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Effects of long-term partial substitution of inorganic fertilizer with pig manure and/or straw on nitrogen fractions and microbiological properties in greenhouse vegetable soils
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作者 Shuo Yuan Ruonan Li +5 位作者 Yinjie Zhang Hao'an Luan Jiwei Tang Liying Wang Hongjie Ji Shaowen Huang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第6期2083-2098,共16页
Partial substitution of inorganic fertilizers with organic amendments is an important agricultural management practice.An 11-year field experiment(22 cropping periods)was carried out to analyze the impacts of differen... Partial substitution of inorganic fertilizers with organic amendments is an important agricultural management practice.An 11-year field experiment(22 cropping periods)was carried out to analyze the impacts of different partial substitution treatments on crop yields and the transformation of nitrogen fractions in greenhouse vegetable soil.Four treatments with equal N,P_(2)O_(5),and K_(2)O inputs were selected,including complete inorganic fertilizer N(CN),50%inorganic fertilizer N plus 50%pig manure N(CPN),50%inorganic fertilizer N plus 25%pig manure N and 25%corn straw N(CPSN),and 50%inorganic fertilizer N plus 50%corn straw N(CSN).Organic substitution treatments tended to increase crop yields since the 6th cropping period compared to the CN treatment.From the 8th to the 22nd cropping periods,the highest yields were observed in the CPSN treatment where yields were 7.5-11.1%greater than in CN treatment.After 11-year fertilization,compared to CN,organic substitution treatments significantly increased the concentrations of NO_(3)^(-)-N,NH_(4)^(+)-N,acid hydrolysis ammonium-N(AHAN),amino acid-N(AAN),amino sugar-N(ASN),and acid hydrolysis unknown-N(AHUN)in soil by 45.0-69.4,32.8-58.1,49.3-66.6,62.0-69.5,34.5-100.3,and 109.2-172.9%,respectively.Redundancy analysis indicated that soil C/N and OC concentration significantly affected the distribution of N fractions.The highest concentrations of NO_(3)^(-)-N,AHAN,AAN,AHUN were found in the CPSN treatment.Organic substitution treatments increased the activities ofβ-glucosidase,β-cellobiosidase,N-acetyl-glucosamidase,L-aminopeptidase,and phosphatase in the soil.Organic substitution treatments reduced vector length and increased vector angle,indicating alleviation of constraints of C and N on soil microorganisms.Organic substitution treatments increased the total concentrations of phospholipid fatty acids(PLFAs)in the soil by 109.9-205.3%,and increased the relative abundance of G^(+)bacteria and fungi taxa,but decreased the relative abundance of G-bacteria,total bacteria,and actinomycetes.Overall,long-term organic substitution management increased soil OC concentration,C/N,and the microbial population,the latter in turn positively influenced soil enzyme activity.Enhanced microorganism numbers and enzyme activity enhanced soil N sequestration by transforming inorganic N to acid hydrolysis-N(AHN),and enhanced soil N supply capacity by activating non-acid hydrolysis-N(NAHN)to AHN,thus improving vegetable yield.Application of inorganic fertilizer,manure,and straw was a more effective fertilization model for achieving sustainable greenhouse vegetable production than application of inorganic fertilizer alone. 展开更多
关键词 organic substitution management greenhouse vegetable production N fractions microbial community composition extracellular enzyme activity enzyme stoichiometry coefficients
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Cosmetic or Dietary Vegetable Oils Sampled in the Cameroonian Market May Not Expose Consumers to Lipid Oxidation Products Generating Oxidative Stress and Inflammation
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作者 Ferdinand Kouoh Elombo Erika Van Damme +5 位作者 Clara Delepine David Depraetere Ludovic Chaveriat Paul Lunga Keilah Nico Fréderic Njayou Patrick Martin 《American Journal of Plant Sciences》 CAS 2024年第3期193-202,共10页
