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Reading man皮瓣修复术治疗皮肤缺损的疗效观察
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作者 王鸣 严炜 +3 位作者 汤由之 朱喆辰 史京萍 马秀云 《临床皮肤科杂志》 CAS CSCD 北大核心 2024年第9期557-561,共5页
目的:探讨Reading man皮瓣修复术在全身不同部位皮肤缺损及其预防瘢痕增生的临床疗效。方法:回顾性分析2021年1月—2023年10月该科应用Reading man皮瓣修复术治疗全身不同部位皮肤软组织缺损的20例患者临床资料。结果:所有患者皮瓣均成... 目的:探讨Reading man皮瓣修复术在全身不同部位皮肤缺损及其预防瘢痕增生的临床疗效。方法:回顾性分析2021年1月—2023年10月该科应用Reading man皮瓣修复术治疗全身不同部位皮肤软组织缺损的20例患者临床资料。结果:所有患者皮瓣均成活。术后随访时间为3~24个月,所有皮瓣血运良好,色泽红润,质地柔软,外观适宜,局部皮肤张力较低,手术切口瘢痕增生不明显,切口周围软组织无明显形变,周围关节活动度正常。所有患者均恢复良好,疗效满意。结论:Reading man皮瓣特有的设计可以很好地解决全身多部位较大的皮肤软组织缺损,尤其在术中可以最大限度地减少正常皮肤的切除,术后外观、功能及皮肤瘢痕增生均有较满意的表现,且不存在自体皮移植带来的供皮区损伤。 展开更多
关键词 Reading man皮瓣 皮瓣血运 张力 瘢痕增生
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牛冠状病毒TaqMan荧光定量PCR检测方法的建立及应用
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作者 蒲鹏 李晨露 +2 位作者 张琪 吴发兴 许信刚 《动物医学进展》 北大核心 2024年第2期7-10,共4页
为建立牛冠状病毒TaqMan荧光定量PCR检测方法,根据GenBank收录的牛冠状病毒AKS-01株N基因序列(KU886219)保守区设计特异性引物和探针,构建重组质粒并进行反应条件优化、特异性试验、重复性试验以及敏感性试验,建立一种检测BCoV的TaqMan... 为建立牛冠状病毒TaqMan荧光定量PCR检测方法,根据GenBank收录的牛冠状病毒AKS-01株N基因序列(KU886219)保守区设计特异性引物和探针,构建重组质粒并进行反应条件优化、特异性试验、重复性试验以及敏感性试验,建立一种检测BCoV的TaqMan荧光定量PCR方法。结果显示,建立的牛冠状病毒TaqMan荧光定量PCR检测方法特异性、敏感性和重复性均良好。该方法BCoV重组质粒标准品在5.75×10^(7)~5.75×10^(3)copies/μL时与Ct值呈现良好线性关系,该方法对牛轮状病毒、牛传染性鼻气管炎病毒、牛病毒性腹泻病毒、牛副流感病毒3型均无交叉反应,特异性良好;该方法对BCoV重组质粒标准品最低检测限为5.75×10^(1)copies/μL;批内和批间重复性试验结果稳定,变异系数均小于2%。利用所建立的TaqMan荧光定量PCR方法对收集的132份样品进行检测,与常规PCR相比,两者符合率为96.21%,可为BCoV的临床检测和流行病学调查提供技术支持。 展开更多
关键词 牛冠状病毒 Taqman荧光定量PCR 检测方法
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大口黑鲈弹状病毒Taq Man荧光定量PCR检测方法的建立及初步应用 被引量:1
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作者 孔明慧 梁红茹 +5 位作者 李宁求 林强 牛银杰 罗霞 马宝福 付小哲 《中国预防兽医学报》 CAS CSCD 北大核心 2024年第4期379-384,共6页
为建立快速、敏感的大口黑鲈弹状病毒(MSRV)流行株Taq Man荧光定量PCR (qPCR)方法,本研究采用NCBI分析MSRV流行株N基因的保守序列,设计特异性引物和Taq Man探针,以构建的并经PCR和测序鉴定正确的重组质粒标准品p MD18-MSRV-N为模板,通... 为建立快速、敏感的大口黑鲈弹状病毒(MSRV)流行株Taq Man荧光定量PCR (qPCR)方法,本研究采用NCBI分析MSRV流行株N基因的保守序列,设计特异性引物和Taq Man探针,以构建的并经PCR和测序鉴定正确的重组质粒标准品p MD18-MSRV-N为模板,通过优化各反应条件初步建立MSRV的Taq Man q PCR检测方法。结果显示,建立的Taq Man q PCR方法标准曲线的R2为0.9906,质粒标准品在6.79×10^(8)拷贝/μL~6.79×10^(2)拷贝/μL范围内与Ct值均存在较好的线性关系。采用该方法检测MSRV及相关病毒,评估该方法的特异性,结果显示,该方法可以特异性检测MSRV,而草鱼出血病病毒、神经坏死病毒、鲤春病毒血症病毒、大口黑鲈双RNA病毒、鳜蛙虹彩病毒、传染性脾肾坏死病毒、石斑鱼虹彩病毒、大口黑鲈蛙虹彩病毒等8种常见鱼类病毒检测结果均为阴性;采用建立的方法检测不同浓度的质粒标准品(6.79×10^(8)拷贝/μL~6.79×10^(1)拷贝/μL),评估该方法的敏感性,结果显示,该方法的检测限为6.79×10^(2)拷贝/μL,比文献中的常规PCR方法敏感1 000倍;以不同批次或同一批次提取的不同浓度的质粒标准品作为模板,分别利用该方法检测,评估该方法的重复性,结果显示,该方法批内和批间重复性试验的变异系数均小于2%。采用该方法和文献中的常规PCR同时检测71份临床发病的大口黑鲈、鳜鱼、鲮鱼组织样品,结果显示阳性率为16.9%(12/71),阴性率为83.1%(59/71),且阳性样品均是从发病大口黑鲈的组织样品中检测到,而发病的鳜鱼、鲮鱼组织样品中均未检测到该病毒;常规PCR方法的阳性率为9.9%(7/71),阴性率为90.1%(64/71),二者的总符合率为93.0%。本研究建立的MSRV Taq Man q PCR方法特异性强、敏感性高、重复性和准确性均较好,可用于大口黑鲈临床样品的检测,为MSRV的快速检测提供了可行的技术手段。 展开更多
关键词 大口黑鲈弹状病毒 Taq man荧光定量PCR N基因
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鸡毒支原体 Taq Man实时荧光定量PCR检测方法的建立
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作者 孙宇 苗立中 +6 位作者 程立坤 赵家磊 赵修报 沈志强 王敬茹 李书光 余燕 《动物医学进展》 北大核心 2024年第4期64-68,共5页
