Transcriptome profiling and RXR gene family identification reveals the molecular mechanism of rapid aging after spawning of cuttlefish Sepiella japonica

Sepiella japonica is a worldwide marine cuttlefish species of high economic value.S.japonica routinely modifying behaviors in reproductive life,such as rapid aging until death after spawning,has been recognized in artificial breeding.However,reproductive behavior at the level of genes is rarely reported,thus,the research on the genetic basis of behavior,reproduction,and artificial breeding was limited.We applied RNA-seq in different stages of reproduction to investigate the reason of rapid aging after spawning,pre-maturity,pre-spawning after maturity,and post-spawning.The retinoid X receptor(RXR)gene family in S.japonica was identified,and 1343–1452 differentially expressed genes(DEGs)in all 3 stages of reproductive life were identified from pairwise m RNA comparisons.Furthermore,through the GO term and KEGG analysis,S.japonica could handle neuronal development and network formation before maturity and have a functional degradation of neural communication,signal transduction,vision,and gene expression after spawning.Eight Sj RXRαs have been identified and they played different roles in growth development or reproduction.Therefore,the regulation of several channels and receptors is the intrinsic molecular mechanism of rapid aging after spawning in S.japonica.This study revealed the survival strategy and provided fundamental data on the level of genes for understanding the reproductive behavior and the reproduction of S.japonica.

Experimental study on the mechanical properties and consolidation mechanism of microbial grouted backfill

Backfill mining technology is the practice of returning waste materials underground for both disposal and geotechnical stability,however,a challenge with current technologies is that they commonly require cement-based binders which have a relatively high environmental impact.Finding alternatives to cement-based binders can improve environmental performance and this paper proposes microbial grouted backfill(MGB)as a potential solution.In this paper,the effects of the cementation solution concentration(CSC),volume ratio of bacterial solution to cementation solution(VRBC),particle sizes of the aggregates,and the number of grouting batches on the mechanical properties of MGB are studied.The experimental results show that MGB strength increased,up to a peak value,as CSC was increased,before decreasing as CSC was increased further.The results also show that MGB strength increased,up to a peak value,as VRBC decreased,before decreasing as the VRBC was decreased further.The peak strength was achieved at a CSC of 2 mol/L and a VRBC of 1:9.The strength of the MGB also increased as the number of grouting batches increased.Graded MGB samples showed the highest UCS,25.12 MPa,at particle sizes of 0.2 to 0.8 mm,while full(non-graded)MGB samples displayed mean UCS values ranging from1.56 MPa when the maximum particle size was 0.2 mm,up to 13 MPa when the maximum particle size was 1.2 mm.MGB samples are consolidated by the calcium carbonate that is precipitated during microbial metabolism,and the strength of MGB increases linearly as calcium carbonate content increases.The calcium carbonate minerals produced in MGB materials are primarily calcite,with secondary amounts of vaterite.

Consolidation mechanism of gold concentrates containing sulfur and carbon during oxygen-enriched air roasting

Consolidation in calcines is a common problem in the oxygen-enriched air roasting of refractory gold concentrates containing sulfur and carbon when the initial temperature is greater than 600°C. To determine the phases that caused consolidation, gold concentrates were roasted under different conditions and the calcines were mainly detected by X-ray diffraction (XRD). The possible underlying mechanism was then studied through comparisons of the XRD patterns of different calcines. The results indicated that the generation of calcium magnesium silicate, iron-doped calcium aluminosilicate, and calcium aluminate caused the consolidation. Furthermore, an enriched oxygen atmosphere accelerated the oxidation reaction and the emitted heat increased the local temperature in calcines. The local temperature was inferred to have increased to the generation temperature zone of the corresponding liquid phases. Oxidation of the pyrite and decomposition of the dolomite and muscovite mainly occurred at the initial stage of oxygen-enriched air roasting. Calcium was confirmed to be essential to the consolidation process. © 2017, University of Science and Technology Beijing and Springer-Verlag Berlin Heidelberg.

