A total of 126 traditional villages in Guangdong were selected for research to analyze their spatial distribution pattern, spatial autocorrelation, and Han subgroup characteristics through the spatial analysis with GI...A total of 126 traditional villages in Guangdong were selected for research to analyze their spatial distribution pattern, spatial autocorrelation, and Han subgroup characteristics through the spatial analysis with GIS. The results showed that the traditional villages in Guangdong were distributed compactly, which was similar to that in other provinces; on the prefecture-level city scale, they were mainly distributed in Meizhou, Qingyuan, Zhanjiang, and Zhaoqing, showing a central distribution; on the regional scale, they were mainly distributed in northern Guangdong and the Pearl River Delta, indicating an unbalanced distribution; their density of spatial distribution showed a significant difference with a provincial distribution density of 0.59 per thousand km^2 and the highest distribution density of 2.14 per thousand km^2; there were four gathering areas accommodating many traditional villages, namely Meizhou-Chaoshan area, Guangfo-Dongguan area, Leizhou in Zhanjiang, and Lianzhou in Qingyuan; the spatial distribution of traditional villages, which presented a pattern of hot spot—sub-hot spot—sub-cold spot—cold spot, had a significant global spatial autocorrelation, and areas in similar sizes were tending towards gathering; traditional villages in Guangdong belonged to the Cantonese-speaking group and Hakka-speaking group with a density of 0.59 per thousand km^2 and 0.48 per thousand km^2 respectively, and Han subgroup with the highest distribution density was the minority areas of 1.31 per thousand km^2, followed by Shaozhou dialect area with a density of 1.00 per thousand km^2.展开更多
Background The association of E670G polymorphism in the proprotein convertase subtilisin/kexin type 9 (PCSK9) gene and serum lipid profiles is inconsistent in dif- ferent ethnic groups.Bai Ku Yao is a special subgroup...Background The association of E670G polymorphism in the proprotein convertase subtilisin/kexin type 9 (PCSK9) gene and serum lipid profiles is inconsistent in dif- ferent ethnic groups.Bai Ku Yao is a special subgroup of the Yao minority in China.The present study was undertaken association of PCSK9 E670G polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.Methods A total of 649 subjects of Bai Ku Yao and 646 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples.Genotyping of the PCSK9 E670G polymorphism was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing. Results The levels of serum total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C) and apolipoprotein(Apo) AI were lower in Bai Ku Yao than in Han(P【0.01 for all).The frequency of A and G alleles was 98.00%and 2.00%in Bai Ku Yao,and 95.20%and 4.80%in Han(P【0.01);respectively. The frequency of AA,AG and GG genotypes was 95.99%,4.01%and 0%in Bai Ku Yao,and 91.02%, 8.36%and 0.62%in Han(P【0.01);respectively.There were also significant differences in the genotypic and allelic frequencies between n and the ratio of ApoAI to ApoB in Han Chinese but not in Bai Ku Yao were different between the AA and AG/GG genotypes(P【0.05 for all).The G allele carriers had higher serum HDL-C and higher ApoAI to ApoB ratio than the G allele noncarriers.When serum lipid parameters in Han were analyzed according to sex,the G allele carriers had higher serum HDL and ApoAI levels in males (P【0.05),and lower ApoB level and higher ApoAI to ApoB ratio in females(P【0.05 for all).Multiple linear regression analysis showed that serum HDL-C levels were correlated with genotypes in both ethnic groups(P【0.05 each).Serum lipid parameters were also correlated with sex,age,body massindex,alcohol consumption,cigarette smoking,and blood pressure in both ethnic groups(P【0.05-0.001).Conclusions These results suggest that the PCSK9 E670G polymorphism is mainly associated with some serum lipid parameters in the Han population,both gender show different relations to different serum lipid parameters.The G allele carriers might have higher serum lipid profiles than the G allele noncarriers. ormal LDL-C(≤3.20 mmol/L) and high LDL-C subgroups (】 3.20 mmol/L,P【0.01;respectively) in Bai Ku Yao, and between normal ApoB(≤1.14 g/L) and high ApoB subgroups(】 1.14 g/L,P 【 0.01;respectively) in Han.展开更多
