Effective teaching is every teacher's teaching goal.How to reach the aim is to reflect on the teaching.The following document describes the development on how to reflect the teaching.The main purpose of this analy...Effective teaching is every teacher's teaching goal.How to reach the aim is to reflect on the teaching.The following document describes the development on how to reflect the teaching.The main purpose of this analysis is to show how effective and important the reflection on the teaching and to show how useful the reflection which can make the teacher change the way of teaching,encouraging the students to adopt a deep approach in their learning.展开更多
Human diploid fibroblasts (HDFs) undergo a limited number of cell divisions in culture. After certain population doublings, they reach a state of irreversible growth arrest known as replicative senescence. Senescent H...Human diploid fibroblasts (HDFs) undergo a limited number of cell divisions in culture. After certain population doublings, they reach a state of irreversible growth arrest known as replicative senescence. Senescent HDFs showed several molecular and cytological changes such as large flat morphology, expression of senescence-associated β-galactosidase (SA β-gal) activity and altered gene expression. Small interfering RNA (siRNA) has been demonstrated to be a potential research tool to analyse gene function and pathway. Expression of an appropriate housekeeping or reference gene can be used as a measurement of transfection efficiency in siRNA. Therefore this study was designed to determine the suitability of GAPDH expression as a measurement of transfection efficiency for p16INK4a gene silencing in HDFs aging model. GAPDH knockdown with an appropriate transfection reagent was measured by quantitative real time RT-PCR while cellular senescence was characterized based on morphological changes, expression of SA β-gal and p16INK4a expression levels. Our findings showed that GAPDH knockdown represents silencing efficiency and down regulation of p16INK4a in senescent transfected HDFs caused morphological alterations which results in the formation of spindle shaped fibroblasts. This study demonstrated the suitability of GAPDH expression as a measurement of transfection efficiency for p16INK4a gene silencing in HDFs aging model.展开更多
Di-p-methoxyphenyl-2, 2-dinitroethylene reacts with 1-benzyl-1, 4-dihydronico- tinamide (BNAH) in deaerated acetonitrile to give 1, 1-di-p-methoxyphenyl-2, 2-dinitroethane, while 1, 1-di-O-methoxyphenyl-2, 2-dinitroet...Di-p-methoxyphenyl-2, 2-dinitroethylene reacts with 1-benzyl-1, 4-dihydronico- tinamide (BNAH) in deaerated acetonitrile to give 1, 1-di-p-methoxyphenyl-2, 2-dinitroethane, while 1, 1-di-O-methoxyphenyl-2, 2-dinitroethylene fails to react with BNAH under the same conditions, which provides evidence for a concerted electron-hydrogen atom transfer mechanism.展开更多
In the big data era,robust solutions are obliged to be proposed to integrate and represent data from different formats and with different contents to assist the decision-making.Current cartographic and geographic info...In the big data era,robust solutions are obliged to be proposed to integrate and represent data from different formats and with different contents to assist the decision-making.Current cartographic and geographic information systems have limited capabilities for solving these problems.This paper describes an automatic and comprehensive system that conducts data fusion from all potentially related sources.In this system,a new Semantic Location Model(SemLM)is established to present the semantic concepts and location feature and demonstrate how locations are interrelated.In the SemLM,various types of location descriptors in different application scenarios can be analyzed and understood.Additionally,considering the challenges involved in data-intensive computation and visualization,this paper implements a Place-based Pan-Information System(P2S)as an innovative 4D system that dynamically associates and visualizes place-based information,using public security as the case study.展开更多
应用学习型组织(Learn ing O rgan ization)理论,将班级、代表队、体协等作为学习型组织进行整体设计;学习型公共体育教学模式由体育必修课、体育选修课、课外体育锻炼、竞技体育训练课组成“学习平台”;以体育课堂教学为中心,建立学习...应用学习型组织(Learn ing O rgan ization)理论,将班级、代表队、体协等作为学习型组织进行整体设计;学习型公共体育教学模式由体育必修课、体育选修课、课外体育锻炼、竞技体育训练课组成“学习平台”;以体育课堂教学为中心,建立学习型体育教学的导向机制;以培养学生主动学习为突破口,建立学生主动学习的运行机制;构建课内外一体化的学习大环境,建立实践与应用机制。展开更多
文摘Effective teaching is every teacher's teaching goal.How to reach the aim is to reflect on the teaching.The following document describes the development on how to reflect the teaching.The main purpose of this analysis is to show how effective and important the reflection on the teaching and to show how useful the reflection which can make the teacher change the way of teaching,encouraging the students to adopt a deep approach in their learning.