为快速检测鸡毒支原体,根据鸡毒支原体的保守基因设计特异性引物和Taq Man探针,建立鸡毒支原体Taq Man实时荧光定量PCR方法并分析鸡毒支原体培养过程中颜色单位改变法(color change unit,CCU)和荧光定量PCR检测的相关性。结果显示,标准... 为快速检测鸡毒支原体,根据鸡毒支原体的保守基因设计特异性引物和Taq Man探针,建立鸡毒支原体Taq Man实时荧光定量PCR方法并分析鸡毒支原体培养过程中颜色单位改变法(color change unit,CCU)和荧光定量PCR检测的相关性。结果显示,标准曲线y=-3.646x+44.208,相关系数R^(2)=0.998,最低检测限度为1 copy/μL;与其他菌株等均无交叉反应;组内和组间变异系数均小于3%,表明该方法具有良好的稳定性和可重复性。用建立的实时荧光定量PCR检测方法对26份临床样品进行检测,该方法较普通PCR方法检出率更高。建立的荧光定量PCR与CCU显示鸡毒支原体在对数生长期时,两者具有一定的对应关系。表明建立了鸡毒支原体Taq Man探针实时荧光定量PCR检测方法,可实现对鸡毒支原体的快速检测,为鸡毒支原体感染的防控提供技术基础。 展开更多
关键词 鸡毒支原体 Taq man探针 荧光定量PCR
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牛支原体和丝状支原体丝状亚种双重TaqMan荧光定量PCR检测方法的建立与应用 被引量:3
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作者 马芷忻 武琪 +2 位作者 刘桐 辛九庆 徐青元 《中国预防兽医学报》 CAS CSCD 北大核心 2024年第3期262-268,共7页
牛传染性胸膜肺炎和牛支原体病均为重要的牛传染病,两者均可致牛出现呼吸系统症状,临床上难以区分。为建立鉴别诊断牛传染性胸膜肺炎和牛支原体病的双重Taq Man荧光定量PCR检测方法,本研究根据这两种病的病原丝状支原体丝状亚种和牛支... 牛传染性胸膜肺炎和牛支原体病均为重要的牛传染病,两者均可致牛出现呼吸系统症状,临床上难以区分。为建立鉴别诊断牛传染性胸膜肺炎和牛支原体病的双重Taq Man荧光定量PCR检测方法,本研究根据这两种病的病原丝状支原体丝状亚种和牛支原体的基因组保守区域(丝状支原体丝状亚种nt826-nt1742,牛支原体nt189-nt618)分别设计引物与探针,通过优化反应体系与反应条件,建立同时检测这两种病原的Taq Man荧光定量PCR检测方法。特异性试验结果显示,该方法仅对丝状支原体丝状亚种模拟样品和牛支原体检测结果为阳性,而巴氏杆菌、牛传染性鼻气管炎病毒、无乳支原体、山羊支原体山羊肺炎亚种、丝状支原体山羊亚种、关节炎支原体Leachii株检测结果均为阴性,特异性较强。分别以1.0×10~7拷贝/μL~1.0×10~1拷贝/μL的p EASY-Mmm和p EASY-Mb质粒标准品为模板,进行敏感性试验,结果显示,该方法对丝状支原体丝状亚种和牛支原体重组质粒标准品的检测限均为1.0×10~1拷贝/μL,敏感性较高。对不同浓度质粒标准品混合物的重复性试验结果显示,组内与组间重复性试验变异系数均小于2.5%,重复性较好。利用该方法对112份临床样品(104份鼻拭子、8份肺组织样品)检测,结果显示,丝状支原体丝状亚种检测结果和已发表PCR方法检测结果一致,均为阴性,而本实验建立的方法检测出4份牛支原体阳性样品,且经测序证实检出样品为真实阳性样品,而已发表的多重PCR方法检测该病原结果均为阴性。本研究建立了能够同时检测丝状支原体丝状亚种和牛支原体的双重Taq Man荧光定量PCR方法,其特异性强、敏感性高、重复性好,可用于各种临床样品的检测,为这两种病原的快速检测和流行病学调查提供了技术手段。 展开更多
关键词 牛传染性胸膜肺炎 牛支原体 Taqman荧光定量PCR
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羊贝氏柯克斯体TaqMan荧光定量PCR检测方法的建立及应用
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作者 张锐铮 于皓同 +3 位作者 王凯茸 张琪 张淑霞 许信刚 《动物医学进展》 北大核心 2024年第7期6-10,共5页
为建立检测贝氏柯克斯体(Coxiella burnetii)实时荧光定量PCR方法,根据GenBank上收录的C.burnetii RSA439株com1基因序列(CP040059.1)保守区设计特异性引物和探针,并构建重组质粒及优化反应条件,以期建立一种检测贝氏柯克斯体的Taq Man... 为建立检测贝氏柯克斯体(Coxiella burnetii)实时荧光定量PCR方法,根据GenBank上收录的C.burnetii RSA439株com1基因序列(CP040059.1)保守区设计特异性引物和探针,并构建重组质粒及优化反应条件,以期建立一种检测贝氏柯克斯体的Taq Man荧光定量PCR方法。结果显示,该方法所建立标准曲线线性关系良好;敏感性试验结果显示,最低检出限为2.45×10^(2) copies/μL,与PCR最低检出限为2.45×10^(4) copies/μL相比较,灵敏度提高了100倍;对羊支原体、羊流产衣原体、羊布鲁氏菌等均无交叉反应,批内和批间重复性试验结果稳定,变异系数均小于3%,说明特异性、重复性好;利用该方法对收集的126份样品进行检测,结果显示样品阳性率为9.52%,常规PCR检测阳性率为7.14%,符合率为90.47%。该方法可为贝氏柯克斯体临床上快速检测及防控提供技术支持。 展开更多
关键词 贝氏柯克斯 Taq man荧光定量PCR 检测方法
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猪肺炎支原体和猪鼻支原体TaqMan双重荧光PCR检测方法的建立及应用 被引量:1
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作者 周颖 赵硕 +10 位作者 覃国喜 梁龙华 肖婷 陈忠伟 卢冰霞 秦毅斌 林昌华 张胜斌 段群棚 胡庭俊 何颖 《动物医学进展》 北大核心 2024年第2期11-16,共6页
为建立一种可快捷鉴别检测猪肺炎支原体(Mhp)和猪鼻支原体(Mhr)的方法,针对Mhp的183基因和Mhr的p37基因保守片段,分别设计合成引物及TaqMan探针,优化反应条件,建立一种可同时检测Mhp和Mhr的TaqMan双重荧光PCR方法,并评价其特异性、敏感... 为建立一种可快捷鉴别检测猪肺炎支原体(Mhp)和猪鼻支原体(Mhr)的方法,针对Mhp的183基因和Mhr的p37基因保守片段,分别设计合成引物及TaqMan探针,优化反应条件,建立一种可同时检测Mhp和Mhr的TaqMan双重荧光PCR方法,并评价其特异性、敏感性和重复性。结果表明,该方法特异性强,检测其他常见猪呼吸系统病原不发生交叉反应;对标准品pMD18-T-Mhp-183、pMD18-T-Mhr-P37的最低检测限分别达45.2 copies/μL和29.7 copies/μL,比普通PCR检测灵敏度提高10^(3)~10^(4)倍;该方法检测结果的批内与批间变异系数均小于2%。用该方法检测145份广西自治区内临床样品,从中检出82份Mhp和9份Mhr阳性样品。该方法可用于临床样品快速检测及实验室Mhp和Mhr的鉴定,可为Mhp和Mhr在猪群中的早期监测提供技术支持。 展开更多
