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Effects of β-Amino Butyric Acid Induced Rice Blast Resistance on Reactive Oxygen Metabolism 被引量:5
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作者 李莉 郭晓丽 +5 位作者 刘晓梅 温嘉伟 张秀容 孙辉 赵宇 任金平 《Agricultural Science & Technology》 CAS 2009年第3期112-114,共3页
[Objective] This study was to understand the effects of β-Amino butyric acid(abbreviated as BABA) induced rice blast resistance on reactive oxygen metabolism. [Method] Using the cultivar Chaochan 2 that is highly sus... [Objective] This study was to understand the effects of β-Amino butyric acid(abbreviated as BABA) induced rice blast resistance on reactive oxygen metabolism. [Method] Using the cultivar Chaochan 2 that is highly susceptible to disease as experimental material, the changes of catalase(CAT), and superoxide dismutase(SOD) and MDA activities in rice treated by BABA were investigated. [Result] In rice plants treated by BABA, the activities of CAT and SOD increased, meanwhile the MDA content also rose to some extent, resulting in the disease resistance to rice blast. [Conclusion] By influencing reactive oxygen metabolism, BABA endows rice plants with resistance to rice blast. BABA is safe to environment and has highly resistance-inducing capacity, it could be generalized in production. 展开更多
关键词 β-amino butyric acid Rice blast Induced resistance
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Dietary <i>γ</i>-Aminobutyric Acid Shortens the Life Span of Stroke-Prone Spontaneously Hypertensive Rats
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作者 Nakamichi Watanabe Naomi Washio 《Food and Nutrition Sciences》 2011年第4期301-303,共3页
Dietary γ-amino butyric acid (GABA) has been suggested to decrease systolic blood pressure. This study aimed to ex-amine the effects of dietary GABA on the life span of stroke-prone spontaneously hypertensive rats (S... Dietary γ-amino butyric acid (GABA) has been suggested to decrease systolic blood pressure. This study aimed to ex-amine the effects of dietary GABA on the life span of stroke-prone spontaneously hypertensive rats (SHRSPs). In this study, life span was determined for SHRSPs provided 1% NaCl solution or 0.01% GABA in 1% NaCl solution as drinking water. The life span of the GABA-fed group (76.3 ± 1.65 days) was significantly shorter than that of the control group (81.6 ± 0.88 days). The results of this study may not be applicable to humans. Future studies will be necessary to elucidate the mechanism underlying this phenomenon. 展开更多
关键词 γ-amino butyric acid Stroke-Prone Spontaneously HYPERTENSIVE Rat Life Span
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The Dominant Glutamic Acid Metabolic Flux to Produce γ-Amino Butyric Acid over Proline in Nicotiana tabacum Leaves under Water Stress Relates to its Significant Role in Antioxidant Activity 被引量:27
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作者 Cuili Liu Li Zhao Guanghui Yu 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2011年第8期608-618,共11页
γ-Amino butyric acid (GABA) and proline play a crucial role in protecting plants during various environmental stresses. Their synthesis is from the common precursor glutamic acid, which is catalyzed by glutamate de... γ-Amino butyric acid (GABA) and proline play a crucial role in protecting plants during various environmental stresses. Their synthesis is from the common precursor glutamic acid, which is catalyzed by glutamate decarboxylase and △1-pyrroline-5-carboxylate synthetase respectively. However, the dominant pathway under water stress has not yet been established. To explore this, excised tobacco leaves were used to simulate a water-stress condition. The results showed GABA content was much higher than that of proline in leaves under water-deficit and non-water-deficit conditions. Specifically, the amount of GABA significantly increased compared to proline under continuous water loss for 16 h, indicating that GABA biosynthesis is the dominant pathway from glutamic acid metabolism under these conditions. Quantitative reverse transcription polymerase chain reaction and protein Western gel-blot analysis further confirmed this. To explore the function of GABA accumulation, a system producing superoxide anion (O 2 - ), peroxide hydrogen (H 2 O 2 ), and singlet oxygen ( 1 O 2 ) was employed to investigate the scavenging role on free-radical production. The results demonstrated that the scavenging ability of GABA for O 2 - , H 2 O 2 , and 1 O 2 was significantly higher than that of proline. This indicated that GABA acts as an effective osmolyte to reduce the production of reactive oxygen species under water stress. 展开更多
关键词 glutamic acid PROLINE reactive oxygen species water stress γ-amino butyric acid.
