葛根芩连汤调控FoxO1/MARCH1通路对小鼠胰岛素抵抗的改善作用

目的探讨葛根芩连汤通过调控肝脏叉头框蛋白O1(FoxO1)/膜相关环状蛋白1(MARCH1)通路对高脂饮食诱导的小鼠胰岛素抵抗的影响。方法小鼠给予高脂饲料喂养12周建立胰岛素抵抗模型,将30只造模成功小鼠随机分为模型组、葛根芩连汤组(15 g/kg)、吡格列酮(阳性药)组(20 mg/kg),每组10只,另取10只正常小鼠给予普通饲料喂养为正常组,连续给予相应药物灌胃8周,记录各组小鼠体质量。全自动生化分析仪检测检测甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)水平,ELISA法检测空腹血清胰岛素(FINS)水平并计算稳态模型胰岛素抵抗指数(HOMA-IR),检测腹腔内葡萄糖耐量(IPGTT)并计算葡萄糖曲线下面积(AUC),HE染色观察肝组织病理学改变,RT-qPCR法检测肝组织FoxO1、MARCH1、INSR、IRS1 mRNA表达,Western blot法检测肝组织FoxO1、Ac-FoxO1、MARCH1、INSRα、INSRβ、IRS1、p-IRS1蛋白表达。结果与正常组比较,模型组小鼠体质量、FINS、TG、TC、LDL-C、HOMA-IR、AUC均升高(P<0.01),肝组织细胞结构紊乱,边界轮廓不清,排列散乱,细胞肿大且大小不一,肝细胞内可见大量脂质空泡,少量肝细胞出现点状坏死,肝组织FoxO1、MARCH1 mRNA表达升高(P<0.01),INSR、IRS1 mRNA表达降低(P<0.01),肝组织Ac-FoxO1、MARCH1、INSRα蛋白表达升高(P<0.01),FoxO1、INSRβ、IRS1、p-IRS1蛋白表达降低(P<0.01);与模型组比较,葛根芩连汤组小鼠FINS、TG、TC、LDL-C、HOMA-IR、AUC均降低(P<0.01),肝组织细胞形态趋于正常,脂质空泡数目明显减少,肝组织FoxO1、MARCH1 mRNA表达降低(P<0.01),INSR、IRS1 mRNA表达升高(P<0.01),肝组织Ac-FoxO1、MARCH1蛋白表达降低(P<0.01),FoxO1、INSRβ、IRS1、p-IRS1蛋白表达均升高(P<0.01),而INSRα蛋白表达无明显变化(P>0.05)。结论葛根芩连汤可以减轻肝脏脂肪变性,改善胰岛素抵抗,其机制可能与抑制FoxO1/MARCH1通路,增加胰岛素受体及胰岛素受体底物的表达有关。

Improvement of heat-tolerance by training I. Evaluation of effects of long-distance running and march training on heat-tolerance

in order to verify the heat-tolerance effect, two trainings, 90 min marching with load (WBGT 24. 6～35.6℃) and 10 km running (WBGT 25.0～31.1℃) were performed in laboratory and field under hot climate.Ten to twelve times (days) of training were carried out

基于改进Marching Cubes算法的雷达气象数据三维重建

在气象领域中,多普勒天气雷达探测到的气象数据采用以雷达站点为原点的三维极坐标系进行存储,具有形状不规则、数据量大等特征。Marching Cubes(MC)算法是三维重建中的经典算法,但应用于气象领域时存在重建效率低下和不能直接处理气象数据的缺点。为了实现气象数据的三维重建,基于MC算法提出雷达数据归一化处理与状态标记判别算法NBV-MC。该算法根据雷达基数据文件的特点对其进行归一化预处理,使用雷达基数据构建拟梯形六面体体素,对每一个六面体体素进行状态标记,在遍历六面体体素时动态判别其是否需要处理。实验结果表明,NBV-MC算法不仅解决了由于气象数据具有不规则性而不能直接用于MC算法的问题,而且可以在保证数据真实性和重建效果的情况下有效减少绘制等值面所需要的三角面片数量,提高重建速度。与MC算法相比,NBV-MC算法的重建效率提升了77.70%以上,有利于实时场景交互,便于气象研究人员直接分析雷达数据。

