Serological Investigation into the Infected Genotypes of Patients with Japanese Encephalitis in the Coastal Provinces of China

Objective Genotypes(G)1,3,and 5 of the Japanese encephalitis virus(JEV)have been isolated in China,but the dominant genotype circulating in Chinese coastal areas remains unknown.We searched for G5 JEV-infected cases and attempted to elucidate which JEV genotype was most closely related to human Japanese encephalitis(JE)in the coastal provinces of China.Methods In this study,we collected serum specimens from patients with JE in three coastal provinces of China(Guangdong,Zhejiang,and Shandong)from 2018 to 2020 and conducted JEV cross-neutralization tests against G1,G3,and G5.Results Acute serum specimens from clinically reported JE cases were obtained for laboratory confirmation from hospitals in Shandong(92 patients),Zhejiang(192 patients),and Guangdong(77 patients),China,from 2018 to 2020.Seventy of the 361 serum specimens were laboratory-confirmed to be infected with JEV.Two cases were confirmed to be infected with G1 JEV,32 with G3 JEV,and two with G5 JEV.Conclusion G3 was the primary infection genotype among JE cases with a definite infection genotype,and the infection caused by G5 JEV was confirmed serologically in China.

A Study on the Implementation Path of the Blended Teaching Model in Geological Hazards Investigation and Evaluation

Geological Hazards Investigation and Evaluation is the core course of Environmental Geological Engineering,aiming to cultivate skilled talents with solid theoretical knowledge and excellent practical skills.At present,the course faces several issues,including a teaching environment disconnected from real-world work scenarios,course content that deviates from job-related tasks,a lack of digital teaching resources,and reliance on a single teaching method,leading to students’poor feedback from employers.Based on the concept of outcome-based education,the course team of Geological Hazards Investigation and Evaluation establishes a“five-step double-rotation”blended teaching model with the help of a Small Private Online Course platform.The program is designed to improve the teaching environment and expand the digitalized teaching resources in order to improve students’learning motivation,enhance learning effectiveness,and cultivate skillful talents who meet employers’satisfaction.

Analysis of Epidemiological Investigations and Responses to Human Rabies Deaths in Côte d’Ivoire, 2021

Rabies is an infectious disease that almost always leads to death as soon as the first signs appear, and is still endemic in several regions, including Côte d’Ivoire. Epidemiological investigations are carried out and response measures implemented in the event of any death from human rabies. However, the quality of these investigations does not always allow the adoption of response measures. The aim of this work was to evaluate the reports of epidemiological investigations and response actions carried out during the occurrence of human rabies deaths from 2016 to 2018. This was a cross-sectional study that took place from April to September 2021 at the National Institute of Public Hygiene and focused on human rabies deaths notified from 206 to 2018. Of sixty-one (61) human rabies deaths recorded, sixty (60) were investigated, among which fifty-nine (59) deaths had been subject to response. Most deaths occurred in rural areas (75%), and the main vector was the dog (98%). Several shortcomings were noted: response actions were not carried out promptly;joint interventions bringing together human and animal health professionals were rare, there was little communication about rabies to communities (36%), and very little vaccination of the vector, the source of the disease (12%);an almost total absence of capacity-building for local players (7%);the health districts of Abobo-Ouest, Bondoukou, Divo and Fresco, which had regularly recorded human rabies deaths, had not benefited from dog vaccination, which is supposed to break the circulation of the virus. The persistence of rabies deaths in the health districts could probably be linked to these shortcomings. Faced with these realities, it is essential to strengthen the epidemiological investigation capacities of the players involved, in order to improve the quality of investigations and ensure an effective response.

