Many approaches to neurodegenerative diseases that focus on amyloid-βclearance and gene therapy have not been successful.Some therapeutic applications focus on enhancing neuronal cell survival during the pathogenesis...Many approaches to neurodegenerative diseases that focus on amyloid-βclearance and gene therapy have not been successful.Some therapeutic applications focus on enhancing neuronal cell survival during the pathogenesis of neurodegenerative diseases,including mitochondrial dysfunction.Plasma membrane(PM)redox enzymes are crucial in maintaining cellular physiology and redox homeostasis in response to mitochondrial dysfunction.Neurohormetic phytochemicals are known to induce the expression of detoxifying enzymes under stress conditions.In this study,mechanisms of neuroprotective effects of 4-hydroxycinnamic acid(HCA)were examined by analyzing cell survival,levels of abnormal proteins,and mitochondrial functions in two different neuronal cells.HCA protected two neuronal cells exhibited high expression of PM redox enzymes and the consequent increase in the NAD^(+)/NADH ratio.Cells cultured with HCA showed delayed apoptosis and decreased oxidative/nitrative damage accompanied by decreased ROS production in the mitochondria.HCA increased the mitochondrial complexes I and II activities and ATP production.Also,HCA increased mitochondrial fusion and decreased mitochondrial fission.Overall,HCA maintains redox homeostasis and energy metabolism under oxidative/metabolic stress conditions.These findings suggest that HCA could be a promising therapeutic approach for neurodegenerative diseases.展开更多
In aquaculture,high-density seaweed farming brings higher economic benefits but also increases outbreaks of diatom felt.The effective control of diatom felt in high-density seaweed farming has always been a research h...In aquaculture,high-density seaweed farming brings higher economic benefits but also increases outbreaks of diatom felt.The effective control of diatom felt in high-density seaweed farming has always been a research hotspot.This study selected two potential allelochemicals 2-hydroxycinnamic acid and quinic acid to explore their effects on a diatom Nitzschia closterium and an economic seaweed Monostroma nitidum.The results showed that 2-hydroxycinnamic acid had better inhibitory effects than quinic acid on the growth,pigment content and photosynthetic efficiency of N.closterium.Their half-maximal inhibitory concentrations at 120 h(IC_(50–120 h))were 0.9000 and 1.278 mM,respectively.Additionally,these allelochemicals had limited inhibitory effects on the growth,pigment content and photosynthetic efficiency of M.nitidum before 24 h.To further explore the allelopathic effect of these chemicals,this study focused on the photosystem II energy fluxes of N.closterium.It was found that 3 mM 2-hydroxycinnamic acid could destroy the whole photosynthetic system by devastating the PSII reaction centre(RC)before 24 h;however,the same concentration of quinic acid could only down-regulate the electron transport efficiency by changing the effective antenna size of an active RC and downregulating the PSII reaction centre density.These experimental results are expected to provide a new strategy to control diatom felt blooms on the high-density seaweed farming areas.展开更多
The reaction of Ni（CH3COO）2.6H2O, 4-hydroxycinnamic acid （Hhca） and 4,4＇-bipyridine （bipy） ligand in water-solution afforded a mononuclear complex [Ni（H2O）4（4,4＇-bipy）2]（hca）2 which was characterized by ...The reaction of Ni（CH3COO）2.6H2O, 4-hydroxycinnamic acid （Hhca） and 4,4＇-bipyridine （bipy） ligand in water-solution afforded a mononuclear complex [Ni（H2O）4（4,4＇-bipy）2]（hca）2 which was characterized by IR, elemental analysis, TGA and single-crystal X-ray diffraction. X-ray crystallography analysis reveals that the complex is of triclinic, space group PI with a = 7.0238（14）, b = 7.2934（11）, c = 17.186（4） A, α = 87.073（13）, β = 84.354（10）, γ = 81.961（12）°, V= 866.9（3） A^3, Z = 1, Dc = 1.474 g/cm^3, F（000） = 402,μ = 62.7 mm^-1, R = 0.0411, wR = 0.0862 and GOF = 1.048. In solid state, the cation [Ni（H2O）4（4,4＇-bpy）2]^2＋ connects six nearby hca counter anions via O-H…O hydrogen bonds to form an extended 2D lattice framework and the O-…N hydrogen bonds finally link the 2D layers into an infinite 3D network.展开更多
基金supported by the National Research Foundation of Korea(NRF)of the Korean Government(NRF-2021R1F1A1051212)by Logsynk Co.Ltd.(2-2021-1435-001).