Objectives The association of methylenetetrahy-drofolate reductase(MTHFR) gene polymorphism and serum lipid profiles is still controversial in diverse ethnics.Bai Ku Yao is an isolated subgroup of the Yao minority in ...Objectives The association of methylenetetrahy-drofolate reductase(MTHFR) gene polymorphism and serum lipid profiles is still controversial in diverse ethnics.Bai Ku Yao is an isolated subgroup of the Yao minority in China. The aim of the present study was to eveluate the association of MTHFR C677Tpolymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.Methods A total of 780 subjects of Bai Ku Yao and 686 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples.Genotyping of the MTHFR C677T was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing.Results The levels of serum total cholesterol(TC),high-density lipoprotein cholesterol (HDL-C),low-density lipoprotein cholesterol(LDL-C), apolipoprotein(Apo) AI and ApoB were lower in Bai Ku Yao than in Han(P【0.05-0.001).The frequency of C and T alleles was 77.4%and 22.6%in Bai Ku Yao,and 60.9%and 39.1%in Han(P【0.001);respectively.The frequency of CC,CT and TT genotypes was 58.7%,37.3%and 4.0%in Bai Ku Yao,and 32.6%,56.4%and 11.0%in Han(P【 0.001);respectively.The levels of TC and LDL-C in both ethnic groups were significant differences among the three genotypes(P【0.05-0.01).The T allele carriers had higher serum TC and LDL-C levels than the T allele noncarriers. The levels of ApoB in Han were significant differences among the three genotypes(P【0.05).The T allele carriers had higher serum ApoB levels as compared with the T allele noncarriers. The levels of TC,TG and LDL-C in Bai Ku Yao were correlated with genotypes(P【0.05-0.001),whereas the levels of LDL-C in Han were associated with genotypes(P【 0.001).Serum lipid parameters were also correlated with sex, age,body massindex,alcohol consumption,cigarette smoking, and blood pressure in the both ethnic groups.Conclusions The differences in serum TC,TG,LDL-C and ApoB levels between the two ethnic groups might partly result from different genotypic and allelic frequencies of the MTHFR C677Tor differentMTHFR gene-enviromental interactions.展开更多
Objectives The association of peroxisome prolif-erator -activated receptor delta(PPARD) +294T】C polymorphism and serum lipid levels is inconsistent in several previous studies.Bai Ku Yao is an isolated association of...Objectives The association of peroxisome prolif-erator -activated receptor delta(PPARD) +294T】C polymorphism and serum lipid levels is inconsistent in several previous studies.Bai Ku Yao is an isolated association of PPARD +294T】C(rs2016520) polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.Methods A total of 609 subjects of Bai Ku Yao and 573 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples.Genotyping of the PPARD +294T】C polymorphism was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis, and then confirmed by direct sequencing.Results The levels of serum total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),apolipoprotein(Apo) AI and ApoB were lower in Bai Ku Yao than in Han(P【0.001 for all).The frequency of T and C alleles was 77.50%and 22.50%in Bai Ku Yao,and 72.43%and 27.57%in Han (P【0.01);respectively.The frequency of TT,TC and CC genotypes was 60.59%,33.83%and 5.53%in Bai Ku Yao, and 52.18%,40.50%and 7.32%in Han(P【0.05);respectively. The levels of LDL-C,ApoB and the ratio of ApoAI to ApoB in Bai Ku Yao were different among the three genotypes in females but not in males(P【0.05 for all).The subjects with TT and TC genotypes had lower serum LDL-C and ApoB levels and higher the ratio of ApoAI to ApoB than the CC genotype in females.The levels of TC and ApoB in the total Han population were different among the three genotypes (P【0.05 for all).The C allele carriers had higher serum TC and ApoB levels than the C allele noncarriers.When serum lipid levels were analyzed according to sex,the difference in serum TC levels in Han was significant in males(P【0.01) but not in females,whereas the difference in serum ApoB levels was significant in females(P【0.05)but not in males. Serum TC and ApoB levels were correlated with genotypes in Han(P【0.05 for each) but not in Bai Ku Yao.Serum lipid parameters were also correlated with sex,age,body massindex, alcohol consumption,cigarette smoking,and blood pressure in both ethnic groups.Conclusions These results suggest that the association of PPARD +294T】C polymorphism and serum lipid levels is different between the Bai Ku Yao and Han populations.The discrepancy between the two ethnic groups might partly result from different PPARD +294T】C polymorphism or PPARD gene-enviromental interactions,subgroup of the Yao minority in China.展开更多
