Therapeutic effects of Lingguizhugan decoction in a rat model of high-fat diet-induced insulin resistance

BACKGROUND Lingguizhugan(LGZG)decoction is a widely used classic Chinese medicine formula that was recently shown to improve high-fat diet(HFD)-induced insulin resistance(IR)in animal studies.AIM To assess the therapeutic effect of LGZG decoction on HFD-induced IR and explore the potential underlying mechanism.METHODS To establish an IR rat model,a 12-wk HFD was administered,followed by a 4-wk treatment with LGZG.The determination of IR status was achieved through the use of biochemical tests and oral glucose tolerance tests.Using a targeted metabolomics platform to analyze changes in serum metabolites,quantitative real-time PCR(qRT-PCR)was used to assess the gene expression of the ribosomal protein S6 kinase beta 1(S6K1).RESULTS In IR rats,LGZG decreased body weight and indices of hepatic steatosis.It effectively controlled blood glucose and food intake while protecting islet cells.Metabolite analysis revealed significant differences between the HFD and HFDLGZG groups.LGZG intervention reduced branched-chain amino acid levels.Levels of IR-related metabolites such as tryptophan,alanine,taurine,and asparagine decreased significantly.IR may be linked to amino acids due to the contemporaneous increase in S6K1 expression,as shown by qRT-PCR.CONCLUSIONS Our study strongly suggests that LGZG decoction reduces HFD-induced IR.LGZG may activate S6K1 via metabolic pathways.These findings lay the groundwork for the potential of LGZG as an IR treatment.

Protective effects of Lingguizhugan decoction on amyloid-beta peptide (25-35)-induced cell injury Anti-inflammatory effects

In the present study, a human neuroblastoma cell line （SH-SY5Y） and BV-2 microglia were treated with amyloid-β peptide （25-35）, as a model of Alzheimer＇s disease, to evaluate the protective effects of 10-3-10-8 g/mL Lingguizhugan decoction and to examine the underlying anti-inflammatory mechanism. Lingguizhugan decoction significantly enhanced the viability of SH-SY5Y cells with amyloid-β peptide-induced injury, and lowered levels of interleukin-1β, interleukin-6, tumor necrosis factor-a and nitric oxide in the culture supernatant of activated BV-2 microglia. The effects of 103 g/mL Lingguizhugan decoction were more significant. These results suggest that Lingguizhugan decoction can protect SH-SY5Y cells against amyloid-β peptide （25-35）-induced injury in a dose-dependent manner by inhibiting overexpression of inflammatory factors by activated microglia.

The therapeutic effect of Jiawei Danshen Decoction on myocardial ischemia-reperfusion injury by inhibiting H_(2)S-mediated autophagy signaling pathway

Objective To investigate the protective effects of Jiawei Danshen Decoction(加味丹参饮,JWDSD)on myocardial ischemia-reperfusion injury(MIRI)via the regulation of serum Hydrogen sulfide(H2S)and cardiac Beclin1,light Chain 3 A/B(LC3 A/B),p62,and autophagy protein5(ATG5).Methods Seventy specific pathogen free(SPF)Sprague-Dawley(SD)rats were randomly assigned to seven groups(n=10 in each group),including normal control,sham operation,MIRI model(model),ischemic preconditioning,Na HS,JWDSD,and JWDSD+CSE inhibitor(JWDSD+PPG)groups,and orally administered the indicated drugs for 14 d.Two hours after the last administration,the left anterior decreased branch of the coronary artery of each rat in model,Na HS,JWDSD,and JWDSD+PPG groups was ligated for 30 min and subsequently reperfused for 90 min to establish the MIRI model,and the rats in the sham operation group were only exposed to the thorax after surgery without coronary ligation.Blood samples were collected to detect H2S levels using an enzyme-linked immunosorbent assay(ELISA).Heart tissues were harvested for histopathological and immunohistochemical examination and quantitative reverse transcription polymerase chain reaction analysis of Beclin1 and ATG5 m RNA expression and Western blot analysis of Beclin1,LC3 A/B,and p62 protein expression.Results(1)The serum H2S content in model group rats was significantly reduced(P<0.01),JWDSD significantly increased the serum H2S content of model group rats(P<0.01),and the CSE inhibitor(PPG)significantly reduced H2S levels in the JWDSD group rats(P<0.01).(2)Compared with the normal control group,the myocardial tissue necrosis and cell destruction occurred in the MIRI model group,and JWDSD could alleviate the myocardial tissue necrosis of model rats,but the ameliorative effect of JWDSD could be reversed by PPG.(3)Beclin1,LC3 A/B,and p62 expression levels in the heart tissues of the model group were significantly increased(P<0.001),whereas decreased by JWDSD(P<0.05,P<0.01,and P<0.001,respectively),and the inhibitory effects of JWDSD on Beclin1,LC3 A/B,and p62 expression were partially reversed by PPG(P<0.01,P<0.05,and P<0.01,respectively).(4)The expression levels of autophagy-related genes Beclin1 and ATG5 were significantly increased in the model group(P<0.001).JWDSD clearly downregulated the expression levels of Beclin1 and ATG5(P<0.05 and P<0.001,respectively),which were reversed by PPG(P<0.001).Conclusion Our experimental data show that JWDSD can exhibit an anti-MIRI role by increasing endogenous H2S generation,and downregulating the expression of Beclin1,LC3 A/B,p62 and ATG5,which are related to inhibiting autophagy signaling.

