Clinical Study on the Effectiveness of Xian Fang Huo Ming Yin for Treating Cutaneous Infections and Promoting Wound Healing in Patients with Perianal Abscess

Objective:To explore the effect of the Xian Fang Huo Ming Yin(XFHM)for treating cutaneous infections and promoting wound healing in patients with perianal abscesses.Methods:Sixty-one patients with perianal abscesses who were admitted to our hospital(Xinghua City People’s Hospital)from May 2022 to May 2023 were selected and randomly divided into two groups,a control group(30 cases)and a study group(31 cases).Both groups received surgical treatment.The control group received conventional treatment and warm water fumigation,sitz bath,and surgical dressing change after surgery,while the research group received XFHM based on the control group.XFHM was taken orally and replaced with warm water for fumigation and sitz bathing.Both groups received treatment for 4 weeks but discontinued sitz bathing after 2 weeks.Various clinical indicators between the two groups were compared.Results:The total clinical effective rate and wound recovery rate of the study group were higher than that of the control group.There were differences in the wound pain scores,surrounding tissue edema,and wound secretions at different time points.Both groups experienced wound pain.The scores of wound pain,surrounding tissue edema,and wound secretions of the study group were lower than those of the control group,7 and 14 days after surgery.The serum interleukin 6(IL-6),tumor necrosis factor-alpha(TNF-α)levels,and pH values of the study group were lower than those of the control group 10 days after surgery(P<0.05).Conclusion:The application of XFHM for treating cutaneous infections and promoting wound healing in patients with perianal abscesses improved the treatment outcome,alleviated clinical symptoms,and promoted healing.

基于BCD工艺的LIN总线收发器设计

针对汽车内部复杂的控制节点与严苛的通讯环境,设计了一款抑制电磁干扰的局域互联网络(Local interconnect network, LIN)总线收发器芯片。基于90 nm BCD高压工艺,采用双向静电放电保护、分段电流驱动、共源级隔离驱动以及总线反馈技术提高系统可靠性。测试结果表明芯片性能符合LIN总线物理层协议规范要求,实现了高低电压域的转换;同时具有良好的抗干扰能力,信号占空比最大变化仅为2.8%,电磁辐射比标准限制值低28 dBμV,达到车用LIN总线通讯相关标准要求。

Mechanism of Huatan Sanjie Fang in improving goiter in Graves'disease mice based on the Hippo signaling pathway

Objective:To explore the mechanism of Huatan Sanjie Fang(HTSJ)in regulating goiter in Graves'disease(GD)mice by detecting key factors of the Hippo signaling pathway.Methods:A mouse model of GD was established by injecting Ad-TSHR289 adenovirus into the bilateral quadriceps femoris of female mice.Successful mouse models were then randomly divided into a model group,methimazole(MMI)group,and HTSJ group,and fed with deionized water,MMI(4.5 mg/kg per day),and HTSJ(35.10 g/kg per day),respectively,for 10 weeks.Histopathological changes of the thyroid gland were subsequently observed by hematoxylin-eosin staining.Radioimmunoassay was used to detect serum total thyroxine(T4)and thyrotrophin-receptor antibody(TRAb)levels.The relative expression of mRNA of Mst1,YAP,and TAZ were detected by quantitative real-time polymerase chain reaction,while the protein expression of Mst1,YAP,TAZ,pMst1,and pYAP were detected by western blot.Results:After 10 weeks of drug intervention,goiter and other pathological changes in the HTSJ group significantly improved compared with the model group,and the levels of serum T4 and TRAb significantly decreased(P=.002,P<.001,respectively).Decreased mRNA expression of Mst1,YAP,and TAZ,the key factors of the Hippo signaling transduction pathway,was also observed(P=.002,P=.022,P<.001,respectively).In contrast,protein expression of Mst1(P=.046),pMst1(P=.026),and p YAP(P=.004)increased,while protein expression of YAP and TAZ decreased(P=.041,P<.001,respectively).Conclusion:HTSJ can effectively improve goiter in GD mice through the Hippo signaling pathway.

