期刊文献+
共找到61篇文章
< 1 2 4 >
每页显示 20 50 100
γ-Aminobutyric acid alleviates litchi thaumatin-like protein-induced inflammation and reduces gut microbial translocation
1
作者 Yao Wang Dongwei Wang +6 位作者 Kai Wang Min Zhao Cizhou Li Yu Wang Xuwei Liu Lei Zhao Zhuoyan Hu 《Food Science and Human Wellness》 SCIE CAS CSCD 2024年第5期3043-3053,共11页
Previous research reported litchi thaumatin-like protein(LcTLP)could lead to inflammation,which is a factor causing the adverse reactions after excessive intake of litchi.As a main amino acid in litchi pulp,γ-aminobu... Previous research reported litchi thaumatin-like protein(LcTLP)could lead to inflammation,which is a factor causing the adverse reactions after excessive intake of litchi.As a main amino acid in litchi pulp,γ-aminobutyric acid(GABA)was found with anti-inflammatory effect.Therefore,this study aimed to investigate the effects of GABA on LcTLP-induced inflammation through RAW264.7 macrophages and C57BL mice models.In vitro study showed GABA could effectively regulate the level of inflammatory cytokines(interleukin(IL)-1β,IL-6,IL-10,and prostaglandin E2)and Ca2+in cells,and inhibit the phosphorylation of p65,IκB,p38,c-Jun N-terminal kinase(JNK)and extracellular signal-regulated kinase(ERK).These results indicate GABA alleviated inflammation through nuclear factor-κB and mitogen-activated protein kinase pathway signaling pathways.In vivo experiment was performed to verify the anti-inflammatory effect of GABA,and the results demonstrated that GABA reduced the inflammation and oxidative stress in the liver of LcTLP-treated mice,as it down-regulated the pro-inflammatory cytokines,malondialdehyde,aspartate transferase,and alanine transaminase.The relative expression of phosphorylated p38,JNK and ERK in mice liver with GABA treatment were reduced to 65%,39%and 80%of the control group,respectively.Furthermore,GABA treatment enriched probiotic bacteria and decreased pathogenic bacteria in mice gut,which reveals GABA could effectively reduce the translocation of gut microbiota. 展开更多
关键词 ANTI-INFLAMMATION Gut microbiota Litchi thaumatin-like protein Mitogen-activated protein kinase pathway γ-aminobutyric acid
下载PDF
Neurosyphilis complicated by anti-γ-aminobutyric acid-B receptor encephalitis: A case report
2
作者 Ya-Xiu Fang Xiao-Ming Zhou +7 位作者 Dong Zheng Guang-Hui Liu Peng-Bo Gao Xiao-Zhen Huang Zhi-Cheng Chen Hui Zhang Lin Chen Ya-Fang Hu 《World Journal of Clinical Cases》 SCIE 2024年第11期1960-1966,共7页
BACKGROUND Syphilis is an infectious disease caused by Treponema pallidum that can invade the central nervous system,causing encephalitis.Few cases of anti-N-methyl-Daspartate receptor autoimmune encephalitis(AE)secon... BACKGROUND Syphilis is an infectious disease caused by Treponema pallidum that can invade the central nervous system,causing encephalitis.Few cases of anti-N-methyl-Daspartate receptor autoimmune encephalitis(AE)secondary to neurosyphilis have been reported.We report a neurosyphilis patient with anti-γ-aminobutyric acid-B receptor(GABABR)AE.CASE SUMMARY A young man in his 30s who presented with acute epileptic status was admitted to a local hospital.He was diagnosed with neurosyphilis,according to serum and cerebrospinal fluid(CSF)tests for syphilis.After 14 d of antiepileptic treatment and anti-Treponema pallidum therapy with penicillin,epilepsy was controlled but serious cognitive impairment,behavioral,and serious psychiatric symptoms were observed.He was then transferred to our hospital.The Mini-Mental State Examination(MMSE)crude test results showed only 2 points.Cranial magnetic resonance imaging revealed significant cerebral atrophy and multiple fluidattenuated inversion recovery high signals in the white matter surrounding both lateral ventricles,left amygdala and bilateral thalami.Anti-GABABR antibodies were discovered in CSF(1:3.2)and serum(1:100).The patient was diagnosed with neurosyphilis complicated by anti-GABABR AE,and received methylprednisolone and penicillin.Following treatment,his mental symptoms were alleviated.Cognitive impairment was significantly improved,with a MMSE of 8 points.Serum anti-GABABR antibody titer decreased to 1:32.The patient received methylprednisolone and penicillin after discharge.Three months later,the patient’s condition was stable,but the serum anti-GABABR antibody titer was 1:100.CONCLUSION This patient with neurosyphilis combined with anti-GABABR encephalitis benefited from immunotherapy. 展开更多
关键词 Anti-γ-aminobutyric acid-B receptor GABABR NEUROSYPHILIS Tissue-based assay Magnetic resonance imaging Mini-mental state examination Case report
下载PDF
Environmental cues associated with morphine modulate release of glutamate and γ-aminobutyric acid in ventral subiculum 被引量:2
3
作者 康林 戴正泽 +1 位作者 李浩洪 马兰 《Neuroscience Bulletin》 SCIE CAS CSCD 2006年第5期255-260,共6页
Objective To investigate whether environmental cues associated with different properties of morphine could regulate the extracellular levels of glutamate and y-aminobutyric acid (GABA) in the hippocampal ventral sub... Objective To investigate whether environmental cues associated with different properties of morphine could regulate the extracellular levels of glutamate and y-aminobutyric acid (GABA) in the hippocampal ventral subiculum, which play a critical role in the reinstatement of drug-seeking behavior induced by environmental cues. Methods Conditioning place preference (CPP) and conditioning place aversion (CPA) models were used to establish environment associated with rewarding and aversive properties of morphine respectively. Microdialysis and high performance liquid chromatography were used to measure the extracelluar level of glutamate and GABA in the ventral subiculum under these environmental cues. Results Exposure to the environmental cues associated with rewarding properties of morphine resulted in a decrease (approximately 11%) of extracellular level of GABA in ventral subiculum, and exposure to the environmental cues associated with aversive properties of morphine resulted in an increase (approximately 230%) of extracellular level of glutamate in ventral subiculum. Conclusion Environmental cues associated with different properties of morphine modulate the release of distinct neurotransmitters in the hippocampal ventral subiculum possibly through different neural circuit. 展开更多