Effect of consolidation parameters on mechanical properties of Cu-based bulk amorphous alloy consolidated by hot pressing

Cu50Zr40Ti10 bulk amorphous alloys were fabricated by hot pressing gas-atomized Cu50Zr40Ti10 amorphous powder under different consolidation conditions without vacuum and inert gas protection. The consolidation conditions of the Cu50Zr40Ti10 amorphous powder were investigated based on an L9（34） orthogonal design. The compression strength and strain limit of the Cu50Zr40Ti10 bulk amorphous alloys can reach up to 1090.4 MPa and 11.9 %, respectively. The consolidation pressure significantly influences the strain limit and compression strength of the compact. But the mechanical properties are not significantly influenced by the consolidation temperature. In addition, the preforming pressure significantly influences not the compression strength but the strain limit. The optimum consolidation condition for the Cu50Zr40Ti10 amorphous powder is first precompacted under the pressure of 150 MPa, and then consolidated under the pressure of 450 MPa and the temperature of 380 °C.

Genome wide investigation of Hsf gene family in Phoebe bournei:identification,evolution,and expression after abiotic stresses

Heat shock transcription factors(Hsfs)have important roles during plant growth and development and responses to abiotic stresses.The identification and func-tion of Hsf genes have been thoroughly studied in various herbaceous plant species,but not woody species,especially Phoebe bournei,an endangered,unique species in China.In this study,17 members of the Hsf gene family were identi-fied from P.bournei using bioinformatic methods.Phyloge-netic analysis indicated that PbHsf genes were grouped into three subfamilies:A,B,and C.Conserved motifs,three-dimensional structure,and physicochemical properties of the PbHsf proteins were also analyzed.The structure of the PbHsf genes varied in the number of exons and introns.Pre-diction of cis-acting elements in the promoter region indi-cated that PbHsf genes are likely involved in responses to plant hormones and stresses.A collinearity analysis dem-onstrated that expansions of the PbHsf gene family mainly take place via segmental duplication.The expression levels of PbHsf genes varied across different plant tissues.On the basis of the expression profiles of five representative PbHsf genes during heat,cold,salt,and drought stress,PbHsf pro-teins seem to have multiple functions depending on the type of abiotic stress.This systematic,genome-wide investigation of PbHsf genes in P.bournei and their expression patterns provides valuable insights and information for further func-tional dissection of Hsf proteins in this endangered,unique species.

Examining the pathway and mechanism of comprehensive land consolidation through the lens of rural neo-endogenous development

Land consolidation(LC) stands as a globally recognized strategy for rural development. In China, it has evolved towards comprehensive land consolidation(CLC) to support the rural revitalization initiative. However, there are ongoing challenges in understanding CLC's specific pathway and mechanism, particularly its role in stimulating rural endogenous development. This study aims to investigate the localization process of international experiences, examine the pathway of CLC, and scrutinize its mechanism in rural development from a novel perspective of neo-endogenous development. Field research and semi-structured interviews were conducted in Nanzhanglou village, renowned for its early adoption of CLC practices inspired by German experiences since 1988. Overall, key findings underscore the advantages of CLC in spatial restructuring, industrial development, and human capital enhancement in rural areas. Additionally, international experiences emerge as crucial exogenous forces, primarily by knowledge embedding, which catalyzes rural neo-endogenous development via the “resource-engagement-identity-endogenous” mechanism. Collectively, by introducing a neo-endogenous theoretical framework, this study offers valuable insights into the CLC implementation in China and beyond, and emphasizes the positive impact of knowledge embedding as an exogenous force in promoting rural neo-endogenous development to address existing research gaps. Recommendations for sustainable rural development involve enhancing rural planning practicality, governance capacity, and local leadership, while prioritizing agricultural modernization and increasing investments in education and vocational training to ensure that villagers benefit from industrial development.

Exploration on Ecological Compensation Mechanism of Family Farm

Family farm is a new type of agricultural operation,and the research on ecological compensation for its production behavior is conducive to consolidating the basic position of agriculture and protecting agricultural resources and environment.By summarizing and drawing lessons from the domestic research results in related fields,the family farm is selected as the object,and the opportunity cost method is used to analyze the setting of the ecological compensation standard of the family farm.The basic framework of ecological compensation mechanism of family farm is constructed from three aspects:ecological compensation responsibility determination mechanism;compensation object establishment mechanism;compensation standard determination and mode selection mechanism.The operation mechanism of ecological compensation of family farm is analyzed from three aspects:farmer;farm production;resources and environment.The results show that the family farms'pursuit of agricultural products and ecological services is the response mechanism of ecological compensation for their production behavior.On the basis of this,this paper puts forward some measures and suggestions to ensure the ecological compensation mechanism of family farms from three aspects:perfecting the land use system;widening the compensation channels;constructing the green GDP accounting.