LIN28A/B在肿瘤发生发展中的作用研究进展

LIN28A及其同源蛋白LIN28B是高度保守的RNA结合蛋白,在胚胎早期发育、体细胞重编程、葡萄糖代谢以及肿瘤发生发展中具有重要作用。LIN28A/B在乳腺癌等恶性肿瘤中高表达,在肿瘤的起始、维持和转移中均发挥重要作用,并与预后不良相关。研究表明,LIN28A/B主要通过抑制let-7s等microRNA和直接靶向mRNA的方式发挥调控作用。本文主要综述了LIN28A/B在肝癌、乳腺癌、结直肠癌等恶性肿瘤中的功能及其分子调控机制,以期为进一步研究LIN28A/B的作用机制及其在临床治疗中的可能应用提供理论参考。

A Study of the Treatment of Disfiguring Diseases in the “Qian Jin Fang”

Traditional Chinese medicine(TCM)has been passed down for more than 2,000 years.TCM beauty products driven by the principles of identification and treatment with various dosage forms and exhibit mild and safe efficacy are bound to become a main component of the beauty industry in the future.Xu once commented that“Qian Jin Fang”is different from the traditional prescription system,which emphasizes the use of medicines.In this paper,we mainly selected the prescriptions for the treatment of disfigurement diseases such as acne,scarring,black dryness,face hyperpigmentation,black mole,and rosacea from the article titled,“Upper Seven Orifices Disease-Facial Medicine IX”in the Bei Ji Qian Jin Yao Fang[1].By studying and analyzing the original composition of the formula,understanding the etiology and pathogenesis of the disease,and exploring the characteristics of the formula concerning the efficacy of TCM,their properties,flavors,and meridians,we summarized the characteristics of the Qian Jin Fang.It is based on the external treatment of disfigurement diseases,with a wide variety of drugs,flexibility,and accuracy.This method draws upon analogies while utilizing excipients and harmonizers to focus on the combined healing approach of treatment and nourishment.

FPX.BerLIN 狂妄的资本

3月2日,在VCT CN联赛启点赛的第四张地图亚海悬城上,彼时FPX以14比15的比分稍稍落后于EDG,全场的压力给到了FPX的队长兼指挥BerLIN身上,他要完成1打3的残局才能帮队伍拖入加时。然而最终奇迹没能上演,FPX以1比3输给了EDG。

基于Lin-GA的磁性示踪粒子六自由度检测技术

针对基于永磁体和磁传感器阵列的磁定位系统中定位算法存在执行速度慢、定位精度低等问题,提出基于线性算法和遗传算法(linear algorithm-genetic algorithms, Lin-GA)的混合搜索算法。利用自制定位装置中的三轴隧道磁阻(TMR)传感器阵列采集磁性示踪粒子在空间产生的弱磁信号,首先通过线性算法利用磁场绝对向量信息进行粗定位,再利用遗传算法对示踪粒子的六自由度参数进行并行寻优,最终实现对磁性示踪粒子三维位置和三维方向的空间信息反演。实验结果表明,基于Lin-GA的混合搜索算法具有更高的定位精度和更短的执行时间,平均定位误差为(0.55±0.10) mm,平均定向误差为(0.68±0.30)°,平均执行时间为(30.49±4.89) s。

极其震撼的视觉和听觉体验--LINN&Vivid Audio旗舰新品发布会回顾

3月16日(周六)上午,泽森音响在广州东方宾馆8楼东方厅举行了LINN&VividAudio新品发布会,LINN CEO Gilad Tiefenbrun和VIVID Audio总设计师LaurenceDickie亲临现场,为到场媒体和发烧友介绍了此次发布会的两款重磅产品:LINNKlimax Solo800旗舰单声道后级、Vivid Audio全新旗舰音箱MoyaM1。