可兼容四种March系列算法的PMBIST电路设计

存储器是系统级芯片(System on chip,SoC)中最重要的组成部分之一,也是最容易出现故障的部件。存储器故障可能会导致整个SoC失效,对存储器进行充分的测试和验证是至关重要的。目前,主流的存储器测试方法是采用存储器内建自测试(Memory build-in-self test,MBIST)技术,传统的可测性技术采用单一的测试算法进行测试,为了满足不同类型存储器的测试需求以及不同工艺制造阶段的测试强度,需要使用不同类型的测试算法进行测试。结合存储器常见的故障模型以及多种测试算法,设计了具有较高灵活性和可扩展性的可编程存储器内建自测试(Programmable memory built-in-self test,PMBIST)电路,可兼容四种不同的March系列算法进行存储器内建自测试,采用寄存器传输语言(Reigster transfer language,RTL)级代码的编写方式,针对静态随机存储器(Static random-access memory,SRAM)采用不同March系列测试算法进行仿真,并以常用的March C+算法为例进行说明。仿真结果表明,所设计的PMBIST电路可对四种不同的March算法进行测试,满足不同类型存储器的内建自测试需求。

Research on Optimizing the Construction of the Long March National Cultural Park from the Perspective of Cultural Route Heritage Protection

The idea of cultural route heritage protection expands the protection theory for world cultural heritage.The Long March route carries the prominent characteristic of cultural route heritage.By actively building the Long March National Cultural Park,China has strengthened the overall protection and integrated utilization of the Long March cultural heritage,upgraded the hardware facilities for exhibition,and investigated ways to protect cultural route heritage with Chinese characteristics.During the construction of the Park,it is necessary to draw on the concept and norms of the cultural route heritage protection,optimize the construction approach,and make the construction of the Park more in line with the linear cultural characteristics of Long March heritage.Specifically,it is proposed to improve the Long March cultural route heritage protection system oriented towards“authenticity”protection,to deeply tap and interpret the prominent universal worth of the Long March route,augmenting the international dissemination of the Long March culture in an innovative way,and realizing a more scientific and effective protection and utilization of Long March cultural resources.

Spatio-Temporal Assessment of the Performance of March to May 2020 Long Rains and Its Socio-Economic Implications in Northern Coast of Tanzania

The spatio-temporal analysis of the performance of the March to May (MAM) 2020 rainfall and its societal implications to Northern Coastal Tanzania (NCT) including Zanzibar was investigated. The uniqueness of the October to December, 2019 (OND) rainfall and the extension of the January to February, 2020 rainfall in Zanzibar which coincided with MAM 2020 rainfall was among the issues which prolonged MAM 2020 rainfall in NCT and Zanzibar. The National Center for Environmental Prediction (NCEP) in collaboration with National Center for Atmospheric Research (NCAR). Reanalysis 1 datasets of u (zonal) and v (meridional) winds, sea surface temperatures anomalies, relative humidity, amount of precipitable water and ocean net flux were analyzed. Other datasets include the Tanzania Meteorological Authority (TMA) observed rainfall records, maximum and minimum temperatures. Moreover, TMA and Intergovernmental Climate Prediction and Analysis Center (ICPAC). MAM 2020 rainfall and temperature forecast reports were interpreted. Gridded and observed datasets were calculated into monthly and seasonal averages. As for observed data, long-term monthly and MAM percentage changes were calculated. Besides, the correlation between rainfall anomalies with an area-averaged SSTA for defined regions and stations in Zanzibar was performed. Lastly, the calculated monthly and seasonal rainfall was compared to MAM periods of 2016, 2017, 2018 and 2019. Results revealed that consecutive five MAM seasonal rainfall was among the highest ones in records with that of 2020 being exceptional. These MAM seasons had percentage contribution ranged from 68% - 212%, 150% - 304%, 22% - 163% and 57% - 170% for stations in Zanzibar and 130% - 230%, 57% - 168% and 230% - 706% for NCT station, respectively. Compared to previous MAM seasons of 2016-2019, MAM 2020 rainfall season was spatially well distributed in our study area with rainfall ranging from 1200 to 2100 mm and up to 900 in most Zanzibar and NCT stations. Indeed, the study revealed that the observed highest rainfall and flooding was enhanced by wet seasons of MAM 2019, OND 2019 and DFJ (2019-2020). Other dynamics which accelerated MAM 2020 rainfall were the higher SSTA ranged from 0.5°C - 1.5°C and 1.5°C - 2.5°C over Southwestern Indian Ocean (SWIO) and coastal Tanzania and the increased trend of area-averaged SSTA on various SWIO blocks. Besides, parameters including Rhum, PWR and wind regimes were supporting the MAM 2020 rainfall. The socio-economic implications of these rains were strong and spatially well distributed in Zanzibar. For instance, a death toll of about 10 people, a great number of road culverts were washed away, and about 3600 houses were fallen or damaged, leading to a significant number of homeless people. As for NCT, the catastrophes include loss of lives, increased water levels over Lake Victoria leading to flooded islands and re allocation of more than 1000 people. In Kenya, more than 116 people died and 40,000 people were displaced. Conclusively, the study has shown the uniqueness (i.e., strength and societal implications) of MAM 2020 compared to other seasons;hence more studies on understanding the factors affecting extreme rainfall seasons in East Africa are required.