关键词 conditioning place preference conditioning place aversion ventral subiculum MICRODIALYSIS γ-aminobutyric acid GLUTAMATE
下载PDF
Modulation of γ-aminobutyric acid on painful sense in central nervous system of morphine-dependent rats 被引量:1
4
作者 许艳 徐满英 李霞 《Neuroscience Bulletin》 SCIE CAS CSCD 2008年第5期278-282,共5页
Objective To observe the effects of y-aminobutyric acid (GABA) on the electric activities of pain-excited neurons (PEN) in nucleus accumbens (NAc) in central nervous system (CNS) of morphine-dependent rats. Me... Objective To observe the effects of y-aminobutyric acid (GABA) on the electric activities of pain-excited neurons (PEN) in nucleus accumbens (NAc) in central nervous system (CNS) of morphine-dependent rats. Methods After GABA or the GABAA-receptor antagonist, bicuculline (Bic), was injected into cerebral ventricles or NAc, right sciatic nerve was stimulated by electrical pulses, which was considered as traumatic pain stimulation. Extracellular recordings methods were used to record the electric activities of PEN in NAc. Results When GABA was injected into intracerebroventricle (ICV) as well as NAc, it could decrease the pain-evoked discharge frequency and prolong the latency of PEN. Bic could interdict the above effects of GABA on the electric activities of PEN. Conclusion Exogenous GABA might have an inhibitory effect on the central pain adjustment. Furthermore, GABA and GABAA receptor participate and mediate the traumatic information transmission process in CNS. 展开更多
关键词 γ-aminobutyric acid pain-excited neurons nucleus accumbens bicueulline morphine-dependent rats
下载PDF
Overexpression of γ-aminobutyric acid transporter subtype I leads to susceptibility to Kainic acid-induced seizure in transgenic mice 被引量:10
5
作者 MaYH HuJH 《Cell Research》 SCIE CAS CSCD 2001年第1期61-67,共7页
γ-aminobutyric acid (GABA) is the principal inhibitory neurotransmitter, and the GABAergic synaptic transmission is normally terminated by the rapid uptake through GABA transporters. With transgenic mice ubiquitously... γ-aminobutyric acid (GABA) is the principal inhibitory neurotransmitter, and the GABAergic synaptic transmission is normally terminated by the rapid uptake through GABA transporters. With transgenic mice ubiquitously overexpressing GABA transporter subtype I (GAT1), the present study explored the pathophysiological role of GAT1 in epileptogenesis. Though displaying no spontaneous seizure activity, these mice exhibit altered electroencephalographic patterns and increased susceptibility to seizure induced by kainic acid. In addition, the GABAA receptor and glutamate transporters are up-regulated in transgenic mice, which perhaps reflects a compensatory or corrective change to the elevated level of GAT1. These preliminary findings support the hypothesis that excitatory and inhibitory neurotransmission, and seizure susceptibility can be altered by neurotransmitter transporters. 展开更多
关键词 γ-aminobutyric acid transporter SEIZURE SUSCEPTIBILITY kainic acid ELECTROENCEPHALOGRAPHY transgenic mice
下载PDF
γ-Aminobutyric acid transporter (GAT1) overexpression in mouse affects the testicular morphology 被引量:5
6
作者 MA YING HUA JIA HUA HU +3 位作者 XIAO GANG ZHOU ZHEN TONG MEI JIAN FEI LI HE GUO(Shanghai Institute of Cell Biology, Chinese Academy of Science, Shanghai 200031, China Shanghai Institute of Physiology, Chinese Academy of Science, Shanghai 200031, China) 《Cell Research》 SCIE CAS CSCD 2000年第1期59-69,共11页
γ-Aminobutyric acid and GABAergic receptors were previously reported to be distributed in reproductive systems besides CNS and predicted to participate in the modulation of testicular function. γ-Aminobutyric acid t... γ-Aminobutyric acid and GABAergic receptors were previously reported to be distributed in reproductive systems besides CNS and predicted to participate in the modulation of testicular function. γ-Aminobutyric acid transporter was implicated to be involved in this process. However, the potential role of γ-aminobutyric transporter in testis has not been explored. In this study, we investigated the existence of mouse γ-aminobutyric acid transporter subtype I (mGAT1) in testis. Wild-type and transgenic mice, which overexpressing mGAT1 in a variety of tissues, especially in testis, were primarily studied to approach the profile of mGAT1 in testis. Mice with overexpressed mGAT1 develop normally but with reduced mass and size of testis as compared with wild-type. Testicular morphology of transgenic mice exhibited overt abnormalities including focal damage of the spermatogenic epithelium accompanied by capillaries proliferation and increased diameter of seminiferous tubules lumen. Reduced number of spermatids was also found in some seminiferous tubules. Our results clearly demonstrate the presence of GAT1 in mouse testis and imply that GAT1 is possibly involved in testicular function. 展开更多
关键词 γ-aminobutyric acid transporter testicular morphology mouse.