关键词 猪肺炎支原体 猪鼻支原体 Taqman荧光定量PCR
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circMAN1A2调节miR-212-3p/DDIT4轴对胃癌细胞增殖、侵袭和迁移的影响
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作者 曹博威 贾腾飞 +2 位作者 宋钉町 赵昱清 张文斌 《胃肠病学和肝病学杂志》 CAS 2024年第7期868-874,共7页
目的探究circMAN1A2靶向miR-212-3p/DNA损伤诱导转录子4(NDA damage-inducible transcript 4,DDIT4)轴对胃癌细胞增殖、侵袭和迁移的影响。方法qRT-PCR法检测胃癌组织和细胞中circMAN1A2、miR-212-3p表达水平。荧光素酶检测circMAN1A2与... 目的探究circMAN1A2靶向miR-212-3p/DNA损伤诱导转录子4(NDA damage-inducible transcript 4,DDIT4)轴对胃癌细胞增殖、侵袭和迁移的影响。方法qRT-PCR法检测胃癌组织和细胞中circMAN1A2、miR-212-3p表达水平。荧光素酶检测circMAN1A2与miR-212-3p、DDIT4和miR-212-3p的靶向关系。在胃癌细胞SGC-7901、HGC-27中转染si-NC、si-circMAN1A2、si-circMAN1A2+anti-NC、si-circMAN1A2+anti-miR-212-3p、miR-NC、miR-212-3p mimics,将未转染的SGC-7901、HGC-27细胞设为对照。平板克隆实验检测细胞增殖;细胞划痕及Transwell实验分别检测细胞迁移、侵袭能力。Western blotting检测增殖、迁移及侵袭相关蛋白表达。结果在胃癌组织及细胞中,circMAN1A2高表达,miR-212-3p低表达;下调circMAN1A2可以靶向上调miR-212-3p表达,且下调DDIT4表达,进而抑制胃癌细胞增殖、侵袭和迁移能力。抑制miR-212-3p表达可部分逆转抑制circMAN1A2表达对胃癌细胞恶性增殖的抑制作用。结论在胃癌细胞中抑制circMAN1A2表达可抑制胃癌细胞增殖、迁移与侵袭,其与调节miR-212-3p/DDIT4信号轴有关。 展开更多
关键词 环状RNA man1A2 微小RNA-212-3p DNA损伤诱导转录子4 胃癌 增殖 侵袭 迁移
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猪细小病毒TaqMan荧光定量PCR检测方法的建立及应用
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作者 杨奕 吴发兴 +4 位作者 康京丽 孙洪涛 王志亮 许信刚 张琪 《动物医学进展》 北大核心 2024年第2期75-79,共5页
旨在建立一种猪细小病毒(PPV)快速准确PCR检测方法,根据PPV VP2基因的保守序列设计并合成1对特异性引物和1条特异性探针,建立了可检测PPV的TaqMan荧光定量PCR检测方法。建立的检测方法敏感性高,最低可检测8.76×10^(1)copies/μL的P... 旨在建立一种猪细小病毒(PPV)快速准确PCR检测方法,根据PPV VP2基因的保守序列设计并合成1对特异性引物和1条特异性探针,建立了可检测PPV的TaqMan荧光定量PCR检测方法。建立的检测方法敏感性高,最低可检测8.76×10^(1)copies/μL的PPV VP2基因标准品;特异性试验结果显示,该方法检测猪流行性腹泻病毒、猪圆环病毒2型、伪狂犬病病毒、猪繁殖与呼吸综合征病毒均无交叉反应;重复性试验组内与组间变异系数均低于2%,重复性良好。用该方法检测102份临床样本,检出阳性样本16份,阳性检出率为15.69%,将检测出的阳性样本进行PCR扩增并测序,证实检测样品中确实存在PPV。该方法敏感性高、特异性强,适用于PPV的定量检测及猪细小病毒病的流行病学调查。 展开更多
关键词 猪细小病毒 VP2 TAQman探针 荧光定量PCR
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虾十足目虹彩病毒1、肝肠胞虫及传染性早熟病毒多重Taq Man荧光定量PCR检测方法的建立与应用
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作者 徐晔 周毅 +4 位作者 王娜 窦维伟 王静 王凤芝 段宏安 《中国预防兽医学报》 CAS CSCD 北大核心 2024年第8期825-831,共7页
为建立检测十足目虹彩病毒1(DIV1)、肝肠胞虫(EHP)、传染性早熟病毒(IPV)的多重Taq Man荧光定量PCR检测方法,本研究根据DIV1 MCP基因、EHP SSU基因与IPV polyprotein A基因设计特异性引物和Taq Man探针,经各反应条件的优化建立了同时检... 为建立检测十足目虹彩病毒1(DIV1)、肝肠胞虫(EHP)、传染性早熟病毒(IPV)的多重Taq Man荧光定量PCR检测方法,本研究根据DIV1 MCP基因、EHP SSU基因与IPV polyprotein A基因设计特异性引物和Taq Man探针,经各反应条件的优化建立了同时检测DIV1、EHP及IPV的多重Taq Man荧光定量PCR方法。利用该方法检测DIV1、EHP、IPV、白斑综合征病毒、致急性肝胰腺坏死弧菌、传染性皮下及造血器官坏死病毒、虾偷死病毒、传染性肌肉坏死病毒、桃拉病毒、黄头病毒1型等主要虾类病毒,结果显示,该方法仅能扩增出DIV1、EHP和IPV,与其他主要虾类病原均无交叉反应,特异性较强。对10倍倍比稀释后等浓度等体积混合的DIV1、EHP和IPV质粒标准品混合物的检测结果显示,该方法对3种病原的质粒标准品的检测限均为1.67×10^(1)拷贝/μL,各病毒单一荧光定量PCR方法的检测限均为1.67×10~0拷贝/μL,多重Taq Man荧光定量PCR方法的灵敏度虽略低于单一荧光定量PCR方法,但是可以同时检测3种病原,检测效率高。选取3个浓度的pDIV1、pEHP、pIPV(5×10^(5)拷贝/μL、5×10^(4)拷贝/μL、5×10^(3)拷贝/μL)等浓度等体积混合的质粒标准品为模板,进行组内和组间重复性试验,结果显示组内与组间重复性试验的变异系数均小于1%,该方法重复性较好。利用该方法与DIV1、EHP、IPV单一荧光定量PCR方法同时检测105份虾苗和亲虾样品,结果显示,本研究建立的多重Taq Man荧光定量PCR方法对DIV1的检出率为21.9%(23/105),对EHP的检出率为14.3%(15/105),对IPV的检出率为45.7%(48/105),3种病原混合感染率为1.9%(2/105),与DIV1、EHP、IPV各单一荧光定量PCR方法的检测结果均一致,阳性样品的符合率达100%。本研究建立的DIV1、EHP、IPV多重Taq Man荧光定量PCR方法为临床同时检测该3种病原提供了特异、敏感、高效的技术手段。 展开更多
