BACKGROUND Alzheimer’s disease(AD)is a neurodegenerative condition characterized by oxidative stress and neuroinflammation.Tanshinone ⅡA(Tan-ⅡA),a bioactive compound isolated from Salvia miltiorrhiza plants,has sho...BACKGROUND Alzheimer’s disease(AD)is a neurodegenerative condition characterized by oxidative stress and neuroinflammation.Tanshinone ⅡA(Tan-ⅡA),a bioactive compound isolated from Salvia miltiorrhiza plants,has shown potential neuroprotective effects;however,the mechanisms underlying such a function remain unclear.AIM To investigate potential Tan-ⅡA neuroprotective effects in AD and to elucidate their underlying mechanisms.METHODS Hematoxylin and eosin staining was utilized to analyze structural brain tissue morphology.To assess changes in oxidative stress and neuroinflammation,we performed enzyme-linked immunosorbent assay and western blotting.Additionally,the effect of Tan-ⅡA on AD cell models was evaluated in vitro using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.Genetic changes related to the long non-coding RNA(lncRNA)nuclear-enriched abundant transcript 1(NEAT1)/microRNA(miRNA,miR)-291a-3p/member RAS oncogene family Rab22a axis were assessed through reverse transcription quantitative polymerase chain reaction.RESULTS In vivo,Tan-ⅡA treatment improved neuronal morphology and attenuated oxidative stress and neuroinflammation in the brain tissue of AD mice.In vitro experiments showed that Tan-ⅡA dose-dependently ameliorated the amyloid-beta 1-42-induced reduction of neural stem cell viability,apoptosis,oxidative stress,and neuroinflammation.In this process,the lncRNA NEAT1-a potential therapeutic target-is highly expressed in AD mice and downregulated via Tan-ⅡA treatment.Mechanistically,NEAT1 promotes the transcription and translation of Rab22a via miR-291a-3p,which activates nuclear factor kappa-B(NF-κB)signaling,leading to activation of the pro-apoptotic B-cell lymphoma 2-associated X protein and inhibition of the anti-apoptotic B-cell lymphoma 2 protein,which exacerbates AD.Tan-ⅡA intervention effectively blocked this process by inhibiting the NEAT1/miR-291a-3p/Rab22a axis and NF-κB signaling.CONCLUSION This study demonstrates that Tan-ⅡA exerts neuroprotective effects in AD by modulating the NEAT1/miR-291a-3p/Rab22a/NF-κB signaling pathway,serving as a foundation for the development of innovative approaches for AD therapy.展开更多
ArcGIS Server可以构建Web应用、Web服务,ArcGIS Server的出现为网络地图服务提供了一个全新的途径。以ArcGIS Server10.0为平台研究网络地图服务系统的设计与实现,基于B/S三层混合模式,采用ArcGIS REST API和ArcGIS API For Flex,将Arc...ArcGIS Server可以构建Web应用、Web服务,ArcGIS Server的出现为网络地图服务提供了一个全新的途径。以ArcGIS Server10.0为平台研究网络地图服务系统的设计与实现,基于B/S三层混合模式,采用ArcGIS REST API和ArcGIS API For Flex,将ArcSDE作为空间数据引擎,SQLServer作为数据库进行空间数据管理,设计和实现一个具有基本地图操作功能、地图定位、查询、空间分析的地图网络发布系统,为地图的网络服务奠定基础。展开更多
物理实验数据的处理方法很多,大多数据处理能力有限,而一些采用Matlab开发的数据处理系统又不具备网络功能。通过具体实例研究了基于B/S结构、利用Matlab Web Server处理物理实验数据系统,解决了Web系统开发程序中数值计算能力较差,特...物理实验数据的处理方法很多,大多数据处理能力有限,而一些采用Matlab开发的数据处理系统又不具备网络功能。通过具体实例研究了基于B/S结构、利用Matlab Web Server处理物理实验数据系统,解决了Web系统开发程序中数值计算能力较差,特别是结果可视化难点问题,可以实现跨平台应用。用户只借助Web浏览器就可以在Internet上利用Matlab的强大计算功能实现实验数据的处理工作,可以利用其丰富的图形功能而不需安装Matlab软件。应用程序的安装和维护只需在服务器端进行,方便、安全,具有推广价值。展开更多
讨论了基于B/S模式的Matlab Web Server的网络应用开发原理,介绍了Matlab Web程序处理的一般流程和相关文件的详细配置方法,通过输入模块从HTML页面获取输入参数和通过输出模块生成包括输出数据和图片的HTML文件。最后利用MAT-LAB Web S...讨论了基于B/S模式的Matlab Web Server的网络应用开发原理,介绍了Matlab Web程序处理的一般流程和相关文件的详细配置方法,通过输入模块从HTML页面获取输入参数和通过输出模块生成包括输出数据和图片的HTML文件。最后利用MAT-LAB Web Server在湖南湘钢高线制造部开发出了基于B/S模式的品种钢建模与优化系统分析软件,使局域网里的用户通过网络,利用该系统可以对品种钢进行建模与优化,并借助于MATLAB强大的计算与图形展示功能,以图文并貌的方式返回到客户面前。展开更多
基金Supported by 2020 Guangxi Zhuang Autonomous Region Health Care Commission Self-Financing Research Projects,No.Z202000962023 Guangxi University Young and Middle-Aged Teachers’Basic Research Ability Improvement Project,No.2023KY0091+1 种基金National Natural Science Foundation of China,No.82260241the Natural Science Foundation of Guangxi Province,No.2015GXNSFAA139171 and No.2020GXNSFAA259053.