Vegetable oils are a source of energy, essential fatty acids, antioxidants and fat-soluble vitamins useful for human health care and development. These oils also contribute to organoleptic quality of their products’ ... Vegetable oils are a source of energy, essential fatty acids, antioxidants and fat-soluble vitamins useful for human health care and development. These oils also contribute to organoleptic quality of their products’ derivatives. However, their chemical and physical properties can be modified by the mode of their extraction, storage and distribution. These modifications might negatively affect the nutritional quality of the oils. The goals of this study were to: sample different vegetable oils for cosmetic or dietary use marketed in Cameroon, and verify purity and oxidation states of each kind of oil through determination of its acidity, iodine, peroxide, saponification, refractive indexes and the conformity of the labeling. The carotene content, the level of polar components and specific absorbance were also determined. As the result, six oils namely palm, palm kernel, coconut, black cumin, peanut and shea butter were collected. Apart from labeling, chemicals and physicals parameters analyzed were generally in accordance with the Cameroonian and Codex Alimentarius standard. This study suggests that vegetable oils sampled in the Cameroonian market may not expose consumers to lipid oxidation products generating pathological oxidative stress and inflammation. However, efforts in application of existing standard need to be done as far as labeling are concerned. 展开更多
关键词 vegetable Oils Quality Control Labeling Compliance Lipid Oxidation Oxidative Pathology
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Detection of Adenovirus in Fresh Fruit, Vegetables, Wastewater and Manure from Irrigated Farms in Ouagadougou, Burkina Faso
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作者 Kuan Abdoulaye Traore Madou Sanou +3 位作者 Jean Bienvenue Ouoba Bruno Lalidia Ouoba Pierre Roques Nicolas Barro 《Food and Nutrition Sciences》 CAS 2024年第7期644-662,共19页
Enteric viral pathogens are responsible for numerous epidemics associated with the consumption of fresh fruit and vegetable, whether raw or minimally processed. The aim of the present study was to assess agricultural ... Enteric viral pathogens are responsible for numerous epidemics associated with the consumption of fresh fruit and vegetable, whether raw or minimally processed. The aim of the present study was to assess agricultural practices and the presence of adenovirus (AdV) in fruits and vegetables, manure and irrigation wastewater sampled in the urban and peri-urban perimeters of Ouagadougou. A total of 286 samples including 30 lettuces, 42 tomatoes, 30 carrots, 30 strawberries, 74 manures and 80 wastewater samples were collected from four market garden sites in and around Ouagadougou. Nested PCR was performed with specific primers to detect adenoviruses (AdVs). A face-to-face survey was carried out using a questionnaire on market garden production practices. Overall, adenoviruses prevalence was 5.9% [IC95, 3.2% - 8.7%] in all samples analyzed. It was specifically 7.14% (3/42) from tomatoes, 6.7% (2/30) from lettuces, 20% (6/30) on strawberries and 7.5% (6/80) in irrigation water. The survey showed that irrigation water came from untreated sources (dam, well, canal) and then 52% of farms used untreated manure. No farms have implemented measures to limit access by domestic and wild animals. This work shows the presence of human adenoviruses in surface irrigation water and fresh produce, which is of concern when fresh produce is consumed raw. To reduce the public health risks associated with consuming these foods, it is essential to follow good hygiene and cultivation practices. 展开更多
关键词 ADENOVIRUS Raw Fruits and vegetables Nested PCR WASTEWATER MANURE OUAGADOUGOU
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Optimizing Household Wastes (Rice, Vegetables, and Fruit) as an Environmentally Friendly Electricity Generator
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作者 Deni Ainur Rokhim Isma Yanti Vitarisma +2 位作者 Sumari Sumari Yudhi Utomo Muhammad Roy Asrori 《Journal of Renewable Materials》 EI CAS 2024年第2期275-284,共10页
The high consumption of electricity and issues related to fossil energy have triggered an increase in energy prices and the scarcity of fossil resources.Consequently,many researchers are seeking alternative energy sou... The high consumption of electricity and issues related to fossil energy have triggered an increase in energy prices and the scarcity of fossil resources.Consequently,many researchers are seeking alternative energy sources.One potential technology,the Microbial Fuel Cell(MFC)based on rice,vegetable,and fruit wastes,can convert chemical energy into electrical energy.This study aims to determine the potency of rice,vegetable,and fruit waste assisted by Cu/Mg electrodes