Zhichan decoction induces differentiation of dopaminergic neurons in Parkinson's disease rats after neural stem cell transplantation

The goal of this study was to increase the dopamine content and reduce dopaminergic metabolites in the brain of Parkinson’s disease rats. Using high-performance liquid chromatography, we found that dopamine and dopaminergic metabolite（dihydroxyphenylacetic acid and homovanillic acid） content in the midbrain of Parkinson’s disease rats was increased after neural stem cell transplantation ＋ Zhichan decoction, compared with neural stem cell transplantation alone. Our genetic algorithm results show that dihydroxyphenylacetic acid and homovanillic acid levels achieve global optimization. Neural stem cell transplantation ＋ Zhichan decoction increased dihydroxyphenylacetic acid levels up to 10-fold, while transplantation alone resulted in a 3-fold increment. Homovanillic acid levels showed no apparent change. Our experimental findings show that after neural stem cell transplantation in Parkinson’s disease rats, Zhichan decoction can promote differentiation of neural stem cells into dopaminergic neurons.

Intervention of Peiyuan Huayu Decoction on the neuron damage in model rats with acute subdural hematoma

Objective:To study the intervention effect of Peiyuan Huayu Decoction on the neuron damage in model rats with acute subdural hematoma (ASDH).Methods: 160 SD rats were randomly divided into four groups, and the ASDH model rats were made by stereotactic autoblood injection, and sham operation group received craniotomy without blood injection. Sham operation group and model group were normally bred after model establishment, and 6 h after model establishment, the treatment group received intragastric administration of Peiyuan Huayu Decoction, and control group received intragastric administration of Piracetam Tablets, 1 time a day. On the 1d, 3d, 5d and 7d after model establishment, the general conditions of rats (activity, food intake and mental state) were observed, blood was collected via auricula dextra, ELISA method was used to determine peripheral plasma NSE and S100β protein contents, routine HE staining was conducted after perfusion fixation, the neurons in blood injection side of brain tissue were counted, and the neuron damage was observed.Results: 26 rats were dead in the experiment. The general conditions of sham operation group were significantly better than those of other groups, treatment group was significantly better than model group and control group on the 5d group (P<0.05), and there was no significant difference on the 1d, 3d and 7d (P>0.05);neuron count of sham operation group was basically stable, treatment group was not different from model group and control group on the 1d (P>0.05), treatment group was better than model group (P<0.05), and not different from control group (P>0.05) on the 3d, and treatment group was better than model group and control group on the 5d and 7d (P<0.05);peripheral plasma S100β protein and NSE contents of sham operation group were at lower levels, treatment group was not significantly different from model group and control group on the 1d (P>0.05), S100β protein and NSE contents decreased significantly on the 3d, and treatment group was significantly different from model group and control group (P<0.05), S100β protein and NSE contents increased on the 5d and 7d, the increase in treatment group was slower than that in model group and control group, and there was significant difference (P<0.05).Conclusion:Peiyuan Huayu Decoction has obvious protective effect on the neurons in ASDH model rats, and this effect may be based on the inhibition of secondary neuron damage.

