The paper was to establish a simple and sensitive HPLC method for determination of berbefine hydrochloride content in Qingwen Baidu granule. The HPLC system consisted of Stable Bond - C18 column （4.6 × 150 mm, ...The paper was to establish a simple and sensitive HPLC method for determination of berbefine hydrochloride content in Qingwen Baidu granule. The HPLC system consisted of Stable Bond - C18 column （4.6 × 150 mm, 5um） and with a mobile phase of acetonitrile -0.05mol/L potassium dihydrogen phosphate solution （50：50,v：v） mixture. Berberine hydrochloride was detected at the wavelength of 345 nm, with a flow rate of 1.0 ml/min and a column temperature of 30 ℃. The excipients and solvents in the granule could be well separated from the drug under such a designated chromatogram condition and did not interfere with the assay. A good linear relationship was found between peak area and the concentration of berberine hydrochloride in the range of 16.0 -48.0 I^g/mL. The average recovery of berberine hydrochloride was 99.09%. The established approach was specific, accurate, reliable, prompt, sensitive and applicable, and could be used to control the quality of Qingwen Baidu granule.展开更多
[ Objective ] We aimed to establish the identification method of gardenoside and sarsasapogenin in Qingwen baidu granules. [ Method] With ethyl ace- tate-acetone-formic acid-water （10：7：2：0.5） as the developer an...[ Objective ] We aimed to establish the identification method of gardenoside and sarsasapogenin in Qingwen baidu granules. [ Method] With ethyl ace- tate-acetone-formic acid-water （10：7：2：0.5） as the developer and 10% sulfuric acid ethanol solution as the chromogenic reagent, the samples were heated at 100 ~C until the spots were clearly visible, and the existence of gardenoside was checked under natural light. With toluene-acetone（9：l ） as the developer and 5% vanillin sulfuric acid solution as the chromogenic reagent, the samples were heated at 105 ~C until the spots were clearly visible, and the existence of sarsasapogenin was checked under natural light. [Result] Qingwen baidu granules had the same spots with gardenoside and sarsasapogenin at the same Rfvalue under natural light. [ Conclusion] A TLC method detecting gardenoside and sarsasapogenin in Qingwen baidu granules was established, which had good specificity and repeatability, suitable for rapid detection of gardenoside and sarsasapogenin.展开更多
[ Objective] The paper was to establish the identification method of Coptis chinensis and Forsythia suspensa in Qingwen baidu granules. [ Method ] With benzene-ethyl acetate-methanol-isopropanol-cancentrated ammonium ...[ Objective] The paper was to establish the identification method of Coptis chinensis and Forsythia suspensa in Qingwen baidu granules. [ Method ] With benzene-ethyl acetate-methanol-isopropanol-cancentrated ammonium liquid （ 12：6：3：3：1 ） as the developer, the samples were outspread in the cylinder with sat- urated ammonia steam by thin layer chromatography （TLC）, and the existence of C. chinensis and berberine hydrochloride was checked under UV lamp. With tri- chloromethane-methanol （5：1） as the developer and 10% sulfuric acid ethanol solution as the chromogenic reagent, the samples were heated at 105 ℃ for 5 min to check the existence of F. suspense under natural light. [Result] Qingwen baidu granules had the same spots with C. chinensis control and berberine hydrochloride at the same Rf value under 365 nm UV lamp; Qingweu baidu granules had the same spots with F. suspensa control at the same Rfvalue under natural light. [ Condusion] A TLC method detecting C. chinensis and F. suspensa in Qingwen baidu granules was established, which had good specificity and repeatability, suitable for rapid detection of C. chinensis and F. suspensa. It could be well applied to control the quality of Qingwen baidu granule.展开更多