as a generator of electricity.The method used was a laboratory experiment,including the following steps:electrode preparation,waste sample preparation,incubation of the waste samples,construction of a reactor using rice,vegetable,and fruit waste as a source of electricity,and testing.The tests included measuring electrical conductivity,electric current,voltage,current density,and power density.Based on the test results,the maximum current and voltage values for the fruit waste samples were 5.53 V and 11.5 mA,respectively,with a current density of 2.300 mA/cm^(2) and a power density of 12.719 mW/cm^(2).The results indicate the potential for a future development.The next step in development involves determining the optimum conditions for utilizing of rice,vegetable,and fruit waste.The results of the electrical conductivity test on rice,vegetable,and fruit waste samples were 1.51,2.88,and 3.98 mS,respectively,with the highest electrical conductivity value found in the fruit waste sample. 展开更多
关键词 Electrical energy fruit waste rice waste vegetable waste
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A review:Health benefits and physicochemical characteristics of blended vegetable oils
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作者 Hina Daud Memon Sarfaraz Ahmed Mahesar +3 位作者 Sirajuddin Huseyin Kara Syed Tufail Hussain Sherazi Mohammad Younis Talpur 《Grain & Oil Science and Technology》 CAS 2024年第2期113-123,共11页
Oil blending is the method of choice used worldwide to improve oxidative stability and nutritional value.There is no such edible oil/fat that meets all the recommendations from the health point of view.The fatty acid ... Oil blending is the method of choice used worldwide to improve oxidative stability and nutritional value.There is no such edible oil/fat that meets all the recommendations from the health point of view.The fatty acid composition of vegetable oils decides the fate of the oil.Pure single oil is unable to provide a balanced amount of fatty acids(FAs)required/recommended on a daily intake basis.Blending oils/fats is an appropriate procedure of physically mixing multiple oils in suitable proportions which may provide functional lipids with improved antioxidant potential and desirable physical and chemical properties.This review piled up the accessible data on the blending of diverse oils/fats in the combination of binary,ternary,quaternary,or other types of oils into a single blended oil.Blending can be found very convincing towards appropriate FA profile,enhancement in physicochemical characteristics,and augmented stability for the period of storage or when used as cooking/frying processes which could ultimately serve as an effectual dietary intervention towards the health protectiveness. 展开更多
关键词 vegetable oil Oil blending Physicochemical properties Nutritional benefits
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Comprehensive Prevention and Control Technology of Vegetable Diseases and Pests in Shandong Province
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作者 Dongwen SUN Hui YU +7 位作者 Yang QIN Yujie LIN Xiaomeng QI Yuxuan CHEN Yuxin HE Xinying YAN Jia'er XU Shumei GUO 《Plant Diseases and Pests》 2024年第1期26-27,32,共3页
An in-depth research and practice has been conducted on vegetable diseases and pests in Shandong Province,and the principles of comprehensive and ecological control of diseases and pests are put forward,including agri... An in-depth research and practice has been conducted on vegetable diseases and pests in Shandong Province,and the principles of comprehensive and ecological control of diseases and pests are put forward,including agricultural control measures such as crop rotation,field cleaning,fertilizer and water management,physical control measures such as catching and killing,trapping,blocking,photoelectric energy treatment,biological control measures such as the use of natural enemies,pathogenic microorganisms,other beneficial organisms and metabolites,and scientific and rational chemical control measures.Comprehensive prevention and control not only controls vegetable diseases and pests effectively,but also protects the ecological environment. 展开更多
关键词 vegetable Diseases and pests Comprehensive prevention and control
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Drivers,Trends,and Patterns of Changing Vegetation-greenness in Nansha Islands,China from 2016 to 2022