3D Model Construction and Ecological Environment Investigation on a Regional Scale Using UAV Remote Sensing

The acquisition of digital regional-scale information and ecological environmental data has high requirements for structural texture,spatial res-olution,and multiple parameter categories,which is challenging to achieve using satellite remote sensing.Considering the convenient,facilitative,and flexible characteristics of UAV(unmanned air vehicle)remote sensing tech-nology,this study selects a campus as a typical research area and uses the Pegasus D2000 equipped with a D-MSPC2000 multi-spectral camera and a CAM3000 aerial camera to acquire oblique images and multi-spectral data.Using professional software,including Context Capture,ENVI,and ArcGIS,a 3D(three-dimensional)campus model,a digital orthophoto map,and multi-spectral remote sensing map drawing are realized,and the geometric accuracy of typical feature selection is evaluated.Based on a quantitative remote sensing model,the campus ecological environment assessment is performed from the perspectives of vegetation and water body.The results presented in this study could be of great significance to the scientific management and sustainable development of regional natural resources.

Analysis of Epidemiological Investigations and Responses to Human Rabies Deaths in Côte d’Ivoire, 2021

Rabies is an infectious disease that almost always leads to death as soon as the first signs appear, and is still endemic in several regions, including Côte d’Ivoire. Epidemiological investigations are carried out and response measures implemented in the event of any death from human rabies. However, the quality of these investigations does not always allow the adoption of response measures. The aim of this work was to evaluate the reports of epidemiological investigations and response actions carried out during the occurrence of human rabies deaths from 2016 to 2018. This was a cross-sectional study that took place from April to September 2021 at the National Institute of Public Hygiene and focused on human rabies deaths notified from 206 to 2018. Of sixty-one (61) human rabies deaths recorded, sixty (60) were investigated, among which fifty-nine (59) deaths had been subject to response. Most deaths occurred in rural areas (75%), and the main vector was the dog (98%). Several shortcomings were noted: response actions were not carried out promptly;joint interventions bringing together human and animal health professionals were rare, there was little communication about rabies to communities (36%), and very little vaccination of the vector, the source of the disease (12%);an almost total absence of capacity-building for local players (7%);the health districts of Abobo-Ouest, Bondoukou, Divo and Fresco, which had regularly recorded human rabies deaths, had not benefited from dog vaccination, which is supposed to break the circulation of the virus. The persistence of rabies deaths in the health districts could probably be linked to these shortcomings. Faced with these realities, it is essential to strengthen the epidemiological investigation capacities of the players involved, in order to improve the quality of investigations and ensure an effective response.

Investigation,Analysis,and Countermeasures of College Teachers'Psychological Pressure

Objective:This paper aims to explore the investigation,analysis,and countermeasures of the psychological pressure of college teachers.Method:From March 2023 to May 2023,3266 college teachers in our city were selected as the research objects,and the sources of their psychological stress were analyzed through a questionnaire survey.Results:In this paper,there were 500 cases of education and teaching pressure accounting for 15.31%,600 cases of research pressure accounting for 18.37%,1000 cases of professional title evaluation pressure accounting for 30.62%,and 1266 cases of life pressure accounting for 35.70%.Conclusion:The psychological pressure on teachers in colleges and universities in our city is relatively high,and this paper puts forward targeted countermeasures for this problem.

Application of 3D GPR attribute technology in archaeological investigations

Ground penetrating radar （GPR） attribute technology has been applied to many aspects in recent years but there are very few examples in the field of archaeology. Especially how can we extract effective attributes from the two- or three-dimensional radar data so that we can map and describe numerous archaeological targets in a large cultural site？ In this paper, we applied GPR attribute technology to investigate the ancient Nanzhao castle-site in Tengchong, Yunnan Province. In order to get better archaeological target （the ancient wall, the ancient kiln site, and the ancient tomb） analysis and description, we collated the GPR data by collected standardization and then put them to the seismic data processing and interpretation workstation. The data was processed, including a variety of GPR attribute extraction, analysis, and optimization and combined with the archaeological drilling data. We choose the RMS Amplitude, Average Peak Amplitude, Instantaneous Phase, and Maximum Peak Time to interpret three archaeological targets. By comparative analysis, we have clarified that we should use different attributes to interpret different archaeological targets and the results of attribute analysis after horizon tracking is much better than the results based on a time slice.