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Contents Variation Analysis ofγ-Amino Butyric Acid in Semen sojae praeparatum Fermentation Using Online Pre-Column Derivatization-HPLC 被引量:2
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作者 WENG Meizhi HE Jing +5 位作者 REN Jiaxiu LIANG Yonghong CHEN Qingfeng SU Mingsheng WANG Liyuan XIE Xiaomei 《Wuhan University Journal of Natural Sciences》 CAS CSCD 2021年第1期55-61,共7页
This paper reported the contents variation analysis ofγ-amino butyric acid(GABA)in Semen sojae praeparatum(SSP)which is a famous traditional Chinese medicine.High performance liquid chromatography(HPLC)was used and G... This paper reported the contents variation analysis ofγ-amino butyric acid(GABA)in Semen sojae praeparatum(SSP)which is a famous traditional Chinese medicine.High performance liquid chromatography(HPLC)was used and GABA was derivatized by online pre-column derivatization with o-phthalaldehyde(OPA).To validate this method,the precision,stability,repeatability and recovery were discussed.In the concentration range from 0.0125 to 0.400 mg/m L,the calibration curve for GABA was linear and the regression equation was obtained with correlation coefficient(R2)of 0.9999.Relatively high levels of GABA exist in SSP and the content changes of GABA at different time points during the fermenting process were detected.At the"yellow cladding"stage,GABA level was very low or even undetectable;the"secondary fermentation"stage witnessed a rapid increase of GABA content to 1.39-5.52 mg/g,which remained stable after 18 days of"secondary fermentation".This study demonstrated that GABA was generated at the"secondary fermentation"stage,revealing the significance and rationality of the"secondary fermentation"stage in the fermenting process of SSP.On the other hand,it suggested the downside of taking soy isoflavones as the only measurement in existing quality assessment and optimization approach for the fermenting process of SSP. 展开更多
关键词 Semen sojae praeparatum γ-amino butyric acid(GABA) "secondary fermentation"process online pre-column derivatization-high performance liquid chromatography
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Fermentation of groundnut brittle by Lactococcus lactis producesγ-amino butyric acid and enhances nutritional quality and safety 被引量:1
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作者 Vibhuti Batra Hitashi Lomash Abhijit Ganguli 《Food Quality and Safety》 SCIE 2018年第2期83-88,共6页
Objectives:The study aimed to evaluate the anti-nutrient reductions andγ-amino butyric acid(GABA)enrichments of chikki(peanut brittle),a popular of traditional snack food,using Lactococcus lactis subsp.lactis.The nut... Objectives:The study aimed to evaluate the anti-nutrient reductions andγ-amino butyric acid(GABA)enrichments of chikki(peanut brittle),a popular of traditional snack food,using Lactococcus lactis subsp.lactis.The nutritional,storage,and sensorial analysis in order to understand the safety and functionality of chikki following fermentation were attempted.Materials and Methods:Partial fermentations of chikki were carried out using overnight grown culture of L.lactis samples.The fermented chikki were further analysed for GABA and anti-nutrients.The