Does Green Food Certification promote agri-food export quality?Evidence from China

The construction of a food certification system plays a vital role in upgrading export quality, which previous studies have largely overlooked. We match China's industry-level data of Green Food Certification with its HS6-digit export data of agri-food products to quantify the impact of Green Food Certification on export quality. We identify the significant and positive effect of Green Food Certification on export quality. The 2SLS estimation based on instrumental variables and a range of robustness checks confirm the validity and robustness of the benchmark conclusions. Further analysis discloses that Green Food Certification improves export quality by raising agricultural production efficiency and brand premiums. Heterogeneity analysis shows that the effect of Green Food Certification varies across regions, notably improving the quality of agri-food products exported to developed regions and regions with high levels of import supervision. Furthermore, among various product types, Green Food Certification significantly improves the export quality of primary products and products vulnerable to non-tariff measures. The above findings could guide the future development of agri-food quality certification systems, potentially leading to a transformation and promotion of the agri-food trade.

How Marie Sklodowska-Curie Doctoral Networks Can Support European Refractory Ecosystem in Context of The European Green Deal

The movement of the Iron&Steelmaking(I&S)industry towards Net-Zero emissions and digitalized processes through disruptive,breakthrough technologies will be achieved through the use of Hydrogen.The biggest challenge for the refractory industry is to continue to meet the performance expectations while,at the same time,moving to a more sustainable production direction.The complexity and urgency of these technological changes,highlighted by the European Green Deal,requires ambitious,international,interdisciplinary and intersectoral projects,bringing together institutes from across the global value chain,to carry out cutting edge research.The European Union,through its flagship doctoral training program,MSCA,has,and continues to support research and development as well as the promotion of the refractory industry in Europe.An introduction to two MSCA projects and some of the results achieved are highlighted within this article.

水禽细小病毒SYBR Green Ⅰ荧光定量PCR检测方法的建立与应用

为了建立水禽细小病毒(WPV)快速检测方法,根据序列比对结果在水禽细小病毒NS基因SF3保守区域内设计特异性引物,建立SYBR Green Ⅰ荧光定量PCR通用检测方法。该方法的扩增效率(E)为90.0%,相关系数(R~2)=0.99,标准曲线方程为y=-3.607x+38.77;除WPV出现S形扩增曲线外,新城疫病毒(NDV)、H9亚型禽流感病毒(H9 AIV)、鸭坦布苏病毒(DTMUV)、鸭肝炎病毒(DHAV)、鸭肠炎病毒(DEV)、鸭呼肠孤病毒(DRV)样品均未出现S形阳性扩增曲线;批内变异系数(CV)为0.15%~0.23%,批间变异系数为0.09%~0.28%。结果表明,SYBR Green Ⅰ荧光定量PCR检测方法重复性好、灵敏度高和特异性强。临床样品检测结果表明,SYBR Green Ⅰ荧光定量PCR与普通PCR的符合率达98.4%,灵敏度是普通PCR的1 000倍。SYBR Green Ⅰ荧光定量PCR检测方法不仅能定性检测WPV,还可以进行定量检测,可用于种鸭场、种鹅场的WPV净化检测,也可用于WPV临床大量样品的快速检测。

盆底超声检查和Green分型诊断产妇产后早期盆底结构及压力性尿失禁

目的:探究盆底超声检查和Green分型在产妇产后早期盆底结构及压力性尿失禁(SUI)诊断中的价值。方法:选取2021年3月-2023年3月本院就诊经产妇204例,根据产后早期(6~8周)SUI发生情况分为SUI组(82例)和非SUI组(122例)。检查产妇静息和Valsalva动作状态下膀胱颈距耻骨联合下缘的距离(BSD)、膀胱尿道后角(PUA)、膀胱颈移动度(BND)和尿道旋转角(UR),对比Green分型;采用受试者工作特征(ROC)曲线分析相关参数诊断SUI价值。结果:静息状态下,两组盆底超声参数无差异(P>0.05);SUI组Valsalva动作状态下的BSD、PUA、BND和UR值均大于非SUI组,Green分型Ι型占比(25.6%)低于非SUI组(59.8%)(均P<0.05)。ROC曲线分析,Green分型曲线下面积(AUC)0.579,对SUI诊断价值不佳(P=0.057),Valsalva动作状态下BSD(AUC=0.758)、PUA(AUC=0.743)、BND(AUC=0.769)和UR(AUC=0.705)对SUI诊断价值良好(均P<0.05)。结论:盆底超声检查和Green分型能有效评估经产妇早期盆底结构状况,前者可诊断SUI。

