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三疣梭子蟹Na^+/H^+-exchanger基因克隆鉴定及在盐度胁迫下的表达分析 被引量:8
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作者 马金武 吕建建 +2 位作者 刘萍 高保全 李健 《水生生物学报》 CAS CSCD 北大核心 2016年第5期902-907,共6页
为研究Na^+/H^+-exchanger基因在三疣梭子蟹(Portunus trituberculatus)盐度胁迫过程中的功能作用,克隆了三疣梭子蟹Na^+/H^+-exchanger基因并进行表达分析。结果显示,Na^+/H^+-exchanger基因(Gen Bank:KU519329)全长4233 bp,5?和3?非... 为研究Na^+/H^+-exchanger基因在三疣梭子蟹(Portunus trituberculatus)盐度胁迫过程中的功能作用,克隆了三疣梭子蟹Na^+/H^+-exchanger基因并进行表达分析。结果显示,Na^+/H^+-exchanger基因(Gen Bank:KU519329)全长4233 bp,5?和3?非编码区(UTR)长分别为519和753 bp,开放阅读框(ORF)长2961 bp。编码986个氨基酸,预测蛋白质分子量和等电点分别为110.8 k D和7.42,具有信号肽和典型的Na^+/H^+-exchanger蛋白结构域,含12个跨膜α螺旋;三疣梭子蟹Na^+/H^+-exchanger基因与普通滨蟹(Carcinus maenas)同源性最高,达到87.2%,系统进化分析也显示该序列与普通滨蟹聚为一支;表达分析显示,三疣梭子蟹Na^+/H^+-exchanger基因在鳃中表达量最高;在低盐(盐度5、10和20)胁迫过程中,Na^+/H^+-exchanger基因在0—12h上调表达明显,在24—168h间表达量呈下降趋势;在高盐(盐度50)胁迫初期(0—12h),该基因表达量相对稳定,之后(24—168h)显著下调表达。研究表明低盐显著诱导Na^+/H^+-exchanger基因的高表达,推测三疣梭子蟹Na^+/H^+-exchanger基因在低盐环境下发挥重要的渗透调节功能。 展开更多
关键词 三统梭子蟹 Na^+/H^+-exchanger 基因克隆 盐度胁迫 表达分析
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拟穴青蟹Na^+/H^+-exchanger基因克隆及其在盐度胁迫下的表达分析 被引量:2
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作者 刁乐 张凤英 +7 位作者 宋炜 马春艳 王伟 谌微 马凌波 赵明 乔振国 蒋科技 《海洋渔业》 CSCD 北大核心 2020年第2期214-224,共11页
克隆了拟穴青蟹(Scylla paramamosain)Na^+/H^+-exchanger基因,其cDNA序列全长3624 bp,开放阅读框(ORF)长2886 bp,可编码961个氨基酸,预测蛋白质分子量和等电点分别为110.8 kDa和7.42,具有信号肽和典型的Na^+/H^+-exchanger蛋白结构域,... 克隆了拟穴青蟹(Scylla paramamosain)Na^+/H^+-exchanger基因,其cDNA序列全长3624 bp,开放阅读框(ORF)长2886 bp,可编码961个氨基酸,预测蛋白质分子量和等电点分别为110.8 kDa和7.42,具有信号肽和典型的Na^+/H^+-exchanger蛋白结构域,含12个跨膜α螺旋。拟穴青蟹Na^+/H^+-exchanger的氨基酸序列与三疣梭子蟹(Portunus trituberculatus)的一致度最高,达到84.9%;该基因在卵巢中表达量最高,其次是精巢、鳃等;该基因在受精卵时期表达水平最高,大眼幼体时期次之。拟穴青蟹大眼幼体在低盐(盐度7和17)胁迫过程中,Na^+/H^+-exchanger基因在20 min表达水平最高,之后基本恢复到正常表达水平;在高盐(盐度37)胁迫(20 min^2 h)期间,该基因表达水平先下降再逐渐上升。Na^+/H^+-exchanger基因在拟穴青蟹大眼幼体阶段盐度适应中发挥着重要作用,且低盐显著诱导Na^+/H^+-exchanger基因的高表达,推测拟穴青蟹Na^+/H^+-exchanger基因在低盐环境下发挥重要的渗透调节功能。 展开更多
关键词 拟穴青蟹 Na^+/H^+-exchanger 盐度胁迫 表达分析
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Preparation and Characterization of Antibacterial Zn^(2+)-Exchanged Montmorillonites 被引量:1
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作者 施庆珊 谭绍早 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2010年第5期725-729,共5页
