Through analyzing the present landform and geological conditions of Han Yangling Mausoleum, investigating the features of gardening and mausoleum construction in Han Dynasty, this study elaborated expression forms of ...Through analyzing the present landform and geological conditions of Han Yangling Mausoleum, investigating the features of gardening and mausoleum construction in Han Dynasty, this study elaborated expression forms of each landscape element in the ecological restoration of Han Yangling Mausoleum.展开更多
GPX-GI is a cytosolic tetrameric Se-dependent glutathione peroxidase, similar in properties to GPX-1. Unlike the almost ubiquitous GPX-1, GPX-GI is mainly expressed in the epithelium of gastrointestinal tract. GPX-GI ...GPX-GI is a cytosolic tetrameric Se-dependent glutathione peroxidase, similar in properties to GPX-1. Unlike the almost ubiquitous GPX-1, GPX-GI is mainly expressed in the epithelium of gastrointestinal tract. GPX-GI contributes to at least fifty percent of GPX activity in rodent small intestmal epithelium. The total GPX activity consists of at least 70% of selenium-dependent GPX activity in this compartment.By analyzing a panel of mouse mterspecies DNA from the Jackson Laboratory's backcross resource,we mapped Gpx2 gene to mouse chromosome 12 between D12Mit4 and D12Mit5, near the Ccs1 locus which contains a colon cancer susceptibility gene. A pseudogene, Gpx2-ps is mapped to mouse chromosome 7.Comparison of Gpx2 gene expression in three pairs of C57BL/6Ha and ICR/Ha mice which are respectively resistant and sensitive to dimethylhydrazine-induced colon cancer, we found a higher Gpx2 mRNA level in C57BL/6Ha colon than ICR/Ha colon. Interestingly, a lower level of GPX activity is found in the resistant strain of mice. Because GPX-1 has three times higher specific activity than GPX GI, our data suggest that the decreased GPX activity may result from a higher level of Gpx2 gene expression in those cells co-express GPx1 gene展开更多
The image expression of traditional villages is studied through the analysis of their forms.With the famous historical and cultural villages in southern Hebei as the research objects,the relationship between the form ...The image expression of traditional villages is studied through the analysis of their forms.With the famous historical and cultural villages in southern Hebei as the research objects,the relationship between the form and image of traditional villages is deeply analyzed through the survey of village layout and courtyard layout,which can deeply explain the characteristics of regional architectural culture.展开更多
BACKGROUND: Although the hepatoma-specific band of gamma-glutamyltransferase ( GGT ) is a highly sensitive marker in diagnosis of hepatocellular carcinoma, the kine- tic expression and the early alterations of GGT in ...BACKGROUND: Although the hepatoma-specific band of gamma-glutamyltransferase ( GGT ) is a highly sensitive marker in diagnosis of hepatocellular carcinoma, the kine- tic expression and the early alterations of GGT in the deve- lopment of hepatoma remain unclear. In this study, we in- vestigated the expression and the alterations of GGT multi- ple molecular forms in hepatotumorigenesis. METHODS: The expression of GGT in a chemically in- duced hepatocarcinogenesis model was examined by giving 0. 05% of 2-fluoenylacetamide in diet for 12 weeks. The ex- pression levels of total RNA and GGT, and the changes of liver pathology, GGT multiple molecular forms and sugar- chain heterogeneity were investigated at the different stages of rat hepatoma development. RESULTS: Pathological examination and biochemical ana- lysis found that liver GGT was over-expressed and secreted into blood during canceration. Serum total GGT and liver GGT specific activities (IU/g) including soluble and mem- brane-combined GGT were significantly higher (P <0.05) in experimental groups than those in control group, respec- tively. A highly positive correlation was found between to- tal GGT activities and total RNA levels (r =0.90, P <0.05) of the liver. Both were higher six weeks later than before. Con A-non-reactive-GGT was increased consistantly dur- ing the development of rat hepatoma. GGT multiple mo- lecular forms in the liver and sera of experimental rats showed that fetal liver-type GGT bands were associated with the development of hepatoma. CONCLUSIONS: Fetal liver-type GGT in sera and the liver of rats is closely related to hepatotumorigenesis. It can be used as a sensitive enzymatic marker for the early diagnosis of liver cancer.展开更多