文摘BACKGROUND Alzheimer’s disease(AD)is a neurodegenerative condition characterized by oxidative stress and neuroinflammation.Tanshinone ⅡA(Tan-ⅡA),a bioactive compound isolated from Salvia miltiorrhiza plants,has shown potential neuroprotective effects;however,the mechanisms underlying such a function remain unclear.AIM To investigate potential Tan-ⅡA neuroprotective effects in AD and to elucidate their underlying mechanisms.METHODS Hematoxylin and eosin staining was utilized to analyze structural brain tissue morphology.To assess changes in oxidative stress and neuroinflammation,we performed enzyme-linked immunosorbent assay and western blotting.Additionally,the effect of Tan-ⅡA on AD cell models was evaluated in vitro using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.Genetic changes related to the long non-coding RNA(lncRNA)nuclear-enriched abundant transcript 1(NEAT1)/microRNA(miRNA,miR)-291a-3p/member RAS oncogene family Rab22a axis were assessed through reverse transcription quantitative polymerase chain reaction.RESULTS In vivo,Tan-ⅡA treatment improved neuronal morphology and attenuated oxidative stress and neuroinflammation in the brain tissue of AD mice.In vitro experiments showed that Tan-ⅡA dose-dependently ameliorated the amyloid-beta 1-42-induced reduction of neural stem cell viability,apoptosis,oxidative stress,and neuroinflammation.In this process,the lncRNA NEAT1-a potential therapeutic target-is highly expressed in AD mice and downregulated via Tan-ⅡA treatment.Mechanistically,NEAT1 promotes the transcription and translation of Rab22a via miR-291a-3p,which activates nuclear factor kappa-B(NF-κB)signaling,leading to activation of the pro-apoptotic B-cell lymphoma 2-associated X protein and inhibition of the anti-apoptotic B-cell lymphoma 2 protein,which exacerbates AD.Tan-ⅡA intervention effectively blocked this process by inhibiting the NEAT1/miR-291a-3p/Rab22a axis and NF-κB signaling.CONCLUSION This study demonstrates that Tan-ⅡA exerts neuroprotective effects in AD by modulating the NEAT1/miR-291a-3p/Rab22a/NF-κB signaling pathway,serving as a foundation for the development of innovative approaches for AD therapy.
文摘ArcGIS Server可以构建Web应用、Web服务,ArcGIS Server的出现为网络地图服务提供了一个全新的途径。以ArcGIS Server10.0为平台研究网络地图服务系统的设计与实现,基于B/S三层混合模式,采用ArcGIS REST API和ArcGIS API For Flex,将ArcSDE作为空间数据引擎,SQLServer作为数据库进行空间数据管理,设计和实现一个具有基本地图操作功能、地图定位、查询、空间分析的地图网络发布系统,为地图的网络服务奠定基础。
文摘物理实验数据的处理方法很多,大多数据处理能力有限,而一些采用Matlab开发的数据处理系统又不具备网络功能。通过具体实例研究了基于B/S结构、利用Matlab Web Server处理物理实验数据系统,解决了Web系统开发程序中数值计算能力较差,特别是结果可视化难点问题,可以实现跨平台应用。用户只借助Web浏览器就可以在Internet上利用Matlab的强大计算功能实现实验数据的处理工作,可以利用其丰富的图形功能而不需安装Matlab软件。应用程序的安装和维护只需在服务器端进行,方便、安全,具有推广价值。
文摘讨论了基于B/S模式的Matlab Web Server的网络应用开发原理,介绍了Matlab Web程序处理的一般流程和相关文件的详细配置方法,通过输入模块从HTML页面获取输入参数和通过输出模块生成包括输出数据和图片的HTML文件。最后利用MAT-LAB Web Server在湖南湘钢高线制造部开发出了基于B/S模式的品种钢建模与优化系统分析软件,使局域网里的用户通过网络,利用该系统可以对品种钢进行建模与优化,并借助于MATLAB强大的计算与图形展示功能,以图文并貌的方式返回到客户面前。