关键词 虾十足目虹彩病毒1 虾肝肠胞虫 虾传染性早熟病毒 多重Taq man荧光定量PCR
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TaqMan探针双重实时荧光定量PCR法同步检测兰花中的建兰花叶病毒和齿兰环斑病毒
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作者 孙爱青 王丽花 +3 位作者 张艺萍 杨秀梅 许凤 苏艳 《植物保护》 CAS CSCD 北大核心 2024年第2期219-227,239,共10页
为建立一种同步定量检测建兰花叶病毒(cymbidium mosaic virus,CymMV)和齿兰环斑病毒(odontoglossum ringspot virus,ORSV)的高效检测方法,本研究根据CymMV和ORSV CP基因高度保守区分别设计引物和探针并筛选,获得156 bp和148 bp的靶标... 为建立一种同步定量检测建兰花叶病毒(cymbidium mosaic virus,CymMV)和齿兰环斑病毒(odontoglossum ringspot virus,ORSV)的高效检测方法,本研究根据CymMV和ORSV CP基因高度保守区分别设计引物和探针并筛选,获得156 bp和148 bp的靶标序列及对应的最优特异性引物探针组合,建立了基于TaqMan探针的双重实时荧光定量PCR方法。该方法最低检出限为1拷贝或6.2×10^(-3)fg,最低稳定检出限为10拷贝或6.2×10^(-2)fg,是RT-PCR的10~100倍;构建的标准曲线线性关系良好,扩增效率分别为97.7%和100.2%,相关系数R2分别为1.000和0.999;对其他5种常见病毒均无扩增曲线,检测特异性强;批组内与批组间重复性试验Ct值变异系数≤0.60%,重复性和稳定性好。利用该方法和基因芯片法分别对4个种属的66个兰花样品进行方法验证,该方法相较于基因芯片法检出率提高了53.85%(CymMV)和162.5%(ORSV)。综上所述,本方法可同时高通量检测CymMV和ORSV 2种病毒靶基因,结果可靠,应用前景广阔,可为开展病毒精准鉴定、科学防控以及从源头遏制病毒传播提供技术支撑。 展开更多
关键词 建兰花叶病毒 齿兰环斑病毒 TAQman探针 RT-QPCR 兰花
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Impact of cattle density on the structure and natural regeneration of a turkey oak stand on an agrosilvopastoral farm in central Italy
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作者 Alessandra Pacini Francesco Pelleri +4 位作者 Francesco Marini Alberto Maltoni Barbara Mariotti Gianluigi Mazza Maria Chiara Manetti 《Journal of Forestry Research》 SCIE EI CAS CSCD 2024年第1期168-182,共15页
On an agrosilvopastoral farm in central Italy where Maremmana cattle graze in Turkey oak forests,we evaluated the impact of different livestock densities on stand structure,tree diversity and natural regeneration in f... On an agrosilvopastoral farm in central Italy where Maremmana cattle graze in Turkey oak forests,we evaluated the impact of different livestock densities on stand structure,tree diversity and natural regeneration in four types of grazed areas based on the grazing regime adopted:calf-grazed,high-intensity-grazed,low-intensity-grazed,ungrazed control.For each area,we set up three permanent circular plots(radius of 15 m)to survey the structural and dasometric characteristics of the overstorey,understorey,and regeneration layer.The results showed that grazing negatively affected the complexity of the forest structure and its potential to regenerate and maintain a high level of biodiversity.The differences in stand structure observed between the grazing areas were closely related to livestock density.The most sensitive components of the system were the understorey and the regeneration layers.Contrarily,the current grazing management did not affect the dominant tree structure or its composition.Our findings identified medium-term monitoring and regeneration management as the two significant aspects to consider when assessing sustainable livestock.New forests can be established by excluding graz-ing for about 20–25 years. 展开更多
关键词 AGROFORESTRY stand structure Regeneration Tree biodiversity Grazing intensity