Zn-montmorillonites(Zn-MMTs) as antibacterial compounds were prepared by an ion-exchange reaction. The reaction time, initial pH value and molar ratios of CEC influencing zinc content in Zn-MMTs were investigated, a... Zn-montmorillonites(Zn-MMTs) as antibacterial compounds were prepared by an ion-exchange reaction. The reaction time, initial pH value and molar ratios of CEC influencing zinc content in Zn-MMTs were investigated, and Zn-MMTs were characterized by means of EDX, XRD, XPS, and SEM. The results of bacterial growth tests were confirmed by determination of the minimum inhibition concentrations (MICs) and minimum bactericidal concentrations (MBCs). The experimental results show that the zinc is confirmed as bivalent zinc state, the d001 basal spacing of Zn-MMTs is enlarged with the enhancement of the zinc content, and the particles of Zn-MMTs are formed with irregular shape. Moreover, the antibacterial activity of Zn-MMTs increases with increasing the zinc content, and Zn-MMT-3 containing 6.76 mass% of zinc exhibits optimum antibacterial activity against Escherichia coli and Staphylococcus aureus. 展开更多
关键词 Zn^2+-exchanged montmorillonites PREPARATION chemical composition MICROSTRUCTURE antibacterial activity
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Changes in soluble interleukin-2 receptor level in serum and Na^+ -K^+ -exchanging ATPase activity in semen of infertile men caused by antisperm antibody 被引量:8
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作者 Jiang NI Qing-Lei LI +2 位作者 Wei ZHANG Jian-Song XIE Shu-Ling BIAN Department of Physiology, Harbin Medical University, Harbin 150086, China 《Asian Journal of Andrology》 SCIE CAS CSCD 2000年第2期151-153,共3页
Aim: To explore the possible mechanisms of male infertility caused by antisperm antibody (AsAb). Methods: Thesoluble interleukin-2 receptor (sIL-2R) level in serum was analyzed by ELISA and Na^+ -K^+ -exchanging ATPas... Aim: To explore the possible mechanisms of male infertility caused by antisperm antibody (AsAb). Methods: Thesoluble interleukin-2 receptor (sIL-2R) level in serum was analyzed by ELISA and Na^+ -K^+ -exchanging ATPase activi-ty in semen by phosphorus (Pi) assay. Results: The slL-2R level in serum was significantly higher and the Na^+ -K^+ -exchanging ATPase activity in semen significantly lower in AsAb positive infertile men when compared with thecontrols. Conclusion: The AsAb titer varies with the slL-2R level in serum. A decrease in Na^+ -K^+ -exchangingATPase activity in semen may play a role in male infertility caused by AsAb. 展开更多
关键词 male infertility antisperm antibodies soluble interleukin-2 receptor Na^+-K^+-exchanging ATPase
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惟一强*-clean环
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作者 王秀兰 李春娜 《湖北大学学报(自然科学版)》 CAS 2016年第3期182-186,266,共6页
*-环R被称为惟一强*-clean环,如果R中每个元素都可以惟一表示成可交换的单位和投射的和.本文中给出一个*-环是惟一强*-clean环的等价条件并给出几个惟一强*-clean环的例子.
关键词 惟一*-exchange 强*-clean环 惟一强*-clean环
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