约束Green函数与非线性地基梁模态分析

在生物和医学领域,微机电系统(MEMS)中的微梁结构在植入人体的使用时,由于体内的细胞环境类似于水凝胶,在这种环境下工作,设备和仪器的精度和稳定性很大程度上受到细胞弹性的影响.为了分析此类地基梁的动力学问题,本文建立了非线性基础上的梁振动模型,研究了任意位置弹簧和非线性弹簧基础上的梁模态.通过Laplace变换和线性叠加原理,得到了一种约束Green函数,利用数值计算验证方案的有效性,并研究了各种重要物理参数的影响,发现弹簧位置向跨中移动时,模态对称性被打破,弹簧刚度增加,模态阶数改变.

猪流行性腹泻病毒SYBR Green I实时荧光定量PCR检测方法的建立

为了建立高效、灵敏的猪流行性腹泻病毒(PEDV)检测方法,本研究从GenBank数据库中获取PEDV N基因序列,扩增出PEDV N基因标准质粒,并在N基因的保守区域内设计了一对特异性荧光定量引物,成功建立了SYBR Green I实时荧光定量PCR检测方法。经过一系列试验表明,该检测方法线性关系良好,R^(2)值为0.99;特异性强,敏感性高,最低可检测至2.23 copies/μL,比普通PCR灵敏约100倍;重复性好,组内变异系数为0.25%~0.43%,组间变异系数为0.67%~0.97%;对于各地区96份临床样品检测出PEDV阳性率为25%。本研究建立的实时荧光定量PCR检测方法为PEDV的临床诊断、流行病学调查以及定量研究提供了有效的检测工具。

How do green product exports affect carbon emissions?Evidence from China

Green development has become an increasingly important global initiative.Therefore,this study focuses on the impact of green product exports on carbon emissions in China.Firm-level datasets are used to estimate provincial green product exports between 2001-2016.This study estimates the impact of green exports on carbon emissions using the system generalized method of moments methodology.In addition,heterogeneity and asymmetry of the nexus are explored.The following findings are highlighted:(1)China’s green product exports show a significant upward trend during the study period;(2)increased green product exports,especially those of high-technology manufacturers,can effectively mitigate CO_(2) emissions;(3)the influence of green product exports on carbon emissions is regional heterogeneous but consistent across quantiles;and(4)technique and scale effects are significant mediators,whereas the composition effect is not.These findings provide new evidence supporting methods to mitigate China’s CO_(2) emissions by adjusting green product export structures.

Laparoscopic choledocholithotomy and transductal T-tube insertion with indocyanine green fluorescence imaging and laparoscopic ultrasound: A case report

BACKGROUND Laparoscopic choledocholithotomy for a large impacted common bile duct(CBD)stone is a challenging procedure because of the technical difficulty and the possibility of postoperative complications,even in this era of minimally invasive surgery.Herein,we present a case of large impacted CBD stones.CASE SUMMARY A 71-year-old man showed a distal CBD stone(45 mm×20 mm)and a middle CBD stone(20 mm×15 mm)on computed tomography.Endoscopic retrograde cholangiopancreatography failed due to the large size of the impacted stone and the presence of a large duodenal diverticulum.Laparoscopic choledocholithotomy was decided,and we used a near-infrared indocyanine green fluorescence scope to detect and expose the supraduodenal CBD more accurately.Then,the location,size,and shape of the stones were detected using a laparoscopic intraoperative ultrasound.The CBD was opened with a 2-cm-sized vertical incision.After irrigating several times,two CBD stones were removed with the Endo BabcockTM.T-tube insertion was done for postoperative cholangiography and delayed the removal of remnant sludge.The patient had no postoperative complications.CONCLUSION Laparoscopic choledocholithotomy by transcholedochal approach and transductal T-tube insertion is a safe and feasible option for large-sized impacted CBD stones.