antioxidant profile,protein,and sugar were also analysed.The storage studies were carried on up to 2 months for functional property evaluations.Results:Fermentations at 37°C,pH of 5,with 1%inoculum and incubation for 24 h were optimal conditions,and resulted in the GABA concentration of 816 mg/g,respectively,and the GABA concentration did not change significantly(P>0.05)upon storage for upto a period of 2 months.Analysis of the fermented chikki revealed a slightly higher level of phenolic,flavonoid,protein,and sugar contents as compared to those which were not subjected to fermentation.The results of sensorial analysis showed an overall general acceptability on a 5-point hedonic scale to be 8.5±0.01(before storage)and 8.03±0.01(after storage).The fermented chikki also possessed antioxidant properties and significantly(P<0.05)low levels of phytates with complete reductions of other anti-nutrients.Conclusions:Traditionally prepared/manufactured chikki lacks GABA,possesses notable levels of anti-nutrients with lower phenolics,flavonoids,as well as antioxidants.Our study suggested a simple preparation of GABA could enrich the popular ethnic snack through fermentation by L.lactis subsp.lactis.The developed snack is acceptable,economical with good shelf life,and has substantially reduced levels of anti-nutrients originating from groundnuts affording consumer safety. 展开更多
关键词 γ-amino butyric acid ANTI-NUTRIENTS ethnic snack Lactococcus lactis
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Role of short chain fatty acids in gut health and possible therapeutic approaches in inflammatory bowel diseases 被引量:1
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作者 Marcos Antônio Ferreira Caetano Patricia Castelucci 《World Journal of Clinical Cases》 SCIE 2022年第28期9985-10003,共19页
Inflammatory bowel diseases(IBDs) are characterized by inflammation in the gastrointestinal tract and include Ulcerative Colitis and Crohn’s Disease.These diseases are costly to health services,substantially reduce p... Inflammatory bowel diseases(IBDs) are characterized by inflammation in the gastrointestinal tract and include Ulcerative Colitis and Crohn’s Disease.These diseases are costly to health services,substantially reduce patients’ quality of life,and can lead to complications such as cancer and even death.Symptoms include abdominal pain,stool bleeding,diarrhea,and weight loss.The treatment of these diseases is symptomatic,seeking disease remission.The intestine is colonized by several microorganisms,such as fungi,viruses,and bacteria,which constitute the intestinal microbiota(IM).IM bacteria promotes dietary fibers fermentation and produces short-chain fatty acids(SCFAs) that exert several beneficial effects on intestinal health.SCFAs can bind to G protein-coupled receptors,such as GPR41 and GPR43,promoting improvements in the intestinal barrier,anti-inflammatory,and antioxidant effects.Thus,SCFAs could be a therapeutic tool for IBDs.However,the mechanisms involved in these beneficial effects of SCFAs remain poorly understood.Therefore,this paper aims to provide a review addressing the main aspects of IBDs,and a more detailed sight of SCFAs,focusing on the main effects on different aspects of the intestine with an emphasis on IBDs. 展开更多