Experiences of implementing a coping mechanism for the elderly who face chronic diseases while living with the family: a phenomenology study

Objective: Physical and psychological stress causes harm to the health status of the elderly with chronic diseases. This study aimed to understand coping mechanisms of the elderly with chronic conditions who live with their family. Methods: This study was conducted using a descriptive phenomenology method from the experience of 13 older adults with chronic disease. The study processes were interviewed, tape recorded, transcribed, and explored from the transcripts using Colaizzi’s descriptive phenomenological method. The steps of the descriptive phenomenology process are bracketing, intuiting, analyzing, and describing. Results: The coping mechanisms used by the elderly with chronic diseases are (1) the behavioral focus coping ways by doing sports, and physical activities;(2) Focus on spirituality has been implemented by fasting, chanting, dhikr, and prayer;(3) The cognitive focus by working on hobbies or habitual activities and helping each other;(4) The social interaction focus was by interacting with friends, family, and neighbors. Conclusions: This shows that elderly adults with disease conditions try to adapt various forms of coping mechanisms, which positively affects their psychological state. Families which have elderly with chronic diseases are expected to provide nurturing and psychological support to them so that the elderly can consistently apply coping mechanisms to overcome and tackle chronic diseases. Understanding the coping mechanism implementation of the elderly who have chronic diseases by their family can guide health specialists in designing psychological and spiritual approach interventions.

Requirement of Family Enterprises of Feed and Veterinary Drugs for Improving Professional Manager Mechanism and Recommendations

In the development of family enterprises of feed and veterinary drugs in China,there are many problems,such as lack of organic integration of human capital and social human capital.This paper introduced development thread of family enterprises of feed and veterinary drugs in China,analyzed theories and practical development requirement for improving professional manager mechanism,and came up with recommendations including establishing proper employing system,incentive and restraint mechanism,corporate culture,and professional manager market.

Microstructure and properties of submicron-scale TiC particle reinforced titanium matrix composites prepared by shock wave consolidation

Submicron-scale TiC particle reinforced titanium matrix composites(TMCs) were prepared by shock wave consolidation technique at detonation speed of 2 5005 000 m/s. The microstructures were studied by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The compressive strength and hardness values of the composites were also determined. The results show that the composites have higher compressive yield strength and hardness values than hot-rolled pure titanium. Twins in the microstructure of TMCs show that titanium particles undergo plastic deformation during consolidation process. The fine grains with size less than 1 μm often locate in the boundaries among the titanium particles. TiC particles seem to keep unchanged during the consolidation. These bring about the increase in strength and hardness for the composites. The detonation speed of 3 200 m/s is proper parameter for compacting powder in the present work.

Gully erosion and expansion mechanisms in loess tablelands and the scientific basis of gully consolidation and tableland protection

Gully erosion is serious in the tableland area of the Loess Plateau due to high-intensity human activities and extreme rainfall, which cause serious soil loss and an increasing tableland shrinkage rate. Severe gully erosion has exerted a notable negative impact on local agriculture, human life and socioeconomic development. In recent decades, progress has been made in soil and water conservation with the goal of reducing soil erosion and protecting loess tableland, but basic research on gully consolidation and tableland protection(GCTP) is lacking, especially regarding the mechanisms of gully erosion and expansion in loess tableland under the interactive impacts of hydrodynamics and human activities. In addition, there is a lack of a deep understanding of the underlying mechanisms of soil-water disasters and controlling factors of unreasonable GCTP projects.Currently, the problems of headcut erosion and tableland fragmentation remain serious. Based on this situation, the Dongzhi tableland, the largest tableland on the Loess Plateau, was adopted as an example, and we studied gully erosion and expansion mechanisms in the loess tableland and the scientific basis of GCTP projects. We obtained a series of novel findings, including the following:(1) vertical joints are widely developed in loess and impose a controlling effect on tableland edge erosion;(2) rapid urbanization and road network expansion intensify headcut erosion and are the main reasons for severe erosion and tableland shrinkage in the Dongzhi tableland;and(3) unreasonable drainage of surface runoff and a rise in the groundwater level are the key factors affecting GCTP project stability. Moreover, the mechanisms and modes of erosion disasters in the project driven by these two factors were explained. The systematic remediation idea of retention, storage, drainage and consolidation for the GCTP project was introduced, and the core is water control, which emphasizes the combination of soil and water conservation and geohazard prevention measures. As a systematic remediation project, GCTP in loess tableland requires multidisciplinary and multimethod approaches and multiple measures involving ecology, soil and water conservation, geology and engineering to ensure project feasibility and sustainability.