下载PDF
Effect of the pineal gland on 5-hydroxytryptamine and γ-aminobutyric acid secretion in the hippocampus of male rats during the summer and winter 被引量:4
7
作者 Ting Li Hailu Wang +3 位作者 Hewei Zhang Leilei Liu Peipei Li Shuran Ma 《Journal of Traditional Chinese Medical Sciences》 2020年第3期283-290,共8页
Objective:The present study aimed to investigate the effect of seasonal variation on neurotransmitter release in the hippocampus of normal rats and rats with pineal excision.Methods:Two time points,the summer and wint... Objective:The present study aimed to investigate the effect of seasonal variation on neurotransmitter release in the hippocampus of normal rats and rats with pineal excision.Methods:Two time points,the summer and winter solstice,which are the longest and shortest days of the year,respectively,were selected.Male Spraguee Dawley rats that underwent a sham operation without pineal excision were included as a control group.The concentrations of 5-hydroxytryptamine(5-HT)andγ-aminobutyric acid(GABA)were determined by radioimmunoassays and enzyme-linked immunosorbent assays,respectively.Results:In the winter,the 5-HT and GABA levels in normal rats exhibited a significant difference compared with those in the operation group(P<.01).A difference was also noted in GABA levels between the normal group and the sham operation group(P<.05).The concentrations of 5-HT and GABA in the hippocampal tissues of the normal group exhibited a seasonal rhythm consisting of elevation during the summer and reduction during the winter(P<.01),while the GABA levels in the sham operation group exhibited a significant difference,with elevation during the summer and reduction during the winter(P<.01).In the operation group,GABA showed the same trend(P<.01).Conclusion:The seasonal rhythm of neurotransmitter secretion by the hippocampus(5-HT and GABA)consisted of elevation during the summer and reduction during the winter.During the winter,the pineal gland exhibited a reverse regulatory effect on the secretion of 5-HT and GABA in the hippocampus,and it exhibited seasonal selectivity with regard to the regulation of 5-HT. 展开更多
关键词 MELATONIN 5-HYDROXYTRYPTAMINE γ-aminobutyric acid PHOTOPERIOD Rats Seasonal rhythm Pineal excision
下载PDF
On-line near-infrared spectroscopy optimizing and monitoring biotransformation process of γ-aminobutyric acid 被引量:2
8
作者 Guoyu Ding Yuanyuan Hou +3 位作者 Jiamin Peng Yunbing Shen Min Jiang Gang Bai 《Journal of Pharmaceutical Analysis》 SCIE CAS 2016年第3期171-178,共8页
Near-infrared spectroscopy (NIRS) with its fast and nondestructive advantages can be qualified for the real-time quantitative analysis. This paper demonstrates that NIRS combined with partial least squares (PLS) r... Near-infrared spectroscopy (NIRS) with its fast and nondestructive advantages can be qualified for the real-time quantitative analysis. This paper demonstrates that NIRS combined with partial least squares (PLS) regression can be used as a rapid analytical method to simultaneously quantify L-glutamic acid (L- GIu) and γ-aminobutyric acid (GABA) in a biotransformation process and to guide the optimization of production conditions when the merits of NIRS are combined with response surface methodology. The high performance liquid chromatography (HPLC) reference analysis was performed by the o-phthaldialdehyde pre-column derivatization. NIRS measurements of two batches of 141 samples were firstly analyzed by PLS with several spectral pre-processing methods. Compared with those of the HPLC reference analysis, the resulting determination coefficients (R2), root mean square error of prediction (RMSEP) and residual predictive deviation (RPD) of the external validation for the L-GIu concentration were 99.5%, 1.62 g/L, and 11.3, respectively. For the GABA concentration, R2, RMSEP, and RPD were 99.8%, 4.00 g/L, and 16.4, respectively. This NIRS model was then used to optimize the biotransformation process through a Box- Behnken experimental design. Under the optimal conditions without pH adjustment, 200 gjL L-GIu could be catalyzed by 7148 U/L glutamate decarboxylase (GAD) to GABA, reaching 99% conversion at the fifth hour. NIRS analysis provided timely information on the conversion from L-GIu to GABA. The results suggest that the NIRS model can not only be used for the routine profiling of enzymatic conversion, providing a simple and effective method of monitoring the biotransformation process of GABA, but also be considered to be an optimal tool to guide the optimization of production conditions. 展开更多