Background:Sepsis-induced coagulopathy and multiple organ dysfunction syndromes are the leading causes of death in patients with sepsis.Qingwen Baidu decoction(QWBD)can effectively improve the clinical manifestations ...Background:Sepsis-induced coagulopathy and multiple organ dysfunction syndromes are the leading causes of death in patients with sepsis.Qingwen Baidu decoction(QWBD)can effectively improve the clinical manifestations of sepsis and ease inflammation,but its effects on coagulation functions and multiple organ injuries remain unclear.Methods:100 healthy,male Sprague-Dawley rats were randomly divided into the sham group,the cecal ligation and puncture(CLP)group,the low-dose QWBD group,and the high-dose QWBD group,with 25 rats in each group.The sepsis model was established using CLP.Blood was collected to measure platelet count,serum creatinine(Cr),blood urea nitrogen(BUN),alanine aminotransferase(ALT),and aspartate aminotransferase(AST)levels,as well as coagulation function.The total protein in bronchoalveolar lavage fluid(BALF)was determined in each group of rats.The lung,liver,and kidney tissues were harvested,and statistics were calculated on the wet-to-dry(W/D)weight ratio.Changes in histopathology and thrombin level were evaluated in each group.The remaining ten rats in each group were observed daily to record the number of surviving rats.Such observation was made consecutively for 7 days to calculate survival rates.Results:After model establishment,ALT,AST,Cr,and BUN levels were significantly elevated(P<0.01).The BALF protein content and lung W/D weight ratio were significantly increased(P<0.01).Furthermore,the survival rate of rats was significantly reduced in the CLP group compared with the sham group.After the treatment,rats in the high-dose QWBD group had lower ALT(P<0.05),AST(P<0.01),Cr(P<0.05),BUN(P<0.01)levels,lower BALF protein content(P<0.05)and lower lung W/D weight ratio(P<0.01)than the CLP group.However,rats in the high-dose QWBD group had significantly better pathological changes in the lung,liver,and kidney compared to the sham group.After the treatment,the platelet level in the peripheral blood was elevated(P<0.05)and both activated partial thromboplastin time and prothrombin time were significantly shortened(P<0.01).The fibrinogen level was significantly increased(P<0.01).Finally,thrombin positive expression areas in the lung,liver,and kidney were significantly decreased in the high-dose QWBD group.Conclusion:QWBD can improve coagulation disorders caused by sepsis and has a protective effect on multiple organ injuries in rats.展开更多
目的:通过观察清瘟败毒饮对脂多糖(LPS)诱导急性肺损伤(ALI)大鼠肺组织病理及核因子-κBp65(NF-κBp65)表达的影响,探讨清瘟败毒饮对ALI的治疗作用及干预机制。方法:选取健康雄性SD大鼠144只,随机分为6组,每组24只,分别为正常对照组,模...目的:通过观察清瘟败毒饮对脂多糖(LPS)诱导急性肺损伤(ALI)大鼠肺组织病理及核因子-κBp65(NF-κBp65)表达的影响,探讨清瘟败毒饮对ALI的治疗作用及干预机制。方法:选取健康雄性SD大鼠144只,随机分为6组,每组24只,分别为正常对照组,模型组,泼尼松组和清瘟败毒饮大、中、小剂量组。脂多糖溶液(2mg/kg)气管内滴注复制ALI大鼠模型,连续两天。各治疗组在第2次滴注完LPS溶液1h后,用生理盐水将相应的药物稀释至4mL灌胃,1天2次。各组分别于灌胃后24h、48h处死6只大鼠,于72h处死余下所有大鼠,并收集所需标本;通过镜下观察肺组织病理变化及采用W estern b lot方法检测肺组织中NF-κBp65的表达水平。结果:LPS致ALI大鼠肺组织肺泡结构破坏或实变,肺间质明显水肿,大量炎性细胞浸润,肺泡腔和肺间质内可见局灶性炎症细胞浸润和大量红细胞渗出,与同时相模型组比较,清瘟败毒饮大、中剂量组大鼠病理学炎症积分均降低非常显著(P<0.01),而小剂量组只有24h、48h有显著性降低或非常显著性降低(P<0.05或P<0.01);清瘟败毒饮各剂量治疗组中48h清瘟败毒饮大、中剂量组和72h各剂量组大鼠肺组织中NF-κBp65的表达均降低,与同时相模型组相比均有显著性差异或非常显著性差异(P<0.05或P<0.01)。结论:清瘟败毒饮能减轻LPS致ALI大鼠肺组织的损伤程度,在一定程度上减少炎症细胞在肺部积聚、浸润、渗出,起到比较明确的肺保护作用;清瘟败毒饮能有效减少LPS致ALI大鼠肺组织中NF-κBp65的表达,通过抑制NF-κB的活化和炎性细胞因子的生成,对炎症反应起到抑制作用;在LPS致ALI早期使用清瘟败毒饮,可有效减轻肺组织的损伤程度,减轻肺部的炎症反应。展开更多
基金Supported by Technology Innovation Project of Shandong Province(201210916001)