Protective effects of Bushen Tiansui decoction on hippocampal synapses in a rat model of Alzheimer's disease

Bushen Tiansui decoction is composed of six traditional Chinese medicines：Herba Epimedii,Radix Polygoni multiflori,Plastrum testudinis,Fossilia Ossis Mastodi,Radix Polygalae,and Rhizoma Acorus tatarinowii.Because Bushen Tiansui decoction is effective against amyloid beta（Aβ） toxicity,we hypothesized that it would reduce hippocampal synaptic damage and improve cognitive function in Alzheimer＇s disease.To test this hypothesis,we used a previously established animal model of Alzheimer＇s disease,that is,microinjection of aggregated Aβ25–35 into the bilateral brain ventricles of Sprague-Dawley rats.We found that long-term（28 days） oral administration of Bushen Tiansui decoction（0.563,1.688,and 3.375 g/m L;4 m L/day） prevented synaptic loss in the hippocampus and increased the expression levels of synaptic proteins,including postsynaptic density protein 95,the N-methyl-D-aspartate receptor 2 B subunit,and Shank1.These results suggested that Bushen Tiansui decoction can protect synapses by maintaining the expression of these synaptic proteins.Bushen Tiansui decoction also ameliorated measures reflecting spatial learning and memory deficits that were observed in the Morris water maze（i.e.,increased the number of platform crossings and the amount of time spent in the target quadrant and decreased escape latency） following intraventricular injections of aggregated Aβ25–35 compared with those measures in untreated Aβ_（25–35）-injected rats.Overall,these results provided evidence that further studies on the prevention and treatment of dementia with this traditional Chinese medicine are warranted.

Buyuan Congnao decoction decreases hippocampal beta-amyloid expression in a rat model of Alzheimer's disease

A mixture of ibotenic acid and β-amyloid 1-42 was injected into the hippocampus of a rat model of Alzheimer＇s disease, followed by intragastric administration of a traditional Chinese medicine Buyuan Congnao decoction （main components included radix astragali, radix polygoni multiflori preparata, rhizoma acori talarinowii, radix polygalae, fructus alpiniae oxyphyllae, and radix glycyrrhizae preparata） and a piracetam suspension. Following treatment with traditional Chinese medicine or western medicine, β-amyloid expression decreased and neuronal morphology was normal in the rat hippocampal CA1 region, in addition to significantly shortened average latency in the Morris water navigation task. These findings suggested that compound prescription of Buyuan Congnao decoction, similar to the curative effects of piracetam, decreased hippocampal β-amyloid expression in a rat model of Alzheimer＇s disease, as well as improved learning and memory.

Tyrosine hydroxylase expression in the midbrain of Parkinson's disease model rats treated with Xifeng Dingchan decoction

This study showed that abnormal behavioral changes were greatly improved in rats displaying Parkinson＇s disease-like symptoms after intragastric administration of Xifeng Dingchan decoction at 15, 7.5, 3.75 g/kg per day. In addition, tyrosine hydroxylase mRNA expression in the substantia nigra of the midbrain was up-regulated, and tyrosine hydroxylase content in the midbrain ventral tegmentum and substantia nigra pars compacta was also increased. The effect of administration of Xifeng Dingchan decoction at 7.5 g/kg per day was similar to that of Madopar at 67.5 mg/kg per day. These results indicate that the therapeutic effect of Xifeng Dingchan decoction on Parkinson＇s disease is associated with the up-regulated protein and mRNA expression of tyrosine hydroxylase in the midbrain.

Effects of raspberry rhizome decoction on expression of C-myc and Cath-D in tumor tissues of S180 tumor-bearing mice

Objective: To investigate the effects of RRD on the expression of nuclear protein regulatory genes (C-myc) and cathepsin D (Cath-D) in tumor tissues of S180 tumor-bearing mice, and to analyze the anti-tumor effect of RRD. Methods: Forty clean ICR healthy Kunming (KM) mice were randomly divided into four groups: model group, traditional Chinese medicine group (RRD), cyclophosphamide group (CTX) and combined drug group (RRD + CTX). Ten mice in each group were subcutaneously inoculated with S180 cell suspension at the axillary position to establish the model. After 24 h, the model group was given orally 0.02 mL/g/d with normal saline;RRD group was given orally 0.4 mL/20 g/d with RRD;CTX group was given orally 0.4 mL/20 g/d with normal saline and intraperitoneally 20 mg/kg/d with CTX;RRD+CTX group was given orally 0.4 mL/20 g/d with RRD and intraperitoneally 20 mg/kg/d with CTX once a day for 10 d. The expression of C-myc and Cath-D in tumor tissues was detected by immunohistochemistry. Results: RRD could significantly decrease the expression of C-myc and Cath-D in the tumor tissues of S180 tumor-bearing mice, and there was a significant difference between RRD and model group. Conclusion: The inhibitory effect of RRD on S180 tumor-bearing mice may be related to the inhibition of the expression of C-myc and Cath-D related proteins in tumor proliferation, invasion and metastasis.

