Objective:To explore the effect of Qingre Quzhuo Capsule(QRQZ)in the treatment of nonalcoholic steato-hepatitis(NASH)and to explore its mechanism from the perspective of inhibiting ferroptosis.Methods:60 C57BL/6J mice...Objective:To explore the effect of Qingre Quzhuo Capsule(QRQZ)in the treatment of nonalcoholic steato-hepatitis(NASH)and to explore its mechanism from the perspective of inhibiting ferroptosis.Methods:60 C57BL/6J mice were randomly divided into 6 groups:Control,model,ferroptosis inhibitor(Fer-1,10 mg/kg,gavage),low-dose QRQZ(QRQZL,482 mg/kg,gavage),medium-dose QRQZ(QRQZM,964 mg/kg,gavage),and high-dose QRQZ(QRQZH,1929 mg/kg,gavage).The control group was given normal diet,and the other experimental groups were given MCD diet.The whole administration process lasted for 6 weeks.The body weight of mice was counted every week.After 6 weeks,the blood of mice was collected,and the serum was separated to determine ALT and AST.The liver of mice was weighed and fixed.The same part was stained with HE and Oil Red O to observe the pathological changes of liver tissue.The levels of TC,TG,total iron,GSH and GSSG in liver tissue were measured by kit.The expression of Gpx4 mRNA was detected by RT-qPCR,and the expression of FTH1,FTL and GPX4 protein was detected by Western blotting.Results:Compared with the model group,after treatment with Fer-1 and QRQZ,the body weight and liver index of NASH mice increased,the liver tissue lesions were alleviated,TC,TG,ALT and AST were improved,the liver tissue iron level decreased significantly,the relative levels of FTH1 and FTL proteins in-creased significantly,GSH/GSSG increased significantly,and the expression of Gpx4 mRNA and GPX4 protein increased significantly.Conclusion:QRQZ alleviates liver injury in NASH mice by promoting the expression of GSH/GPX4 antioxidant system and inhibiting ferroptosis.展开更多
Background:To observe the clinical effect of Qingre Quzhuo capsule in the treatment of non-alcoholic fatty liver disease.Methods:90 patients with non-alcoholic fatty liver disease in our hospital from January 2021 to ...Background:To observe the clinical effect of Qingre Quzhuo capsule in the treatment of non-alcoholic fatty liver disease.Methods:90 patients with non-alcoholic fatty liver disease in our hospital from January 2021 to January 2022 were randomly divided into observation group and control group,45 cases in each group.All patients received routine lifestyle intervention and the control group received oral polyene phosphatidylcholine capsules 2 capsules/time(456 mg),three times daily;45 cases in the observation group were given Qingre Quzhuo capsule(Jiyao Z2017040,0.5 g/grain)2.5 g/time,3 times/day,for 12 weeks.The clinical efficacy,liver function,liver transient elastography controlled attenuation index value,lipid metabolism,oxidation and antioxidant indexes were compared between the two groups after treatment.Results:The observation indexes of the two groups after treatment were better than those before treatment(P<0.05).After treatment,the total effective rate of the observation group was higher than that of the control group.Lipid metabolism indexes and oxidation indexes were lower than those in the control group.All antioxidant indexes were higher than control group.The decrease of liver transient elastography controlled attenuation index value was better than that of the control group and the difference was statistically significant(P<0.05).There was no significant difference in the decrease of alanine aminotransferase between the observation group and the control group after treatment.The decrease of r-glutamyltransferase was statistically significant(P<0.05).Conclusion:Qingre Quzhuo capsule in the treatment of non-alcoholic fatty liver disease can improve liver function,regulate lipid metabolism indexes of patients,improve the antioxidant capacity of liver cells and improve the controlled attenuation index value of liver transient elastography.In addition,it is superior to polyene phosphatidylcholine in regulating lipid metabolism,antioxidant capacity and controlled attenuation index value of patients with non-alcoholic fatty liver disease which is safe and worthy of promotion.展开更多