关键词 Near-intrared spectroscopy L-glutamic acid γ-aminobutyric acid Box-Behnken design Glutamate decarboxylase
下载PDF
Effect of Propofol on Glutamate and γ-aminobutyric Acid Release from Rat Hippocampal Synaptosomes 被引量:2
9
作者 尚游 姚尚龙 +2 位作者 曾因明 刘红亮 曹君利 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2005年第6期700-702,共3页
To investigate the effect of propofol on the release of glutamate and γ-aminobutyric acid (GABA) from rat hippocampal synatosomes, synaptosomes was made from hippocampus and incubated with artificial cerebrospinal ... To investigate the effect of propofol on the release of glutamate and γ-aminobutyric acid (GABA) from rat hippocampal synatosomes, synaptosomes was made from hippocampus and incubated with artificial cerebrospinal fluid (aCSF). With the experiment of Ca^2+-dependent release of glutamate and GABA, dihydrokainic acid (DHK) and nipectic acid were added into aCSF. For the observation of Ca^2+-independent release of glutamate and GABA, no DHK, nipectic acid and Ca^2+ were added from aCSF. The release of glutamate and GABA were evoked by 20 μmol/L veratridine or 30 mmol/L KCh The concentration of glutamate and GABA in aCSF was measured by using high-performance liquid chromatography (HPLC). 30, 100 arid 300 μmol/L propofol significantly inhibited veratridine-evoked Ca^2+-dependent release of glutamate and GABA (P〈0. 01 or P〈0. 05), However, propofol showed no effect on elevated KCl-evoked Ca^2+-dependent release of glutamate and GABA (P〉0, 05), Veratridine or elevated KCI evoked Ca^2+-independent release of glutamate and GABA was not affected significantly by propofol (P〉0.05). Propofol could inhibit Ca^2+- dependent release of glutamate and GABA, However, it has no effect on the Ca^2+-independent release of glutamate and GABA, 展开更多
关键词 PROPOFOL HIPPOCAMPUS SYNAPTOSOME GLUTAMATE γ-aminobutyric acid
下载PDF
Incorporation ofγ-aminobutyric acid and cesium cations to formamidinium lead halide perovskites for highly efficient solar cells 被引量:1
10
作者 Yanqiang Hu Yansu Shan +10 位作者 Zhaolei Yu Haojie Sui Ting Qiu Shufang Zhang Wei Ruan Qinfeng Xu Mengmeng Jiao Dehua Wang Yunyi Wu Chuanlu Yang Feng Xu 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2022年第1期561-567,I0015,共8页
The stability issue has become one of the main challenges for the commercialization of perovskite solar cells(PSCs).Formamidinium(FA)-based perovskites have shown great promise owing to their improved thermal and mois... The stability issue has become one of the main challenges for the commercialization of perovskite solar cells(PSCs).Formamidinium(FA)-based perovskites have shown great promise owing to their improved thermal and moisture stability.However,these perovskites are suffering from phase transition and separation.Here,a method of incorporating of γ-aminobutyric acid(GABA) and cesium cations into FAPbl_(3) is developed to improve the phase stability.It is demonstrated that the crystallinity of α-FAPbl_(3) phase is greatly improved and the phase transition temperature is significantly dropped.The resultant solar cell therefore obtains a champion power conversion efficiency(PCE) of 23.71%,which is one of the highest efficiencies for methylammonium-free PSCs.Furthermore,it shows an impressively enhanced stability under illumination,exhibiting the great potential of FA-based perovskites for efficient and stable solar cells. 展开更多
关键词 Perovskite solar cells γ-aminobutyric acid Methylammonium-free Stability
下载PDF
Transgenic mice overexpressingγ-aminobutyric acid transporter subtypeⅠ develop obesity 被引量:1
11
作者 MA YING HUA JIA HUA HU +5 位作者 XIAO GANG ZHOU RUO WANG ZENG ZHEN TONG MEI JIAN FEI LI HE GUO Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science, Shanghai 200031, China Shanghai Institute of 《Cell Research》 SCIE CAS CSCD 2000年第4期303-310,共8页
Transgenic mice ubiquitously overexpressing murine γ aminobutyric acid transporter subtype Ⅰ were created. Unexpectedly, these mice markedly exhibited heritable obesity, which features significantly increased body w... Transgenic mice ubiquitously overexpressing murine γ aminobutyric acid transporter subtype Ⅰ were created. Unexpectedly, these mice markedly exhibited heritable obesity, which features significantly increased body weight and fat deposition. Behavioral examination revealed that transgeinc mice have slightly reduced spontaneous locomotive capacity and altered feeding pattern. Tills preliminary finding indicates that the inappropriate level of γ-aminobutyric acid transporters may be directly or indirectly involved in the pathogenic mechanism underlying certain types of obesity. 展开更多