16S rRNA Technology Approach to Explore Mechanism of Qishen Decoction in Improving Nonalcoholic Fatty Liver Fibrosis

Objective: To explore the mechanism of Qishen Decoction in the treatment of nonalcoholicfatty liver fibrosis (NAFLF) by 16S rRNA technology. Methods: NAFLF rat model was established by intraperitoneal injection of carbon tetrachloride combined with high fat diet. During the modeling period, each group was given corresponding drug intervention treatment for 8 weeks. The changes of liver histopathology, serum liver function, lipid and liver fibrosis were analyzed and compared after treatment in each group. The contents of cecum end were collected and the intestinal flora was sequenced by Illumina Miseq platform. Results: Compared with the model group, Qishen Decoction could significantly improve the pathological changes of liver tissue in NALFL rats, and reduce the NAS score, oil red staining area, collagen staining area, ALT, AST, TC, TG, HA, LN, PIIINP and C-IV levels, with significant differences (P<0.05). In addition, compared with the control group, the intestinal flora abundance and diversity of the rats in the model group were significantly reduced (P<0.05), Qishen decoction could significantly increase the abundance and diversity of the intestinal flora of NAFLF rats (P<0.05), and upregulated the abundance of Bacteroidales_S24-7_group_unclassified, Bifidobacterium, Lactobacillu s, Turicibacter, Parabacteroides, Phascolarctobacterium, Lachnospiraceae_NK4A136_group, Coriobacteriaceae_UCG-002, Parasutterella, Odoribacter, Anaerostipes, Ruminococcaceae_unclassified, Allobaculum, Romboutsia, Holdemanella, and Haem ophilus, the difference was statistically significant (P<0.05). Conclusion: Qishen Decoction inhibits liver fibrosis in NAFLF rats by restore the diversity of intestinal flora and increase the abundance of probiotics in intestinal tract.

Effects of Raspberry Root decoction on cytokine level and thymus and spleen index in S180 model mice

Objective: To observe the effects of RRD on serum levels of cytokines interleukin-2 (IL-2), interleukin-10 (IL-10) and thymus and spleen index in S180 mice, and to explore the mechanism of tumor inhibition by RRD. Methods: Fifty Kunming healthy mice, half male and half female, were randomly divided into five groups: normal control group, model control group, cyclophosphamide group (CTX group), red raspberry group (RRD group) and combined administration of red raspberry and cyclophosphamide group (RRD + CTX group), with 10 mice in each group only. The other 40 mice were injected with 0.2 mLS180 tumor suspension at the right axilla to make the model experiment, except 10 mice in the normal control group. The next day, the normal control group and model control group were given intragastric administration of 0.02 mL/g/d saline, CTX group was given intragastric administration of 0.4 mL/20 g/d saline and 20 mg/kg/d CTX, RRD group was given intragastric administration of 0.4 mL/20 g/d RRD, RRD+CTX group was given intragastric administration of 0.4 mL/20 g/d RRD and 20 mg/kg/d CTX for 10 d, once a day. Serum levels of IL-2 and IL-10 were measured by ELISA, and thymus and spleen indexes were measured. Results: Red raspberry rhizome decoction could increase serum IL-2 level (P < 0.05), decrease IL-10 level (P < 0.05), increase thymus index (P < 0.05) and decrease spleen index (P < 0.05) in S180 mice. Conclusion:The anti-tumor effect of the water decoction of red raspberry rhizome may be related to the regulation of immune suppression and the improvement of immune organ function of the tumor-bearing organism.