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Soil properties and not plant factors affect both abundant and rare microbial taxa after thinning in a mixed stand of Cunninghamia lanceolata and Sassafras tzumu
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作者 Wanxia Peng Tongqing Song +5 位作者 Hu Du Feng Wang Fuping Zeng Yang Wang Shulin Chen Yinzhu Chen 《Journal of Forestry Research》 SCIE EI CAS CSCD 2024年第2期81-94,共14页
Thinning is an effective management step for sustainable forest development,yet less attention is paid to the restoration of soil microbiota after thinning.In this study,both abundant and rare soil microbial communiti... Thinning is an effective management step for sustainable forest development,yet less attention is paid to the restoration of soil microbiota after thinning.In this study,both abundant and rare soil microbial communities(i.e.,bacterial,fungal),were evaluated under various thinning treatments in a mixed stand of Cunninghamia lanceolata and Sassafras tzumu using Mi Seq sequencing.Thinning did not significantly change either abundant or rare bacterial and fungal community composition,but affected their alpha diversity.The Shannon–Wiener indexes of rare fungal taxa under medium thinning were significantly lower than in the light thinning(P<0.05 level).Xanthobacteraceae dominated the abundant bacterial taxa,and Saitozyma and Mortierlla the abundant fungal taxa.The most common rare bacterial taxa varied;there was no prevalent rare fungal taxa under different thinnings.In addition,soil available nitrogen,total phosphorus,and p H had significant effects on rare bacterial taxa.Nutrients,especially available phosphorus,but not nitrogen,affected abundant and rare soil fungi.The results indicate that soil properties rather than plant factors affect abundant and rare microbial communities in soils of mixed stands.Thinning,through mediating soil properties,influences both abundant and rare bacterial and fungal communities in the mixed C.lanceolata and S.tzumu stand. 展开更多
关键词 Diversity Abundant taxa Rare taxa THINNING Mixed stand
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Simulation of Haloxylon Ammodendron Stand Basic Diameter Structure Based on Fuzzy Distribution Function
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作者 Shaohua Wang Chuanqiang Liu Ting Yang 《Agricultural Sciences》 2024年第1期132-145,共14页
Based on the investigation data of 12 Haloxylon ammodendron plots in the south edge of Gurbantunggut Desert, Fuzzy distribution was introduced into the study of Haloxylon ammodendron base diameter structure fitting ac... Based on the investigation data of 12 Haloxylon ammodendron plots in the south edge of Gurbantunggut Desert, Fuzzy distribution was introduced into the study of Haloxylon ammodendron base diameter structure fitting according to the consistency between the characteristics of Fuzzy distribution function and the distribution series of cumulative percentage of stand base diameter, and the fitting precision and effect of Fuzzy distribution function were discussed. The root