LINC01980在宫颈癌中的表达及调控LIN28B表达促进宫颈癌生长的作用机制

目的:探讨长链非编码RNA01980(LINC01980)在宫颈癌中的表达及其调控LIN28B促进宫颈癌生长的作用机制。方法:生物信息学工具iBio Tools V5.0(http://www.chrisapp.xyz:3838/R/AnnoE2/)预测宫颈癌差异表达基因,catRAPID数据库(http://service.tartaglialab.com/page/catrapid_group)在线预测相关RNA结合蛋白。选取2020年1月至2021年5月青海红十字医院收治的病理学诊断为宫颈癌的患者60例作为宫颈癌组,选取同期健康体检者(宫颈未发生病变)60例作为健康组,qRTPCR检测两组研究对象血清LINC01980水平;体外培养宫颈癌细胞Hela,转染并分为Lnc-NC组、si-LINC01980组、si-LINC01980+LIN28B-NC组和si-LINC01980+LIN28B组,对照组仅添加新鲜培养液。RNA pull-down和RNA免疫共沉淀试验检测LINC01980与LIN28B相互作用;qRT-PCR检测Hela细胞LINC01980、LIN28B mRNA表达;CCK-8检测细胞活力;流式细胞术检测细胞周期和凋亡;Western blot检测细胞周期蛋白D1(CyclinD1)、周期蛋白依赖性激酶4(CDK4)、半胱氨酸天冬氨酸蛋白酶3(caspase-3)表达。结果:生物信息学工具iBio Tools V5.0预测结果表明,LINC01980在宫颈癌组织中表达上调;与健康组比较,宫颈癌组血清LINC01980 mRNA表达显著升高(P<0.05);生物信息学网站(http://service.tartaglialab.com/page/catrapid_group)预测结果表明,LINC 01980 RNA序列与LIN28存在结合位点,LINC01980与LIN28B存在相互作用;与对照组比较,si-LINC01980组Hela细胞LINC01980、LIN28B mRNA表达、细胞活力、S期、G2/M期细胞百分比、蛋白CyclinD1、CDK4、LIN28B表达显著降低,细胞凋亡率、G0/G1期细胞百分比、蛋白caspase-3表达显著升高(P<0.05);与si-LINC01980组比较,si-LINC01980+LIN28B组Hela细胞活力、S期、G2/M期期细胞百分比、蛋白CyclinD1、CDK4、LIN28B表达显著升高,细胞凋亡率、G0/G1期细胞百分比、caspase-3蛋白表达显著降低(P<0.05)。结论:LINC01980可能通过调控LIN28B影响宫颈癌细胞增殖和凋亡,参与宫颈癌发生发展,可能是宫颈癌治疗的潜在靶点。

RNA结合蛋白Lin28A差异表达可调控牙周膜干细胞的成骨分化

背景:Lin28A能影响细胞活动的多个方面,包括胚胎干细胞的自我更新、体细胞的重编程、个体的生长发育、组织代谢及致癌作用等,而它对牙周膜干细胞成骨分化的影响国内外均没有深入研究,其影响机制更未见报道。目的:探索Lin28A对牙周膜干细胞成骨分化能力的影响。方法:经典酶消化法分离、培养人牙周膜干细胞,qRT-PCR检测Lin28A在该类细胞中的分布情况及其在成骨诱导不同时间的差异性表达;构建Lin28A过表达及干扰表达的慢病毒载体转染至牙周膜干细胞,荧光显微镜观察转染效果;qRT-PCR验证慢病毒转染后Lin28A的表达差异,并进一步从正反两个方面检测成骨诱导培养过程中成骨基因碱性磷酸酶、Runt相关转录因子2和骨桥蛋白的表达量以及碱性磷酸酶染色和茜素红染色,Western blot检测Runt相关转录因子2的蛋白表达水平;应用生物信息学技术预测和双荧光素酶结合实验验证与Lin28A有关的let-7家族成员之间的相关关系,并通过成骨诱导培养差异表达Lin28A的牙周膜干细胞,测定细胞成骨分化过程中表达变化最明显的miRNA。结果与结论:(1)Lin28A富集在牙周膜干细胞的细胞核中,且在成骨诱导不同时间后呈表达上升的趋势;(2)与空白对照组比较,在过表达Lin28A的牙周膜干细胞模型中,碱性磷酸酶染色和茜素红染色相较空白对照组明显深染,成骨基因碱性磷酸酶、Runt相关转录因子2及骨桥蛋白的表达量分别增加3.3倍、5.1倍及2.2倍(P<0.01);干扰Lin28A表达的细胞模型中,碱性磷酸酶染色和茜素红染色相较对照组染色减少,成骨基因碱性磷酸酶、Runt相关转录因子2及骨桥蛋白的表达量分别下降19%,61%及10%(P<0.01);(3)成骨相关转录因子Runt相关转录因子2的蛋白表达水平与mRNA的差异趋势一致,过表达组呈上升趋势,干扰组呈下降趋势;(4)生物信息学预测出与Lin28A相关的let-7家族成员有8个,分别为let-7a、let-7b、let-7c、let-7d、let-7e、let-7f、let-7g和let-7i,经正常培养和成骨诱导培养筛选发现let-7a和let-7c随Lin28A的表达差异而发生明显的变化,以let-7a的表达趋势最显著;双荧光素酶结合实验也证实let-7a与Lin28A的3’-UTR直接结合,参与影响牙周膜干细胞的成骨分化过程;(5)结果提示:Lin28A参与调控牙周膜干细胞成骨分化,呈正性相关,即过表达Lin28A使牙周膜干细胞成骨能力增强,干扰Lin28A表达后牙周膜干细胞成骨能力减弱;牙周膜干细胞成骨分化受到Lin28A的调控影响。