关键词 γ-aminobutyric acid transporter subtype transgenic mouse obesit€
下载PDF
Γ-Aminobutyric acid promotes methionine-choline deficient diet-induced nonalcoholic steatohepatitis 被引量:1
12
作者 Yoon Seok Roh Ara Cho +6 位作者 Zixiong Zhou Hyuneui Jeong Jeong-Eun Park Youn-Soo Cha Suk-Heung Oh Chae-Woong Lim Bumseok Kim 《The Journal of Biomedical Research》 CAS CSCD 2017年第1期65-73,共9页
Nonalcoholic steatohepatitis(NASH) is one of the most common liver diseases and a major cause of liver fibrosis worldwide.r-Aminobutyric acid(GABA) is one of the most abundant inhibitory neurotransmitters in the c... Nonalcoholic steatohepatitis(NASH) is one of the most common liver diseases and a major cause of liver fibrosis worldwide.r-Aminobutyric acid(GABA) is one of the most abundant inhibitory neurotransmitters in the central nervous system.Recently,it has been reported that GABAergic signaling pathways are found in various non-neuronal tissues including the immune system and play a functional role.In the present study,we investigated whether administration of GABA has effects on NASH through its immunomodulatory effects.To test this hypothesis,C57BL/6 mice were fed a methionine-choline-deficient(MCD) diet for 8 weeks.After four weeks into MCD feeding,mice were provided with plain water(control) or water containing 2 mg/mL of GABA for the subsequent 4 weeks.Using this MCD diet-induced NASH model,we found that mice receiving GABA showed more severe steatohepatitis and liver fibrosis than control mice.This increased liver damage was confirmed by higher levels of serum alanine transaminase(ALT) and aspartate aminotransferase(AST) compared to the control group.In accordance with increased liver steatohepatitis,NASH-related and inflammatory gene expression(collagen al,tissue inhibitor of metalloproteinase-1,TNF-α) in the liver was markedly increased in GABA-treated mice.Furthermore,GABA directly enhanced production of inflammatory cytokines including IL-6 and TNF-α in LPS activated RAW macrophage cells and increased TIB-73 hepatocyte death.Such effects were abolished when GABA was treated with bicuculline,a competitive antagonist of GABA receptors.These results suggest that oral administration of GABA may be involved in changes of the liver immune milieu and conferred detrimental effects on NASH progression. 展开更多
关键词 γ-aminobutyric acid nonalcoholic steatohepatitis methionine-choline-deficient diet mice GABA
下载PDF
Connections between 5-HT-containing terminals and 5-HT_(2A) recep tor and γ-aminobutyric acid or glycine co-existed neurons in the rat medullary dorsal horn
13
作者 李辉 李云庆 《Journal of Medical Colleges of PLA(China)》 CAS 2001年第3期190-192,199,共4页
Objective: To investigatetheconnectionsbetweenserotonin(5-HT)-containingterminalsand5-HT 2A receptor(5-HT 2A R)/γ-aminobutyricacid(GABA)or5-HT 2A R/glycineco-existedneuronsin theratmedullarydorsalhorn(MDH).Meth ods:I... Objective: To investigatetheconnectionsbetweenserotonin(5-HT)-containingterminalsand5-HT 2A receptor(5-HT 2A R)/γ-aminobutyricacid(GABA)or5-HT 2A R/glycineco-existedneuronsin theratmedullarydorsalhorn(MDH).Meth ods:Immunofluorescencehistochemicaltriple-stainingfor5-HT,5-HT 2A R,GABAor glycine.Results:5-HT-im-munoreactivefibersandterminalswerechieflylocatedinthesuperficiallaminae(laminaeⅠandⅡ)of theMDH.Neurons exhibiting5-HT 2A R-,GABA-or glycine-immunoreactivitiesweremainlyobservedin the superficiallaminae.Some5-HT 2A R-immunopositiveneuronsalsoexhibitedGABA-or glycine-immunoreactivities.5-HT-containingterminalsmade closecontactswith5-HT 2A R/GABAor5-HT 2A R/glycineco-existedneurons.Conclusion:5-HT 2A R/GABAor5-HT 2A R/glycineco-existinsomeof theneuronsinthesuperficiallaminaeof theMDH.5-HT-immunoreactiveterminalsformclose connectionswith5-HT 2A R/GABAor5-HT 2A R/glycineco-existedneurons. 展开更多
关键词 serotonin γ-aminobutyric acid GLYCINE ANTINOCICEPTION DESCENDING inhibitory system MEDULLARY dorsal horn RAT
下载PDF
Synthesis and Anticonvulsant Activity of γ-Aminobutyric Acid Derivatives
14
作者 Mu YUAN Yu Guo ZHONG +1 位作者 Qi Jie PANG(Department of Pharmacy, Guiyang Medical College, Gujyang 550004 School of Pharmacy, West China University of Medical Sciences, Chengdu 610044) 《Chinese Chemical Letters》 SCIE CAS CSCD 1998年第4期369-371,共3页