mean square error RMSE and determination coefficient R<sup>2</sup> values showed that Fuzzy-Γ<sub>1</sub>, Fuzzy-Γ<sub>2</sub>, Fuzzy-Γ<sub>3</sub>, Fuzzy-Γ<sub>4</sub> had good fitting performance, among which Fuzzy-Γ<sub>1</sub> had relatively high fitting precision, and its parameters were closely related to stand age and density, Fuzzy-Γ<sub>2</sub> distribution function was the second, and Fuzzy-Γ<sub>4</sub> distribution function had the worst fitting effect. By introducing a parameter c from the similarity of four distribution function formulas, a generalized Fuzzy distribution function Fuzzy-Γ<sub>5</sub> is obtained. This function shows the highest fitting accuracy. Most of the values of parameter c are near 1 or 2, which shows that the diameter distribution is mainly approximate to Fuzzy-Γ<sub>1</sub> and Fuzzy-Γ<sub>2</sub>. 展开更多
关键词 Fuzzy Distribution Function Haloxylon Ammodendron Base Diameter Distribution stand Factor
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The effects of data aggregation on long-term projections of forest stands development
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作者 Kobra Maleki Rasmus Astrup +2 位作者 Nicolas Cattaneo Wilson Lara Henao Clara Anton-Fernandez 《Forest Ecosystems》 SCIE CSCD 2024年第3期381-389,共9页
Forest management planning often relies on Airborne Laser Scanning(ALS)-based Forest Management Inventories(FMIs)for sustainable and efficient decision-making.Employing the area-based(ABA)approach,these inventories es... Forest management planning often relies on Airborne Laser Scanning(ALS)-based Forest Management Inventories(FMIs)for sustainable and efficient decision-making.Employing the area-based(ABA)approach,these inventories estimate forest characteristics for grid cell areas(pixels),which are then usually summarized at the stand level.Using the ALS-based high-resolution Norwegian Forest Resource Maps(16 m×16 m pixel resolution)alongside with stand-level growth and yield models,this study explores the impact of three levels of pixel aggregation(standlevel,stand-level with species strata,and pixel-level)on projected stand development.The results indicate significant differences in the projected outputs based on the aggregation level.Notably,the most substantial difference in estimated volume occurred between stand-level and pixel-level aggregation,ranging from-301 to+253 m^(3)·ha^(-1)for single stands.The differences were,on average,higher for broadleaves than for spruce and pine dominated stands,and for mixed stands and stands with higher variability than for pure and homogenous stands.In conclusion,this research underscores the critical role of input data resolution in forest planning and management,emphasizing the need for improved data collection practices to ensure sustainable forest management. 展开更多
关键词 Growth and yield models Dominant species Norway spruce Scots pine BROADLEAVES Forest resource map stand variability
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Fast solution to the free return orbit's reachable domain of the manned lunar mission by deep neural network