Lin28B和C-myc蛋白在喉鳞癌中的表达及其与临床病理特征和预后的相关性

目的:喉鳞癌是头颈部常见恶性肿瘤。将原癌基因与抑癌基因作为研究的突破口从而筛选恶性肿瘤治疗的靶基因是如今肿瘤研究焦点之一。寻找喉鳞癌治疗及预后相关的靶基因分子至关重要。本研究旨在检测Lin28B和C-myc蛋白在喉鳞癌及其对应癌旁组织中的表达,初步探讨其与喉鳞癌临床病理特征及预后的关系。方法:采用免疫组织化学法检测102例喉鳞癌患者的癌组织及其对应的90例癌旁组织中Lin28B与C-myc蛋白的表达情况,分析Lin28B与C-myc蛋白在喉鳞癌中表达的相关性及其表达水平与喉鳞癌临床病理特征之间的关系。采用Kaplan-Meier法分析喉鳞癌患者术后生存率与Lin28B和C-myc蛋白表达水平之间的关系。结果:与癌旁组织相比,Lin28B、Cmyc蛋白的表达水平在喉鳞癌组织中均显著升高(均P<0.05)。且二者在喉鳞癌中表达存在正相关关系(r=0.476,P<0.05)。Lin28B蛋白的表达与喉鳞癌患者年龄、淋巴结转移情况、临床分期、肿瘤大小和病理分化程度密切相关(均P<0.05)。C-myc蛋白的表达与喉鳞癌患者淋巴结转移情况、临床分期、肿瘤大小和病理分化程度密切相关(均P<0.05)。生存分析显示高表达Lin28B(P=0.001)或C-myc蛋白(P<0.001)的患者术后生存率较低,差异有统计学意义。结论:Lin28B及C-myc蛋白在喉鳞癌组织中高表达且呈正相关,两者均与患者的淋巴结转移情况、临床分期、肿瘤大小、病理分化程度及预后相关,提示Lin28B和C-myc可能都参与喉鳞癌的发生、发展。

RNA结合蛋白LIN28对糖尿病肾病的保护机制研究

目的探索RNA结合蛋白LIN28对糖尿病肾病(DN)的保护机制。方法在HEK 293T细胞中通过腺病毒介导过表达或敲低LIN28。通过RNA-Seq转录组测序和生物信息学分析获得过表达LIN28后显著改变的基因和功能信号通路。体外过表达LIN28实验分组为Control组、Treatment组、Adv-LIN28-OE+D-核糖组和Adv-LIN28-NC+D-核糖组;敲低LIN28实验分组为Control组、Treatment组、Adv-shRNA LIN28+D-核糖组和Adv-shRNA NT+D-核糖组。通过ELISA法测定上述各组细胞上清液人晚期糖基化终产物(AGEs)的水平。通过Western blot法测定上述各组细胞内RAGE、NF-κB和MMP-2的表达水平。结果RNA-Seq转录组测序和GO分析及KEGG分析结果显示,过表达LIN28导致HEK 293T细胞内AGE-RAGE信号通路显著下调(P<0.05)。ELISA结果显示,与Control组相比,Treatment组细胞产生大量AGEs(P<0.05);与Treatment组相比,Adv-LIN28-OE+D-核糖组细胞AGEs水平明显降低(P<0.05),Adv-shRNA LIN28+D-核糖组细胞AGEs水平显著升高(P<0.05)。Western blot结果显示,与Control组相比,Treatment组细胞内RAGE、NF-κB和MMP-2的表达水平显著升高(P<0.05);与Treatment组相比,Adv-LIN28-OE+D-核糖组细胞内RAGE、NF-κB和MMP-2的表达水平显著降低(P<0.05),Adv-shRNA LIN28+D-核糖组细胞内RAGE、NF-κB和MMP-2的表达水平则显著升高(P<0.05)。结论在体外细胞水平上通过腺病毒过表达LIN28,能够导致数千种基因发生显著性差异表达,特别是能够抑制在DN疾病进展中十分关键的糖尿病-并发症相关AGE-RAGE信号通路,发挥保护DN的作用。