Sediment consolidation settlement of Chengbei Sea area in the northern Huanghe River subaqueous delta,China

One of the most important factors controlling the morphology of the modem Huanghe （Yellow） River delta is consolidation settlement, which is impacted by fast deposition, high water content, and low density of seafloor sediment. Consolidation settlement of the Huanghe River subaqueous delta was studied based on field data, laboratory experiments on 12 drill holes, and the one-dimensional consolidation theory. Results show that vertical sediment characteristics varied greatly in the rapidly forming sedimentary bodies of the modem Huanghe River subaqueous delta. Sediments in the upper parts of drill holes were coarser than those in the deeper parts, and other physical and mechanical properties changed accordingly. On the basis of the one-dimensional consolidation theory and drilling depth, the final consolidation settlement of drill holes was between 0.6 m and 2.8 m, and the mean settlement of unit depth was at 1.5-3.5 cm/m. It takes about 15-20 years for the consolidation degree to reach 90% and the average sedimentation rate within the overlying 50 m strata was at 5 cm/a to 12 cm/a. This study helps to forecast the final consolidation settlement and settlement rate of the modem Huanghe River subaqueous delta, which provides key geotechnical information for marine engineers.

磷矿粉球团冷固结成型试验及机械性能的影响因素

针对磷矿粉矿无法直接入炉冶炼黄磷的问题,本文采用磷矿粉冷固结成型方法来制备磷矿粉球团,研究黏结剂用量、水分添加量、成型压力和烧结温度对磷矿粉球团落下强度和抗压强度的影响。研究结果表明:球团的抗压强度和落下强度随黏结剂用量的增加而升高,随成型压力的增加呈现先升高后降低,随水分质量分数的增加呈现先升高后降低,抗压强度随着烧结温度的升高而升高;在黏结剂用量为0.4%、水分质量分数为8%、成型压力为11 MPa条件下制备的磷矿粉生球团的抗压强度可达148.46 N/P,落下强度达46次/(0.5 m);在1200℃下烧结2 h后,磷矿球团的抗压强度可达966 N/P,且经跌落破碎后无粉化现象。本文旨在弥补富磷矿块矿资源的不足,并为进一步促进磷矿粉矿在黄磷生产中的应用提供参考。

融合石膏组分和微纳米结构“双基因”调控的铁尾矿粉无熟料固结体力学性能

为了探究超细铁尾矿粉颗粒表面非晶态SiO_(2)和Al_(2)O_(3)在石灰-石膏体系中的水化固化机理及调控因素,本工作考察了石膏掺量和铁尾矿粉中微纳米级颗粒含量之间的匹配对固结体强度的影响,利用XRD、TG-DSC、SEM和化学滴定法测试分析了固结体试样中水化产物的种类、形貌及含量的变化,研究了这些变化对固结体强度的影响及作用机理。结果表明,超细铁尾矿粉中小于1.096μm的微纳米颗粒含量显著影响着铁尾矿粉在碱溶液中Si和Al元素的溶出行为,固结体强度随着这些微纳米颗粒含量的增加而提高。固结体中的主要水化产物是铝掺杂的水化硅酸钙(C-(A)-S-H)和钙矾石(AFt)。石膏掺量与铁尾矿粉中微纳米颗粒含量的最佳匹配值对固结体的强度具有显著影响,并且微纳米颗粒含量越高,对应的石膏最佳掺量越大。石膏组分保证了AFt的生成和稳定,同时也加速了铁尾矿粉颗粒表面非晶态成分的水化。本工作探明了固结材料中最佳石膏掺量与微纳米铁尾矿粉颗粒含量的关联关系及其机理,证实了微纳米级颗粒是无熟料固结体结构中的基本活性单元,石膏的掺入对微纳米颗粒的水化起到调控作用,水化后的微纳米颗粒演变为固结体的基本结构单元,这种组分和结构的“双基因”调控机制影响着固结体的力学性能。