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作者 YANG Luyi LI Haiyang +1 位作者 ZHANG Jin ZHU Yuehe 《Journal of Systems Engineering and Electronics》 SCIE CSCD 2024年第2期495-508,共14页
It is important to calculate the reachable domain(RD)of the manned lunar mission to evaluate whether a lunar landing site could be reached by the spacecraft. In this paper, the RD of free return orbits is quickly eval... It is important to calculate the reachable domain(RD)of the manned lunar mission to evaluate whether a lunar landing site could be reached by the spacecraft. In this paper, the RD of free return orbits is quickly evaluated and calculated via the classification and regression neural networks. An efficient databasegeneration method is developed for obtaining eight types of free return orbits and then the RD is defined by the orbit’s inclination and right ascension of ascending node(RAAN) at the perilune. A classify neural network and a regression network are trained respectively. The former is built for classifying the type of the RD, and the latter is built for calculating the inclination and RAAN of the RD. The simulation results show that two neural networks are well trained. The classification model has an accuracy of more than 99% and the mean square error of the regression model is less than 0.01°on the test set. Moreover, a serial strategy is proposed to combine the two surrogate models and a recognition tool is built to evaluate whether a lunar site could be reached. The proposed deep learning method shows the superiority in computation efficiency compared with the traditional double two-body model. 展开更多
关键词 manned lunar mission free return orbit reachable domain(RD) deep neural network computation efficiency
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DOPO-MAN改性大豆油基水性聚氨酯丙烯酸酯的制备
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作者 秦良雨 彭丽 王念贵 《胶体与聚合物》 CAS 2024年第2期78-81,共4页
本文以9,10-二氢-9-氧杂-10-磷杂菲-10-氧化物(DOPO)、马来酸酐(MA)和二乙醇胺(DEA)为原料制备出DOPO-MAN,以其为改性剂制备出可UV-LED固化的DOPO-MAN改性大豆油基水性聚氨酯丙烯酸酯乳液。将乳液与光引发剂(TPO)混合均匀后经UV-LED光固... 本文以9,10-二氢-9-氧杂-10-磷杂菲-10-氧化物(DOPO)、马来酸酐(MA)和二乙醇胺(DEA)为原料制备出DOPO-MAN,以其为改性剂制备出可UV-LED固化的DOPO-MAN改性大豆油基水性聚氨酯丙烯酸酯乳液。将乳液与光引发剂(TPO)混合均匀后经UV-LED光固化,制成固化膜。实验结果表明,当DOPO-MAN的含量为20%时,固化膜的极限氧指数为24.3,UL-94测试也达到了V-1级别。 展开更多
关键词 水性聚氨酯 大豆油 DOPO-man 改性
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Effects of Standing Time during Pretreatment on the Nitrite Concentration Detected by Spectrophotometric Method
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作者 Yingfei Zeng Juan Hu +2 位作者 Xianglong Bian Qianfeng Xia Tingwei Hu 《Journal of Materials Science and Chemical Engineering》 2024年第2期73-83,共11页