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作者 TANG Jiasheng FU Dongjie +2 位作者 SU Fenzhen YU Hao WANG Xinhui 《Chinese Geographical Science》 SCIE CSCD 2024年第4期662-673,共12页
Changes in vegetation status generally also represents changes in the ecological health of islands and reefs(IRs).However,studies are limited of drivers and trends of vegetation change of Nansha Islands,China and how ... Changes in vegetation status generally also represents changes in the ecological health of islands and reefs(IRs).However,studies are limited of drivers and trends of vegetation change of Nansha Islands,China and how they relate to climate change and human activities.To resolve this limitation,we studied changes to the Normalized Difference Vegetation Index(NDVI)vegetation-greenness index for 22 IRs of Nansha Islands during normal and extreme conditions.Trends of vegetation greenness were analyzed using Sen's slope and Mann-Kendall test at two spatial scales(pixel and island),and driving factor analyses were performed by time-lagged partial correlation analyses.These were related to impacts from human activities and climatic factors under normal(temperature,precipitation,radiation,and Normalized Difference Built-up Index(NDBI))and extreme conditions(wind speed and latitude of IRs)from 2016 to 2022.Results showed:1)among the 22 IRs,NDVI increased/decreased significantly in 15/4 IRs,respectively.Huayang Reef had the highest NDVI change-rate(0.48%/mon),and Zhongye Island had the lowest(–0.29%/mon).Local spatial patterns were in one of two forms:dotted-form,and degradation in banded-form.2)Under normal conditions,human activities(characterized by NDBI)had higher impacts on vegetation-greenness than other factors.3)Under extreme conditions,wind speed(R^(2)=0.2337,P<0.05)and latitude(R^(2)=0.2769,P<0.05)provided limited explanation for changes from typhoon events.Our results provide scientific support for the sustainable development of Nansha Islands and the United Nations‘Ocean Decade’initiative. 展开更多
关键词 island and reefs(IRs) Normalized Difference vegetation Index(NDVI) vegetation-greenness change-rate Sen's slope Nansha Islands China
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Strategies for High-quality Development of Greenhouse Vegetables in Zibo City,Shandong Province
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作者 Dongwen SUN He ZHU +4 位作者 Bo LI Jiaer XU Xinying YAN Yuxin HE Yu SHI 《Asian Agricultural Research》 2023年第12期21-23,共3页
This investigation report got a clear picture of the general situation of the development of greenhouse vegetable industry in Zibo,and found out the existing problems such as frequent harmful weather,few special varie... This investigation report got a clear picture of the general situation of the development of greenhouse vegetable industry in Zibo,and found out the existing problems such as frequent harmful weather,few special varieties and high-grade varieties of greenhouse vegetables,fragmentation of new technology promotion of greenhouse vegetables,low level of intensive seedling raising of vegetables,backward level of facility planting structure and equipment,etc.This paper puts forward the strategies for the future high-quality development of vegetables:promoting the adjustment of vegetable planting structure,rationally arranging vegetables for rotation,strengthening vegetable technical guidance,and innovating vegetable consumption patterns. 展开更多
关键词 greenHOUSE vegetableS High quality STRATEGIES
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Remediation Technologies for Cadmium Contamination in Greenhouse Vegetable Fields
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作者 Huiwei ZHAO Yiming LIU +4 位作者 Wenjun LIU Wei LIU Jing YANG Chuan LU Qiaoying ZHANG 《Meteorological and Environmental Research》 CAS 2023年第2期78-84,共7页
Based on the literature and experimental results, three kinds of soil amendments, namely rice biochar, hydroxyapatite and potassium dihydrogen phosphate(KH2PO4), and deeper ploughing were selected to evaluate the fiel... Based on the literature and experimental results, three kinds of soil amendments, namely rice biochar, hydroxyapatite and potassium dihydrogen phosphate(KH2PO4), and deeper ploughing were selected to evaluate the field application effect of soil amendments and agronomic measures on the remediation of Cd contamination in greenhouse vegetable fields. Cd-contaminated greenhouse screening was conducted from 2015 to 2017. In September 2017, comparative tests of eight treatments were performed, and a