Objective:To observe the sustained expression of transient outward potassium channel related proteins at the end of the treatment and 30 days after the end of the treatment in rats,and to explore the sustained curativ...Objective:To observe the sustained expression of transient outward potassium channel related proteins at the end of the treatment and 30 days after the end of the treatment in rats,and to explore the sustained curative effect and mechanism of acupuncture combined with Xijingtongmai decoction in rats with myocardial infarction.Methods:Twenty of 130 male SD rats were random extracted as the control group,and the rest were used to establish myocardial infarction by fed with high-fat diet and then injected with isoproterenol.According to ECG,80 rats were successfully established.Then they were randomly divided into model group,acupuncture combined with Chinese medicine group,acupuncture group and Western medicine group.The content of bFGF protein was measured by ELISA.The protein contents of Kv1.4,Kv4.2,Kv4.3 and KChIP2 were measured by Western blot.Results:At the end of treatment,compared with the model group,the protein contents of Kv1.4,Kv4.2,Kv4.3,KChIP2 and bFGF in each treatment group increased,and the increase was most significant in the acupuncture combined with Chinese medicine group(P<0.05).At the end of treatment,compared with the model group,the protein contents of Kv1.4,Kv4.2,Kv4.3,KChIP2 and bFGF in each treatment group increased,and the increase was most significant in the acupuncture combined with Chinese medicine group(P<0.05).Compared with the treatment group at the end of treatment,the expression of Kv1.4,Kv4.2,Kv4.3,KChIP2 and bFGF protein in each treatment group 30 days after the end of treatment decreased slightly(P<0.05),but still higher than that of the model group at this time(P<0.05).The combination of acupuncture and Chinese medicine group decreased the least of them(P<0.05).Conclusion:The results showed that acupuncture combined with xijingtongmai decoction had a sustained good effect.Its sustained action mechanism may be achieved by continuously increasing the protein content of Kv1.4,Kv4.2,Kv4.3,KChIP2 and bFGF through transient outward potassium channel.展开更多
Objective Study on the effect of NF-κB in ox-LDL injured HUVEC-304 and the intervention with the serum of Tongmai Decoction. Methods Tongmai Decoction herbage-contained serum was made by the serum pharmacology method...Objective Study on the effect of NF-κB in ox-LDL injured HUVEC-304 and the intervention with the serum of Tongmai Decoction. Methods Tongmai Decoction herbage-contained serum was made by the serum pharmacology methods, then the HUVEC-304 cells were divided into 5 groups and incubated for 24 h: 1) normal group;2) fetal calf serum group; 3) ox-LDL group; 4) simvastatin group; 5) Tongmai Decoction group. RT-PCR was used to determine the mRNA of NF-κB in the cells, Western Blot Assay was used to determine the protein content of NF-κB in the cells. Results 1) Compared with the cells in normal group and serum control group , the endothelial cells in ox-LDL group are seriously injured, while those in Tongmai Decoction group grow better; 2) Compared with the cells in normal group and fetal calf serum group, the expressions of NF-κB gene and protein are up-regulated in ox-LDL group(P＜0.05, P＜0.01); 3) Compared with the cells in ox-LDL group, the expression of NF-κB gene and protein in Tongmai Decoction group are significantly down-regulated, there is no difference between Tongmai Decoction group and simvastatin group(P＞0.05). Conclusion Tongmai Decoction can protect the injured endothelial cells, up-regulate the expressions of NF-κB gene and protein content.展开更多
Objective: To investigate the curative effect of Tongmai Huoxue Decoction combined with warm needling on the treatment of High altitude polycythemia (HAPC), and to analyze the changes of hemorheology and antioxidant a...Objective: To investigate the curative effect of Tongmai Huoxue Decoction combined with warm needling on the treatment of High altitude polycythemia (HAPC), and to analyze the changes of hemorheology and antioxidant ability before and after treatment. Methods: A total of 90 HAPC patients were randomly divided into control group and observation group. The control group was treated with low molecular weight dextran and captopril. On the basis of this, the observation group was supplemented with Tongmai Huoxue Decoction combined with warm needling. Hemorheology and antioxidant markers were measured before and after treatment in all subjects. Results: Before treatment, there were no significant differences in the hemorheology indexes between the control group and the observation group. After treatment, the two groups of hemorheology related indicators were significantly lower than those before treatment, including whole blood low shear reduction viscosity, whole blood high shear reduction viscosity, plasma viscosity, Hb, Hct and RBC. The levels of the observation group were significantly lower than that of the control group. Before treatment, there was no significant difference in antioxidant indexes between the control group and the observation group. After treatment, the levels of serum superoxide dismutase (SOD) and glutathione (GSH) in the two groups were higher than those in the pre-treatment, while the content of malondialdehyde (MDA) was lower than that before treatment. The levels of serum SOD and GSH in the observation group were higher than those in the control group, and the MDA content was lower than that in the control group. Conclusion: Routine treatment of Western medicine combined with Tongmai Huoxue Decoction joint warm needling method may reduce hemoglobin, improve hemorheology and improve antioxidant ability in HAPC patients, and it may be one of the potential effective drugs for adjuvant therapy of HAPC.展开更多