miR-192对结直肠癌细胞系增殖、迁移和侵袭能力的影响

目的 探究微小RNA-192(miR-192)对结直肠癌(CC)细胞系增殖、迁移和侵袭能力的影响。方法 分别设立A组(SW1116 CC细胞转染生理盐水)、B组(SW1116 CC细胞转染miR-192模拟物)及C组(SW1116 CC细胞转染miR-192抑制剂)。分别以MTT法检测各组细胞增殖情况,以流式细胞术检测各组细胞凋亡情况,以划痕实验检测细胞迁移能力,以Transwell实验检测细胞侵袭能力。采用实时荧光定量聚合酶链式反应(qRT-PCR)检测各组细胞miR-192及WNT家族成员2B(WNT2B)mRNA表达水平。结果 B组SW1116 CC细胞存活率、单克隆形成数目分别为(57.32±6.19)%、(284.59±15.08)个,均低于A组的(76.21±8.23)%、(601.47±23.16)个与C组的(89.52±10.62)%、(2 150.68±34.79)个,而凋亡率为(20.52±2.52)%,高于A组的(13.78±1.62)%与C组的(11.62±1.41)%;C组SW1116 CC细胞存活率、单克隆形成数目均高于A组,而凋亡率低于A组(均P<0.05)。B组SW1116 CC细胞划痕宽度为(785.10±46.18)mm,高于A组的(601.32±33.21)mm与C组的(326.99±17.48)mm,而C组划痕宽度低于A组,B组穿膜细胞数为(624.67±19.05)个,少于A组的(875.23±27.30)个与C组的(1 204.17±34.59)个,且C组穿膜细胞数多于A组(均P<0.05)。B组SW1116 CC细胞miR-192 mRNA相对表达水平为(3.01±0.26),相较于A组的(1.87±0.20)及C组的(0.97±0.23)均更高,且C组miR-192 mRNA相对表达水平低于A组,B组WNT2B mRNA表达水平为(2.16±0.26),相较于A组的(4.11±0.50)与C组的(6.08±0.72)均更低,且C组WNT2B mRNA表达水平高于A组(均P<0.05)。结论 miR-192可抑制CC的恶性演进,其主要作用机制之一可能与调控WNT2B表达有关。

泥炭质土非线性固结理论研究

在泥炭质土软基固结过程中,孔隙比和渗透系数会随固结时间增加逐渐变小,导致按Terzaghi一维固结理论计算的软基固结值与实测值存在较大误差,故以滇池湖相沉积泥炭质土为研究对象,进行固结-渗透联合试验,研究泥炭质土e-σ′,k-σ′之间规律,从而得到e-lgσ′,lgk-lgσ′模型,然后引入这两个模型推导出适用于泥炭质土的一维非线性固结理论,并求解出渗压指数C_(k)=1,C_(k)=2两种不同条件下的解析解。根据所取泥炭质土的C_(k)不同,当C_(k)=1时,一维非线性固结微分方程的解析解与Terzaghi一维固结方程解析解比较,仅竖向固结系数存在差异;当C_(k)=2时,一维非线性固结微分方程难以直接得到解析解,可引入激活函数得到非线性固结微分方程的一个特解。研究发现,C_(k)=1时,泥炭质土固结过程中Terzaghi一维固结理论预测沉降速率比实测快,固结完成所需时间短;推导的一维非线性固结理论预测的各时间段固结沉降值和实测值接近,预测固结完成所需的时间误差小,且上覆荷载越大St-t关系曲线与实测值越接近,推导的一维非线性固结理论预测结果越精确。研究结果可为泥炭质土类软基提供一种更为精确的固结计算方法。