文摘The paper was to establish a simple and sensitive HPLC method for determination of berbefine hydrochloride content in Qingwen Baidu granule. The HPLC system consisted of Stable Bond - C18 column （4.6 × 150 mm, 5um） and with a mobile phase of acetonitrile -0.05mol/L potassium dihydrogen phosphate solution （50：50,v：v） mixture. Berberine hydrochloride was detected at the wavelength of 345 nm, with a flow rate of 1.0 ml/min and a column temperature of 30 ℃. The excipients and solvents in the granule could be well separated from the drug under such a designated chromatogram condition and did not interfere with the assay. A good linear relationship was found between peak area and the concentration of berberine hydrochloride in the range of 16.0 -48.0 I^g/mL. The average recovery of berberine hydrochloride was 99.09%. The established approach was specific, accurate, reliable, prompt, sensitive and applicable, and could be used to control the quality of Qingwen Baidu granule.
基金Supported by Technical Innovation Project of Shandong Province(201210916001)
文摘[ Objective ] We aimed to establish the identification method of gardenoside and sarsasapogenin in Qingwen baidu granules. [ Method] With ethyl ace- tate-acetone-formic acid-water （10：7：2：0.5） as the developer and 10% sulfuric acid ethanol solution as the chromogenic reagent, the samples were heated at 100 ~C until the spots were clearly visible, and the existence of gardenoside was checked under natural light. With toluene-acetone（9：l ） as the developer and 5% vanillin sulfuric acid solution as the chromogenic reagent, the samples were heated at 105 ~C until the spots were clearly visible, and the existence of sarsasapogenin was checked under natural light. [Result] Qingwen baidu granules had the same spots with gardenoside and sarsasapogenin at the same Rfvalue under natural light. [ Conclusion] A TLC method detecting gardenoside and sarsasapogenin in Qingwen baidu granules was established, which had good specificity and repeatability, suitable for rapid detection of gardenoside and sarsasapogenin.
基金Supported by Technical Innovation Project of Shandong Province(201210916001)
文摘[ Objective] The paper was to establish the identification method of Coptis chinensis and Forsythia suspensa in Qingwen baidu granules. [ Method ] With benzene-ethyl acetate-methanol-isopropanol-cancentrated ammonium liquid （ 12：6：3：3：1 ） as the developer, the samples were outspread in the cylinder with sat- urated ammonia steam by thin layer chromatography （TLC）, and the existence of C. chinensis and berberine hydrochloride was checked under UV lamp. With tri- chloromethane-methanol （5：1） as the developer and 10% sulfuric acid ethanol solution as the chromogenic reagent, the samples were heated at 105 ℃ for 5 min to check the existence of F. suspense under natural light. [Result] Qingwen baidu granules had the same spots with C. chinensis control and berberine hydrochloride at the same Rf value under 365 nm UV lamp; Qingweu baidu granules had the same spots with F. suspensa control at the same Rfvalue under natural light. [ Condusion] A TLC method detecting C. chinensis and F. suspensa in Qingwen baidu granules was established, which had good specificity and repeatability, suitable for rapid detection of C. chinensis and F. suspensa. It could be well applied to control the quality of Qingwen baidu granule.
基金supported by the National Key R&D Program of China(No.2018YFC1706500Nos.81973800)+3 种基金the Tianjin Scientific Research Project of Priority Area of Traditional Chinese Medicine(No.20170062022007)the Oncology Translational Medicine Seed Fund Project of Tianjin Medical University Cancer Hospital(No.1910)the Chunmiao Project of Tianjin First Center Hospital(No.2019CM15).