Construction and validation of somatic mutation-derived long noncoding RNAs signatures of genomic instability to predict prognosis of hepatocellular carcinoma

BACKGROUND Long non-coding RNAs(LncRNAs)have been found to be a potential prognostic factor for cancers,including hepatocellular carcinoma(HCC).Some LncRNAs have been confirmed as potential indicators to quantify genomic instability(GI).Nevertheless,GI-LncRNAs remain largely unexplored.This study established a GI-derived LncRNA signature(GILncSig)that can predict the prognosis of HCC patients.AIM To establish a GILncSig that can predict the prognosis of HCC patients.METHODS Identification of GI-LncRNAs was conducted by combining LncRNA expression and somatic mutation profiles.The GI-LncRNAs were then analyzed for functional enrichment.The GILncSig was established in the training set by Cox regression analysis,and its predictive ability was verified in the testing set and TCGA set.In addition,we explored the effects of the GILncSig and TP53 on prognosis.RESULTS A total of 88 GI-LncRNAs were found,and functional enrichment analysis showed that their functions were mainly involved in small molecule metabolism and GI.The GILncSig was constructed by 5 LncRNAs(miR210HG,AC016735.1,AC116351.1,AC010643.1,LUCAT1).In the training set,the prognosis of high-risk patients was significantly worse than that of low-risk patients,and similar results were verified in the testing set and TCGA set.Multivariate Cox regression analysis and stratified analysis confirmed that the GILncSig could be used as an independent prognostic factor.Receiver operating characteristic curve analysis of the GILncSig showed that the area under the curve(0.773)was higher than the two LncRNA signatures published recently.Furthermore,the GILncSig may have a better predictive performance than TP53 mutation status alone.CONCLUSION We established a GILncSig that can predict the prognosis of HCC patients,which will help to guide prognostic evaluation and treatment decisions.

An Analysis of the Festivals and Holidays Celebrated by the Red Army During the Long March

The Long March of the Chinese Workers’and Peasants’Red Army is hailed as the“Red Ribbon on the Earth.”The Long March was characterized by revolutionary heroism and,at the same time,was no lack of revolutionary romanticism.This arduous journey also presented two difficult questions:whether to celebrate festivals and holidays or not and whether to celebrate them simply or grandly.By exploring such trade-offs,we can appreciate a strong sense of national identity among the Red Army soldiers as idealists,grasp more historical details of the world-shattering Long March,and feel the resilience and greatness of the Chinese nation.

Elimination of the Potential Hazard of the Long March 4 Launch Vehicle's In-orbit Breakup

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CASC Eyes the Long March Vehicle's Reliability Improvement

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Long March Rockets Complete 2018 Missions with 37 Record-Setting Launches

A LM-2D/Yuanzheng 3 launch vehicle successfully launched 6 Yunhai 2 satellites and one test microsatellite named'Chongqing'into their preset orbits,concluding China’s final orbital launch of2018.The launch took place at 16:00 Beijing time on December 29,2018 from the Jiuquan Satellite Launch Center.

Flight Record of the Long March Series of Launch Vehicles

(Continued) The 75th Launch On December 30, 2003, the LM-2C/SM launch vehicle launched theTance 1 (TC-1) satellite into orbit from the Xichang Satellite Launch Center. The satellite entered a super geosynchronous orbit . Launch Site: Xichang Satellite Launch Center Launch Result: Success At 03:06 Beijing time on December 30, the LM-2C/SM launch vehicle lifted off into space with TC-1 (equatorial satellite) and precisely sent the satellite into

Flight Record of the Long March Series of Launch Vehicles

(Continued)The 71st Launch On October 15,2003,China's first manned spaceship,Shenzhou 5,was launched by a LM-2F launch vehicle.The first Chinese astronaut Yang Liwei stayed in space for 21 hours and landed back on the Earth safely on October 16。

Research on Overall Optimization Technology of Long March 6A Launch Vehicle

On March 29,2022,the Long March 6A launch vehicle developed by the Shanghai Academy of Space-flight Technology successfully made its maiden flight at the Taiyuan Satellite Launch Center.The Long March 6A launch vehicle was China’s first solid bundled rocket.It uses non-toxic and non-polluting propellants,and has a 700 km sun-synchronous orbit carrying capacity of no less than 4.5 t,which is more than 50%higher than that of a LM-4C launch vehicle.During the development of the LM-6A launch vehicle,the matching relationship between systems and the overall performance of the entire rocket improved significantly through continuous overall optimization work.This paper introduces the typical overall optimization work during the development of the LM-6A launch vehicle.