基于LIN线通信的迈腾B8L汽车灯光系统原理探析

近年来,随着汽车技术的飞速发展,汽车已呈现出向电动化、智能化、电子化、共享化等多方向发展的趋势,未来的汽车会像手机一样成为人们生活的伙伴,而这一切的发展都离不开汽车数据总线技术的发展与应用。该文主要从大众迈腾B8L汽车灯光系统原理分析与研究出发,阐述了LIN线工作原理及故障原因分析、汽车灯光系统组成及控制原理等,从而为LIN线的故障诊断与维修提供参考。

The role of LIN28B in tumor progression and metastasis in solid tumor entities

LIN28B is an RNA-binding protein that targets a broad range of microRNAs and modulates their maturation and activity.Under normal conditions,LIN28B is exclusively expressed in embryogenic stem cells,blocking differentiation and promoting proliferation.In addition,it can play a role in epithelial-to-mesenchymal transition by repressing the biogenesis of let-7 microRNAs.In malignancies,LIN28B is frequently overexpressed,which is associated with increased tumor aggressiveness and metastatic properties.In this review,we discuss the molecular mechanisms of LIN28B in promoting tumor progression and metastasis in solid tumor entities and its potential use as a clinical therapeutic target and biomarker.

LIN28A attenuates high glucose-induced retinal pigmented epithelium injury through activating SIRT1-dependent autophagy

AIM:To evaluate the effects of LIN28A(human)on high glucose-induced retinal pigmented epithelium(RPE)cell injury and its possible mechanism.METHODS:Diabetic retinopathy model was generated following 48h of exposure to 30 mmol/L high glucose(HG)in ARPE-19 cells.Quantitative real-time polymerase chain reaction(qRT-PCR)and Western blot tested the expression of the corresponding genes and proteins.Cell viability as well as apoptosis was determined through cell counting kit-8(CCK-8)and flow cytometry assays.Immunofluorescence assay was adopted to evaluate autophagy activity.Caspase 3 activity,oxidative stress markers,and cytokines were appraised adopting their commercial kits,respectively.Finally,ARPE-19 cells were preincubated with EX527,a Sirtuin 1(SIRT1)inhibitor,prior to HG stimulation to validate the regulatory mechanism.RESULTS:LIN28A was downregulated in HG-challenged ARPE-19 cells.LIN28A overexpression greatly inhibited HGinduced ARPE-19 cell viability loss,apoptosis,oxidative damage as well as inflammatory response.Meanwhile,the repressed autophagy and SIRT1 in ARPE-19 cells challenged with HG were elevated after LIN28A overexpression.In addition,treatment of EX527 greatly inhibited the activated autophagy following LIN28A overexpression and partly abolished the protective role of LIN28A against HG-elicited apoptosis,oxidative damage as well as inflammation in ARPE-19 cells.CONCLUSION:LIN28A exerts a protective role against HG-elicited RPE oxidative damage,inflammation,as well as apoptosis via regulating SIRT1/autophagy.