Investigation of the epidemiology,pathogenicity and immunogenicity of Bordetella bronchiseptica isolated from cats and dogs in China from 2021 to 2023

Bordetella bronchiseptica(Bb)is recognized as a leading cause of respiratory diseases in dogs and cats.However,epidemiological data on Bb in dogs and cats in China are still limited,and there is no commercially available vaccine.Live vaccines containing Bb that are widely used abroad are generally efective but can establish latency and potentially reactivate to cause illness in some immunodefcient vaccinated recipients,raising safety concerns.In this study,34 canine-derived and two feline-derived Bb strains were isolated from 1809 canine and 113 feline nasopharyngeal swab samples collected from eight provinces in China from 2021 to 2023.The PCR results showed that the percentage of positive Bb was 22.94%(441/1922),and more than 90%of the Bb isolates had four virulence factor-encoding genes(VFGs),namely,fhaB,prn,betA and dnt.All the isolated strains displayed a multidrug-resistant phenotype.The virulence of 10 Bb strains isolated from dogs with respiratory symptoms was tested in mice,and we found that eight isolates were highly virulent.Furthermore,the eight Bb isolates with high virulence were inactivated and intramuscularly injected into mice,and three Bb strains(WH1218,WH1203 and WH1224)with the best protective efcacy were selected.Dogs immunized with these three strains exhibited strong protection against challenge with the Bb feld strain WH1218.Ultimately,the WH1218 strain with the greatest protection in dogs was selected as the vaccine candidate.Dogs and cats that received a vaccine containing 109 CFU of the inactivated WH1218 strain showed complete protection against challenge with the Bb feld strain WH1218.This study revealed that Bb is an important pathogen that causes respiratory diseases in domestic dogs and cats in China,and all the isolates exhibited multidrug resistance.The present work contributes to the current understanding of the prevalence,antimicrobial resistance,and virulence genes of Bb in domestic dogs and cats.Additionally,our results suggest that the WH1218 strain is a promising candidate safe and efcacious inactivated Bb vaccine.

Experimental investigation on coal pore-fracture variation and fractal characteristics synergistically affected by solvents for improving clean gas extraction

Chemical solvents instead of pure water being as hydraulic fracturing fluid could effectively increase permeability and improve clean methane extraction efficiency.However,pore-fracture variation features of lean coal synergistically affected by solvents have not been fully understood.Ultrasonic testing,nuclear magnetic resonance analysis,liquid phase mass spectrometry was adopted to comprehensively analyze pore-fracture change characteristics of lean coal treated by combined solvent(NMP and CS_(2)).Meanwhile,quantitative characterization of above changing properties was conducted using geometric fractal theory.Relationship model between permeability,fractal dimension and porosity were established.Results indicate that the end face fractures of coal are well developed after CS2and combined solvent treatments,of which,end face box-counting fractal dimensions range from 1.1227 to 1.4767.Maximum decreases in ultrasonic longitudinal wave velocity of coal affected by NMP,CS_(2)and combined solvent are 2.700%,20.521%,22.454%,respectively.Solvent treatments could lead to increasing amount of both mesopores and macropores.Decrease ratio of fractal dimension Dsis 0.259%–2.159%,while permeability increases ratio of NMR ranges from 0.1904 to 6.4486.Meanwhile,combined solvent could dissolve coal polar and non-polar small molecules and expand flow space.Results could provide reference for solvent selection and parameter optimization of permeability-enhancement technology.

Serological Investigation on Mycoplasma Oviovipneumoniae in Qinghai Province

[ Objective] To investigate the prevalence of Mycoplasma oviovipneurnoniae in Qinghai Province. [ Method] With positive indirect hemagglutination test kit for detecting antibodies against Mycoplasma oviovipneumoniae, 965 sheep sera and 208 goat sera were collected in Qinghai Province from 2006 to 2008 and detected. [ Result ] The positive rate of sheep sera and goat sera was 30.4% and 19.7%, respectively. The posi- tive rate of sheep sera collected from different regions ranged from 19.7% to 40.2%, and that of goat sere ranged from 6.6% to 22.2%. In addition, the incidence in winter and spring was higher than that in summer and autumn. [ Conclusion ] The infection rate of Mycoplasrna ovipneumoniae should be higher in Qinghai region than in other western regions, so it is necessary to strengthen the prevention and control of this disease.