Under physiological conditions, γ-aminobutyric acid poorly crosses the blood-brain barrier. It is likely that a non-toxic derivative of γ-aminobutyric acid which enters the brain easily will have useful anticonvulsa... Under physiological conditions, γ-aminobutyric acid poorly crosses the blood-brain barrier. It is likely that a non-toxic derivative of γ-aminobutyric acid which enters the brain easily will have useful anticonvulsant activity. 16 derivatives of γ-aminobutyric acid with an imine link to a lipophilic carrier were prepared and tested for anticonvulsant activity; six compounds show anticonvulsant activity. 展开更多
关键词 γ-aminobutyric acid SYNTHESIS anticonvulsant activity
下载PDF
Effects ofγ-aminobutyric Acid on Nitrogen Metabolism in Roots and Leaves of Cold-stressed Rice(Oryza sativa L.)During Early Vegetative Growth
15
作者 Jia Yan Gong Weibin +9 位作者 Ma Huimiao Liu Ge Zhang Can Liu Aixin Han Yiming Dang Yuxiang Bai Xu Wang Haixing Wu Yulong Xin Junying 《Journal of Northeast Agricultural University(English Edition)》 CAS 2024年第3期1-19,共19页
Cold stress adversely affects rice growth,particularly at the early vegetative growth stage.In higher plants,nitrogen metabolism plays a central role in amino acid metabolism,plant defense mechanisms and productivity.... Cold stress adversely affects rice growth,particularly at the early vegetative growth stage.In higher plants,nitrogen metabolism plays a central role in amino acid metabolism,plant defense mechanisms and productivity.This report investigated the effects of cold stress and supplementalγ-aminobutyric acid(GABA)under cold stress on nitrogen metabolism in rice seedlings.Cold stress resulted in a greater increase in the transformation to NH_(4)^(+)by nitrate reductase(NR)in roots,it further resulted in lower levels of NO_(3)^(-)content in roots,weakened glutamine glutamate(GOGAT/GS)pathway and elevated glutamate dehydrogenase(GDH)pathway of rice seedlings.Whereas,compared with cold stress,supplementation of GABA(2.5 mmol·L^(-1))could increase relative water content(79.43%)and biomass(34.15%)of rice seedlings.GABA could act as an amplifier of stress signal conduction/transduction to increase NR activity and promote NO_(3)^(-)assimilation in leaves.In addition,GABA elicited the Ca^(2+)signaling pathway which could promote the GDH pathway and GABA shunt,increase the activities of GS and GDH,and the expression of OsGAD2 and OsGDH family.The GABA might increase the ratio of the Glu family and avoid NH4+toxicity in order to raise the concentration of organic compounds and alleviate the harmful consequences of cold stress.Based on these observations,this study proposed that GABA mediated cold tolerance in rice seedlings by activating Ca^(2+)burst and subsequent crosstalk among Ca^(2+)signaling,GDH pathway and GABA shunt. 展开更多
关键词 cold stress γ-aminobutyric acid rice nitrogen metabolism
下载PDF
High-level production of𝛾γ-aminobutyric acid via efficient co-expression of the key genes of glutamate decarboxylase system in Escherichia coli
16
作者 Lili Yao Changjiang Lyu +5 位作者 Yuting Wang Sheng Hu Weirui Zhao Hongwei Cao Jun Huang Lehe Mei 《Engineering Microbiology》 2023年第2期65-71,共7页
Biosynthesis of the functional factor𝛾γ-aminobutyric acid(GABA)in bacteria involves two key proteins an intra-cellular glutamate decarboxylase(GadB)and a membrane-bound antiporter(GadC).Efficient co-expressio... Biosynthesis of the functional factor𝛾γ-aminobutyric acid(GABA)in bacteria involves two key proteins an intra-cellular glutamate decarboxylase(GadB)and a membrane-bound antiporter(GadC).Efficient co-expression of suitable GadB and GadC candidates is crucial for improving GABA productivity.In this study,gadBΔC11 of Lacti-plantibacillus plantarum and gadCΔC41 of Escherichia coli were inserted into the designed double promoter(P T7lac and P BAD)expression system.Then,E.coli Lemo21(DE3)was chosen as the host to minimize the toxic effects of GadCΔC41 overexpression.Furthermore,a green and high-efficiency GABA synthesis system using dormant engineered Lemo21(DE3)cells as biocatalysts was developed.The total GABA yield reached 829.08 g/L with a 98.7%conversion ratio within 13 h,when engineered E.coli Lemo21(DE3)cells were concentrated to an OD 600 of 20 and reused for three cycles in a 3 M L-glutamate solution at 37℃,which represented the highest GABA productivity ever reported.Overall,expanding the active pH ranges of GadB and GadC toward physiological pH and employing a tunable expression host for membrane-bound GadC production is a promising strategy for high-level GABA biosynthesis in E.coli. 展开更多
关键词 Glutamate decarboxylase ANTIPORTER γ-aminobutyric acid E.COLI Whole-cell biocatalysts