EFFECTS OF JIANG TANG FANG LONG FORMULA ON INSULIN PRODUCTION AND FUNCTION IN AN ANIMAL MODEL OF DIABETIC HEARING LOSS

Objective To study the influence of Jiang Tang Fang Long formula on insulin production and function in an animal model of diabetic hearing loss. Methods Wistar rats (n=60) were randomly divided into 6 groups (10 in each) to receive no treatment (the normal control, Group A), or to receive intra-peritoneal 55 mg/kg streptozotocin with (Groups C, D and E) or without (Group B) subsequent Jiang Tang Fang Long formula treatment at various doses or Yu Long Wan treatment (Group F). After 60 days, fasting blood glucose (FBG), body weight (BW) and fasting insulin (FINS) were recorded and the HOMA-IR and HOMA-βcalcu-lated. Insulin expression in pancreatic tissues was measured by radioimmunoassay. Results Compared with animals that received streptozotocin without rescue treatment (Group B), animals that received higher doses of Jiang Tang Fang Long formula(Groups D and E) showed improved indices of diabetes manifestation (P<0.05) and improved HOMA-β(P<0.05) in a dose-dependent manner, as well as improved insulin expression in pancreatic islets (P<0.05). The difference between low dose Jiang Tang Fang Long formula treatment (Group C) and Group B was not significant (P>0.05). Conclusion Our results suggest that Jiang Tang Fang Long formula may improve pancreatic β-cells function which may explain its efficacy in treating diabetic hearing loss.

LIN总线技术在汽车发电机电压调节系统中的应用

发电机是发动机的动力来源。为了确保发动机提供稳定的动力性能,避免因动力不足而导致车辆抖动、影响驾驶舒适性甚至导致发动机无法正常工作,发电机必须实时且精确地调节输出电压。因此,发电机对电压调节速率、输出信号稳定性和故障诊断能力有着极高的要求。本文详细阐述了一种串行通讯网络(LIN总线)技术的通信原理和协议规范,介绍了LIN总线技术在发电机电压调节系统中的应用。并对其实时控制策略进行了分析,说明了控制帧和反馈帧传输的多参数设置规则和诊断功能。最后,通过仿真设备(Emulin)采集了发电机LIN总线通信数据,并借助上位机软件(Visulin)对实际故障问题进行了分析解决。本研究为发电机应用中的LIN总线故障诊断分析提供了参考思路。

The effect of Anshen Dingzhi Fang on tau protein phosphorylation and DNF/TrkB signaling pathway in Alzheimer's disease rats

Objective:To observe the effect of Anshen Dingzhi Decoction on tau phosphorylation in hippocampus of Alzheimer's disease rat model and its related mechanism.Methods:SD rats were randomly divided into control group,model group,Anshen Dingzhi Decoction(low,medium and high dose group)and Dopazide group.Except for the control group,the rats in the other groups were injected with streptozotocin into the lateral ventricle to replicate the model of Alzheimer's disease and then given corresponding drugs for 2 weeks.orris water maze test was used to detect learning and memory ability of rats,HE staining was used to detect hippocampal histological morphology,Western-blot was used to detect tau phosphorylation level and expression abundance of BDNF and TrkB protein in hippocampal tissue of rats.Results:The escape latency of the model group was significantly increased,the number of crossing platforms and effective residence time were significantly reduced,the phosphorylation level of tau protein was significantly increased,and the phosphorylation levels of BDNF and TrkB protein were significantly decreased when compared with the control group,the difference has statistically significant(P<0.05).Anshen Dingzhi Fang could significantly reduce the escape latency of AD rats,increase the number of crossing platforms and effective residence time,inhibit the phosphorylation of tau protein,and up-regulate the phosphorylation of BDNF and TrkB protein,the difference was statistically significant when compared with the model group(P<0.05).Conclusion:Anshen Dingzhi Fang can inhibit the phosphorylation of tau protein by activating BDNF/TrkB signaling pathway and promote the learning and memory ability of AD rats.