文摘Background:Sepsis-induced coagulopathy and multiple organ dysfunction syndromes are the leading causes of death in patients with sepsis.Qingwen Baidu decoction(QWBD)can effectively improve the clinical manifestations of sepsis and ease inflammation,but its effects on coagulation functions and multiple organ injuries remain unclear.Methods:100 healthy,male Sprague-Dawley rats were randomly divided into the sham group,the cecal ligation and puncture(CLP)group,the low-dose QWBD group,and the high-dose QWBD group,with 25 rats in each group.The sepsis model was established using CLP.Blood was collected to measure platelet count,serum creatinine(Cr),blood urea nitrogen(BUN),alanine aminotransferase(ALT),and aspartate aminotransferase(AST)levels,as well as coagulation function.The total protein in bronchoalveolar lavage fluid(BALF)was determined in each group of rats.The lung,liver,and kidney tissues were harvested,and statistics were calculated on the wet-to-dry(W/D)weight ratio.Changes in histopathology and thrombin level were evaluated in each group.The remaining ten rats in each group were observed daily to record the number of surviving rats.Such observation was made consecutively for 7 days to calculate survival rates.Results:After model establishment,ALT,AST,Cr,and BUN levels were significantly elevated(P<0.01).The BALF protein content and lung W/D weight ratio were significantly increased(P<0.01).Furthermore,the survival rate of rats was significantly reduced in the CLP group compared with the sham group.After the treatment,rats in the high-dose QWBD group had lower ALT(P<0.05),AST(P<0.01),Cr(P<0.05),BUN(P<0.01)levels,lower BALF protein content(P<0.05)and lower lung W/D weight ratio(P<0.01)than the CLP group.However,rats in the high-dose QWBD group had significantly better pathological changes in the lung,liver,and kidney compared to the sham group.After the treatment,the platelet level in the peripheral blood was elevated(P<0.05)and both activated partial thromboplastin time and prothrombin time were significantly shortened(P<0.01).The fibrinogen level was significantly increased(P<0.01).Finally,thrombin positive expression areas in the lung,liver,and kidney were significantly decreased in the high-dose QWBD group.Conclusion:QWBD can improve coagulation disorders caused by sepsis and has a protective effect on multiple organ injuries in rats.
文摘目的:通过观察清瘟败毒饮对脂多糖(LPS)诱导急性肺损伤(ALI)大鼠肺组织病理及核因子-κBp65(NF-κBp65)表达的影响,探讨清瘟败毒饮对ALI的治疗作用及干预机制。方法:选取健康雄性SD大鼠144只,随机分为6组,每组24只,分别为正常对照组,模型组,泼尼松组和清瘟败毒饮大、中、小剂量组。脂多糖溶液(2mg/kg)气管内滴注复制ALI大鼠模型,连续两天。各治疗组在第2次滴注完LPS溶液1h后,用生理盐水将相应的药物稀释至4mL灌胃,1天2次。各组分别于灌胃后24h、48h处死6只大鼠,于72h处死余下所有大鼠,并收集所需标本;通过镜下观察肺组织病理变化及采用W estern b lot方法检测肺组织中NF-κBp65的表达水平。结果:LPS致ALI大鼠肺组织肺泡结构破坏或实变,肺间质明显水肿,大量炎性细胞浸润,肺泡腔和肺间质内可见局灶性炎症细胞浸润和大量红细胞渗出,与同时相模型组比较,清瘟败毒饮大、中剂量组大鼠病理学炎症积分均降低非常显著(P<0.01),而小剂量组只有24h、48h有显著性降低或非常显著性降低(P<0.05或P<0.01);清瘟败毒饮各剂量治疗组中48h清瘟败毒饮大、中剂量组和72h各剂量组大鼠肺组织中NF-κBp65的表达均降低,与同时相模型组相比均有显著性差异或非常显著性差异(P<0.05或P<0.01)。结论:清瘟败毒饮能减轻LPS致ALI大鼠肺组织的损伤程度,在一定程度上减少炎症细胞在肺部积聚、浸润、渗出,起到比较明确的肺保护作用;清瘟败毒饮能有效减少LPS致ALI大鼠肺组织中NF-κBp65的表达,通过抑制NF-κB的活化和炎性细胞因子的生成,对炎症反应起到抑制作用;在LPS致ALI早期使用清瘟败毒饮,可有效减轻肺组织的损伤程度,减轻肺部的炎症反应。