原文传递
Expression of gamma-aminobutyric acid type A receptor α_2 subunit in the dorsal root ganglion of rats with sciatic nerve injury 被引量:1
17
作者 Yu Lian Yang Wang +5 位作者 Ketao Ma Lei Zhao Zhongshuang Zhang Yuanyuan Shang Junqiang Si Li Li 《Neural Regeneration Research》 SCIE CAS CSCD 2012年第32期2492-2499,共8页
The γ-aminobutyric acid neurotransmitter in the spinal cord dorsal horn plays an important role in pain modulation through primary afferent-mediated presynaptic inhibition. The weakening of γ-aminobutyric acid-media... The γ-aminobutyric acid neurotransmitter in the spinal cord dorsal horn plays an important role in pain modulation through primary afferent-mediated presynaptic inhibition. The weakening of γ-aminobutyric acid-mediated presynaptic inhibition may be an important cause of neuropathic pain. γ-aminobutyric acid-mediated presynaptic inhibition is related to the current strength of γ-aminobutyric acid A receptor activation. In view of this, the whole-cell patch-clamp technique was used here to record the change in muscimol activated current of dorsal root ganglion neurons in a chronic constriction injury model. Results found that damage in rat dorsal root ganglion neurons following application of muscimol caused concentration-dependent activation of current, and compared with the sham group, its current strength and γ-aminobutyric acid A receptor protein expression decreased. Immunofluorescence revealed that γ-aminobutyric acid type A receptor α2 subunit protein expression decreased and was most obvious at 12 and 15 days after modeling. Our experimental findings confirmed that the y-aminobutyric acid type A receptor α2 subunit in the chronic constriction injury model rat dorsal root ganglion was downregulated, which may be one of the reasons for the reduction of injury in dorsal root ganglion neurons following muscimol-activated currents. 展开更多
关键词 γ-aminobutyric acid γ-aminobutyric acid type A receptor α2 subunit neuropathic pain dorsal root ganglion whole-cell patch clamp IMMUNOFLUORESCENCE primary afferent depolarization paw withdrawal latency MUSCIMOL
下载PDF
Effects of gamma-aminobutyric acid receptors on muscarinic receptor-mediated free calcium ion levels in the facial nucleus following facial nerve injury
18
作者 Guangfeng Jiang Dawei Sun +4 位作者 Rui Zhou Fugao Zhu Yanqing Wang Xiuming Wan Banghua Liu 《Neural Regeneration Research》 SCIE CAS CSCD 2011年第11期855-859,共5页
Muscarinic receptors and nicotine receptors can increase free calcium ion levels in the facial nucleus via different channels following facial nerve injury. In addition, γ-aminobutyric acid A (GABAA) receptors have... Muscarinic receptors and nicotine receptors can increase free calcium ion levels in the facial nucleus via different channels following facial nerve injury. In addition, γ-aminobutyric acid A (GABAA) receptors have been shown to negatively regulate free calcium ion levels in the facial nucleus by inhibiting nicotine receptors. The present study investigated the influence of GABAA, γ-aminobutyric acid B (GABAB) and C (GABAc) receptors on muscarinic receptors in rats with facial nerve injury by confocal laser microscopy. GABAA and GABAB receptors exhibited significant dose-dependent inhibitory effects on increased muscarinic receptor-mediated free calcium ion levels following facial nerve injury. Results showed that GABAA and GABAB receptors negatively regulate muscarinic receptor effects and interplay with cholinergic receptors to regulate free calcium ion levels for facial neural regeneration. 展开更多
关键词 injury of facial nerve γ-aminobutyric acid A receptor γ-aminobutyric acid B receptor muscarinic receptor facial nucleus calcium ion peripheral nerve injury neural regeneration
下载PDF
Relationship of nocturnal concentrations of melatonin, gamma-aminobutyric acid and total antioxidants in peripheral blood with insomnia after stroke: study protocol for a prospective non-randomized controlled trial 被引量:47
19
作者 Wei Zhang Fang Li Tong Zhang 《Neural Regeneration Research》 SCIE CAS CSCD 2017年第8期1299-1307,共9页
