Background:To confirm that the four constitutions of the Korean medicine is closely related to the intestinal flora,and explore the influence of specific intestinal microbes on these four populations.Methods:We collec...Background:To confirm that the four constitutions of the Korean medicine is closely related to the intestinal flora,and explore the influence of specific intestinal microbes on these four populations.Methods:We collect stool samples of four different physiques of the Taeeum,Soeum,Taeyang,and Shaoyang.Based on 16S rDNA amplicon sequencing,SPSS 18.0 statistical software and GraphPad Prism 8.0 mapping software,the body weight,body mass index,cholesterol and intestinal microcommunity composition of the four groups were analyzed and compared.Results:The results found that the body weight and body mass index of the Taeeum group were significantly higher than the Shaoyang group and the Soeum group,the body weight,cholesterol and body mass index of the Taeyang group were significantly higher than the Soeum Group.In the analysis of gut microbiota,the richness and diversity of intestinal microorganisms and the abundance ratio of posterior firmicutes to bacteroides were all different in the four populations,and there were 24 gut microbiota with significant differences(P<0.05).Among them,the abundance of[Eubacterium]coprostanoligenes group of Taeyang people,Taeeum people,Shaoyang people,and Soeum people were 3.71,9.62,9.36 and 9.33,respectively.Conclusion:[Eubacterium]coprostanoligenes group may be related to the differences in cholesterol content of the four populations.Similarly,the firmicutes/bacteroidetes abundance ratio may also be related to the differences in body weight,blood glucose and blood lipid among the four groups.展开更多
Objective: To analyze the distribution characteristics and clinical significance of SLCO1B1 and ApoE gene polymorphisms of the Li people in Hainan Island. Method: Selecting 502 high school students of the Li people fr...Objective: To analyze the distribution characteristics and clinical significance of SLCO1B1 and ApoE gene polymorphisms of the Li people in Hainan Island. Method: Selecting 502 high school students of the Li people from five cities and counties in Hainan Island (namely, Qiongzhong County, Dongfang City, Ledong County, Baoting County and Wuzhishan City) as research subjects in September, 2019;Applying PCR-fluorescence probe method to detect SLCO1B1 and ApoE genotypes of the Li people in Hainan Island, and statistically analyzing the distribution characteristics of gene frequency and the distribution differences in gene polymorphisms between different genders. Meanwhile, detecting the SLCO1B1 and ApoE gene of 527 people from the Han people in five regions mentioned before, so as to analyze the distribution differences of the SLCO1B1 and ApoE gene between the Han people and the Li people. Results: The frequency of each genotype of SLCO1B1 in the Li people in Hainan Island is: *1a/*1a 6.77%, *1a/*1b 27.09%, *1b/1b 41.63%, *1a/*5 0.00%, *1a/*15 4.78%, *1b/15 16.93%., *5/*5 0.00%, *5/*15 0.00%, *15/*15 2.79%;And that of ApoE is: e2/e2 0.40%, e2/e3 17.73%, e2/e4 2.39%, e3/e3 65.54%, e3/e4 12.55%, e4/e4 1.39%. There is no significant difference (P > 0.05) in other genotypes except weak metabolic genotypes (*5/*5, *5/*15 and *15/*15) between the Han and the Li peoples. Conclusion: The gene frequency of SLCO1B1 weak metabolic genotype is dramatically higher in the Li people of Hainan Island than that of the Han people in both Hainan Island and Central and South China, but there is no significant difference in ApoE gene frequency among them. Therefore, clinicians should adjust the dosage of statins and select the types of lipid-lowering drugs according to the differences in patients’ genotypes, and strengthen the management of patients with ApoE4 risk gene.展开更多