美洲鳗鲡腺瘤病毒(AEAdoV)普通PCR和SYBR Green I实时荧光定量PCR检测方法的建立及应用

为建立美洲鳗鲡腺瘤病毒(AEAdoV)的检测方法,根据AEAdoV福建株(AEAdoVFJ)的superfamily 3 helicases(S3H)序列,设计引物,建立了AEAdoV的普通PCR和qPCR检测方法;进一步评价检测方法的灵敏性、特异性及重复性,利用2种方法对美洲鳗鲡“出血性烂鳃”病料进行了检测,并对美洲鳗鲡体内不同组织的病毒含量进行分析。结果显示,普通PCR扩增的目的片段长度约300 bp,利用其构建的qPCR质粒标准品,其拷贝数与qPCR阈值循环数(C_(t))线性关系良好,线性范围广,标准曲线相关系数(R2)达到0.999,扩增效率为105.067%。建立的普通PCR法和qPCR的最低检测AEAdoV拷贝数分别为100个和10个。2种方法均可特异性检测AEAdoV,而对蛙虹彩病毒(RGV)、鳗鲡疱疹病毒(AngHV)、鲤疱疹病毒(KHV)、对虾白斑综合征病毒(WSSV)、日本鳗鲡内皮细胞坏死病毒(JEAdoV)和花鳗鲡腺瘤病毒(MEAdoV)均无扩增反应。qPCR法的组内和组间变异系数均小于2%,表明其重复性良好。临床应用结果显示,35份美洲鳗鲡“出血性烂鳃”病料,采用普通PCR法的AEAdoV检出率为82.8%,而qPCR法的AEAdoV检出率为97%。对美洲鳗鲡不同组织的病毒含量分析结果显示,心脏、肝脏、鳃、鳍的AEAdoV相对含量较高,而黏液、皮肤和脾脏的病毒含量相对较低。研究表明,建立的AEAdoV的灵敏度高、特异性强的普通PCR和qPCR检测方法,证实AEAdoV与美洲鳗鲡“出血性烂鳃”病密切相关,且在感染鳗鲡主要组织中都存在。实验结果对于研究AEAdoV的致病性,开展其流行情况和病原学情况分析具有重要意义。

香辛料SYBR GREENⅠ染料实时聚合酶链式反应检测方法的建立及应用

目的:本研究致力于建立香辛料真实性成分的分子鉴定方法,为香辛料的掺假提供有效的鉴定和检测方法,在不同的使用条件下分别实现定性或半定量分析,为建立相关标准提供一定的基础技术支撑。方法:研究采用SYBR GREENⅠ染料实时聚合酶链式反应(polymerase chain reaction,PCR)方法,通过设计特异性引物成功建立了特异性检测八角、茴香、胡椒和花椒的分析技术。结果:该方法能够特异性检测出八角、茴香、胡椒、花椒,Ct值在标准品体积分数0.001%~10%范围内线性关系良好。针对八角标准品,检测限为1%;针对茴香、花椒、胡椒标准品,最检测限均为0.001%。利用该方法对5份实际样本进行检测,分别在样本1中未检测出八角、茴香、胡椒、花椒;样本2、3、5中检测出八角、茴香、胡椒;样本4中检测出八角、茴香、胡椒、花椒。结论:本实验建立的SYBR GREENⅠ染料实时PCR方法灵敏度高、重复性好,可应用于实际样品的检测。

SYBR GreenⅠ染料-实时荧光定量聚合酶链式反应法检测发酵乳中的霉菌和酵母含量

目的建立一种应用SYBR GreenⅠ染料的实时荧光定量聚合酶链式反应法快速检测发酵乳中的霉菌和酵母菌。方法本研究针对霉菌与酵母菌的保守序列设计引物,确定最优反应体系与反应条件。通过熔解曲线以及非目标菌株的检测验证该方法的特异性;通过菌悬液的梯度稀释检测确定该方法的灵敏度;通过菌悬液与发酵乳样品混合后进行检测,确定该方法的检出限,并得到标准曲线。结果该方法能够特异性的检测发酵乳中的霉菌、酵母菌,无交叉反应。该方法检测霉菌、酵母菌的灵敏度均为10^(2) CFU/mL,并且当菌悬液与发酵乳样品混合后,并没有降低该方法的检出限。在10^(2)~10^(6) CFU/mL浓度范围内,菌液浓度的对数值与循环阈值(cyclethreshold,Ct)具有良好的线性关系,可以在市售样品的检测中通过Ct值计算样品中目标菌的含量,更快的判断发酵乳是否被霉菌、酵母菌污染。结论该方法可用于发酵乳中霉菌和酵母菌的快速检测,为发酵乳的食品安全提供了技术保障。