preferred test was performed in September 2018. The screening results of the contaminated areas indicated that the distribution of over-standard sites was uneven, and Cd content was significantly different. Over-standard rate of No.4 greenhouse was 83.33% and was the highest, and the average content of Cd in soil was 0.535 mg/kg. It was used as a comparative test greenhouse for eight treatments. No.1 greenhouse was selected as the preferred test greenhouse, with three over-standard plots having average Cd concentrations of 0.530, 0.568 and 0.792 mg/kg. The comparative test results showed that after 8 months of remediation, the content of available Cd in the treatment of hydroxyapatite+rice biochar+deeper ploughing(T6) was reduced by 32.55% compared with CK(the control) and 24.96% than 2 months of remediation. The content of available Cd using the treatment of potassium dihydrogen phosphate+rice biochar+deeper ploughing(T7) decreased by 47.88% compared with CK and 31.00% than 2 months of remediation. The preferred remediation test results showed that in the treatment of hydroxyapatite+rice biochar+deeper ploughing: the mean Cd content decreased from 0.489 to 0.372 mg/kg, reducing by 23.86%, and the mean did not exceed the standard. Compared with CK, the mean content of available Cd decreased by 10.71% after 8 months, and the lowest content of available Cd in three treatments was 0.133 mg/kg. In addition, the Cd content, bioconcentration factor(BCF) and translocation coefficient(TF) of bean aboveground plants were decreased by 15.86%, 23.68% and 25.77%, respectively when compared with CK. Rice biochar+hydroxyapatite +deeper ploughing is a favoured technology for the remediation of Cd-contaminated greenhouse vegetable fields. 展开更多
关键词 greenhouse vegetable fields Cd pollution Remediation technology Application study
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水禽细小病毒SYBR Green Ⅰ荧光定量PCR检测方法的建立与应用
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作者 汪宏才 商雨 +7 位作者 马瑶 曾哲 张蓉蓉 姚伦 罗玲 李丽 温国元 罗青平 《湖北农业科学》 2024年第6期218-222,共5页
为了建立水禽细小病毒(WPV)快速检测方法,根据序列比对结果在水禽细小病毒NS基因SF3保守区域内设计特异性引物,建立SYBR Green Ⅰ荧光定量PCR通用检测方法。该方法的扩增效率(E)为90.0%,相关系数(R~2)=0.99,标准曲线方程为y=-3.607x+38.... 为了建立水禽细小病毒(WPV)快速检测方法,根据序列比对结果在水禽细小病毒NS基因SF3保守区域内设计特异性引物,建立SYBR Green Ⅰ荧光定量PCR通用检测方法。该方法的扩增效率(E)为90.0%,相关系数(R~2)=0.99,标准曲线方程为y=-3.607x+38.77;除WPV出现S形扩增曲线外,新城疫病毒(NDV)、H9亚型禽流感病毒(H9 AIV)、鸭坦布苏病毒(DTMUV)、鸭肝炎病毒(DHAV)、鸭肠炎病毒(DEV)、鸭呼肠孤病毒(DRV)样品均未出现S形阳性扩增曲线;批内变异系数(CV)为0.15%~0.23%,批间变异系数为0.09%~0.28%。结果表明,SYBR Green Ⅰ荧光定量PCR检测方法重复性好、灵敏度高和特异性强。临床样品检测结果表明,SYBR Green Ⅰ荧光定量PCR与普通PCR的符合率达98.4%,灵敏度是普通PCR的1 000倍。SYBR Green Ⅰ荧光定量PCR检测方法不仅能定性检测WPV,还可以进行定量检测,可用于种鸭场、种鹅场的WPV净化检测,也可用于WPV临床大量样品的快速检测。 展开更多
关键词 水禽细小病毒 检测方法 SYBR green 荧光定量PCR
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盆底超声检查和Green分型诊断产妇产后早期盆底结构及压力性尿失禁
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作者 黄雪 周京晶 +3 位作者 李梦妮 白炜 容亚娟 邵越 《中国计划生育学杂志》 2024年第6期1444-1447,共4页
目的:探究盆底超声检查和Green分型在产妇产后早期盆底结构及压力性尿失禁(SUI)诊断中的价值。方法:选取2021年3月-2023年3月本院就诊经产妇204例,根据产后早期(6~8周)SUI发生情况分为SUI组(82例)和非SUI组(122例)。检查产妇静息和Valsa... 目的:探究盆底超声检查和Green分型在产妇产后早期盆底结构及压力性尿失禁(SUI)诊断中的价值。方法:选取2021年3月-2023年3月本院就诊经产妇204例,根据产后早期(6~8周)SUI发生情况分为SUI组(82例)和非SUI组(122例)。检查产妇静息和Valsalva动作状态下膀胱颈距耻骨联合下缘的距离(BSD)、膀胱尿道后角(PUA)、膀胱颈移动度(BND)和尿道旋转角(UR),对比Green分型;采用受试者工作特征(ROC)曲线分析相关参数诊断SUI价值。结果:静息状态下,两组盆底超声参数无差异(P>0.05);SUI组Valsalva动作状态下的BSD、PUA、BND和UR值均大于非SUI组,Green分型Ι型占比(25.6%)低于非SUI组(59.8%)(均P<0.05)。ROC曲线分析,Green分型曲线下面积(AUC)0.579,对SUI诊断价值不佳(P=0.057),Valsalva动作状态下BSD(AUC=0.758)、PUA(AUC=0.743)、BND(AUC=0.769)和UR(AUC=0.705)对SUI诊断价值良好(均P<0.05)。结论:盆底超声检查和Green分型能有效评估经产妇早期盆底结构状况,前者可诊断SUI。 展开更多
关键词 压力性尿失禁 产后早期 盆底结构 超声检查 green分型 诊断
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约束Green函数与非线性地基梁模态分析 被引量:1
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作者 赵翔 王琦 +1 位作者 朱伟东 李映辉 《动力学与控制学报》 2024年第2期77-84,共8页
在生物和医学领域,微机电系统(MEMS)中的微梁结构在植入人体的使用时,由于体内的细胞环境类似于水凝胶,在这种环境下工作,设备和仪器的精度和稳定性很大程度上受到细胞弹性的影响.为了分析此类地基梁的动力学问题,本文建立了非线性基础... 在生物和医学领域,微机电系统(MEMS)中的微梁结构在植入人体的使用时,由于体内的细胞环境类似于水凝胶,在这种环境下工作,设备和仪器的精度和稳定性很大程度上受到细胞弹性的影响.为了分析此类地基梁的动力学问题,本文建立了非线性基础上的梁振动模型,研究了任意位置弹簧和非线性弹簧基础上的梁模态.通过Laplace变换和线性叠加原理,得到了一种约束Green函数,利用数值计算验证方案的有效性,并研究了各种重要物理参数的影响,发现弹簧位置向跨中移动时,模态对称性被打破,弹簧刚度增加,模态阶数改变. 展开更多
关键词 约束green函数 LAPLACE变换 非线性弹簧基础 模态分析 叠加原理
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Vegetable Oil-Based Nanolubricants in Machining:From Physicochemical Properties to Application 被引量:4