Numerical investigation of friction-heating-pressurization and its control parameters in the shear band of high-speed landslides

High-speed sliding often leads to catastrophic landslides,many of which,in the initial sliding phase before disintegration,experience a friction-induced thermal pressurization effect in the bottom shear band,accelerating the movement of the overlying sliding mass.To quantitatively investigate this complex multiphysical phenomenon,we established a set of equations that describe the variations in temperature and excess pore pressure within the shear band,as well as the conservation of momentum equation for the overlying sliding mass.With a simplified landslide model,we investigated the variations of temperature and excess pore pressure within the shear band and their impacts on the velocity of the overlying sliding mass.On this basis,we studied the impact of seven key parameters on the maximum temperature and excess pore pressure in the shear band,as well as the impact on the velocity of the overlying sliding mass.The simulation results of the standard model show that the temperature and excess pore pressure in the shear band are significantly higher than those in the adjacent areas,and reach the maximum values in the center.Within a few seconds after the start,the maximum excess pore pressure in the shear zone is close to the initial stress,and the shear strength loss rate exceeds 90%.The thermal pressurization mechanism significantly increases the velocity of the overlying sliding mass.The results of parameter sensitivity analysis show that the thermal expansion coefficient has the most significant impact on the temperature and excess pore pressure in the shear band,and the sliding surface dip angle has the most significant impact on the velocity of the overlying sliding mass.The results of this study are of great significance for clarifying the mechanism of thermal pressurization-induced high-speed sliding.

Experimental investigation on the permeability of gap-graded soil due to horizontal suffusion considering boundary effect

The boundary condition is a crucial factor affecting the permeability variation due to suffusion.An experimental investigation on the permeability of gap-graded soil due to horizontal suffusion considering the boundary effect is conducted,where the hydraulic head difference(DH)varies,and the boundary includes non-loss and soil-loss conditions.Soil samples are filled into seven soil storerooms connected in turn.After evaluation,the variation in content of fine sand(ΔR_(f))and the hydraulic conductivity of soils in each storeroom(C_(i))are analyzed.In the non-loss test,the soil sample filling area is divided into runoff,transited,and accumulated areas according to the negative or positive ΔR_(f) values.ΔR_(f) increases from negative to positive along the seepage path,and Ci decreases from runoff area to transited area and then rebounds in accumulated area.In the soil-loss test,all soil sample filling areas belong to the runoff area,where the gentle-loss,strengthened-loss,and alleviated-loss parts are further divided.ΔR_(f) decreases from the gentle-loss part to the strengthened-loss part and then rebounds in the alleviated-loss part,and C_(i) increases and then decreases along the seepage path.The relationship between ΔR_(f) and Ci is different with the boundary condition.Ci exponentially decreases with ΔR_(f) in the non-loss test and increases with ΔR_(f) generally in the soil-loss test.

Experimental investigation of dynamic characteristics of leaching tubing for solution mining of salt cavern carbon and energy storage

Salt caverns are extensively utilized for storing various substances such as fossil energy,hydrogen,compressed air,nuclear waste,and industrial solid waste.In China,when the salt cavern is leached through single-well water solution mining with oil as a cushion,engineering challenges arise with the leaching tubing,leading to issues like damage and instability.These problems significantly hinder the progress of cavern construction and the control of cavern shape.The primary cause of this is the flowinduced vibration instability of leaching tubing within a confined space,which results in severe bending or damage to the tubing.This study presents a model experimental investigation on the dynamic characteristics of leaching tubing using a self-developed liquid-solid coupling physical model experiment apparatus.The experiment utilizes a silicone-rubber pipe(SRP)and a polycarbonate pipe(PCP)to examine the effects of various factors on the dynamic stability of cantilevered pipes conveying fluid.These factors include external space constraint,flexural rigidity,medium outside the pipe,overhanging length,and end conditions.The experiments reveal four dynamic response phenomena:water hammer,static buckling,chaotic motion,and flutter instability.The study further demonstrates that the length of the external space constraint has a direct impact on the flutter critical flow velocity of the cantilevered pipe conveying fluid.Additionally,the flutter critical flow velocity is influenced by the end conditions and different external media.