Objectives Endotheliallipase(EL) is a major determinantof high-density lipoprotein-cholesterol(HDL-C) metabolism,but the association of endothelial lipase gene (LIPG) polymorphism and serum HDL-C levels is scarce and ...Objectives Endotheliallipase(EL) is a major determinantof high-density lipoprotein-cholesterol(HDL-C) metabolism,but the association of endothelial lipase gene (LIPG) polymorphism and serum HDL-C levels is scarce and conflicting in diverse populations.Bai Ku Yao is an isolated subgroup of the Yao minority in China.This study was designed to detect the association of LIPG 584C】T(rs2000813) polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations. Methods A total of 645 subjects of Bai Ku Yao and 638 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples.Genotyping of the LIPG584C】T was performed by polymerse chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing. Results The levels of serum total cholesterol(TC),HDL-C, low-density lipoprotein cholesterol(LDL-C) and apolipoprotein (Apo) AI and ApoB were lower in Bai Ku Yao than in Han(P【0.05-0.001).The frequency of C and T alleles was 73.5%and 26.5%in Bai Ku Yao,and 67.9%and 32.1%in Han(P【0.01).The frequency of CC,CT and TT genotypes was 50.4%,46.2%and 3.4%in Bai Ku Yao,and 41.4%, 53.1%and 5.5%in Han(P【0.01);respectively.Serum HDL-C levels in both ethnic groups were different among the three genotypes(P【0.05 for each).Serum TC levels in both ethnic groups were also different between CC and CT/TT types(P【 0.05 for each).The T allele carriers had higher serum HDL-C and TC levels than the T allele noncarriers.Multivariate logistic regression analysis showed that the levels of HDL-C and ApoB were correlated with genotype in Bai Ku Yao(P【 0.05 for each),whereas the levels of TC and HDL-C were associated with genotype in Han Chinese(P【0.05 and P【 0.01).Serum lipid parameters were also correlated with gender, age,weight,body massindex,alcohol consumption, cigarette smoking,and blood pressure in the both ethnic groups.Conclusions The frequency of LIPG 584Tallele is lower in the Bai Ku Yao than in the Han populations.The LIPG 584Tallele is associated with serum HDL-C and TC levels.The differences in serum lipid profiles between the two ethnic groups might partly result from different genotypic and allelic of LIPG 584C 】T or different LIPG-enviromental interactions.展开更多
基金Sponsored by Guangdong Provincial Natural Science Foundation(2015A030313852)Guangdong Provincial Scientific and Technological Programs(2014A020219006,2015B070701020)
文摘A total of 126 traditional villages in Guangdong were selected for research to analyze their spatial distribution pattern, spatial autocorrelation, and Han subgroup characteristics through the spatial analysis with GIS. The results showed that the traditional villages in Guangdong were distributed compactly, which was similar to that in other provinces; on the prefecture-level city scale, they were mainly distributed in Meizhou, Qingyuan, Zhanjiang, and Zhaoqing, showing a central distribution; on the regional scale, they were mainly distributed in northern Guangdong and the Pearl River Delta, indicating an unbalanced distribution; their density of spatial distribution showed a significant difference with a provincial distribution density of 0.59 per thousand km^2 and the highest distribution density of 2.14 per thousand km^2; there were four gathering areas accommodating many traditional villages, namely Meizhou-Chaoshan area, Guangfo-Dongguan area, Leizhou in Zhanjiang, and Lianzhou in Qingyuan; the spatial distribution of traditional villages, which presented a pattern of hot spot—sub-hot spot—sub-cold spot—cold spot, had a significant global spatial autocorrelation, and areas in similar sizes were tending towards gathering; traditional villages in Guangdong belonged to the Cantonese-speaking group and Hakka-speaking group with a density of 0.59 per thousand km^2 and 0.48 per thousand km^2 respectively, and Han subgroup with the highest distribution density was the minority areas of 1.31 per thousand km^2, followed by Shaozhou dialect area with a density of 1.00 per thousand km^2.