Possible mechanisms associated with immune escape and apoptosis on anti-hepatocellular carcinoma effect of Mu Ji Fang granules

BACKGROUND Hepatocellular carcinoma(HCC)is one of the most common digestive system cancers with high mortality rates worldwide.The main ingredients in Mu Ji Fang Granules(MJF)are alkaloids,flavonoids,and polysaccharides.MJF has been used in the clinical treatment of hepatitis,cirrhosis and HCC for more than 30 years.Few previous studies have focused on the mechanism of MJF on tumor immunology in the treatment of HCC.AIM To explore the mechanism of action of MJF on tumor immunology in the treatment of HCC.METHODS The absorbable ingredients of MJF were identified using Molecule Network related to High Performance Liquid Chromatography-Electron Spray Ionization-Time of Flight-Mass Spectrometry,and hub potential anti-HCC targets were screened using network pharmacology and pathway enrichment analysis.Forty male mice were randomly divided into the Blank,Model,and MJF groups(1.8,5.4,and 10.8 g/kg/d)following 7 d of oral administration.Average body weight gain,spleen and thymus indices were calculated,tumor tissues were stained with hematoxylin and eosin,and Interferon gamma(IFN-γ),Tumor necrosis factorα(TNF-α),Interleukin-2,aspartate aminotransferase,alanine aminotransferase,alpha-fetoprotein(AFP),Fas,and FasL were measured by Enzyme-linked Immunosorbent Assay.Relevant mRNA expression of Bax and Bcl2 was evaluated by Real Time Quantitative PCR(RTqPCR)and protein expression of Transforming growth factorβ1(TGF-β1)and Mothers against decapentaplegic homolog(SMAD)4 was assessed by Western blotting.The HepG2 cell line was treated with 10 mg/mL,20 mg/mL,30 mg/mL,40 mg/mL of MJF,and another 3 groups were treated with TGF-β1 inhibitor(LY364947)and different doses of MJF.Relevant mRNA expression of TNF-α,IFN-γ,Bax and Bcl2 was evaluated by RT-qPCR and protein expression of TGF-β1,SMAD2,p-SMAD2,SMAD4,and SMAD7 was assessed by Western blotting.RESULTS It was shown that MJF improved body weight gain and tumor inhibition rate in H22 tumorbearing mice,protected immune organs and liver function,reduced the HCC indicator AFP,affected immunity and apoptosis,and up-regulated the TGF-β1/SMAD signaling pathway,by increasing the relative expression of TGF-β1,SMAD2,p-SMAD2 and SMAD4 and decreasing SMAD7,reducing immune factors TNF-αand IFN-γ,decreasing apoptosis cytokines Fas,FasL and Bcl2/Bax,and inhibiting the effect of LY364947 in HepG2 cells.CONCLUSION MJF inhibits HCC by activating the TGF-β1/SMAD signaling pathway,and affecting immune and apoptotic cytokines,which may be due to MJF adjusting immune escape and apoptosis.

针刺腧穴“降压方”对自发性高血压大鼠ACE-AngⅡ-AT1R轴及Profilin-1的影响

目的研究针刺腧穴“降压方”对自发性高血压大鼠(SHR)的降压效应及对ACE-AngⅡ-AT1R轴、Profilin-1的影响,探讨针刺降压的作用机制。方法按随机数字表法将30只SHR均分为模型组、针刺组,另以15只Wistar Kyoto Rats(WKR)作为正常对照组。针刺组取双侧人迎穴、曲池穴、足三里穴进行针刺,模型组随意在大鼠躯干部位进行针刺,对照组不针刺,其他操作相同。每周连续干预6 d休息1 d,连续4周,共24次。采用大鼠无创血压测量系统测量血压,并记录一般行为变化,采用ELISA法检测大鼠血清中血管紧张素转化酶(ACE)、血管紧张素Ⅱ(AngⅡ)含量,采用免疫组化法(IHC)检测主动脉中血管紧张素Ⅱ受体1(AT1R)、前纤维蛋白-1(Profilin-1)蛋白表达的水平。结果针刺组第2周开始收缩压、舒张压持续降低,模型组收缩压、舒张压逐渐升高,对照组血压未见明显变化;针刺组较模型组一般行为得到改善。实验4周后针刺组血清中ACE、AngⅡ较模型组显著降低,AT1R、Profilin-1表达水平降低。结论针刺腧穴“降压方”对SHR降压效应明确,其机制可能是通过降低SHR血清中ACE、AngⅡ含量,影响SHR主动脉组织中的AT1R和Profilin-1表达,进而抑制局部肾素-血管紧张素-醛固酮系统(RAAS)的活性起到降低血压的作用。