Objective： To achieve the co-expression of GrB and PFP in Hep-2 cells and analyze the growth inhibiting effects on Hep-2 cells. Methods： Lymphocytes were separated from human laryngeal carcinoma tissue, complete Exo...Objective： To achieve the co-expression of GrB and PFP in Hep-2 cells and analyze the growth inhibiting effects on Hep-2 cells. Methods： Lymphocytes were separated from human laryngeal carcinoma tissue, complete Exon fragments of GrB and PFP were amplified by RT-PCR via extracting lymphocytes total RNA, and they were recombined to the downstream of T7 promoter in the vector pVAX1. The recombinant plasmid pVAX1-PIG was transfected into Hep-2 cells with Lipofectamine 2000. The expression of proteins was identified by RT-PCR, MTT and western blot assay. Results： The gene sequence of the RT-PCR products of GrB and PFP were consistent with the data of GenBank by DNA sequencing analysis. The GrB and PFP cDNA fragment were cloned into the vector of pVAX1 in the right direction and the open reading fragment of GrB and PFP were maintained. The target proteins were detected in the transfected Hep-2 cells, and the inhibitive effect of PFP and GrB on Hep-2 cells growth were studied by thiazolyl blue （MTT） test. Conclusion： The pVAX1-PFP-IRES-GrB plasmid was successfully constructed and expressed, and the expression of PFP and GrB could inhibit the growth of Hep-2 cells.展开更多
Black female writer, Alice Walker is always misunderstood as a writer who attaches more attention to the contents and thoughts in her works. But the success of her work The Color Purple fully demonstrates her ability ...Black female writer, Alice Walker is always misunderstood as a writer who attaches more attention to the contents and thoughts in her works. But the success of her work The Color Purple fully demonstrates her ability of exquisitely adopting artistic expressions to realize the integration of contents and form. So this paper aims to study three artistic expressions applied in this novel, that is, the epistolary style, symbolism of the real objects and psychological transformation of the characters. Furthermore, it's illustrational to show in this thesis that Alice Walker is not only a writer with deep thoughts, but also an imaginative and creative master of excelling in her writing techniques.展开更多
A numerical approach is an effective means of solving boundary value problems(BVPs).This study focuses on physical problems with general partial differential equations(PDEs).It investigates the solution approach throu...A numerical approach is an effective means of solving boundary value problems(BVPs).This study focuses on physical problems with general partial differential equations(PDEs).It investigates the solution approach through the standard forms of the PDE module in COMSOL.Two typical mechanics problems are exemplified:The deflection of a thin plate,which can be addressed with the dedicated finite element module,and the stress of a pure bending beamthat cannot be tackled.The procedure for the two problems regarding the three standard forms required by the PDE module is detailed.The results were in good agreement with the literature,indicating that the PDE module provides a promising means to solve complex PDEs,especially for those a dedicated finite element module has yet to be developed.展开更多
文摘Through analyzing the present landform and geological conditions of Han Yangling Mausoleum, investigating the features of gardening and mausoleum construction in Han Dynasty, this study elaborated expression forms of each landscape element in the ecological restoration of Han Yangling Mausoleum.
文摘GPX-GI is a cytosolic tetrameric Se-dependent glutathione peroxidase, similar in properties to GPX-1. Unlike the almost ubiquitous GPX-1, GPX-GI is mainly expressed in the epithelium of gastrointestinal tract. GPX-GI contributes to at least fifty percent of GPX activity in rodent small intestmal epithelium. The total GPX activity consists of at least 70% of selenium-dependent GPX activity in this compartment.By analyzing a panel of mouse mterspecies DNA from the Jackson Laboratory's backcross resource,we mapped Gpx2 gene to mouse chromosome 12 between D12Mit4 and D12Mit5, near the Ccs1 locus which contains a colon cancer susceptibility gene. A pseudogene, Gpx2-ps is mapped to mouse chromosome 7.Comparison of Gpx2 gene expression in three pairs of C57BL/6Ha and ICR/Ha mice which are respectively resistant and sensitive to dimethylhydrazine-induced colon cancer, we found a higher Gpx2 mRNA level in C57BL/6Ha colon than ICR/Ha colon. Interestingly, a lower level of GPX activity is found in the resistant strain of mice. Because GPX-1 has three times higher specific activity than GPX GI, our data suggest that the decreased GPX activity may result from a higher level of Gpx2 gene expression in those cells co-express GPx1 gene
文摘The image expression of traditional villages is studied through the analysis of their forms.With the famous historical and cultural villages in southern Hebei as the research objects,the relationship between the form and image of traditional villages is deeply analyzed through the survey of village layout and courtyard layout,which can deeply explain the characteristics of regional architectural culture.