关键词 Ulcerative colitis Crohn’s disease Short-chain fatty acid butyrATE Inflammatory bowel diseases Free fatty acid receptor
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Emergence of taurine as a therapeutic agent for neurological disorders
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作者 Ashok Jangra Priyanka Gola +13 位作者 Jiten Singh Pooja Gond Swarnabha Ghosh Mahesh Rachamalla Abhijit Dey Danish Iqbal Mehnaz Kamal Punya Sachdeva Saurabh Kumar Jha Shreesh Ojha Dinesh Kumar Niraj Kumar Jha Hitesh Chopra Shing Cheng Tan 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第1期62-68,共7页
Taurine is a sulfur-containing,semi-essential amino acid that occurs naturally in the body.It alternates between inflammation and oxidative stress-mediated injury in various disease models.As part of its limiting func... Taurine is a sulfur-containing,semi-essential amino acid that occurs naturally in the body.It alternates between inflammation and oxidative stress-mediated injury in various disease models.As part of its limiting functions,taurine also modulates endoplasmic reticulum stress,Ca^(2+)homeostasis,and neuronal activity at the molecular level.Taurine effectively protects against a number of neurological disorders,including stro ke,epilepsy,cerebral ischemia,memory dysfunction,and spinal cord injury.Although various therapies are available,effective management of these disorders remains a global challenge.Approximately 30 million people are affected worldwide.The design of taurine fo rmation co uld lead to potential drugs/supplements for the health maintenance and treatment of central nervous system disorders.The general neuroprotective effects of taurine and the various possible underlying mechanisms are discussed in this review.This article is a good resource for understanding the general effects of taurine on various diseases.Given the strong evidence for the neuropharmacological efficacy of taurine in various experimental paradigms,it is concluded that this molecule should be considered and further investigated as a potential candidate for neurotherapeutics,with emphasis on mechanism and clinical studies to determine efficacy. 展开更多
关键词 antioxidant EPILEPSY γ-amino butyric acid neurodegenerative disorders NEUROPROTECTION oxidative stress spinal cord injury TAURINE
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肠易激综合征大鼠肠神经系统内γ-氨基丁酸及其受体的表达 被引量:4
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作者 陈鹏 胡立华 +2 位作者 张艳杰 常英娟 张鉴颖 《中国现代医生》 2012年第21期15-17,共3页
目的通过研究γ-氨基丁酸(GABA)及其受体在便秘型肠易激综合征(C-IBS)大鼠模型肠神经系统中表达的变化,探讨GABA在肠易激综合征(IBS)发病机制中的可能作用。方法通过0~4℃冰水灌胃方法建立C-IBS大鼠模型,分别取模型组和正常对照组大鼠... 目的通过研究γ-氨基丁酸(GABA)及其受体在便秘型肠易激综合征(C-IBS)大鼠模型肠神经系统中表达的变化,探讨GABA在肠易激综合征(IBS)发病机制中的可能作用。方法通过0~4℃冰水灌胃方法建立C-IBS大鼠模型,分别取模型组和正常对照组大鼠的回盲部以及距肛门3cm处结肠的标本,进行灌注、固定、切片。用抗谷氨酸脱羧酶(GAD)、GABAA受体、GABAB受体抗体进行免疫组织化学染色,应用病理图像分析系统对不透光率密度值进行半定量分析。结果与正常大鼠相比,C-IBS大鼠模型回盲部及距肛门3cm处结肠肌层中GAD免疫反应性明显降低(P<0.05),GABAA受体免疫反应性无明显变化(P>0.05),GABAB受体免疫反应性明显降低(P<0.05)。结论 GABA可能通过其受体间接地参与IBS的病理生理过程。 展开更多
关键词 肠易激综合征 肠神经系统 Γ-氨基丁酸 Γ-氨基丁酸受体
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谷氨酸能和γ氨基丁酸能系统与情感障碍 被引量:21
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作者 周璇 王雪琦 《中国神经科学杂志》 CSCD 2003年第2期130-133,共4页