Pharmacological mechanism of Liuwei Dihuang Decoction in treating Osteoporosis and Alzheimer’s disease based on Network Pharmacology

Objective:To predict the active components,targets of Liuwei Dihuang Decoction for the treatment of osteoporosis and Alzheimer’s disease with network pharmacology analysis.Methods:The components and targets of all the herbs of Liuwei Dihuang Decoction were obtained from ETCM database,Osteoporosis and Alzheimer’s disease targets were extracted from the GeneCards and DrugBank databases,selected based on STRING database for PPI network construction and build the network through the software of Cytoscape,Kyoto Encyclopedia of Genes and Genomes and Gene Ontology analyses were carried out by R software.Results:181 active compounds and 762 disease-related from Liuwei Dihuang Decoction,there were 144 disease-related common targets obtained from Venn Diagram.IL6,TNF,PPARA,PPARG,SRC and other 11 targets are the node protein of the whole network.In the multisystem biological reaction process such as neurotransmitter,glucose and lipid metabolism,calcium and phosphorus metabolism.Conclusion:Liuwei Dihuang pill may play a co-therapeutic role in Osteoporosis and Alzheimer’s disease by participating in the multi-system biological reaction process such as neurotransmitter,glucose and lipid metabolism,calcium and phosphorus metabolism.

Modulation of synaptic damage by Bushen Tiansui Decoction via the PI3K signaling pathway in an Alzheimer’s disease model

Objective To explore the therapeutic effect and mechanism of Bushen Tiansui Decoction(补肾填髓方,BSTSD)and its active component icariin on Alzheimer’s disease(AD).Methods(i)Animal experiments.This study conducted experiments using specific pathogen-free(SPF)grade male C57BL/6J wild-type(WT)mice and APP/PS1 double transgenic mice.The animals were divided into three groups:WT group(WT mice,n=5,receiving distilled wa-ter daily),APP/PS1 group(APP/PS1 double transgenic mice,n=5,receiving distilled water daily),and BSTSD group[APP/PS1 double transgenic mice,n=5,treated with BSTSD suspen-sion at a dosage of 27 g/(kg·d)for 90 d].Cognitive function was assessed using the Morris wa-ter maze(MWM).Post-experiment,hippocampal tissues were collected for analysis of pyra-midal cell and synaptic morphology through hematoxylin-eosin(HE)staining and transmis-sion electron microscopy(TEM).(ii)Cell experiments.The HT-22 cells were divided into con-trol group(untreated),Aβ_(25-35) group(treated with 20μmol/L Aβ_(25-35) for 24 h),icariin group(pre-treated with 20μmol/L icariin for 60 min,followed by 20μmol/L Aβ_(25-35) for an additional 24 h),and icariin+LY294002 group[treated with 20μmol/L icariin and 20μmol/L LY294002(an inhibitor of the phosphoinostitide 3-kinases(PI3K)signaling pathway)for 60 min,then exposed to 20μmol/L Aβ_(25-35) for 24 h],and cell viability was measured.Western blot was used to detect the expression levels of synapse-associated proteins[synaptophysin(SYP)and post-synaptic density-95(PSD-95)]and PI3K signaling pathway associated proteins[phosphorylat-ed(p)-PI3K/PI3K,p-protein kinase B(Akt)/Akt,and p-mechanistic target of rapamycin(mTOR)/mTOR].Results(i)Animal experiments.Compared with APP/PS1 group,BSTSD group showed that escape latency was significantly shortened(P<0.01)and the frequency of crossing the origi-nal platform was significantly increased(P<0.01).Morphological observation showed that pyramidal cells in the hippocampal CA1 region were arranged more regularly,nuclear stain-ing was uniform,and vacuole-like changes were reduced after BSTSD treatment.TEM showed that the length of synaptic active zone in BSTSD treatment group was increased com-pared with APP/PS1 group(P<0.01),and the width of synaptic gap was decreased(P<0.01).(ii)Cell experiments.Icariin had no obvious toxicity to HT-22 cells when the concentration was not more than 20μmol/L(P>0.05),and alleviated the cell viability decline induced by Aβ_(25-35)(P<0.01).Western blot results showed that compared with Aβ_(25-35) group,the ratios of p-PI3K/PI3K,p-Akt/Akt and p-mTOR/mTOR in icariin group were significantly increased(P<0.01),while the protein expression levels of SYP and PSD-95 were increased(P<0.01).These effects were blocked by LY294002(P<0.01).Conclusion BSTSD and icariin enhance cognitive function and synaptic integrity in AD mod-els and provide potential therapeutic strategies through activation of the PI3K/Akt/mTOR pathway.