循证护理在降低肺癌患者PORT并发症中的应用效果

目的:评估循证护理在降低肺癌患者植入式静脉输液港(PORT)相关并发症中的应用效果。方法:选取2021年1月—2023年6月江苏省肿瘤医院确诊肺癌并行PORT植入化疗的150例患者,根据护理方式的不同分为对照组和观察组,每组75例。对照组给予常规护理,观察组给予循证护理。比较两组的并发症发生率、情绪状态、知识掌握度评分。结果:观察组PORT相关性并发症总发生率低于对照组,差异有统计学意义(P<0.05)。护理前,两组不良情绪评分比较,差异无统计学意义(P>0.05);护理后,观察组焦虑自评量表(SAS)、抑郁自评量表(SDS)评分低于对照组,差异有统计学意义(P<0.05)。观察组知识掌握度评分均高于对照组,差异有统计学意义(P<0.05)。结论:循证护理在降低肺癌患者PORT并发症发生率、改善患者心理状态和生活质量方面效果显著,值得临床中应用。

Research on the Application of Green Lighting Technology in Sports Lighting

With China’s continued development of society and economy, the importance of ecological environmental protection is growing. This protection has become an indispensable part of social development. The use of various green environmental protection equipment can effectively promote ecological environmental protection and reduce the adverse effects of human activities on the environment. In the field of sports lighting equipment in China, traditional lighting methods constitute an inherent danger to the environment, leading to a waste of valuable resources and environmental pollution. This study involves a multi-tile-multifunctional-function controller, which effectively solves the control problem of the control light. In the sports lighting group, the high and low-level lighting of the sports lighting group, and adjust the light from the height highly effectively to adjust each height. The low-shot mode reduces the power consumption of the system by about 33% to 60%, which significantly helps energy saving. By optimizing the performance of the lighting system, promoting the preservation of power resources, reducing the adverse effects of pollution caused by equipment utilization and energy waste, and promoting the harmonious cohabitation of human and natural environment. .

Importance of Urban Wetland Parks under the Development of Green Healthy Cities:A Case Study of Nanjing City

Wetlands are widely distributed all over the world,and have many wildlife resources,which are the main pieces of the puzzle for natural resource conservation and sustainable development on earth and have important irreplaceability.In this paper,through questionnaire survey,field research,literature review,etc.,importance weight analysis was conducted by using principal component analysis,and field survey and questionnaire were carried out to collect data on ecological environment function,environmental protection function,landscape beautification function,disaster prevention and mitigation function of urban wetlands.The problems in wetland parks of Nanjing were discussed,such as lack of awareness of landscape planning,deficient late management of wetland parks,weak ability of sustainable development,and unreasonable landscape layout and function.Finally,corresponding solutions were proposed,such as adhering to the planning and design of urban wetland parks with green as the base and health as the basis,persisting in the construction of a wetland system with high biodiversity and near-natural characteristics,adhering to the principle of sustainable development,adopting the construction idea of symbiosis and circulation of urban wetland parks,strengthening education and publicity work,and paying attention to the organic combination of system protection and coordinated construction.The research can build a new development direction for the model of urban wetland parks and green healthy cities,and provide theoretical support for urban sustainable construction.

和美乡村视角下的苏南农田“GREEN”水环境治理模式应用研究

随着中国宜居宜业和美乡村建设的不断深入,乡村水环境的治理与保护作为乡村生态文明建设的重要组成部分日益受到社会的重视和关注。在以往大规模灰色基础设施建设的背景下,乡村忽略了对于水生态绿色基础设施的构建,生态格局趋于破碎。农田空间作为农业面源污染的重要源头,结合多学科视角探索农田水环境污染治理新模式具有高度的必要性。立足于苏南地区独特的农田水网体系,紧密结合农业空间生产属性,研究现有农田水环境治理理论方法与实际建设,提炼以“源头绿色—过程净化—末端吸收—生态保育—循环回用”的苏南农田“GREEN”水环境治理模式,探索风景园林学科水治理方法在农田沟渠、河道及湿地等乡村空间的运用路径,并以常州市中天黄金大农场总体规划为例进行实践应用。推动风景园林学科于乡村生产空间的应用模式探索,为未来农业面源污染防治提供参考借鉴。