越来越多证据表明 ,谷氨酸能和γ氨基丁酸能 (GABA)系统可能参与了抑郁症的发病过程。如抗抑郁药可直接或间接降低谷氨酸NMDA受体的功能 ;NMDA受体拮抗剂具有抗抑郁作用 ;降低谷氨酸能活性或抑制谷氨酸受体相关信号转导的药物具有抗躁... 越来越多证据表明 ,谷氨酸能和γ氨基丁酸能 (GABA)系统可能参与了抑郁症的发病过程。如抗抑郁药可直接或间接降低谷氨酸NMDA受体的功能 ;NMDA受体拮抗剂具有抗抑郁作用 ;降低谷氨酸能活性或抑制谷氨酸受体相关信号转导的药物具有抗躁狂作用。磁共振波谱的研究提示单相抑郁与皮质GABA浓度的减少有关 ;抗抑郁药和情绪稳定剂可提高皮质的GABA浓度 ,并改善情感障碍病人GABA缺乏的状况。因此研制作用于特异的谷氨酸受体和GABA受体的药物可能是未来抗抑郁药研究的热点。 展开更多
关键词 谷氨酸 γ氨基丁酸能系统 情感障碍 NMDA受体 Γ氨基丁酸 抑郁症 双相性情感障碍
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γ-氨基丁酸能去抑制对脊髓细胞外信号调节激酶2的激动作用 被引量:2
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作者 时蕾 刘燕妮 +4 位作者 索占伟 曹静 李帅 许英明 胡晓东 《中国药理学与毒理学杂志》 CAS CSCD 北大核心 2012年第3期294-298,共5页
目的探讨脊髓γ-氨基丁酸(GABA)能去抑制对细胞外信号调节激酶1/2(ERK1/2)活性的影响及其与痛行为改变的关系。方法正常小鼠鞘内注射(ith)比扣扣灵碱(荷包牡丹碱,bicuculline)50 ng.g-1(5μl)模拟GABA能去抑制,左后足底sc给予弗氏完全... 目的探讨脊髓γ-氨基丁酸(GABA)能去抑制对细胞外信号调节激酶1/2(ERK1/2)活性的影响及其与痛行为改变的关系。方法正常小鼠鞘内注射(ith)比扣扣灵碱(荷包牡丹碱,bicuculline)50 ng.g-1(5μl)模拟GABA能去抑制,左后足底sc给予弗氏完全佐剂制备炎性疼痛模型,小鼠ith给予GABAA受体激动剂地西泮0.5μg.g-1(5μl)或ERK1/2抑制剂PD-98059 0.25μg.g-1(5μl)后,测定ERK1/2活性和小鼠缩足阈值。结果与正常对照组的缩足阈值(1.24±0.07)g相比,正常小鼠ith给予比扣扣灵碱50 ng.g-1的缩足阈值显著降低〔(0.42±0.17)g,P<0.05〕,给予PD-98059 0.25μg.g-1后缩足阈值显著升高〔(1.29±0.37)g,P<0.05〕。与炎性疼痛模型组的缩足阈值(0.28±0.06)g相比,炎症小鼠ith给予地西泮0.5μg.g-1的缩足阈值显著升高〔(0.99±0.12)g,P<0.05〕,且给予PD-98059 0.25μg.g-1后缩足阈值也显著升高〔(0.97±0.17)g,P<0.05〕。Western免疫印迹结果显示,与正常对照组相比,比扣扣灵碱显著提高ERK2的磷酸化水平〔(152±24)%,P<0.05〕。并且弗氏完全佐剂也可提高小鼠脊髓ERK2的磷酸化水平〔(163±42)%,P<0.05〕,ith给予地西泮0.5μg.g-1,则显著降低CFA诱发的小鼠脊髓ERK2的磷酸化水平〔(91±34)%,P<0.05〕,同时地西泮和PD-98059有效缓解炎性疼痛症状。结论 GABA能去抑制对脊髓背角ERK2有激活作用,并与炎性疼痛的形成有关。 展开更多
关键词 受体 γ-氨基丁酸 炎性疼痛 细胞外信号调节激酶1/2 脊髓背角
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γ-氨基丁酸及其受体在大鼠纹状体边缘区的表达 被引量:3
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作者 包新民 舒斯云 王虹 《第一军医大学学报》 CSCD 北大核心 2002年第11期961-965,共5页
目的观察γ-氨基丁酸(GABA)及其受体GABARB1mRNA在纹状体边缘区的表达,探讨GABA对边缘区学习记忆功能的调控。方法应用免疫细胞化学方法观察GABA及其合成酶谷氨酸脱羧酶(GAD)在纹状体边缘区的分布。用分子原位杂交方法观察GABA受体GABAR... 目的观察γ-氨基丁酸(GABA)及其受体GABARB1mRNA在纹状体边缘区的表达,探讨GABA对边缘区学习记忆功能的调控。方法应用免疫细胞化学方法观察GABA及其合成酶谷氨酸脱羧酶(GAD)在纹状体边缘区的分布。用分子原位杂交方法观察GABA受体GABARB1mRNA在纹状体边缘区内的表达。结果在纹状体边缘区内可见密集的GABA及GAD免疫阳性纤维及少量胞体,在皮层、海马等处也可见阳性纤维及胞体。边缘区内可见许多GABARB1mRNA表达阳性的细胞,尾壳核内只有少量GABARB1mRNA阳性细胞分布,皮层、海马等处也呈GABARB1mRNA阳性表达。结论证实边缘区存在着GABA及其受体的表达,表明存在着抑制性氨基酸对边缘区的调控,推测GABA通过抑制突触前递质的释放及调控其他神经递质来影响边缘区的学习记忆功能。 展开更多
关键词 表达 Γ-氨基丁酸 纹状体边缘区 生理学 免疫组织化学 GABA 学习记忆功能 Γ-氨基丁酸受体 分子原位杂交
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神经甾体在肝性脑病中的研究进展
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作者 王小英 谢瑞霞 +1 位作者 张建刚 张德奎 《世界华人消化杂志》 CAS 北大核心 2014年第33期5086-5091,共6页
肝性脑病(hepatic encephalopathy,HE)是由慢性或急性肝脏疾病引起的神经精神症候群的一种医学现象.神经甾体是以胆固醇及其前体为原料,由中枢神经系统多种细胞(神经元、少突胶质细胞、星形胶质细胞)合成.神经甾体通过基因和非基因效应... 肝性脑病(hepatic encephalopathy,HE)是由慢性或急性肝脏疾病引起的神经精神症候群的一种医学现象.神经甾体是以胆固醇及其前体为原料,由中枢神经系统多种细胞(神经元、少突胶质细胞、星形胶质细胞)合成.神经甾体通过基因和非基因效应参与HE的病理生理.一方面,神经甾体结合并调节不同的神经元膜受体,包括γ-氨基丁酸A受体(gamma amino butyric acid-A receptor,GABA-A)、N-甲基-D-天冬氨酸受体(N-methyl-Daspartic acid receptor,NMDA)、5-羟色胺3(5-hydroxytryptamine 3,5-HT3)受体及阿片受体等;另一方面,神经甾体结合于细胞内受体来调节基因表达.值得一提的是,神经甾体通过抑制海马突触活性的长时程增强效应(long-term potentiation,LTP)参与HE的病理生理.神经甾体有望为肝性脑病治疗提供新的途径. 展开更多
关键词 肝性脑病 神经甾体 转运蛋白 Γ-氨基丁酸A受体
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单侧迷路切除后γ-氨基丁酸A受体亚单位在大鼠小脑绒球中的表达
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作者 刘珺 孔维佳 《听力学及言语疾病杂志》 CAS CSCD 2008年第6期491-494,共4页