安颤灵对帕金森病模型旋转行为及UPS功能影响的研究

目的探讨中药安颤灵对帕金森病(PD)大鼠模型泛素-蛋白酶体系统(UPS)功能的影响。方法采用lactacystin立体定向注射入大鼠左侧黑质致密部和黑质纹状体通路,建立PD大鼠模型。经行为测试造模成功的PD模型大鼠27只,随机分为模型组与中药组,每组10只,同时另取10只脑内注射生理盐水未出现旋转行为的大鼠作为正常对照组。正常对照组和模型组用生理盐水灌胃,中药组同时间等体积安颤灵灌胃,共5w。给药后第1、3、5周分别行阿朴吗啡(APO)诱导,观察记录其旋转行为及其他异常行为。给药结束后每组随机取6只大鼠行免疫组织化学检测,观察各组α-突触核蛋白(α-synuclein)、泛素(ubiquitin)蛋白表达。结果中药组对APO诱导的旋转行为有明显抑制作用,与模型组相比较,其旋转圈数明显减少(P<0.01)。与模型组相比,中药组α-synu-clein与硫磺素S、ubiquitin与硫磺素S荧光双标染色阳性均较弱。结论安颤灵可改善PD大鼠旋转行为及UPS的功能。

水下机器人S面控制器的改进粒子群优化

S面控制器是一种有效的水下机器人的运动控制算法,但需要人工调整参数.为了减少手工调整参数所带来的困难和误差,提出了改进的粒子群优化算法对S面控制器参数进行优化.采用动态压缩因子,加快粒子算法的收敛;引入退火算法,提高粒子算法的局部搜索能力.同时,在S面控制器中引入智能积分项,有效地减小控制器的稳态误差.最后,论述了改进粒子群算法优化S面控制参数的具体过程,并在某型水下机器人上进行了仿真试验和水池试验.试验结果表明,该算法对于水下机器人非线性控制器的参数寻优达到良好的效果,优化后的S面控制具有较快的反应速度和较小的超调.

水煎煮对石膏晶形和Ca/S值的影响

用扫描电镜和能量色散X-射线分析法对单味及白虎汤中石膏煎煮前后的晶形结构及其Ca/S值的变化进行了研究。

基于代谢网络技术的补阳还五汤对APP/PS1双转基因小鼠干预作用的研究

目的基于代谢靶标角度揭示补阳还五汤对APP/PS1双转基因小鼠的调节机制。方法选取20只7月龄APP/PS1双转基因小鼠为研究对象,建立阿尔茨海默病模型。将APP/PS1双转基因小鼠随机分为2组,即模型组和补阳还五汤组(9.26 g/kg);另选取10只健康的BALB/c小鼠作为空白组。补阳还五汤组灌胃给予补阳还五汤,空白组及模型组给予生理盐水。连续灌胃35 d后进行Morris水迷宫实验,测定小鼠学习记忆能力。在此基础上,采用高通量液相色谱质谱联用技术对各组小鼠尿液进行全面分析,通过终端代谢产物回溯疾病发展过程中的关键代谢酶或代谢通路,聚焦APP/PS1双转基因小鼠的发病机制以及补阳还五汤的作用机制。结果 Morris水迷宫实验中,与空白组比较,模型组穿越平台次数明显减少(P <0.01),补阳还五汤组穿越平台的次数显著增加(P <0.01)、在目标象限的停留时间显著增加(P <0.01)。空间定位航行实验中,补阳还五汤组较模型组的逃避潜伏期明显缩短(P <0.01)。通过进一步尿液代谢轨迹分析发现,各组小鼠尿液聚类良好,空白组和模型组间分离明显,补阳还五汤组则处于两组间。通过分组贡献较大的差异离子分析发现其主要分布在鞘脂代谢和脂肪酸代谢。给予补阳还五汤后上述代谢通路明显呈回调趋势。经高分辨质谱鉴定获取了4个代谢异常的生物标志物,分别是花生四烯酸、神经胺、L-棕榈酰肉毒碱、甘油磷脂酰胆碱。通过组学数据处理平台Metaboanalyst 4.0的在线分析可知其主要涉及两种代谢通路,分别是鞘脂代谢和花生四烯酸代谢。结论补阳还五汤对阿尔茨海默病小鼠作用明显,可有效改善其学习记忆能为。调节氨基酸代谢及能量转化可能是其调节阿尔茨海默病的关键机制。