基于port write事件触发的RapidIO节点动态管理方法

针对现有大规模的综合化电子信息系统中RapidIO节点管理实时性不强、CPU资源占用多的问题,提出了一种基于port write事件触发的RapidIO节点动态管理方法。该方法利用RapidIO交换芯片的port write包上报退网或者入网的节点信息,通过该信息动态管理RapidIO网络中的所有节点。工程应用实测结果表明,该方法实时性高,CPU资源占用少。

Basilic vein variation encountered during surgery for arm vein port:A case report

BACKGROUND Venous variations are uncommon and usually hard to identify,and basilic vein variation is particularly rare.Basilic vein variation usually presents without any clinical symptoms and is often regarded as a benign alteration.This case was a patient with congenital basilic vein variation encountered during surgery for an infusion port.CASE SUMMARY We documented and analyzed an uncommon anatomical variation in the basilic vein encountered during arm port insertion.This peculiarity has hitherto remained undescribed in the literature.We offer remedial strategies for addressing this anomaly in the future and precautionary measures to circumvent its occurrence.We conducted a comprehensive review of analogous cases in the literature,offering pertinent therapeutic recommendations and solutions,with the aim of enhancing the efficacy and safety of future arm port implantations.CONCLUSION Venous variation is rare and requires detailed intraoperative and postoperative examination to ensure accuracy,so as not to affect subsequent treatment.

Effect of a Commercial Extract of Green Tea and a Pure Catechin on Two Veillonella strains

The catechin Epigallocatechin-3-O-Gallate (EGCG) which is found in of Green Tea extracts (GTE), displays a variety of microbicidal properties. It is largely believed that EGCG inhibits the growth of cariogenic and periodontopathic bacteria. Objective: In this paper we compared the inhibitory activity of EGCG and a commercial GTE on the growth of Veillonella parvula. Chlorhexidine was used as positive control. Methodology: V. parvula ATCC 10790 and a clinical isolate obtained from a periodontal disease patient were cultured in the presence of EGCG or a commercial GTE, and the measurements of bacterial growth inhibition were compared to the values obtained with 0.12 and 0.2% chlorhexidine. Results: Chlorhexidine inhibited bacterial growth, however in contrast to a previous report, neither EGCG nor the GTE showed any effect on bacterial growth. Conclusions: The data show and confirm that chlorhexidine is a growth inhibitor of V. parvula while EGCG and GTE do not display such effect.

The Impact of Privatization on Terminal Efficiency: A Case Study of Tema Port

Port and terminal efficiency are of utmost importance to the container shipping industry due to their significance in enhancing the competitive advantage of ports within a region. Consequently, there have always been notable variations of studies around it. This paper analyzes the impact of privatization on terminal efficiency using the Port of Tema as a Case Study. The main objective of this paper is to analyze the efficiency trends of the public and private terminals in the port over the years. To achieve this objective, DEA-CCR methodology was employed to calculate the annual technical efficiency trends of the private and public terminals using four input variables and three output variables. The main results of the paper indicated that the public and private terminals were efficient for multiple years. However, the efficiency scores over the years demonstrated inconsistency, exhibiting notable fluctuations. The findings of this study will aid policymakers across the region on policies relating to the efficiency and ownership structure of ports and terminals.

Green Synthesis of Iron(Ⅱ,Ⅲ)-polyphenol Nanoparticles and Their Adsorption of Malachite Green

Three kinds of iron nanoparticles(FeNPs)were prepared via green route based on pomegranate(PG),green tea(GT),and mulberry(ML)extracts under ambient conditions.The obtained materials were characterized by scanning electron microscopy(SEM),transmission electronic microscopy(TEM),X-ray energy-dispersive spectrometer(EDS),X-ray diffraction(XRD),fourier transform infrared spectroscopy(FTIR),and X-ray photoelectron spectroscopy(XPS)techniques.The experimental results show that FeNPs were in the form of amorphous iron(Ⅱ,Ⅲ)-polyphenol complex with different dispersity and morphologies.GT-Fe has the smallest size range of 25-35 nm,PG-Fe has a moderate size-distribution of 30-40 nm,while ML-Fe formed a tuberous net-type with a sheeting structure.PG-Fe displays the highest removal efficiency of 90.2%in 20 min towards cationic dye of malachite green(16.6%by ML-Fe and 69.3%by GT-Fe),which is attributed to its highest polyphenol content,lowest zeta potential,as well as the most Fe^(2+)on the surface of FeNPs.The removal mechanism was mainly induced by electrostatic adsorption based on pH and zeta potential tests.