文摘Background The association of E670G polymorphism in the proprotein convertase subtilisin/kexin type 9 (PCSK9) gene and serum lipid profiles is inconsistent in dif- ferent ethnic groups.Bai Ku Yao is a special subgroup of the Yao minority in China.The present study was undertaken association of PCSK9 E670G polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.Methods A total of 649 subjects of Bai Ku Yao and 646 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples.Genotyping of the PCSK9 E670G polymorphism was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing. Results The levels of serum total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C) and apolipoprotein(Apo) AI were lower in Bai Ku Yao than in Han(P【0.01 for all).The frequency of A and G alleles was 98.00%and 2.00%in Bai Ku Yao,and 95.20%and 4.80%in Han(P【0.01);respectively. The frequency of AA,AG and GG genotypes was 95.99%,4.01%and 0%in Bai Ku Yao,and 91.02%, 8.36%and 0.62%in Han(P【0.01);respectively.There were also significant differences in the genotypic and allelic frequencies between n and the ratio of ApoAI to ApoB in Han Chinese but not in Bai Ku Yao were different between the AA and AG/GG genotypes(P【0.05 for all).The G allele carriers had higher serum HDL-C and higher ApoAI to ApoB ratio than the G allele noncarriers.When serum lipid parameters in Han were analyzed according to sex,the G allele carriers had higher serum HDL and ApoAI levels in males (P【0.05),and lower ApoB level and higher ApoAI to ApoB ratio in females(P【0.05 for all).Multiple linear regression analysis showed that serum HDL-C levels were correlated with genotypes in both ethnic groups(P【0.05 each).Serum lipid parameters were also correlated with sex,age,body massindex,alcohol consumption,cigarette smoking,and blood pressure in both ethnic groups(P【0.05-0.001).Conclusions These results suggest that the PCSK9 E670G polymorphism is mainly associated with some serum lipid parameters in the Han population,both gender show different relations to different serum lipid parameters.The G allele carriers might have higher serum lipid profiles than the G allele noncarriers. ormal LDL-C(≤3.20 mmol/L) and high LDL-C subgroups (】 3.20 mmol/L,P【0.01;respectively) in Bai Ku Yao, and between normal ApoB(≤1.14 g/L) and high ApoB subgroups(】 1.14 g/L,P 【 0.01;respectively) in Han.
文摘Objectives The association of methylenetetrahy-drofolate reductase(MTHFR) gene polymorphism and serum lipid profiles is still controversial in diverse ethnics.Bai Ku Yao is an isolated subgroup of the Yao minority in China. The aim of the present study was to eveluate the association of MTHFR C677Tpolymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.Methods A total of 780 subjects of Bai Ku Yao and 686 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples.Genotyping of the MTHFR C677T was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing.Results The levels of serum total cholesterol(TC),high-density lipoprotein cholesterol (HDL-C),low-density lipoprotein cholesterol(LDL-C), apolipoprotein(Apo) AI and ApoB were lower in Bai Ku Yao than in Han(P【0.05-0.001).The frequency of C and T alleles was 77.4%and 22.6%in Bai Ku Yao,and 60.9%and 39.1%in Han(P【0.001);respectively.The frequency of CC,CT and TT genotypes was 58.7%,37.3%and 4.0%in Bai Ku Yao,and 32.6%,56.4%and 11.0%in Han(P【 0.001);respectively.The levels of TC and LDL-C in both ethnic groups were significant differences among the three genotypes(P【0.05-0.01).The T allele carriers had higher serum TC and LDL-C levels than the T allele noncarriers. The levels of ApoB in Han were significant differences among the three genotypes(P【0.05).The T allele carriers had higher serum ApoB levels as compared with the T allele noncarriers. The levels of TC,TG and LDL-C in Bai Ku Yao were correlated with genotypes(P【0.05-0.001),whereas the levels of LDL-C in Han were associated with genotypes(P【 0.001).Serum lipid parameters were also correlated with sex, age,body massindex,alcohol consumption,cigarette smoking, and blood pressure in the both ethnic groups.Conclusions The differences in serum TC,TG,LDL-C and ApoB levels between the two ethnic groups might partly result from different genotypic and allelic frequencies of the MTHFR C677Tor differentMTHFR gene-enviromental interactions.