目的观察单侧迷路切除术后大鼠小脑绒球内γ-氨基丁酸A(gamma—amino butyric acid A,GABAA)受体α3、β1、δ亚单位的表达变化。方法成年雄性Wistar大鼠24只,随机分为迷路破坏组(18只)和对照组(6只),前者破坏单侧迷路,对照... 目的观察单侧迷路切除术后大鼠小脑绒球内γ-氨基丁酸A(gamma—amino butyric acid A,GABAA)受体α3、β1、δ亚单位的表达变化。方法成年雄性Wistar大鼠24只,随机分为迷路破坏组(18只)和对照组(6只),前者破坏单侧迷路,对照组手术方式相同但保持迷路完好。通过免疫组织化学法检测术后不同存活时间(术后1、3、7天)两组动物小脑绒球内GABAA受体α3、β1、δ亚单位的表达变化。结果单侧迷路切除术后大鼠术侧小脑绒球内GABAA受体β1、δ亚单位增加,术后1天最多,此后3~7天处于下降趋势,7天时和对照组比较表达无差异。α3亚单位在小脑绒球的染色很弱,各组两侧比较及术后1、3、7天与对照组比较均无明显差异。结论单侧迷路切除后大鼠术侧小脑绒球内GABAA受体β1、δ亚单位增加;双侧前庭中枢神经元的静息放电的平衡可能与GABAA受体β1、δ亚单位绒球内的增加有关;α3亚单位可能并非GABAA受体在小脑绒球主要的结构与功能证单侍。 展开更多
关键词 绒球 前庭代偿 Γ-氨基丁酸A受体 大鼠
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邻苯二甲酸二丁酯对原代培养海马神经元的毒性及机制 被引量:2
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作者 李洋 李秀娟 +4 位作者 谢明丹 程莉 陈恒胜 孙红 蒋莉 《南方医科大学学报》 CAS CSCD 北大核心 2020年第2期225-232,共8页
目的本研究探讨邻苯二甲酸二丁酯(DBP)暴露对原代培养海马神经元的神经毒性及可能机制。方法海马神经元原代培养4 d后将海马神经元暴露在含有终浓度为0.0(对照)、1 g/LDBP的培养基中,选取染毒24、48、96 h 3个时相点,免疫荧光及透射电... 目的本研究探讨邻苯二甲酸二丁酯(DBP)暴露对原代培养海马神经元的神经毒性及可能机制。方法海马神经元原代培养4 d后将海马神经元暴露在含有终浓度为0.0(对照)、1 g/LDBP的培养基中,选取染毒24、48、96 h 3个时相点,免疫荧光及透射电子显微镜下观察DBP染毒后海马神经元轴突及超微结构的形态变化;膜片钳测定海马神经元的动作电位;cck-8检测DBP染毒后海马神经元活性。Western blot测定脑源性神经营养因子(BDNF)、神经肽Y(NPY)、雌激素受体β(ERβ)核酸及蛋白的表达情况。高效液相色谱串联质谱检测神经递质GABA的释放。结果DBP染毒96 h后神经元网络稀疏、轴突长度变短(P<0.01),电镜下细胞核呈圆形,染色质聚集,细胞质空泡化;膜片钳测定发现DBP染毒96 h后细胞出现去极化漂移、放电频率增加(P<0.01);cck-8检测发现DBP染毒24、48、96 h的细胞活性均低于同时间点正常对照组(P<0.01)。DBP染毒48、96 h的ERβ、BDNF、NPY蛋白表达显著低于对照组(P<0.05)。DBP染毒96 h后神经递质GABA的释放显著低于对照组(P<0.05)。结论DBP染毒后可导致海马神经细胞形态受损,并导致功能改变,这可能与神经递质GABA参与的ERβ-BDNF-NPY信号通道受阻相关。 展开更多
关键词 DBP 海马神经元 雌激素受体 脑源性神经营养因子 神经肽Y Γ-氨基丁酸
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Cyclooxygenase-2 inhibitor inhibits hippocampal synaptic reorganization in pilocarpine-induced status epilepticus rats 被引量:6
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作者 Hai-ju ZHANG Ruo-peng SUN +2 位作者 Ge-fei LEI Lu YANG Chun-xi LIU 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2008年第11期903-915,共13页
Objective: To examine modulations caused by cyclooxygenase-2 (COX-2) inhibitors on altered microenvironments and overbalanced neurotransmitters in pilocarpine-induced epileptic status rats and to investigate possib... Objective: To examine modulations caused by cyclooxygenase-2 (COX-2) inhibitors on altered microenvironments and overbalanced neurotransmitters in pilocarpine-induced epileptic status rats and to investigate possible mechanisms. Methods: Celecoxib (a COX-2 inhibitor) was administered 45 min prior to pilocarpine administration. The effects of COX-2 inhibitors on mlPSCs (miniature GABAergic inhibitory postsynaptic currents) of CA3 pyramidal cells in the hippocampus were recorded. Expressions of COX-2, c-Fos, newly generated neurons, and activated microgliosis were analyzed by immunohistochemistry, and expressions of c^-subunit of y-amino butyric acid (GABAA) receptors and mitogen-activated protein kinase/extracellular signal-regulated protein kinase (MAPK/ERK) activity were detected by Western blotting. Results: Pretreatment with celecoxib showed protection against pilocarpine-induced seizures. Celecoxib prevented microglia activation in the hilus and inhibited the abnormal neurogenesis and astrogliosis in the hippocampus by inhibiting MAPK/ERK