基金Key project of Liaoning provincial science and technology foundation(No.20180530079)。
文摘Objective:To observe the sustained expression of transient outward potassium channel related proteins at the end of the treatment and 30 days after the end of the treatment in rats,and to explore the sustained curative effect and mechanism of acupuncture combined with Xijingtongmai decoction in rats with myocardial infarction.Methods:Twenty of 130 male SD rats were random extracted as the control group,and the rest were used to establish myocardial infarction by fed with high-fat diet and then injected with isoproterenol.According to ECG,80 rats were successfully established.Then they were randomly divided into model group,acupuncture combined with Chinese medicine group,acupuncture group and Western medicine group.The content of bFGF protein was measured by ELISA.The protein contents of Kv1.4,Kv4.2,Kv4.3 and KChIP2 were measured by Western blot.Results:At the end of treatment,compared with the model group,the protein contents of Kv1.4,Kv4.2,Kv4.3,KChIP2 and bFGF in each treatment group increased,and the increase was most significant in the acupuncture combined with Chinese medicine group(P<0.05).At the end of treatment,compared with the model group,the protein contents of Kv1.4,Kv4.2,Kv4.3,KChIP2 and bFGF in each treatment group increased,and the increase was most significant in the acupuncture combined with Chinese medicine group(P<0.05).Compared with the treatment group at the end of treatment,the expression of Kv1.4,Kv4.2,Kv4.3,KChIP2 and bFGF protein in each treatment group 30 days after the end of treatment decreased slightly(P<0.05),but still higher than that of the model group at this time(P<0.05).The combination of acupuncture and Chinese medicine group decreased the least of them(P<0.05).Conclusion:The results showed that acupuncture combined with xijingtongmai decoction had a sustained good effect.Its sustained action mechanism may be achieved by continuously increasing the protein content of Kv1.4,Kv4.2,Kv4.3,KChIP2 and bFGF through transient outward potassium channel.
基金Project (02jjy3034) supported by the Nature Science Foundation of Hunan Province
文摘Objective Study on the effect of NF-κB in ox-LDL injured HUVEC-304 and the intervention with the serum of Tongmai Decoction. Methods Tongmai Decoction herbage-contained serum was made by the serum pharmacology methods, then the HUVEC-304 cells were divided into 5 groups and incubated for 24 h: 1) normal group;2) fetal calf serum group; 3) ox-LDL group; 4) simvastatin group; 5) Tongmai Decoction group. RT-PCR was used to determine the mRNA of NF-κB in the cells, Western Blot Assay was used to determine the protein content of NF-κB in the cells. Results 1) Compared with the cells in normal group and serum control group , the endothelial cells in ox-LDL group are seriously injured, while those in Tongmai Decoction group grow better; 2) Compared with the cells in normal group and fetal calf serum group, the expressions of NF-κB gene and protein are up-regulated in ox-LDL group(P＜0.05, P＜0.01); 3) Compared with the cells in ox-LDL group, the expression of NF-κB gene and protein in Tongmai Decoction group are significantly down-regulated, there is no difference between Tongmai Decoction group and simvastatin group(P＞0.05). Conclusion Tongmai Decoction can protect the injured endothelial cells, up-regulate the expressions of NF-κB gene and protein content.
文摘Objective: To investigate the curative effect of Tongmai Huoxue Decoction combined with warm needling on the treatment of High altitude polycythemia (HAPC), and to analyze the changes of hemorheology and antioxidant ability before and after treatment. Methods: A total of 90 HAPC patients were randomly divided into control group and observation group. The control group was treated with low molecular weight dextran and captopril. On the basis of this, the observation group was supplemented with Tongmai Huoxue Decoction combined with warm needling. Hemorheology and antioxidant markers were measured before and after treatment in all subjects. Results: Before treatment, there were no significant differences in the hemorheology indexes between the control group and the observation group. After treatment, the two groups of hemorheology related indicators were significantly lower than those before treatment, including whole blood low shear reduction viscosity, whole blood high shear reduction viscosity, plasma viscosity, Hb, Hct and RBC. The levels of the observation group were significantly lower than that of the control group. Before treatment, there was no significant difference in antioxidant indexes between the control group and the observation group. After treatment, the levels of serum superoxide dismutase (SOD) and glutathione (GSH) in the two groups were higher than those in the pre-treatment, while the content of malondialdehyde (MDA) was lower than that before treatment. The levels of serum SOD and GSH in the observation group were higher than those in the control group, and the MDA content was lower than that in the control group. Conclusion: Routine treatment of Western medicine combined with Tongmai Huoxue Decoction joint warm needling method may reduce hemoglobin, improve hemorheology and improve antioxidant ability in HAPC patients, and it may be one of the potential effective drugs for adjuvant therapy of HAPC.