小柴胡汤对S_(180)荷瘤小鼠红细胞免疫功能的实验研究

目的 探讨小柴胡汤对荷瘤小鼠红细胞免疫功能的影响。方法 复制S180 荷瘤小鼠模型 ,通过测定小柴胡汤不同剂量组与模型组间红细胞C3b花环结合率 (ERC -C3b)及红细胞免疫复合物IC花环率(ERC -IC)来分析小柴胡汤在红细胞免疫方面的作用。结果 小柴胡汤中、小剂量组抑瘤率高于模型组 (P<0 0 5或 0 0 1) ;与模型组比较 ,小柴胡汤中 ,小剂量组ERC -C3b降低 (P <0 0 1) ,而各剂量组ERC -IC都有升高趋势 ,但以中剂量组升高最明显 (P <0 0 5 )。结论 小柴胡汤有一定抑瘤作用 ,并对S180 荷瘤小鼠红细胞免疫功能也有抑制作用 ,说明小柴胡汤的抑瘤作用与红细胞免疫有关。

基于CiteSpace软件研究苓桂术甘汤知识图谱的可视化分析

目的:应用CiteSpace软件分析近17年苓桂术甘汤的研究情况,对相关文献进行可视化分析,总结苓桂术甘汤的研究历史、现状及未来趋势。方法:检索中国知网(CNKI)中关于苓桂术甘汤的相关文献410篇。经Refworks格式转换后,利用CiteSpace软件生成苓桂术甘汤相关文献的研究作者、机构和关键词的共现分析图谱,可视化表达。结果:通过可视化分析,发现共有50位作者被纳入,其中有8位作者发文量≥5篇;图谱共有15所机构被纳入,其中有5所机构发文量≥4篇;共有71个关键词被纳入,其中有17个关键词出现频次≥5次,以慢性心力衰竭、中医药疗法、眩晕、梅尼埃病、代谢综合征和痰饮为主。结论:直观了解了苓桂术甘汤的发展历史、应用现状及研究领域,还预测了其发展趋势,为其今后的临床应用提供了依据。

健脾活血方对大鼠胃癌前病变模型CD44V6、MLH1、MSH2表达的影响

目的:通过观察健脾活血方对胃癌前病变大鼠胃黏膜组织中CD44V6、MLH1及MSH2表达的影响,探讨健脾活血方对其干预的作用机制.方法:除正常组外,其他大鼠采用以N-甲基-N-硝基-N-亚硝基胍(N-methyl-N-nitro N-nitrosoguanidine,MNNG)为主同时配合0.3g/L雷尼替丁、400 mL/L乙醇及饥饱失常的多因素造模法建立胃癌前病变动物模型.将造模成功的40只大鼠随机分为模型组(0.9%氯化钠溶液)、胃复春组(0.86 g/kg)、健脾活血方高、中、低剂量组(32、16、8 g/kg),每组8只,每组每天给予等量(10 mL/kg)的不同药物灌胃一次,连续10 wk.实验末处死大鼠,给予相应处理后,快速免疫组织化学检测CD44V6、MLH1及MSH2表达情况.结果:模型组CD44V6表达与正常组相比明显升高(5.12±1.96 vs 0.25±0.46,P<0.01);健脾活血方高、中剂量组CD44V6表达与模型组相比均明显降低(2.25±0.71,3.25±0.31vs 5.12±1.96,P<0.01或P<0.05),低剂量组C D44V6表达与模型组比较差异无统计学意义(P>0.05);健脾活血方高剂量组CD44V6表达与胃复春组相比明显降低(2.25±0.71 vs4.62±1.19,P<0.01),中、低剂量组CD44V6表达与胃复春组比较差异无统计学意义(P>0.05).模型组MLH1、MSH2表达与正常组相比均明显降低(3.75±1.04 vs 8.00±0.926;3.62±1.69 vs 7.25±2.12,P<0.01);健脾活血方高、中、低剂量组MLH1、MSH2表达与模型组相比均明显升高(6.50±0.93,5.25±1.49,5.12±1.25 vs 3.75±1.04;6.62±2.13,6.00±1.51,5.50±1.41 vs 3.62±1.69,P<0.01或P<0.05);健脾活血方高剂量组MLH1表达与胃复春组相比明显升高(6.50±0.93 vs 4.88±1.25,P<0.05),中、低剂量组MLH1及高、中、低剂量组MSH2表达与胃复春组比较差异无统计学意义(P>0.05).结论:健脾活血方可通过降低CD44V6表达,上调MLH1、MSH2表达,减少细胞的非正常侵袭和转移,增强基因的错配修复功能,减少细胞的异常增殖和分化,发挥对大鼠胃癌前病变的治疗作用.