Objective:To elucidate the effects of Huangqin Qingre Chubi capsule(HQC)on bone metabolism and serum TLR4 and NF-kB in rats with rheumatoid arthritis(RA),and to provide experimental bases for the treatment of RA with ...Objective:To elucidate the effects of Huangqin Qingre Chubi capsule(HQC)on bone metabolism and serum TLR4 and NF-kB in rats with rheumatoid arthritis(RA),and to provide experimental bases for the treatment of RA with HQC.Methods:A total of 40 SD rats were randomly divided into the normal group,model group,western medicine treatment group,and HQC group,with 10 rats in each group,and the RA model was induced by complete adjuvant in all groups except the normal group.After successful modeling,they were treated for 4 weeks to compare the degree of joint swelling;meanwhile,micro-CT was used to evaluate bone microstructural parameters,and changes in serum TLR4 and NF-kB expression levels were detected by ELISA.Results:There was no statistical significance in comparing the degree of joint swelling among the model group,western medicine treatment group and HQC group(P>0.05);Micro-CT results showed that bone microstructural parameters deteriorated in the osteoporosis model group compared with the normal group.Both western medicine and traditional Chinese medicine HQC could improve the bone microjunction of RA rats,and the HQC group was better than the western medicine treatment in improving the number of bone trabeculae(2.58±0.19)·mm^(-1);the serum results showed that RA was accompanied by the up-regulation of the expression of TLR4 and NF-kB.Both western drugs and HQC down-regulated the high expression of serum TLR4 and NF-kB in osteoporotic rats,and the down-regulation of serum TLR4(9.90±0.55)ng·ml^(-1)and NF-kB(350.29±3.14)ng·L^(-1)by HQC was more obvious.Conclusion:Chinese herbal medicine HQC can significantly improve the bone microstructure of RA rats,and its effect is better than that of western medicine in some aspects.The mechanism of action of HQC is related to the inhibition of the activation of the TLR4/NF-kB pathway,and this study provides an experimental basis for the further development and utilization of HQC.展开更多
基金Su Xiuhai National Famous Traditional Chinese Medicine Expert Inheritance Studio[No.Guozhong Pharmaceutical Renjiao Letter (2022)No.75]。
文摘Objective:To explore the effect of Qingre Quzhuo Capsule(QRQZ)in the treatment of nonalcoholic steato-hepatitis(NASH)and to explore its mechanism from the perspective of inhibiting ferroptosis.Methods:60 C57BL/6J mice were randomly divided into 6 groups:Control,model,ferroptosis inhibitor(Fer-1,10 mg/kg,gavage),low-dose QRQZ(QRQZL,482 mg/kg,gavage),medium-dose QRQZ(QRQZM,964 mg/kg,gavage),and high-dose QRQZ(QRQZH,1929 mg/kg,gavage).The control group was given normal diet,and the other experimental groups were given MCD diet.The whole administration process lasted for 6 weeks.The body weight of mice was counted every week.After 6 weeks,the blood of mice was collected,and the serum was separated to determine ALT and AST.The liver of mice was weighed and fixed.The same part was stained with HE and Oil Red O to observe the pathological changes of liver tissue.The levels of TC,TG,total iron,GSH and GSSG in liver tissue were measured by kit.The expression of Gpx4 mRNA was detected by RT-qPCR,and the expression of FTH1,FTL and GPX4 protein was detected by Western blotting.Results:Compared with the model group,after treatment with Fer-1 and QRQZ,the body weight and liver index of NASH mice increased,the liver tissue lesions were alleviated,TC,TG,ALT and AST were improved,the liver tissue iron level decreased significantly,the relative levels of FTH1 and FTL proteins in-creased significantly,GSH/GSSG increased significantly,and the expression of Gpx4 mRNA and GPX4 protein increased significantly.Conclusion:QRQZ alleviates liver injury in NASH mice by promoting the expression of GSH/GPX4 antioxidant system and inhibiting ferroptosis.