Melatonin and gamma-aminobutyric acid(GABA) have been shown to regulate sleep. The nocturnal concentrations of melatonin, GABA and total antioxidants may relate to insomnia in stroke patients. In this prospective si... Melatonin and gamma-aminobutyric acid(GABA) have been shown to regulate sleep. The nocturnal concentrations of melatonin, GABA and total antioxidants may relate to insomnia in stroke patients. In this prospective single-center non-randomized controlled clinical trial performed in the China Rehabilitation Research Center, we analyzed the relationship of nocturnal concentrations of melatonin, GABA and total antioxidants with insomnia after stroke. Patients during rehabilitation of stroke were recruited and assigned to the insomnia group or non-insomnia group. Simultaneously, persons without stroke or insomnia served as normal controls. Each group contained 25 cases. The primary outcome was nocturnal concentrations of melatonin, GABA and total antioxidants in peripheral blood. The secondary outcomes were Pittsburgh Sleep Quality Index, Insomnia Severity Index, Epworth Sleepiness Scale, Fatigue Severity Scale, Morningness-Eveningness Questionnaire(Chinese version), and National Institute of Health Stroke Scale. The relationship of nocturnal concentrations of melatonin, GABA and total antioxidants with insomnia after stroke was analyzed and showed that they were lower in the insomnia group than in the non-insomnia group. The severity of stroke was higher in the insomnia group than in the non-insomnia group. Correlation analysis demonstrated that the nocturnal concentrations of melatonin and GABA were associated with insomnia after stroke. This trial was registered at Clinical Trials.gov, identifier: NCT03202121. 展开更多
关键词 nerve regeneration STROKE INSOMNIA MELATONIN γ-aminobutyric acid total antioxidants sleep-related scales National Institute ofHealth Stroke Scale neural regeneration
下载PDF
Activation of γ-aminobutyric Acid (A) Receptor Protects Hippocampus from Intense Exercise-induced Synapses Damage and Apoptosis in Rats 被引量:3
20
作者 Yi Ding Lan Xie Cun-Qing Chang Zhi-Min Chen Hua Ai 《Chinese Medical Journal》 SCIE CAS CSCD 2015年第17期2330-2339,共10页
Background: Our previous study has confirmed that one bout of exhaustion (Ex) can cause hippocampus neurocyte damage, excessive apoptosis, and dysfunction, its initial reason is intracellular calcium overload in hi... Background: Our previous study has confirmed that one bout of exhaustion (Ex) can cause hippocampus neurocyte damage, excessive apoptosis, and dysfunction, its initial reason is intracellular calcium overload in hippocampus triggered by N-methyl-D-aspartic acid receptor (NMDAR) over-activation. NMDAR activation can be suppressed by y-aminobutyric acid (A) receptor (GABAAR). Whether GABAAR can prevent intense exercise-induced hippocampus apoptosis, damage, or dysfunction will be studied in this study. Methods: According to dose test, rats were randomly divided into control (Con), Ex, muscimol (MUS, 0.1 mg/kg) and bicuculline (BIC, 0.5 mg/kg) groups, then all rats underwent once swimming Ex except ones in Con group only underwent training, lntracellular free calcium concentration ([Ca2+]i) was measured by Fura-2-acetoxymethyl ester; glial fibrillary acidic protein (GFAP) and synaptophysin (SYP) immunofluorescence were also performed; apoptosis were displayed by dUTP nick end labeling (TUNEL) stain; endoplasmic reticulum stress-induced apoptosis pathway was detected by Western blotting analysis; Morris water maze was used to detect learning ability and spatial memory. Results: The appropriate dose was 0.1 mg/kg for MUS and 0.5 mg/kg for BIC. Ex group showed significantly increased [Ca^2+]i and astrogliosis; TUNEL positive cells and levels of GFAP, B cell lymphoma-2 (Bcl-2) associated X protein (Bax), caspase-3, caspase-12 cleavage, CCAAT/enhancer binding protein homologous protein (CHOP), and p-Jun amino-terminal kinase (p-JNK) in Ex group also raised significantly compared to Con group, while SYP, synapse plasticity, and Bcl-2 levels in Ex group were significantly lower than those in Con group. These indexes were back to normal in MUS group. BIC group had the highest levels of [Ca^2+]i, astrogliosis, TUNEL positive cell, GFAP, Bax, caspase-3, caspase-12 cleavage, CHOP, and p-JNK, it also gained the lowest SYP, synapse plasticity, and Bcl-2 levels among all groups. Water maze test showed that Ex group had longer escape latency (EL) and less quadrant dwell time than Con group; all indexes between MUS and Con groups had no significant differences; BIC had the longest EL and least quadrant dwell time among all groups. Conclusions: Activation of GABAAR could prevent intense exercise-induced synapses damage, excessive apoptosis, and dysfunction of hippocampus. 展开更多
关键词 Endoplasmic Reticulum Stress-induced Apoptosis γ-aminobutyric Acid (A) Receptor HIPPOCAMPUS Intense Exercise Synapse Plasticity
原文传递
上一页 1 2 4 下一页 到第
使用帮助 返回顶部