Objective:This study aimed to revise the Constitution in Chinese Medicine Questionnaire(Elderly Edition)(CCMQ-EE),as well as evaluate its reliability and validity.The objective was to provide a more accurate Chinese m...Objective:This study aimed to revise the Constitution in Chinese Medicine Questionnaire(Elderly Edition)(CCMQ-EE),as well as evaluate its reliability and validity.The objective was to provide a more accurate Chinese medicine constitution identification tool for geriatric health management and help further research on the relationship between geriatric diseases and constitution type,etc.Methods:The Delphi method and a cross-sectional survey of the elderly population were used.From April to October 2021,experts in different fields were invited to participate in the questionnaire,and items were revised through the experts'ratings and feedback.From December 2021 to February 2022,the revised CCMQ-EE was validated in the elderly population.Results:The coefficients of variation of rationality and importance scores decreased to less than 0.25 in the third round of Delphi.The Kendall coordination coefficients of rationality and importance in the third round were 0.15 and 0.17(P=.031 and P=.007,respectively).The revised CCMQ-EE was established with 34 items.The factor loadings of subscales were between 0.39 and 0.92,and the fitting effects of other subscales were acceptable,except for the inherited special constitution.The Cronbach alpha and test-retest reliability coefficients of each subscale ranged from 0.65 to 0.83,and 0.66 to 0.96,respectively.The composite reliability and average variance extraction were between 0.66 and 0.84,and between 0.32 and 0.57,respectively.The reliability and validity of the revised CCMQ-EE were acceptable or satisfactory.Conclusion:The revised CCMQ-EE was developed through a three-round Delphi process and internal team discussion.After the verification in the elderly in Beijing area,its measurement performance was good.The updated questionnaire can probably replace the initial version to better promote health management and help healthy aging by identifying the constitution type of the elderly.展开更多
In the history of Chinese-American people-to-people exchange,the implementation of the China-U.S.Fulbright Program is surely a bright facet which should not be ignored.This program has been cultivating talents in the ...In the history of Chinese-American people-to-people exchange,the implementation of the China-U.S.Fulbright Program is surely a bright facet which should not be ignored.This program has been cultivating talents in the fields of social sciences and humanities for the two countries,promoting Sino-U.S.communications in the domains of education,academia and culture,and propelling the diffusion and advance of both Chinese and American cultures.The Fulbright Program in China still has certain limitations waiting to be improved,and one obvious problem is its coverage in China.To solve this problem,more Chinese higher education institutions should be included in the China-U.S.Fulbright Program,and the result would be the generation of immeasurable value for the prosperity of Sino-American people-to-people exchange and therefore the construction of the new model of major country relations between China and the United States.展开更多
基金supported by the National Natural Science Foundation of China(No.81660834).
文摘Background:To confirm that the four constitutions of the Korean medicine is closely related to the intestinal flora,and explore the influence of specific intestinal microbes on these four populations.Methods:We collect stool samples of four different physiques of the Taeeum,Soeum,Taeyang,and Shaoyang.Based on 16S rDNA amplicon sequencing,SPSS 18.0 statistical software and GraphPad Prism 8.0 mapping software,the body weight,body mass index,cholesterol and intestinal microcommunity composition of the four groups were analyzed and compared.Results:The results found that the body weight and body mass index of the Taeeum group were significantly higher than the Shaoyang group and the Soeum group,the body weight,cholesterol and body mass index of the Taeyang group were significantly higher than the Soeum Group.In the analysis of gut microbiota,the richness and diversity of intestinal microorganisms and the abundance ratio of posterior firmicutes to bacteroides were all different in the four populations,and there were 24 gut microbiota with significant differences(P<0.05).Among them,the abundance of[Eubacterium]coprostanoligenes group of Taeyang people,Taeeum people,Shaoyang people,and Soeum people were 3.71,9.62,9.36 and 9.33,respectively.Conclusion:[Eubacterium]coprostanoligenes group may be related to the differences in cholesterol content of the four populations.Similarly,the firmicutes/bacteroidetes abundance ratio may also be related to the differences in body weight,blood glucose and blood lipid among the four groups.