基金Project source:Hebei Provincial Planning Commission(No.20171185)Hebei Provincial Administration of Traditional Chinese Medicine(No.2018479,No.2021308)Yuan-Song Wang Heritage Studio of Famous Traditional Chinese Medicine in Hebei.
文摘Background:To observe the clinical effect of Qingre Quzhuo capsule in the treatment of non-alcoholic fatty liver disease.Methods:90 patients with non-alcoholic fatty liver disease in our hospital from January 2021 to January 2022 were randomly divided into observation group and control group,45 cases in each group.All patients received routine lifestyle intervention and the control group received oral polyene phosphatidylcholine capsules 2 capsules/time(456 mg),three times daily;45 cases in the observation group were given Qingre Quzhuo capsule(Jiyao Z2017040,0.5 g/grain)2.5 g/time,3 times/day,for 12 weeks.The clinical efficacy,liver function,liver transient elastography controlled attenuation index value,lipid metabolism,oxidation and antioxidant indexes were compared between the two groups after treatment.Results:The observation indexes of the two groups after treatment were better than those before treatment(P<0.05).After treatment,the total effective rate of the observation group was higher than that of the control group.Lipid metabolism indexes and oxidation indexes were lower than those in the control group.All antioxidant indexes were higher than control group.The decrease of liver transient elastography controlled attenuation index value was better than that of the control group and the difference was statistically significant(P<0.05).There was no significant difference in the decrease of alanine aminotransferase between the observation group and the control group after treatment.The decrease of r-glutamyltransferase was statistically significant(P<0.05).Conclusion:Qingre Quzhuo capsule in the treatment of non-alcoholic fatty liver disease can improve liver function,regulate lipid metabolism indexes of patients,improve the antioxidant capacity of liver cells and improve the controlled attenuation index value of liver transient elastography.In addition,it is superior to polyene phosphatidylcholine in regulating lipid metabolism,antioxidant capacity and controlled attenuation index value of patients with non-alcoholic fatty liver disease which is safe and worthy of promotion.
文摘Objective:To elucidate the effects of Huangqin Qingre Chubi capsule(HQC)on bone metabolism and serum TLR4 and NF-kB in rats with rheumatoid arthritis(RA),and to provide experimental bases for the treatment of RA with HQC.Methods:A total of 40 SD rats were randomly divided into the normal group,model group,western medicine treatment group,and HQC group,with 10 rats in each group,and the RA model was induced by complete adjuvant in all groups except the normal group.After successful modeling,they were treated for 4 weeks to compare the degree of joint swelling;meanwhile,micro-CT was used to evaluate bone microstructural parameters,and changes in serum TLR4 and NF-kB expression levels were detected by ELISA.Results:There was no statistical significance in comparing the degree of joint swelling among the model group,western medicine treatment group and HQC group(P>0.05);Micro-CT results showed that bone microstructural parameters deteriorated in the osteoporosis model group compared with the normal group.Both western medicine and traditional Chinese medicine HQC could improve the bone microjunction of RA rats,and the HQC group was better than the western medicine treatment in improving the number of bone trabeculae(2.58±0.19)·mm^(-1);the serum results showed that RA was accompanied by the up-regulation of the expression of TLR4 and NF-kB.Both western drugs and HQC down-regulated the high expression of serum TLR4 and NF-kB in osteoporotic rats,and the down-regulation of serum TLR4(9.90±0.55)ng·ml^(-1)and NF-kB(350.29±3.14)ng·L^(-1)by HQC was more obvious.Conclusion:Chinese herbal medicine HQC can significantly improve the bone microstructure of RA rats,and its effect is better than that of western medicine in some aspects.The mechanism of action of HQC is related to the inhibition of the activation of the TLR4/NF-kB pathway,and this study provides an experimental basis for the further development and utilization of HQC.