activity and c-Fos transcription. Celecoxib also up-regulated the expression of GABAA receptors. NS-398 (N-2-cyclohexyloxy-4-nitrophenyl-methanesulfonamide), another COX-2 inhibitor, enhanced the frequency and decay time of mIPSCs. Conclusion: The COX-2 inhibitor celecoxib decreased neuronal excitability and prevented epileptogenesis in pilocarpine-induced status epilepticus rats. Celecoxib regulates synaptic reorganization by inhibiting astrogliosis and ectopic neurogenesis by attenuating MAPK/ERK signal activity, mediated by a GABAergic mechanism. 展开更多
关键词 EPILEPTOGENESIS Cyclooxygenase-2 (COX-2) NEUROGENESIS Microglia 3 -amino butyric acid (GABA) c-Fos Mitogen-activated protein kinase/extracellular signal-regulated protein kinase (MAPK/ERK) Spontaneousrecurrent seizure
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丁酸盐对糖尿病肾病小鼠肾损伤的保护作用及机制 被引量:5
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作者 叶凯丽 黄诗琴 +1 位作者 胡婷 赵艳玲 《温州医科大学学报》 CAS 2023年第1期42-48,共7页
目的:探讨丁酸盐对2型糖尿病肾病(DN)小鼠肾损伤的保护作用及其机制。方法:将雄性db/db小鼠随机分为模型组(DN)、丁酸钠500 mg/(kg·d)组(NaB1)和丁酸钠1000 mg/(kg·d)组(NaB2),同周龄雄性db/m作为对照组(NC)。NaB1组和NaB2组... 目的:探讨丁酸盐对2型糖尿病肾病(DN)小鼠肾损伤的保护作用及其机制。方法:将雄性db/db小鼠随机分为模型组(DN)、丁酸钠500 mg/(kg·d)组(NaB1)和丁酸钠1000 mg/(kg·d)组(NaB2),同周龄雄性db/m作为对照组(NC)。NaB1组和NaB2组每日丁酸钠溶液灌胃,NC组和DN组予等体积0.9%氯化钠溶液,连续灌胃8周后处死小鼠。留取肾组织进行苏木素伊红(HE)染色和过碘酸-希夫(PAS)染色;分别提取肾组织mRNA和蛋白进行实时荧光定量聚合酶链反应(RT-qPCR)和蛋白质印迹(Western blot)检测;采用相关试剂盒检测尿肌酐、尿微量白蛋白(m ALB)、肾组织白细胞介素-6(IL-6)、血肌酐(SCr)等相关指标。结果:与NC组小鼠比,DN组小鼠精神萎靡,多饮多食多尿症状明显,体质量、血糖显著升高、尿白蛋白/肌酐比值(UACR)明显增高(P<0.05)。丁酸钠治疗8周后,小鼠精神饮食状况改善,血糖、UACR下降、肾组织IL-6水平下降,GLP-1R、AMPK、PGC-1α、MFN2和OPA1 mRNA表达增加(P<0.05)。结论:丁酸钠通过调节DN小鼠的AMPK和GLP-1R发挥肾脏保护作用。 展开更多
关键词 丁酸盐 短链脂肪酸 糖尿病肾病 AMPK/PGC-1α DB/DB小鼠
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丁酸对脂肪代谢的调节及其作用机制 被引量:3
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作者 徐进 王劼 +1 位作者 舒鼎铭 王旭溟 《动物营养学报》 CAS CSCD 北大核心 2022年第6期3495-3502,共8页
丁酸作为肠道菌群发酵产物之一,不仅可为生物体提供能量,还能以信号分子形式调节体内能量和物质代谢。近年来,在饲粮中添加丁酸能够有效减少动物过多脂肪沉积而受到广泛关注,然而一些研究发现丁酸表现出促进脂肪合成的作用。丁酸一方面... 丁酸作为肠道菌群发酵产物之一,不仅可为生物体提供能量,还能以信号分子形式调节体内能量和物质代谢。近年来,在饲粮中添加丁酸能够有效减少动物过多脂肪沉积而受到广泛关注,然而一些研究发现丁酸表现出促进脂肪合成的作用。丁酸一方面作为脂肪合成的底物,另一方面以信号分子形式即通过结合G蛋白偶联受体(GPCRs)或者抑制组蛋白去乙酰化酶(HDACs)的活性来调节脂肪代谢。本文主要从这2方面来探讨丁酸与脂肪代谢之间的联系,为畜禽养殖中如何减少过多脂肪沉积或抑制肥胖提供理论参考。 展开更多
关键词 丁酸 脂肪代谢 G蛋白偶联受体 组蛋白去乙酰化酶 肥胖
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ISOLATION AND IDENTIFICATION OF γ-AMINOBU-TYRIC ACID AND QUANTITATIVE ANALYSIS OF ITS AMINO ACID CONTENTS FROM PANAX GINSENG
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作者 杨柳 叶蕴华 +1 位作者 袁洪生 邢其毅 《Chinese Science Bulletin》 SCIE EI CAS 1991年第20期1706-1710,共5页
Panax ginseng (C. A. Meyer) is the most popular Chinese herb and has been used for medicine for centuries in China and some other Asian countries. Previous studies of this herb were mostly concentrated on the constitu... Panax ginseng (C. A. Meyer) is the most popular Chinese herb and has been used for medicine for centuries in China and some other Asian countries. Previous studies of this herb were mostly concentrated on the constituents soluble in organic solvents, but relatively less on those soluble in water. We think that more attention should be paid to the latter be- 展开更多
关键词 PANAX GINSENG γ-amino butyric acid AMINO acid analysis.
