Background:To explore the potential mechanism of action of the active ingredients of Smilacis Glabrae Rhizoma(SGR)in the treatment of migraine using network pharmacology and in vivo experiments.Methods:Through the sea...Background:To explore the potential mechanism of action of the active ingredients of Smilacis Glabrae Rhizoma(SGR)in the treatment of migraine using network pharmacology and in vivo experiments.Methods:Through the search of Traditional Chinese Medicines Systems Pharmacology Database and Analysis Platform,Genecards,Drugbank and other databases,we obtained active ingredients,targets of SGR and related disease targets of migraine,and took the intersection for protein-protein interactions analysis.After constructing the network diagram,network topology analysis was performed to derive the core targets and key active ingredients,and Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed.Finally,molecular docking was performed and validated by in vivo experiments.In vivo experiments,18 male BALB/c mice were selected,and the SGR group was fed with SGR drinking tablet concentrate,and nitroglycerin injection was used to construct a mouse model of migraine.Enzyme-linked immunosorbent assay test was used to detect the levels of TNF-α,IL-1β,IL-6,and AKT1 in plasma.Results:The results showed that the core targets of SGR for the treatment of migraine were TNF-α,IL-1β,IL-6,and AKT1.These core targets and key active ingredients had better binding ability.Compared with the blank group,the number of head scratching in the model group increased.Compared with the model group,there was a significant reduction of the number of head scratching in the SGR group.In comparison with the blank group,the protein level in the plasma in the model group was markedly higher.Compared with the model group,the protein level in the SGR group was significantly lower.Conclusion:SGR has the characteristics of improving migraine based on multi-targets,multi-components and multi-pathways,and the mechanism of action may be related to the inhibition of the release of inflammatory factors,neuron protection,and interference with apoptosis and other processes.展开更多
血液复苏是严重失血患者的关键急救措施。血液复苏液体的选用,经历了从全血到晶体液/胶体液、晶体液联合红细胞、等比例血液成分的演进过程。近20年来,采用低效价抗体O型全血(low titer group O whole blood,LTOWB)作为血液复苏的首选...血液复苏是严重失血患者的关键急救措施。血液复苏液体的选用,经历了从全血到晶体液/胶体液、晶体液联合红细胞、等比例血液成分的演进过程。近20年来,采用低效价抗体O型全血(low titer group O whole blood,LTOWB)作为血液复苏的首选应急通用桥接复苏液,已发展成为大趋势。我们对全血在严重失血患者的应用历史和现状做一介绍,并提出我国采用LTOWB作为首选应急通用血液的进一步论证和验证研究建议。展开更多
基金supported by Open Fund Project of Key Laboratory of Xin’an Medicine Ministry of Education(2020xayx09)Scientific Research Program of Chinese Society of Ethnomedicine(2020Z1066-410113).
文摘Background:To explore the potential mechanism of action of the active ingredients of Smilacis Glabrae Rhizoma(SGR)in the treatment of migraine using network pharmacology and in vivo experiments.Methods:Through the search of Traditional Chinese Medicines Systems Pharmacology Database and Analysis Platform,Genecards,Drugbank and other databases,we obtained active ingredients,targets of SGR and related disease targets of migraine,and took the intersection for protein-protein interactions analysis.After constructing the network diagram,network topology analysis was performed to derive the core targets and key active ingredients,and Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed.Finally,molecular docking was performed and validated by in vivo experiments.In vivo experiments,18 male BALB/c mice were selected,and the SGR group was fed with SGR drinking tablet concentrate,and nitroglycerin injection was used to construct a mouse model of migraine.Enzyme-linked immunosorbent assay test was used to detect the levels of TNF-α,IL-1β,IL-6,and AKT1 in plasma.Results:The results showed that the core targets of SGR for the treatment of migraine were TNF-α,IL-1β,IL-6,and AKT1.These core targets and key active ingredients had better binding ability.Compared with the blank group,the number of head scratching in the model group increased.Compared with the model group,there was a significant reduction of the number of head scratching in the SGR group.In comparison with the blank group,the protein level in the plasma in the model group was markedly higher.Compared with the model group,the protein level in the SGR group was significantly lower.Conclusion:SGR has the characteristics of improving migraine based on multi-targets,multi-components and multi-pathways,and the mechanism of action may be related to the inhibition of the release of inflammatory factors,neuron protection,and interference with apoptosis and other processes.
文摘血液复苏是严重失血患者的关键急救措施。血液复苏液体的选用,经历了从全血到晶体液/胶体液、晶体液联合红细胞、等比例血液成分的演进过程。近20年来,采用低效价抗体O型全血(low titer group O whole blood,LTOWB)作为血液复苏的首选应急通用桥接复苏液,已发展成为大趋势。我们对全血在严重失血患者的应用历史和现状做一介绍,并提出我国采用LTOWB作为首选应急通用血液的进一步论证和验证研究建议。