文摘Objectives The association of peroxisome prolif-erator -activated receptor delta(PPARD) +294T】C polymorphism and serum lipid levels is inconsistent in several previous studies.Bai Ku Yao is an isolated association of PPARD +294T】C(rs2016520) polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.Methods A total of 609 subjects of Bai Ku Yao and 573 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples.Genotyping of the PPARD +294T】C polymorphism was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis, and then confirmed by direct sequencing.Results The levels of serum total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),apolipoprotein(Apo) AI and ApoB were lower in Bai Ku Yao than in Han(P【0.001 for all).The frequency of T and C alleles was 77.50%and 22.50%in Bai Ku Yao,and 72.43%and 27.57%in Han (P【0.01);respectively.The frequency of TT,TC and CC genotypes was 60.59%,33.83%and 5.53%in Bai Ku Yao, and 52.18%,40.50%and 7.32%in Han(P【0.05);respectively. The levels of LDL-C,ApoB and the ratio of ApoAI to ApoB in Bai Ku Yao were different among the three genotypes in females but not in males(P【0.05 for all).The subjects with TT and TC genotypes had lower serum LDL-C and ApoB levels and higher the ratio of ApoAI to ApoB than the CC genotype in females.The levels of TC and ApoB in the total Han population were different among the three genotypes (P【0.05 for all).The C allele carriers had higher serum TC and ApoB levels than the C allele noncarriers.When serum lipid levels were analyzed according to sex,the difference in serum TC levels in Han was significant in males(P【0.01) but not in females,whereas the difference in serum ApoB levels was significant in females(P【0.05)but not in males. Serum TC and ApoB levels were correlated with genotypes in Han(P【0.05 for each) but not in Bai Ku Yao.Serum lipid parameters were also correlated with sex,age,body massindex, alcohol consumption,cigarette smoking,and blood pressure in both ethnic groups.Conclusions These results suggest that the association of PPARD +294T】C polymorphism and serum lipid levels is different between the Bai Ku Yao and Han populations.The discrepancy between the two ethnic groups might partly result from different PPARD +294T】C polymorphism or PPARD gene-enviromental interactions,subgroup of the Yao minority in China.
文摘Objectives Endotheliallipase(EL) is a major determinantof high-density lipoprotein-cholesterol(HDL-C) metabolism,but the association of endothelial lipase gene (LIPG) polymorphism and serum HDL-C levels is scarce and conflicting in diverse populations.Bai Ku Yao is an isolated subgroup of the Yao minority in China.This study was designed to detect the association of LIPG 584C】T(rs2000813) polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations. Methods A total of 645 subjects of Bai Ku Yao and 638 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples.Genotyping of the LIPG584C】T was performed by polymerse chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing. Results The levels of serum total cholesterol(TC),HDL-C, low-density lipoprotein cholesterol(LDL-C) and apolipoprotein (Apo) AI and ApoB were lower in Bai Ku Yao than in Han(P【0.05-0.001).The frequency of C and T alleles was 73.5%and 26.5%in Bai Ku Yao,and 67.9%and 32.1%in Han(P【0.01).The frequency of CC,CT and TT genotypes was 50.4%,46.2%and 3.4%in Bai Ku Yao,and 41.4%, 53.1%and 5.5%in Han(P【0.01);respectively.Serum HDL-C levels in both ethnic groups were different among the three genotypes(P【0.05 for each).Serum TC levels in both ethnic groups were also different between CC and CT/TT types(P【 0.05 for each).The T allele carriers had higher serum HDL-C and TC levels than the T allele noncarriers.Multivariate logistic regression analysis showed that the levels of HDL-C and ApoB were correlated with genotype in Bai Ku Yao(P【 0.05 for each),whereas the levels of TC and HDL-C were associated with genotype in Han Chinese(P【0.05 and P【 0.01).Serum lipid parameters were also correlated with gender, age,weight,body massindex,alcohol consumption, cigarette smoking,and blood pressure in the both ethnic groups.Conclusions The frequency of LIPG 584Tallele is lower in the Bai Ku Yao than in the Han populations.The LIPG 584Tallele is associated with serum HDL-C and TC levels.The differences in serum lipid profiles between the two ethnic groups might partly result from different genotypic and allelic of LIPG 584C 】T or different LIPG-enviromental interactions.