文摘Many approaches to neurodegenerative diseases that focus on amyloid-βclearance and gene therapy have not been successful.Some therapeutic applications focus on enhancing neuronal cell survival during the pathogenesis of neurodegenerative diseases,including mitochondrial dysfunction.Plasma membrane(PM)redox enzymes are crucial in maintaining cellular physiology and redox homeostasis in response to mitochondrial dysfunction.Neurohormetic phytochemicals are known to induce the expression of detoxifying enzymes under stress conditions.In this study,mechanisms of neuroprotective effects of 4-hydroxycinnamic acid(HCA)were examined by analyzing cell survival,levels of abnormal proteins,and mitochondrial functions in two different neuronal cells.HCA protected two neuronal cells exhibited high expression of PM redox enzymes and the consequent increase in the NAD^(+)/NADH ratio.Cells cultured with HCA showed delayed apoptosis and decreased oxidative/nitrative damage accompanied by decreased ROS production in the mitochondria.HCA increased the mitochondrial complexes I and II activities and ATP production.Also,HCA increased mitochondrial fusion and decreased mitochondrial fission.Overall,HCA maintains redox homeostasis and energy metabolism under oxidative/metabolic stress conditions.These findings suggest that HCA could be a promising therapeutic approach for neurodegenerative diseases.
基金sponsored by the Ph.D.Start-Up Foundation of Guangdong Ocean University(R19049)Science and Technology Planning Project of Guangdong(2017A030303078)Undergraduate Start-Up Foundation of Guangdong Ocean University(580520135 and 570119011).
文摘In aquaculture,high-density seaweed farming brings higher economic benefits but also increases outbreaks of diatom felt.The effective control of diatom felt in high-density seaweed farming has always been a research hotspot.This study selected two potential allelochemicals 2-hydroxycinnamic acid and quinic acid to explore their effects on a diatom Nitzschia closterium and an economic seaweed Monostroma nitidum.The results showed that 2-hydroxycinnamic acid had better inhibitory effects than quinic acid on the growth,pigment content and photosynthetic efficiency of N.closterium.Their half-maximal inhibitory concentrations at 120 h(IC_(50–120 h))were 0.9000 and 1.278 mM,respectively.Additionally,these allelochemicals had limited inhibitory effects on the growth,pigment content and photosynthetic efficiency of M.nitidum before 24 h.To further explore the allelopathic effect of these chemicals,this study focused on the photosystem II energy fluxes of N.closterium.It was found that 3 mM 2-hydroxycinnamic acid could destroy the whole photosynthetic system by devastating the PSII reaction centre(RC)before 24 h;however,the same concentration of quinic acid could only down-regulate the electron transport efficiency by changing the effective antenna size of an active RC and downregulating the PSII reaction centre density.These experimental results are expected to provide a new strategy to control diatom felt blooms on the high-density seaweed farming areas.
基金Supported by the National Natural Science Foundation of China (20571054 and 20476066), the NSF of Jiangsu Province (BK2005031) and Project of High Technology of Jiangsu Province (BG2005021)
文摘The reaction of Ni（CH3COO）2.6H2O, 4-hydroxycinnamic acid （Hhca） and 4,4＇-bipyridine （bipy） ligand in water-solution afforded a mononuclear complex [Ni（H2O）4（4,4＇-bipy）2]（hca）2 which was characterized by IR, elemental analysis, TGA and single-crystal X-ray diffraction. X-ray crystallography analysis reveals that the complex is of triclinic, space group PI with a = 7.0238（14）, b = 7.2934（11）, c = 17.186（4） A, α = 87.073（13）, β = 84.354（10）, γ = 81.961（12）°, V= 866.9（3） A^3, Z = 1, Dc = 1.474 g/cm^3, F（000） = 402,μ = 62.7 mm^-1, R = 0.0411, wR = 0.0862 and GOF = 1.048. In solid state, the cation [Ni（H2O）4（4,4＇-bpy）2]^2＋ connects six nearby hca counter anions via O-H…O hydrogen bonds to form an extended 2D lattice framework and the O-…N hydrogen bonds finally link the 2D layers into an infinite 3D network.