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作者 Xiaotian Zhang Changhe Li +9 位作者 Zongming Zhou Bo Liu Yanbin Zhang Min Yang Teng Gao Mingzheng Liu Naiqing Zhang Zafar Said Shubham Sharma Hafiz Muhammad Ali 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2023年第4期4-42,共39页
Cutting fluid is crucial in ensuring surface quality and machining accuracy during machining.However,traditional mineral oil-based cutting fluids no longer meet modern machining’s health and environmental protection ... Cutting fluid is crucial in ensuring surface quality and machining accuracy during machining.However,traditional mineral oil-based cutting fluids no longer meet modern machining’s health and environmental protection require-ments.As a renewable,pollution-free alternative with excellent processing characteristics,vegetable oil has become an inevitable replacement.However,vegetable oil lacks oxidation stability,extreme pressure,and antiwear proper-ties,which are essential for machining requirements.The physicochemical characteristics of vegetable oils and the improved methods’application mechanism are not fully understood.This study aims to investigate the effects of viscosity,surface tension,and molecular structure of vegetable oil on cooling and lubricating properties.The mechanisms of autoxidation and high-temperature oxidation based on the molecular structure of vegetable oil are also discussed.The study further investigates the application mechanism and performance of chemical modification and antioxidant additives.The study shows that the propionic ester of methyl hydroxy-oleate obtained by epoxidation has an initial oxidation temperature of 175℃.The application mechanism and extreme pressure performance of conventional extreme pressure additives and nanoparticle additives were also investigated to solve the problem of insufficient oxidation resistance and extreme pressure performance of nanobiological lubricants.Finally,the study discusses the future prospects of vegetable oil for chemical modification and nanoparticle addition.The study provides theoretical guidance and technical support for the industrial application and scientific research of vegetable oil in the field of lubrication and cooling.It is expected to promote sustainable development in the manufacturing industry. 展开更多
关键词 Cutting fluid vegetable oil Chemical modification ANTIOXIDANT Extreme pressure additive Minimum quality lubrication
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Rapid screening of 420 pesticide residues in fruits and vegetables using ultra high performance liquid chromatography combined with quadrupole-time of flight mass spectrometry 被引量:2
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作者 Junmei Ma Sufang Fan +5 位作者 Liqiong Yang Liangna He Hongwen Zhai Xiaowei Ren Qiang Li Yan Zhang 《Food Science and Human Wellness》 SCIE CSCD 2023年第4期1064-1070,共7页
A new method for screening and identification 420 pesticide residues in fruits and vegetables by ultra-performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry(UPLC-Q-TOF/MS)were deve... A new method for screening and identification 420 pesticide residues in fruits and vegetables by ultra-performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry(UPLC-Q-TOF/MS)were developed.The samples were extracted with acetonitrile/acetic acid(99:1,V/V),and clean-up by SinChERS-Nano(single-step,cheap,effective,rugged,safe,nano)column,determined by UPLC-Q-TOF/MS.The accurate mass database and MS/MS database which contains 420 pesticides were established,the automatic retrieval of detection results was carried on according to the accurate mass,retention time,isotope ratio,ion fragment information,and so on.Method verification was performed on leeks samples.The results showed that 420 pesticides had good linearity in the range of 0.1-100μg/L,and the correlation coeffificients(R2)was greater than 0.990.The limits of detections(LODs)and limits of quantifications(LOQs)of 420 pesticides were in range of 0.05-2.0 and 0.1-5.0μg/L,respectively.The average spike recoveries at 3 levels were 70.1%to 119.7%,and the relative standard deviations(RSD)were lower than 20%(n=6).With this method,a survey of pesticide residues was conducted for 110 samples of 10 different fruits and vegetables,which provided scientific data for ensuring pesticide residue safety of the fruits and vegetables consumed daily by the public.This method was simple,sensitive and accurate,and could be used for rapid screening of 420 pesticide residues in fruits and vegetables. 展开更多
关键词 UPLC-Q-TOF/MS SinChERS-Nano column Pesticide residues Fruits and vegetables