Logical stochastic resonance in a cross-bifurcation non-smooth system

This paper investigates logical stochastic resonance(LSR)in a cross-bifurcation non-smooth system driven by Gaussian colored noise.In this system,a bifurcation parameter triggers a transition between monostability,bistability and tristability.By using Novikov's theorem and the unified colored noise approximation method,the approximate Fokker-Planck equation is obtained.Then we derive the generalized potential function and the transition rates to analyze the LSR phenomenon using numerical simulations.We simulate the logic operation of the system in the bistable and tristable regions respectively.We assess the impact of Gaussian colored noise on the LSR and discover that the reliability of the logic response depends on the noise strength and the bifurcation parameter.Furthermore,it is found that the bistable region has a more extensive parameter range to produce reliable logic operation compared with the tristable region,since the tristable region is more sensitive to noise than the bistable one.

Systematic investigation of Radix Salviae for treating diabetic peripheral neuropathy disease based on network Pharmacology

BACKGROUND Diabetic peripheral neuropathy(DPN)is a debilitating complication of diabetes mellitus with limited available treatment options.Radix Salviae,a traditional Chinese herb,has shown promise in treating DPN,but its therapeutic mech-anisms have not been systematically investigated.AIM Radix Salviae(Danshen in pinin),a traditional Chinese medicine(TCM),is widely used to treat DPN in China.However,the mechanism through which Radix Salviae treats DPN remains unclear.Therefore,we aimed to explore the mechanism of action of Radix Salviae against DPN using network pharmacology.METHODS The active ingredients and target genes of Radix Salviae were screened using the TCM pharmacology database and analysis platform.The genes associated with DPN were obtained from the Gene Cards and OMIM databases,a drug-com-position-target-disease network was constructed,and a protein–protein inter-action network was subsequently constructed to screen the main targets.Gene Ontology(GO)functional annotation and pathway enrichment analysis were performed via the Kyoto Encyclopedia of Genes and Genomes(KEGG)using Bioconductor.RESULTS A total of 56 effective components,108 targets and 4581 DPN-related target genes of Radix Salviae were screened.Intervention with Radix Salviae for DPN mainly involved 81 target genes.The top 30 major targets were selected for enrichment analysis of GO and KEGG pathways.CONCLUSION These results suggested that Radix Salviae could treat DPN by regulating the AGE-RAGE signaling pathway and the PI3K-Akt signaling pathway.Therefore,Danshen may affect DPN by regulating inflammation and apoptosis.

Investigation and Analysis of Main Diseases of Landscape Plants in the Main Urban Area of Lu'an City

To figure out the disease occurrence of landscape plants in the main urban area of Lu'an City,the author investigated the disease occurrence of landscape plants in park green space,residential green space,unit attached green space and main road in the area under administration.The survey results showed that there were 29 species of urban landscape plant diseases,mainly powdery mildew and spot diseases.According to the characteristics of the diseases,the causes and problems of the diseases were analyzed,and the corresponding prevention and control measures were put forward.

A Survey of Crime Scene Investigation Image Retrieval Using Deep Learning

Crime scene investigation(CSI)image is key evidence carrier during criminal investiga-tion,in which CSI image retrieval can assist the public police to obtain criminal clues.Moreover,with the rapid development of deep learning,data-driven paradigm has become the mainstreammethod of CSI image feature extraction and representation,and in this process,datasets provideeffective support for CSI retrieval performance.However,there is a lack of systematic research onCSI image retrieval methods and datasets.Therefore,we present an overview of the existing worksabout one-class and multi-class CSI image retrieval based on deep learning.According to theresearch,based on their technical functionalities and implementation methods,CSI image retrievalis roughly classified into five categories:feature representation,metric learning,generative adversar-ial networks,autoencoder networks and attention networks.Furthermore,We analyzed the remain-ing challenges and discussed future work directions in this field.

