H19 silencing substantially promoted the neural differentiation of induced neural stem cells and did not induce apoptosis of induced neural stem cells.Notably,silencing H19 in induced neural stem cell grafts markedly accelerated the neurological recovery of closed head injury mice.Our results reveal that H19 regulates the neurogenesis of induced neural stem cells.H19 inhibition may promote the neural differentiation of induced neural stem cells,which is closely associated with neurological recovery following closed head injury.

SETD5介导AKT1磷酸化调控结肠癌细胞的迁移和5-FU敏感性

目的:探讨含SET结构域蛋白5(SETD5)对结肠癌细胞增殖、迁移和对5-氟尿嘧啶(5-FU)药物敏感性的影响及机制。方法:常规培养结肠癌细胞,用Lipofectamine 2000将siSETD5-NC、si-SETD5-1~3质粒转染至HT-29细胞中,将其分为对照组(未处理)、si-SETD5-NC组、si-SETD5组和si-SETD5+SC79组,si-SETD5+SC79组HT-29细胞转染质粒的同时用10µmol/L SC79处理。qPCR法检测NCM460、HT-29和LoVo细胞中SETD5 mRNA表达,流式细胞术、细胞划痕法、WB法和CCK-8法分别检测各组HT-29细胞的凋亡情况、迁移能力、相关蛋白的表达,以及对5-FU的敏感性。结果:SETD5 mRNA在HT-29、LoVo细胞中均呈高表达(均P<0.01)。在HT-29细胞中成功地敲减了SETD5 mRNA(P<0.01)。敲减SETD5 mRNA可明显抑制HT-29细胞的增殖活性(P<0.01)、迁移能力(P<0.01)、相关蛋白(SETD5、p-PI3K、p-AKT1、p-mTOR蛋白)的表达(均P<0.01)、促进细胞凋亡(P<0.01),且提高其对5-FU的敏感性(P<0.01),这些作用均可被AKT激活剂SC79部分阻挡(P<0.05或P<0.01)。结论:SETD5在HT-29、LoVo细胞中高表达,SETD5通过PI3K/AKT1通路促进结肠癌HT-29细胞的增殖、迁移,且降低其对5-FU的敏感性,SETD5是结肠癌临床诊断、治疗的潜在靶点。

Intuitionistic Fuzzy α-Generalized Closed Sets in Terms of Minimal Structure Spaces

In this paper, we introduce the notion of intuitionistic fuzzy α-generalized closed sets in intuitionistic fuzzy minimal structure spaces and investigate some of their properties. Further, we introduce and study the concept of intuitionistic fuzzy α-generalized minimal continuous functions.

miR-127-3p靶向调节SETD8对胃癌细胞增殖、迁移和侵袭的影响

目的探讨微小RNA(miR)-127-3p对胃癌细胞增殖、迁移及侵袭的影响及其作用机制。方法采用qRT-聚合酶链反应(PCR)检测150例胃癌患者胃癌组织、癌旁组织及人胃上皮细胞GES1、人胃癌细胞SGC7901、AGS、MKN-45、MGC-803中miR-127-3p表达水平。双荧光素酶报告基因检测miR-127-3p与含SET结构域赖氨酸甲基转移酶(SETD)8的靶向调控关系。将SGC7901细胞分为对照(Control)组、miR-127-3p模拟物(miR-127-3p mimics)组及miR-NC组、沉默SETD8表达(si-SETD8)组、si-NC组、miR-127-3p mimics+SETD8过表达(miR-127-3p mimics+SETD8)组及空载质粒(miR-127-3p mimics+pcDNA)组。噻唑蓝(MTT)法、划痕愈合实验、Transwell小室实验分别检测SGC7901细胞增殖、迁移与侵袭;Western印迹检测SGC7901细胞SETD8、基质金属蛋白酶(MMP)-2、MMP-9蛋白表达;裸鼠成瘤实验检测过表达miR-127-3p对裸鼠胃癌移植瘤生长的影响。结果miR-127-3p在胃癌组织与细胞中较癌旁组织和正常细胞显著下调(P<0.05),SETD8 mRNA在胃癌组织中较癌旁组织显著上调(P<0.05);胃癌组织中miR-127-3p、SETD8 mRNA表达水平呈负相关(r=-0.553,P<0.05);双荧光素酶实验证实miR-127-3p与SETD8存在靶向调控关系(P<0.05);过表达miR-127-3p或抑制SETD8表达显著抑制SGC7901细胞增殖、迁移与侵袭(P<0.05);过表达SETD8可部分逆转过表达miR-127-3p对SGC7901细胞增殖、迁移与侵袭的抑制作用(P<0.05);裸鼠成瘤实验表明过表达miR-127-3p可显著抑制胃癌移植瘤生长(P<0.05)。结论miR-127-3p在胃癌组织与细胞中表达下调,过表达miR-127-3p可靶向抑制SETD8表达而抑制胃癌肿瘤生长。

Finite-time stability with respect to a closed invariant set for a class of discontinuous systems

This paper discusses the problem of finite-time stability with respect to a closed, but not necessarily compact, invariant set for a class of nonlinear systems with discontinuous right-hand sides in the sense of the Filippov solutions. When the Lyapunov function is Lipschitz continuous and regular, the Lyapunov theorem on finite-time stability with respect to a closed invariant set is presented.