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Selected fermented indigenous vegetables and fruits from Malaysia as potential sources of natural probiotics for improving gut health 被引量:1
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作者 Olaide Olawunmi Ajibola Raymond Thomas Babatunde Femi Bakare 《Food Science and Human Wellness》 SCIE CSCD 2023年第5期1493-1509,共17页
In the Peninsular Malaysia and Northern Borneo island of Malaysia, various rich indigenous leafy vegetables and fruits grow and contribute to the nutritional and dietary values of the population. They have high water ... In the Peninsular Malaysia and Northern Borneo island of Malaysia, various rich indigenous leafy vegetables and fruits grow and contribute to the nutritional and dietary values of the population. They have high water contents, thus, naturally vulnerable to rapid food spoilage. Food preservation and processing play a vital role in the inhibition of food pathogens in fruits and vegetables that are prevalent in Malaysia. Lactic acid fermentation is generally a local-based bioprocess, among the oldest form and well-known for food-processing techniques among indigenous people there. The long shelf life of fermented vegetables and fruits improves their nutritional values and antioxidant potentials. Fermented leaves and vegetables can be utilized as a potential source of probiotics as they are host for several lactic acid bacteria such as Lactobacillus confusus, Weissella paramesenteroides, Enterococcus faecalis, Lactobacillus plantarum, Lactobacillus buchneri, Lactobacillus paracasei, Lactobacillus pentosus, Pediococcus acidilactici, Pediococcus pentosaceus and Leuconostoc mesenteroides. These strains may be more viable in metabolic systems whereby they can contribute to a substantial increase in essential biologically active element than industrial starter cultures. This review is aimed to address some essential fermented fruits and vegetables in Malaysia and their remarkable reputations as a potential sources of natural probiotics. 展开更多
关键词 ETHNIC Leafy vegetables FRUIT FERMENTATION Lactic acid bacteria MALAYSIA
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猪流行性腹泻病毒SYBR Green I实时荧光定量PCR检测方法的建立
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作者 董苏洁 孔宁 +9 位作者 秦文珍 翟焕杰 翟雪滢 杨心雨 童武 郑浩 于海 童光志 李有文 单同领 《中国动物传染病学报》 CAS 北大核心 2024年第2期174-180,共7页
为了建立高效、灵敏的猪流行性腹泻病毒(PEDV)检测方法,本研究从GenBank数据库中获取PEDV N基因序列,扩增出PEDV N基因标准质粒,并在N基因的保守区域内设计了一对特异性荧光定量引物,成功建立了SYBR Green I实时荧光定量PCR检测方法。... 为了建立高效、灵敏的猪流行性腹泻病毒(PEDV)检测方法,本研究从GenBank数据库中获取PEDV N基因序列,扩增出PEDV N基因标准质粒,并在N基因的保守区域内设计了一对特异性荧光定量引物,成功建立了SYBR Green I实时荧光定量PCR检测方法。经过一系列试验表明,该检测方法线性关系良好,R^(2)值为0.99;特异性强,敏感性高,最低可检测至2.23 copies/μL,比普通PCR灵敏约100倍;重复性好,组内变异系数为0.25%~0.43%,组间变异系数为0.67%~0.97%;对于各地区96份临床样品检测出PEDV阳性率为25%。本研究建立的实时荧光定量PCR检测方法为PEDV的临床诊断、流行病学调查以及定量研究提供了有效的检测工具。 展开更多
关键词 猪流行性腹泻病毒 N基因 SYBR green I 荧光定量PCR
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Phytochemical constituents and antibacterial activity of some green leafy vegetables 被引量:2
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作者 Ramesa Shafi Bhat Sooad Al-Daihan 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2014年第3期189-193,共5页
Objective:To investigate the antibacterial activity and photochemicals of five green leafy vegetables against a panel of five bacteria strains.Methods:Disc diffusion method was used to determine the antibacterial acti... Objective:To investigate the antibacterial activity and photochemicals of five green leafy vegetables against a panel of five bacteria strains.Methods:Disc diffusion method was used to determine the antibacterial activity,while kanamycin was used as a reference antibiotic.The phytochemical screening of the extracts was performed using standard methods.Results:All methanol extracts were found active against all the test bacterial strains.Overall maximum extracts shows antibacterial activity which range from 6 to 15 mm.Proteins and carbohydrates was found in all the green leaves,whereas alkaloid.steroids.saponins,flavonoids.tannins were found in most of the test samples.Conclusions:The obtain result suggests that green leafy vegetables have moderate antibacterial activity and contain various pharmacologically active compounds and thus provide the scientific basis for the traditional uses of the studied vegetables in the treatment of bacterial infections. 展开更多
关键词 green LEAFY vegetableS ANTIBACTERIAL ACTIVITY PHYTOCHEMICAL screening.