文摘Objective: To analyze the distribution characteristics and clinical significance of SLCO1B1 and ApoE gene polymorphisms of the Li people in Hainan Island. Method: Selecting 502 high school students of the Li people from five cities and counties in Hainan Island (namely, Qiongzhong County, Dongfang City, Ledong County, Baoting County and Wuzhishan City) as research subjects in September, 2019;Applying PCR-fluorescence probe method to detect SLCO1B1 and ApoE genotypes of the Li people in Hainan Island, and statistically analyzing the distribution characteristics of gene frequency and the distribution differences in gene polymorphisms between different genders. Meanwhile, detecting the SLCO1B1 and ApoE gene of 527 people from the Han people in five regions mentioned before, so as to analyze the distribution differences of the SLCO1B1 and ApoE gene between the Han people and the Li people. Results: The frequency of each genotype of SLCO1B1 in the Li people in Hainan Island is: *1a/*1a 6.77%, *1a/*1b 27.09%, *1b/1b 41.63%, *1a/*5 0.00%, *1a/*15 4.78%, *1b/15 16.93%., *5/*5 0.00%, *5/*15 0.00%, *15/*15 2.79%;And that of ApoE is: e2/e2 0.40%, e2/e3 17.73%, e2/e4 2.39%, e3/e3 65.54%, e3/e4 12.55%, e4/e4 1.39%. There is no significant difference (P > 0.05) in other genotypes except weak metabolic genotypes (*5/*5, *5/*15 and *15/*15) between the Han and the Li peoples. Conclusion: The gene frequency of SLCO1B1 weak metabolic genotype is dramatically higher in the Li people of Hainan Island than that of the Han people in both Hainan Island and Central and South China, but there is no significant difference in ApoE gene frequency among them. Therefore, clinicians should adjust the dosage of statins and select the types of lipid-lowering drugs according to the differences in patients’ genotypes, and strengthen the management of patients with ApoE4 risk gene.
基金supported by the National Key Research and Development Program of China(2020YFC2003100&2020YFC2003102)Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine(ZYYCXTD-C-202001)。
文摘Objective:This study aimed to revise the Constitution in Chinese Medicine Questionnaire(Elderly Edition)(CCMQ-EE),as well as evaluate its reliability and validity.The objective was to provide a more accurate Chinese medicine constitution identification tool for geriatric health management and help further research on the relationship between geriatric diseases and constitution type,etc.Methods:The Delphi method and a cross-sectional survey of the elderly population were used.From April to October 2021,experts in different fields were invited to participate in the questionnaire,and items were revised through the experts'ratings and feedback.From December 2021 to February 2022,the revised CCMQ-EE was validated in the elderly population.Results:The coefficients of variation of rationality and importance scores decreased to less than 0.25 in the third round of Delphi.The Kendall coordination coefficients of rationality and importance in the third round were 0.15 and 0.17(P=.031 and P=.007,respectively).The revised CCMQ-EE was established with 34 items.The factor loadings of subscales were between 0.39 and 0.92,and the fitting effects of other subscales were acceptable,except for the inherited special constitution.The Cronbach alpha and test-retest reliability coefficients of each subscale ranged from 0.65 to 0.83,and 0.66 to 0.96,respectively.The composite reliability and average variance extraction were between 0.66 and 0.84,and between 0.32 and 0.57,respectively.The reliability and validity of the revised CCMQ-EE were acceptable or satisfactory.Conclusion:The revised CCMQ-EE was developed through a three-round Delphi process and internal team discussion.After the verification in the elderly in Beijing area,its measurement performance was good.The updated questionnaire can probably replace the initial version to better promote health management and help healthy aging by identifying the constitution type of the elderly.
文摘In the history of Chinese-American people-to-people exchange,the implementation of the China-U.S.Fulbright Program is surely a bright facet which should not be ignored.This program has been cultivating talents in the fields of social sciences and humanities for the two countries,promoting Sino-U.S.communications in the domains of education,academia and culture,and propelling the diffusion and advance of both Chinese and American cultures.The Fulbright Program in China still has certain limitations waiting to be improved,and one obvious problem is its coverage in China.To solve this problem,more Chinese higher education institutions should be included in the China-U.S.Fulbright Program,and the result would be the generation of immeasurable value for the prosperity of Sino-American people-to-people exchange and therefore the construction of the new model of major country relations between China and the United States.