On the Decomposition of a Bounded Closed Interval of the Real Line into Closed Sets

It has been shown by Sierpinski that a compact, Hausdorff, connected topological space (otherwise known as a continuum) cannot be decomposed into either a finite number of two or more disjoint, nonempty, closed sets or a countably infinite family of such sets. In particular, for a closed interval of the real line endowed with the usual topology, we see that we cannot partition it into a countably infinite number of disjoint, nonempty closed sets. On the positive side, however, one can certainly express such an interval as a union of c disjoint closed sets, where c is the cardinality of the real line. For example, a closed interval is surely the union of its points, each set consisting of a single point being closed. Surprisingly enough, except for a set of Lebesgue measure 0, these closed sets can be chosen to be perfect sets, i.e., closed sets every point of which is an accumulation point. They even turn out to be nowhere dense (containing no intervals). Such nowhere dense, perfect sets are sometimes called Cantor sets.

S-rough sets and knowledge separation

The conceptions of the knowledge screen generated by S-rough sets are given: f- screen and - screen , and then puts forward - filter theorem, - filter theorem of knowledge. At last, the applications of knowledge separation are given according to - screen and - screen.

On <i>M</i>-Asymmetric Semi-Open Sets and Semicontinuous Multifunctions in Bitopological Spaces

The purpose of this paper is to introduce the notions of m-asymmetric semiopen sets and M-asymmetric semicontinuous multifunctions defined between asymmetric sets satisfying certain minimal conditions in the framework of bitopological spaces. Some new characterizations of m-asymmetric semiopen sets and M-asymmetric semicontinuous multifunctions will be investigated and several fundamental properties will be obtained.

用变异FP-树改进CLOSET算法

频繁闭项集提供了频繁项集的一种完整、最小表示,对频繁闭项集的挖掘是近年来数据挖掘领域研究的热点,研究人员从不同角度对算法改进以提高算法的效率。基于频繁项集中共生项集的性质,提出无须进行子集检查的频繁闭项集挖掘方法,并设计一种变异的FP-树结构,利用FP-树结构来存储结点共生项集信息,以改进CLOSET算法,算法无须遍历结果集进行闭合性检查。实验表明,在支持度阈值减小,结果集变大时,改进算法的时间增长率比原有算法小。

L-拓扑空间中的SPC-连通性

研究了L-拓扑空间中的SPC-连通性.首先借助强半开集和强半闭集引入SPC-连通性的概念,并研究了SPC-连通性的等价刻画;其次讨论了SPC-连通性的一些性质以及证明了SPC-连通性是拓扑不变性;最后讨论了SPC-连通性与连通性之间的关系,得出:在L-拓扑空间中,SPC-连通一定是连通的,反之不成立,进而丰富和完善了L-拓扑空间中的连通性理论.

γ-（α,β）-Open Sets and γ-（α,β）-Ti Spaces

We introduce the concept of γ-(α, β)-open sets and the concepts of γ-(α, β)-T_i spaces(i = 0, 1, 2, 3, 4) based on the concept of(α, β)-open sets. We obtain some properties of γ-(α, β)-Tispaces. Furthermore, we study their essential characterization in topological space.

An Implanted Closed-loop Chip System for Heart Rate Control:System Design and Effects in Conscious Rats

Objective： To evaluate the efficiency of an implanted chip system for the control of heart rate （HR）. Methods： The HR was recorded in six conscious Sprague-Dawley （SD） rats. An implanted chip system was designed to regulate the HR by stimulating the right cervical vagus nerve according to the feedback of real time HR. Each rat was subjected to 30-min regulation and 30-min recovery. The change of HR during the regulation period was compared with the control. The ECG was recorded during the experiment for 24 h. Results： The ECG signals were successfully recorded during the experiment. The HR was significantly decreased during the period of regulation compared with control （-79.3 ± 34.5, P 〈 0.01, n = 6） and then recovered to normal after regulation. Conclusion： The described implanted chip system can regulate the HR to a designated set point.

Rough similarity degree and rough close degree in rough fuzzy sets and the applications

Based on rough similarity degree of rough sets and close degree of fuzzy sets, the definitions of rough similarity degree and rough close degree of rough fuzzy sets are given, which can be used to measure the similar degree between two rough fuzzy sets. The properties and theorems are listed. Using the two new measures, the method of clustering in the rough fuzzy system can be obtained. After clustering, the new fuzzy sample can be recognized by the principle of maximal similarity degree.

Theorems of Helly's Type for Partially-closed Half-spaces

In this article, under quite weaker conditions, theorems of Helly’s type for(not necessarily finite) family of partially closed half-spaces are presented in both analytic and geometric forms. Examples are also provided to show that these conditions cannot be omitted in general.