Long March 6A and Its Technical Characteristics

China’s first solid-liquid bundled launch vehicle,the Long March 6 A(LM-6 A)successfully adopted a number of key new technologies,launching the Pujiang 2 and Tiankun 2 satellites precisely into their predetermined orbits,thus demonstrating that China has mastered solid-liquid bundled launch vehicle technology and has a new member in the new generation launch vehicle family.For the first time in China,a booster adopted a 2 m-diameter solid motor and a 270 V,20 k W high-voltage and high-power electric servo system was used to control the swing of the solid motor nozzles.A health diagnosis system for the core first stage engine and unattended automation technology were also adopted for the first time.

MARCH2和CFTR在结直肠癌中的表达及临床意义

目的:探讨MARCH2和CFTR在结直肠癌中的蛋白表达及与临床病理参数的相关性。方法:采用免疫组织化学SP法检测125例结直肠癌和30例正常结直肠黏膜组织中MARCH2和CFTR的表达,并复习相关文献。结果:在125例结直肠癌组织中,MARCH2和CFTR的阳性表达率分别为84.8%和8.8%,而在非肿瘤性肠组织中,这两种表达率的阳性率分别为6.7%和90.0%。MARCH2的阳性表达与肿瘤大小、深部浸润、淋巴结转移和TNM分期密切相关。此外,CFTR表达的缺失与分化不良、淋巴结转移和TNM分期显著相关。此外,MARCH2和CFTR表达之间存在显著负相关。结论:MARCH2在结直肠癌中高度表达,与肿瘤大小、深部浸润、淋巴结转移和TNM分期等预后不良参数有关。相比之下,CFTR在结直肠癌中几乎不表达,CFTR表达缺失与分化不良、淋巴结转移和TNM分期显著相关。MARCH2可能通过与CFTR的相互作用在结直肠癌的发展中发挥重要作用。它们可能成为结直肠癌诊断、治疗和预后评估的分子标志物。Aims: This study aimed to investigate whether the expressions of MARCH2 and CFTR in colorectal carcinomas were associated with clinicopathological parameters. Methods: The expressions of MARCH2 and CFTR were examined in 125 cases of colorectal carcinomas and 30 normal colorectal tissues using immunohistochemical staining. Results: The positive rates of MARCH2 and CFTR expressions in 125 cases of colorectal carcinoma were 84.8% and 8.8% respectively, whereas the positive expression rates of MARCH2 and CFTR in non-tumor colorectal tissues were 6.7% and 90.0% respectively. The positive expressions of MARCH2 were closely related to tumor size, deep invasion, lymph node metastasis and advanced TNM stage. In addition, loss of expression of CFTR was significantly associated with poor differentiation, lymph node metastasis and advanced TNM stage. Furthermore, there were significant negative correlations between MARCH2 and CFTR expression. Conclusions: MARCH2 is highly expressed in colorectal carcinomas, and is associated with poor prognostic parameters such as tumor size, deep invasion, lymph node metastasis and advanced TNM stage. In contrast, CFTR barely expressed in colorectal carcinomas, and loss of expression of CFTR was significantly associated with poor differentiation, lymph node metastasis and advanced TNM stage. MARCH2 may play an important role in the development of colorectal carcinoma through interaction with CFTR. They may become molecular markers for diagnosis, treatment and prognostic evaluation of colorectal carcinomas.

Flight Record of the Long March Series of Launch Vehicles

(Continued)THE 67TH LAUNCHOn May 15, 2002, a LM-4B launch vehicle lifted off with FY-1D meteorological satellite and HY-1A oceanic satellite from Tai-yuan Satellite Launch Center and

Flight Record of the Long March Series of Launch Vehicles

(Continued)THE 67TH LAUNCHOn May 15, 2002, a LM-4B launch vehicle lifted off with FY-1D meteorological satellite and HY-1A oceanic satellite from Tai-yuan Satellite Launch Center and

Flight Record of the Long March Series of Launch Vehicles

(Continued) THE 59TH LAUNCH On November 20,1999,a LM-2F launch vehicle lifted off with China’s indigenous Shenzhou 1 experimental spaceship from Jiuquan Satellite Launch Center.Shenzhou 1 returned to Earth on November 21 after 21 hours of in-orbit operation and circling the Earth 14 times.