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Soil microbial characteristics and yield response to partial substitution of chemical fertilizer with organic amendments in greenhouse vegetable production 被引量:15
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作者 RONG Qin-lei LI Ruo-nan +3 位作者 HUANG Shao-wen TANG Ji-wei ZHANG Yan-cai WANG Li-ying 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2018年第6期1432-1444,共13页
Greenhouse vegetable production has been characterized by high agricultural inputs, high temperatures, and high cropping indexes. As an intensive form of agriculture, nutrient cycling induced by microbial activities i... Greenhouse vegetable production has been characterized by high agricultural inputs, high temperatures, and high cropping indexes. As an intensive form of agriculture, nutrient cycling induced by microbial activities in the greenhouses is relatively different from open fields in the same region. However, the responses of soil microbial biomass carbon (MBC) and nitrogen (MBN), enzyme activities, microbial community composition, and yield to organic amendment are not well understood. Therefore, a 5-year greenhouse tomato (Solanum lycopersicum Mill.)-cucumber (Cucumis sativus L.) rotation experiment was conducted. The field experiment comprised 5 treatments: 4/4CN (CN, nitrogen in chemical fertilizer), 3/4CN+1/4MN (MN, nitrogen in pig manure), 2/4CN+2/4MN, 2/4CN+1/4 MN+1/4 SN (SN, nitrogen in corn straw) and 2/4CN+2/4SN. The amounts of nitrogen (N), phosphorus (P2O5), and potassium (K2O) were equal in the five treatments. Starting with the fourth growing season, the optimal yield was obtained from soil treated with straw. MBC, MBN, phospholipid fatty acid (PLFA) profiles, and enzyme activities were significantly changed by 5 years of substitution with organic amendments. Redundancy analysis showed that MBC accounts for 89.5 and 52.3% of the total enzyme activity and total community variability, respectively. The activities of phosphomonoesterase, N-acetyl-glucosaminidase, and urease, and the relative abundances of fungi, actinomycetes, and Gram-negative bacteria were significantly and positively related to vegetable yields. Considering the effects of organic amendments on soil microbial characteristics and vegetable yield, 2/4CN+1/4MN+1/4SN can improve soil quality and maintain sustainable high yield in greenhouse vegetable production. 展开更多
关键词 soil microbial biomass enzyme activity microbial community composition organic amendment greenhouse vegetable
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Role of nitrification inhibitor DMPP(3,4-dimethylpyrazole phosphate) in NO^-_3-N accumulation in greengrocery( Brassica campestris L. ssp. chinensis ) and vegetable soil 被引量:18
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作者 XUChao WULiang-huan +1 位作者 JUXiao-tang ZHANGFu-suo 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2005年第1期81-83,共3页
The influence of nitrification inhibitor(NI) 3,4 dimethylpyrazole phosphate(DMPP) on nitrate accumulation in greengrocery( Brassica campestris L. ssp. chinensis ) and vegetable soil at surface layer were investigated ... The influence of nitrification inhibitor(NI) 3,4 dimethylpyrazole phosphate(DMPP) on nitrate accumulation in greengrocery( Brassica campestris L. ssp. chinensis ) and vegetable soil at surface layer were investigated in field experiments in 2002 and 2003 Results showed that NI DMPP took no significant effect on yields of edible parts of greengrocery, but it could significantly decrease NO - 3 N concentration in greengrocery and in vegetable soil at surface layer. In addition, NI DMPP could reduce the NO - 3 N concentration during the prophase stage of storage. 展开更多
关键词 dimethylpyrazole phosphate(DMPP) NO^-_3-N vegetable soil greengrocery( Brassica campestris L. ssp. chinensis )
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Recent Vegetation Cover Dynamics and Climatic Parameters Evolution Study in the Great Green Wall of Senegal
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作者 Bi Tra Olivier Gore Angora Aman +1 位作者 Yves Kouadio Ody-Marc Duclos 《Journal of Environmental Protection》 CAS 2023年第4期254-284,共31页
The drought recorded in 1970s and 1980s, particularly in the Sahara and Sahel region has greatly affected the population as well as the economies and the eco-systems of this area. In 2007, the African Union launched a... The drought recorded in 1970s and 1980s, particularly in the Sahara and Sahel region has greatly affected the population as well as the economies and the eco-systems of this area. In 2007, the African Union launched a Pan-African program, the Great Green Wall for the Sahara, the Sahel Initiative (GGWSSI) to reverse land degradation and desertification by planting a wall of trees stretching from Dakar to Djibouti. The objective is to improve food security, and support local people to adapt to climate change. This paper aims to evaluate the impacts of the reforestation program in Senegal, fifteen years after it was launched. This study uses a time series of satellite-derived vegetation cover and climatic parameters data to analyze the sustainability of these interventions. Change detection approaches were applied to identify and characterize the drives of the eventual changes. A comparative analysis of reforestation on climatic parameters was explored through the temporal analysis of the vegetation index over the periods 2000-2008 and 2009-2020. An increase in vegetation activity was noted through the NDVI at the interannual (+2% to +8%) and seasonal (+1.5% to 7% for the wet season and 1% to 4% for the dry season) scale and a positive and significant evolution is noted on the trace of the GGW. Also, the period 2009-2020 recorded an increase in rainfall of 2% to 8% of the average value 2000-2020 and 4% to 8% of the rainy season. Soil moisture is the climatic parameter that has increased the most, with an increase of 25% to 54% of the 2000-2020 average, i.e. between 20 mm and 70 mm more. This study shows a significant improvement in the relationship between NDVI and climate parameters after the different reforestation actions of the GGW. 展开更多
关键词 Great green Wall of Senegal vegetation Index PRECIPITATION Soil Moisture
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