HB-Continuous Mappings in L-Topological Space

In this paper, we introduce and study the notion of HB-closed sets in L-topological space. Then, HB-convergence theory for L-molecular nets and L-ideals is established in terms of HB-closedness. Finally, we give a new definition of fuzzy H-continuous [1] which is called HB-continuity on the basis of the notion of H-bounded L-subsets in L-topological space. Then we give characterizations and properties by making use of HB-converges theory of L-molecular nets and L-ideals.

LEVEL SET输运方程的求解方法及其对气-液两相流运动界面数值模拟的影响

用数值模拟方法来研究气-液两相流动与传热现象是当今多相流领域的一个热门课题。由于两相流固有的复杂性,气-液两相流界面迁移现象的数值模拟一直是两相流研究中的一大难点。本文介绍了捕捉气-液两相流相界面运动的水平集方法(LevelSet)及其研究进展,介绍了求解LevelSet输运方程的3种方法,即一般差分格式、Superbee-TVD格式和Runge-Kutta法-5阶WENO组合格式。结合主流场的求解,分别用这3种方法对4种典型相界面在5种流场中的迁移特性进行了模拟计算,并对计算结果进行了比较和分析。结果表明,Runge-Kutta法-5阶WENO组合格式求解LevelSet输运方程的效果最好,在以后的计算中将主要采用这种组合格式来进行气-液相界面输运方程的求解。

高磷通过SET8-shRNA调控AKT/Caspase-3通路促进血管平滑肌细胞凋亡

目的探讨高磷通过SET8-shRNA调控AKT/Caspase-3通路促进大鼠血管平滑肌细胞(vascular smooth muscle cells,VSMCs)发生钙化的机制。方法将血管平滑肌细胞随机分为两组:正常组和高磷组(10 mmol/Lβ-甘油磷酸)。采用茜素红染色及钙含量检测血管平滑肌细胞钙化,采用实时荧光定量及Western blot检测SET8、AKT、Caspase-3 mRAN和蛋白表达情况。为验证SET8在细胞钙化中的作用,将大鼠VSMCs分为6组:正常组、空质粒组、SET8低表达组(转染SET8-shRNA质粒)、高磷组(10 mmol/Lβ-甘油磷酸)、空质粒+高磷组(高磷基础上转染SET8过表达的对照质粒)、SET8过表达+高磷组(高磷基础上转染SET8过表达质粒)。检测各组血管平滑肌细胞的钙化、凋亡及AKT、Caspase-3表达情况。为进一步验证AKT在VSMCs钙化中的作用,将血管平滑肌细胞分为3组:AKT抑制剂组(1μl DMSO+AKT抑制剂LY294002)、对照组(1μl DMSO)和正常组,检测AKT、Caspase-3 mRAN和蛋白表达。结果与正常组比较,高磷组细胞钙化结节增多,钙含量增加(P<0.05),STE8和AKT表达降低,Caspase-3表达升高(P<0.05)。与正常组和空质粒组比较,SET8-shRNA组细胞钙化和凋亡增多,AKT表达降低,Caspase-3表达升高(P<0.05);与高磷组和空质粒+高磷组比较,SET8过表达+高磷组细胞钙化和凋亡减少,AKT表达升高,Caspase-3表达降低(P<0.05)。与正常组和对照组比较,AKT抑制剂组AKT表达显著降低,Caspase-3表达显著升高(P<0.05)。结论高磷通过SET8-shRNA抑制AKT活化,进而促进Caspase-3表达,增加VSMCs凋亡,从而影响细胞钙化的发生。

B7-H3、葡萄糖6-磷酸脱氢酶及SET8蛋白在肾透明细胞癌中的表达及与预后的关系分析

目的分析B7-H3、葡萄糖6-磷酸脱氢酶(G6PD)及SET8蛋白在肾透明细胞癌中的表达及与预后的关系。方法收集81例肾透明细胞癌患者的肾透明细胞癌组织及正常组织,比较不同组织中B7-H3、G6PD及SET8蛋白阳性表达情况,随访2年分析肾透明细胞癌预后的影响因素。结果肾透明细胞癌组织中B7-H3、G6PD及SET8蛋白阳性表达率均明显高于正常组织,差异均有统计学意义(P﹤0.01)。多因素分析结果显示,肿瘤直径≥5 cm、TNM分期为Ⅲ-Ⅳ期、有淋巴结转移、细胞分级为G2-3级、B7-H3阳性、G6PD阳性、SET8阳性均为影响肾透明细胞癌患者预后的独立危险因素(P﹤0.05)。结论B7-H3、G6PD及SET8蛋白在肾透明细胞癌中均高表达,在临床中检测三者表达情况对患者预后评估有重要参考价值。

基于SET/MOS混合结构的4-2编码器设计

基于单电子晶体管(SET)的库仑振荡效应、多栅输入特性和相移特性,实现了SET/MOS混合结构的或门逻辑,并利用该或门逻辑实现了4-2编码器。利用HSPICE对所设计电路进行仿真验证,仿真结果表明该电路能够实现4-2编码器的编码功能。与纯CMOS编码器相比,该SET/MOS混合电路仅由2个PMOS管、2个NMOS管和2个SET构成,具有结构简单、尺寸小、集成度高的优点。