Food safety problems caused by excessive nitrite addition have been frequently reported and the detection of nitrite in food is particularly important. The standing time during the pretreatment of primary sample has a... Food safety problems caused by excessive nitrite addition have been frequently reported and the detection of nitrite in food is particularly important. The standing time during the pretreatment of primary sample has a great influence on the concentration of nitrite tested by spectrophotometric method. In this context, three kinds of food samples are prepared, including canned mustard, canned fish and home-made pickled water. A series of standing times are placed during the sample pretreatments and the corresponding nitrite contents in these samples are detected by spectrophotometric method based on N-ethylenediamine dihydrochloride. This study aims to find out a reasonable standing time during the pretreatment of food sample, providing influence factor for precise detection of nitrite. 展开更多
关键词 standing Time Spectrophotometric Method Nitrite Detection
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Existence and Stability of Standing Waves for the Nonlinear Schrödinger Equation with Combined Nonlinearities and a Partial Harmonic Potential
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作者 Wei Wang 《Journal of Applied Mathematics and Physics》 2024年第5期1606-1615,共10页
In this paper, we study the existence of standing waves for the nonlinear Schrödinger equation with combined power-type nonlinearities and a partial harmonic potential. In the L<sup>2</sup>-supercriti... In this paper, we study the existence of standing waves for the nonlinear Schrödinger equation with combined power-type nonlinearities and a partial harmonic potential. In the L<sup>2</sup>-supercritical case, we obtain the existence and stability of standing waves. Our results are complements to the results of Carles and Il’yasov’s artical, where orbital stability of standing waves have been studied for the 2D Schrödinger equation with combined nonlinearities and harmonic potential. 展开更多
关键词 Nonlinear Schrödinger Equation Orbital Stability standing Waves
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Runoff Characteristics of Different Stands in Dongjiang Lake Reservoir Area
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作者 Zhangquan ZENG Yaqin XIAO +5 位作者 Dewei XIAO Qinxiang SHAN Ni ZHANG Yang SONG Canming ZHANG Rui YANG 《Meteorological and Environmental Research》 2024年第4期36-38,共3页
Different forest stands in the Dongjiang Lake Reservoir area of Zixing were selected as the research objects to study the characteristics of runoff generation in different forest stands.The results showed that there w... Different forest stands in the Dongjiang Lake Reservoir area of Zixing were selected as the research objects to study the characteristics of runoff generation in different forest stands.The results showed that there was no significant difference in annual runoff among M3,M1,and M5,and no significant difference between each forest stand and the control.The order was M3(22.75 mm)>M1(21.77 mm)>M5(20.14 mm).Forest vegetation generates less runoff through vegetation restoration compared to the control,indicating that forest vegetation reconstruction and restoration are beneficial for soil and water conservation. 展开更多
关键词 Dongjiang Lake Reservoir area stand Surface runoff Forest management
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