从“法制”到“法治”:改革开放以来我国家庭教育政策的演进逻辑与推进路径

家庭教育政策是规范与支持家庭教育发展的重要工具,是推进我国家庭家教家风建设的重要手段。改革开放以来,我国家庭教育政策经历了以边缘性探索为中心的萌芽发展阶段、以系统性规划为抓手的改革发展阶段和以体系化建设为重点的深入发展阶段。在此过程中,其动力机制、价值取向、实施过程保障及话语规则均有其独特的演进逻辑。家庭教育法治化是我国教育治理现代化的必然要求,在未来的发展中我国家庭教育从“法制”走向“法治”,可采取以下推进路径:形成强化基础建设、优质均衡发展的政策动力机制,秉持兼顾个体与社会、协调多元利益的政策价值取向,深化加强顶层设计、完善组织建设的政策实施过程保障,体现深入了解民情、广泛反映民意的政策话语规则。

家庭代际学习:模式、要素与作用机理

家庭代际学习是代际学习在家庭场域中的具体应用,它贯穿家庭的各个生命阶段,是真正意义上的终身学习实践。当前家庭代际学习存在开放共享、基于尊重的自上而下、基于尊重的自下而上和开放非共享四种典型模式,其与家庭的构成要素,即家庭结构、互动层次、学习目标与内容密切相关。从家庭结构来看,复合家庭、直系家庭、核心家庭三种家庭结构相对传统和稳定,任何家庭代际学习模式都可能发生;不完全家庭多表现为开放共享或非共享家庭代际学习模式。从互动层次来看,基于尊重的自上而下模式和基于尊重的自下而上模式均以家庭内学习为主;开放共享模式和开放非共享模式均存在个体参与形式的家庭外学习,而以家庭为单位参与的家庭外学习只发生在开放共享模式中。从学习目标与内容来看,无论哪种家庭代际学习模式,都包含知识、技能与意识提升等学习目标,以及知识传授、技能传递与意识形态建设等学习内容。家庭代际学习的有效发生受家庭凝聚力、灵活性和沟通三大因素影响。因此,推进家庭代际学习,一是要注重回归家庭深度联结,激发家庭代际学习原生动力;二是要明晰多元构成要素,选择适合的家庭代际学习模式;三是要重视有效调节机制,形成家庭学习可持续性生态。

全域土地综合整治支撑乡村振兴用地要素保障:机理与策略

土地是乡村振兴最根本的基础性要素保障,全域土地综合整治把区域国土空间视为一个有机整体,着眼于“山水林田湖草村”等全要素的整体保护、系统修复和综合治理,对于改变“零散无序”的农村土地利用、支撑乡村振兴用地要素保障具有积极的现实意义。本文深入剖析了全域土地综合整治保障乡村振兴用地要素的内在机理,具体表现为:以“山水林田湖草村”的统筹策应乡村振兴目标的系统性、以工程建设和制度创新的融合保障用地供给的有效性、以破解现实难题提升用地保障的针对性。并提出了相应的策略建议:一是坚持系统思维,实现空间重构;二是坚持政府主导,确保统筹力度;三是坚持村民主体,激发内生动力;四是坚持因地制宜,突出整治实效。

政社力量帮扶事实无人抚养儿童家庭的联动机制研究——以上海市为例

现代家庭的儿童保护功能趋向弱化甚至缺失,本研究关注地方政府和社会力量在为事实无人抚养儿童家庭提供福利服务方面如何开展联动工作并优化协同路径,通过分析相关政策文件,并在政策实施地开展参与观察、半结构访谈和焦点小组访谈总结地方帮扶经验,借助生态位理论视角对事实无人抚养儿童家庭关爱服务生态系统进行全面梳理。研究发现,不同层级的政府部门、群团组织和社会组织三大福利供给主体分别构成了三条生态链主线,通过“政社合作”和“群社协同”两条上行和下行联动路径吸纳各类社会资源并转化为社会服务。其中,专业社会组织处于关键生态位,是各类联动工作的中枢核心;发现报告是联动工作的关键环节,但仍需进一步优化协作机制。未来应强化专业社会组织的培育与发展,实施家庭增权服务,管控伦理风险,推进社会力量的有序联动和社会资源的广泛吸纳,并完善相关儿童福利政策监督反馈机制,以增进事实无人抚养儿童家庭的福祉。