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Sodium butyrate protects against lipopolysaccharideinduced liver injury partially via the GPR43/b-arrestin-2/NF-кB network 被引量:2
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作者 Qian-Jiang Luo Mei-Xing Sun +7 位作者 Yun-Wei Guo Si-Wei Tan Xiao-Ying Wu Kodjo-Kunale Abassa Li Lin Hui-Ling Liu Jie Jiang Xiu-Qing Wei 《Gastroenterology Report》 SCIE EI 2021年第2期154-165,I0002,共13页
Background Butyrate acts as a regulator in multiple inflammatory organ injuries.However,the role of butyrate in acute liver injury has not yet been fully explored.In the present study,we aimed to investigate the assoc... Background Butyrate acts as a regulator in multiple inflammatory organ injuries.However,the role of butyrate in acute liver injury has not yet been fully explored.In the present study,we aimed to investigate the association between butyrate and lipopolysaccharide(LPS)-induced acute liver injury and the signaling pathways involved.Methods LPS-induced acute liver injury was induced by intraperitoneal injection of LPS(5 mg/kg)in G-protein-coupled receptor 43(GPR43)-knockout(KO)and wild-type female C57BL/6 mice.Sodium butyrate(500mg/kg)was administered intraperitoneally 30min prior to LPS exposure.Liver injury was detected by serum markers,tissue morphology,and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL).Pro-inflammatory-factor levels were detected by enzyme-linked immunosorbent assay and real-time polymerase chain reaction(RT-PCR).Cell models were first treated with sodium butyrate(4μmol/mL),followed by LPS(1μg/mL)half an hour later in GPR43 small interfering RNA(siRNA)-transfected or control RAW264.7 cells.Cell-inflammation status was evaluated through detecting pro-inflammatory-factor expression by RT-PCR and also through checking toll-like receptor 4/nuclear factor-кB(TLR4/NF-кB)-element levels including TLR4,TRAF6,IKKb,IкBα,phospho-IкBα,p65,and phospho-p65 by Western blot.The interaction between GPR43 and barrestin-2 was tested by co-immunoprecipitation.Results Sodium butyrate reversed the LPS-induced tissue-morphology changes and high levels of serum alanine aminotransferase,aspartate transaminase,myeloperoxidase,TUNEL,and pro-inflammatory cytokines such as tumor necrosis factor-a and interleukin-6.The ameliorating effect of sodium butyrate was weakened in GPR43-KO mice and GPR43 siRNA RAW264.7 cells,compared with those of GPR43-positive controls.Sodium butyrate downregulated some elements of the TLR4/NF-кB pathway,including phospho-IкBαand phospho-p65,in RAW264.7 cells.Increased interactions between GPR43 and b-arrestin-2,and between b-arrestin-2 and IrBa were observed.Conclusion Sodium butyrate significantly attenuated LPS-induced liver injury by reducing the inflammatory response partially via the GPR43/b-arrestin-2/NF-кB signaling pathway. 展开更多
关键词 sodium butyrate short-chain fatty acids lipopolysaccharide-induced liver injury G-protein-coupled receptor 43 b-arrestin-2 NF-кB
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γ-氨基丁酸(GABA)及其A型受体π亚基在植入前小鼠子宫的动态表达 被引量:3
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作者 刘真真 罗文萍 +1 位作者 谭冬梅 谭毅 《生殖与避孕》 CAS CSCD 北大核心 2011年第6期364-368,共5页
目的:探讨γ-氨基丁酸(GABA)及其A型受体π亚基在胚胎植入中的作用。方法:①Real-time PCR方法检测π亚基mRNA在妊娠小鼠第1 ̄5日子宫组织的表达变化。②免疫组织化学方法检测GABA和π亚基在妊娠小鼠第1日、第4日子宫组织的表达。结果:... 目的:探讨γ-氨基丁酸(GABA)及其A型受体π亚基在胚胎植入中的作用。方法:①Real-time PCR方法检测π亚基mRNA在妊娠小鼠第1 ̄5日子宫组织的表达变化。②免疫组织化学方法检测GABA和π亚基在妊娠小鼠第1日、第4日子宫组织的表达。结果:①π亚基mRNA在妊娠小鼠第1 ̄5日子宫组织均有表达,并随妊娠进程逐渐降低,第1日、第2日表达较强,第3日开始下降,第4日、第5日显著下降(P<0.01),且第5日植入位点组织明显低于植入位点旁组织(P<0.01);②GABA和π亚基蛋白在妊娠第1日、第4日小鼠子宫内膜均有表达,第4日时在腔上皮的表达降低,而在基质细胞的表达升高;③第1日时,π亚基蛋白在子宫肌层有表达,但第4日时却不再表达。结论:①GABA和π亚基可能参与调节小鼠植入前期子宫内膜的容受性改变;②π亚基可能还参与植入前期子宫肌肉收缩,调节胚胎在子宫的空间分布。 展开更多
关键词 γ-氨基丁酸(GABA) π亚基 胚胎植入 子宫
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