文摘Human diploid fibroblasts (HDFs) undergo a limited number of cell divisions in culture. After certain population doublings, they reach a state of irreversible growth arrest known as replicative senescence. Senescent HDFs showed several molecular and cytological changes such as large flat morphology, expression of senescence-associated β-galactosidase (SA β-gal) activity and altered gene expression. Small interfering RNA (siRNA) has been demonstrated to be a potential research tool to analyse gene function and pathway. Expression of an appropriate housekeeping or reference gene can be used as a measurement of transfection efficiency in siRNA. Therefore this study was designed to determine the suitability of GAPDH expression as a measurement of transfection efficiency for p16INK4a gene silencing in HDFs aging model. GAPDH knockdown with an appropriate transfection reagent was measured by quantitative real time RT-PCR while cellular senescence was characterized based on morphological changes, expression of SA β-gal and p16INK4a expression levels. Our findings showed that GAPDH knockdown represents silencing efficiency and down regulation of p16INK4a in senescent transfected HDFs caused morphological alterations which results in the formation of spindle shaped fibroblasts. This study demonstrated the suitability of GAPDH expression as a measurement of transfection efficiency for p16INK4a gene silencing in HDFs aging model.
文摘Di-p-methoxyphenyl-2, 2-dinitroethylene reacts with 1-benzyl-1, 4-dihydronico- tinamide (BNAH) in deaerated acetonitrile to give 1, 1-di-p-methoxyphenyl-2, 2-dinitroethane, while 1, 1-di-O-methoxyphenyl-2, 2-dinitroethylene fails to react with BNAH under the same conditions, which provides evidence for a concerted electron-hydrogen atom transfer mechanism.
基金This work is supported by the National Natural Science Foundation of China(grant number 41301517,41271401,41329001,41401524,1416509,and 1535031)the National Key Research and Development Program(grant number 2016YFB0502204)+3 种基金the Fundamental Research Funds for the Central Universities(grant number 413000010)National Science and Technology Support Plan,the National Key Technology R&D Program(grant number 2012BAH35B03)Guangxi Natural Science Foundation(grant number 2015GXNSFBA139191)Scientific Project of Guangxi Education Department(grant number KY2015YB189).
文摘In the big data era,robust solutions are obliged to be proposed to integrate and represent data from different formats and with different contents to assist the decision-making.Current cartographic and geographic information systems have limited capabilities for solving these problems.This paper describes an automatic and comprehensive system that conducts data fusion from all potentially related sources.In this system,a new Semantic Location Model(SemLM)is established to present the semantic concepts and location feature and demonstrate how locations are interrelated.In the SemLM,various types of location descriptors in different application scenarios can be analyzed and understood.Additionally,considering the challenges involved in data-intensive computation and visualization,this paper implements a Place-based Pan-Information System(P2S)as an innovative 4D system that dynamically associates and visualizes place-based information,using public security as the case study.
文摘应用学习型组织(Learn ing O rgan ization)理论,将班级、代表队、体协等作为学习型组织进行整体设计;学习型公共体育教学模式由体育必修课、体育选修课、课外体育锻炼、竞技体育训练课组成“学习平台”;以体育课堂教学为中心,建立学习型体育教学的导向机制;以培养学生主动学习为突破口,建立学生主动学习的运行机制;构建课内外一体化的学习大环境,建立实践与应用机制。