MARCH2多克隆抗体的制备与鉴定及其在组织和细胞系中的表达与亚细胞结构中的定位

目的:探讨MARCH2多克隆抗体的制备、鉴定与纯化的方法以及其在组织和细胞系中的表达情况、在亚细胞结构中的定位情况。方法:利用DNAstar软件对MARCH2蛋白的抗原性等进行分析,化学合成MARCH2短肽,制备成完全抗原免疫家兔,获取血清,纯化,用Western blot、Elisa、免疫荧光进行鉴定。用RT-PCR检测MARCH2在细胞系中的表达情况,用Western blot检测MARCH2在组织中的表达情况。用免疫荧光法及激光共聚焦显微镜分析检测MARCH2在亚细胞结构中的定位。结果:成功制备完全抗原免疫家兔,纯化出多克隆抗体,用Western blot、Elisa、免疫荧光法证实多克隆抗体制备成功。利用半定量RT-PCR方法,在32种细胞系中检测了MARCH2 mRNA的表达水平,结果显示,MARCH2呈广泛表达,在HeLa、U2OS、HCT116、COS7细胞中表达最高,在SKBR3、HGC-27、MGC-803细胞中低表达。利用组织芯片及免疫组织化学方法进一步分析了其在正常组织及肿瘤组织中的表达情况,染色结果显示,MARCH2呈广泛表达,在前列腺、肝组织中呈高表达,在横纹肌、甲状腺组织中呈低表达,主要在胞浆中呈现弥漫均匀细颗粒状分布。此外,MARCH2表达因肿瘤类型的不同有差异。与对应的正常组织相比,MARCH2在某些肿瘤(如前列腺癌、肝癌)组织中表达降低,而在某些肿瘤(如食管癌、结肠癌)组织中表达增高。用免疫荧光法及激光共聚焦显微镜分析检测MARCH2在亚细胞结构中的定位,发现MARCH2与内质网、高尔基体共定位,与溶酶体、内体部分共定位,与线粒体没有共定位。结论:成功获得了MARCH2多克隆抗体,为进一步研究MARCH2蛋白的功能奠定了基础。MARCH2在不同类型肿瘤中的差异表达及其在亚细胞结构中的定位高度提示MARCH2在肿瘤发生发展中具有重要的潜在应用价值。Aims: To explore the preparation, identification, and purification methods of MARCH2 polyclonal antibodies, as well as their expression and subcellular structural localization in tissues and cell lines. Methods: DNAstar software was used to analyze the antigenicity of MARCH2 protein, and MARCH2 short peptides were chemically synthesized to prepare complete antigen-immunized rabbits. Serum was obtained, purified, and identified by Western blot, Elisa, and immunofluorescence. RT-PCR was used to detect the expression of MARCH2 in cell lines, and Western blot was used to detect the expression of MARCH2 in tissues. Immunofluorescence and confocal laser microscopy were used to analyze and detect the localization of MARCH2 in subcellular structures. Results: Fully antigen-immunized rabbits were successfully prepared, and polyclonal antibodies were purified. Western blot, Elisa, and immunofluorescence were used to confirm the successful preparation of polyclonal antibodies. Using semi-quantitative RT-PCR method, the expression level of MARCH2 mRNA was detected in 32 cell lines. The results showed that MARCH2 was widely expressed, with the highest expression in HeLa, U2OS, HCT116, and COS7 cells, and low expression in SKBR3, HGC-27, and MGC-803 cells. The expression of MARCH2 in normal and tumor tissues was further analyzed using tissue chips and immunohistochemical methods. The staining results showed that MARCH2 was widely expressed, with high expression in prostate and liver tissues, low expression in striated muscle and thyroid tissues, and mainly distributed in a diffuse and uniform granular form in the cytoplasm. In addition, MARCH2 expression varies depending on the type of tumor. Compared with corresponding normal tissues, MARCH2 expression is reduced in certain tumor tissues (such as prostate cancer and liver cancer), while it is increased in certain tumor tissues (such as esophageal cancer and colon cancer). Using immunofluorescence and laser confocal microscopy to analyze and detect the localization of MARCH2 in subcellular structures, it was found that MARCH2 was co-localized with the endoplasmic reticulum and Golgi apparatus, with lysosomes and endosomes partially, but not with mitochondria. Conclusions: MARCH2 polyclonal antibodies have been successfully obtained, laying the foundation for further research on the function of MARCH2 protein. The differential expression of MARCH2 in different types of tumors and its localization in subcellular structures highly suggest that MARCH2 has important potential application value in tumor occurrence and development.