Auto-parametric resonance of a continuous-beam-bridge model under two-point periodic excitation:an experimental investigation and stability analysis

The auto-parametric resonance of a continuous-beam bridge model subjected to a two-point periodic excitation is experimentally and numerically investigated in this study.An auto-parametric resonance experiment of the test model is conducted to observe and measure the auto-parametric resonance of a continuous beam under a two-point excitation on columns.The parametric vibration equation is established for the test model using the finite-element method.The auto-parametric resonance stability of the structure is analyzed by using Newmark's method and the energy-growth exponent method.The effects of the phase difference of the two-point excitation on the stability boundaries of auto-parametric resonance are studied for the test model.Compared with the experiment,the numerical instability predictions of auto-parametric resonance are consistent with the test phenomena,and the numerical stability boundaries of auto-parametric resonance agree with the experimental ones.For a continuous beam bridge,when the ratio of multipoint excitation frequency(applied to the columns)to natural frequency of the continuous girder is approximately equal to 2,the continuous beam may undergo a strong auto-parametric resonance.Combined with the present experiment and analysis,a hypothesis of Volgograd Bridge's serpentine vibration is discussed.

Psychological Study on the Influence of Physical Exercise on College Students’ Anxiety

This study aims to explore the impact of physical exercise on the anxiety level of college students.We collected physical exercise data and anxiety self-assessment results from college students of different grades,genders,and ages from multiple universities in Guangdong Province through a questionnaire survey.The study used standardized scales to evaluate participants’physical exercise types,frequencies,intensities,and anxiety levels.The data analysis results showed that there is a significant negative correlation between college students’physical exercise and anxiety level,that is,the more frequent and intense the physical exercise,the lower the anxiety level.In addition,there are differences in the effect of different types of physical exercises on anxiety relief,with aerobic exercises such as running and swimming showing more significant anxiety-relieving effects.The research results provide valuable references for psychological health education and anxiety intervention strategies in universities and suggest promoting physical exercise to effectively alleviate college students’anxiety.

A Case of Investigation and Diagnosis of Immune Thrombocytopenic Purpura After Vaccination of COVID-19 Inactivated Vaccine

Objective:Analyze the relationship between inoculating one case of the COVID-19 inactivated vaccine(Vero cell)and immune thrombocytopenic purpura to provide a reference for the standardized handling of adverse events following immunization.Methods:According to the"National Monitoring Program for Suspected Adverse Reactions to Vaccinations,"an on-site investigation,data collection and analysis,expert group diagnosis,and medical association assessment were conducted on a case of immune thrombocytopenic purpura in District A of Chongqing after vaccination with the inactivated COVID-19 vaccine.The assessment report was delivered to the three relevant parties,the case was reviewed,and the experience was summarized.Results:The investigation and diagnosis by the district-level vaccination abnormal reaction expert group concluded that the disease that occurred after vaccination with the COVID-19 inactivated vaccine was secondary immune thrombocytopenic purpura,an abnormal reaction to the vaccination.The medical damage was classified as Level II Grade B.The vaccine production enterprise raised objections to this conclusion.After re-assessment by the municipal-level medical association,the conclusion was consistent with that of the district-level medical association.The vaccine production enterprise did not raise any further objections.Conclusion:Through active collaboration among district and municipal-level medical associations,disease control institutions,and vaccination units,the recipients have been promptly and effectively treated,providing financial support for their subsequent treatment and safeguarding their rights.The investigation and disposal procedures for adverse events following immunization in Chongqing are clear,and the mechanism is sound.It is necessary to continue strengthening the monitoring of adverse events following immunization according to the existing plan and to ensure timely and standardized handling.Simultaneously,it is crucial to strengthen vaccine management and vaccination management.