基金This study was supported by a grant from Key Projects of Medical Sciences Department of Health , Jiangsu Province, China ( RC2003100 ).
文摘BACKGROUND: Although the hepatoma-specific band of gamma-glutamyltransferase ( GGT ) is a highly sensitive marker in diagnosis of hepatocellular carcinoma, the kine- tic expression and the early alterations of GGT in the deve- lopment of hepatoma remain unclear. In this study, we in- vestigated the expression and the alterations of GGT multi- ple molecular forms in hepatotumorigenesis. METHODS: The expression of GGT in a chemically in- duced hepatocarcinogenesis model was examined by giving 0. 05% of 2-fluoenylacetamide in diet for 12 weeks. The ex- pression levels of total RNA and GGT, and the changes of liver pathology, GGT multiple molecular forms and sugar- chain heterogeneity were investigated at the different stages of rat hepatoma development. RESULTS: Pathological examination and biochemical ana- lysis found that liver GGT was over-expressed and secreted into blood during canceration. Serum total GGT and liver GGT specific activities (IU/g) including soluble and mem- brane-combined GGT were significantly higher (P <0.05) in experimental groups than those in control group, respec- tively. A highly positive correlation was found between to- tal GGT activities and total RNA levels (r =0.90, P <0.05) of the liver. Both were higher six weeks later than before. Con A-non-reactive-GGT was increased consistantly dur- ing the development of rat hepatoma. GGT multiple mo- lecular forms in the liver and sera of experimental rats showed that fetal liver-type GGT bands were associated with the development of hepatoma. CONCLUSIONS: Fetal liver-type GGT in sera and the liver of rats is closely related to hepatotumorigenesis. It can be used as a sensitive enzymatic marker for the early diagnosis of liver cancer.
基金the Foundation of Doctoral Subject in Jinan University(51205069)the Natural Science Foundation of Guangdong,China(5300804)
文摘Objective： To achieve the co-expression of GrB and PFP in Hep-2 cells and analyze the growth inhibiting effects on Hep-2 cells. Methods： Lymphocytes were separated from human laryngeal carcinoma tissue, complete Exon fragments of GrB and PFP were amplified by RT-PCR via extracting lymphocytes total RNA, and they were recombined to the downstream of T7 promoter in the vector pVAX1. The recombinant plasmid pVAX1-PIG was transfected into Hep-2 cells with Lipofectamine 2000. The expression of proteins was identified by RT-PCR, MTT and western blot assay. Results： The gene sequence of the RT-PCR products of GrB and PFP were consistent with the data of GenBank by DNA sequencing analysis. The GrB and PFP cDNA fragment were cloned into the vector of pVAX1 in the right direction and the open reading fragment of GrB and PFP were maintained. The target proteins were detected in the transfected Hep-2 cells, and the inhibitive effect of PFP and GrB on Hep-2 cells growth were studied by thiazolyl blue （MTT） test. Conclusion： The pVAX1-PFP-IRES-GrB plasmid was successfully constructed and expressed, and the expression of PFP and GrB could inhibit the growth of Hep-2 cells.
文摘Black female writer, Alice Walker is always misunderstood as a writer who attaches more attention to the contents and thoughts in her works. But the success of her work The Color Purple fully demonstrates her ability of exquisitely adopting artistic expressions to realize the integration of contents and form. So this paper aims to study three artistic expressions applied in this novel, that is, the epistolary style, symbolism of the real objects and psychological transformation of the characters. Furthermore, it's illustrational to show in this thesis that Alice Walker is not only a writer with deep thoughts, but also an imaginative and creative master of excelling in her writing techniques.
基金supported by the National Natural Science Foundations of China(Grant Nos.12372073 and U20B2013)the Natural Science Basic Research Program of Shaanxi(Program No.2023-JC-QN-0030).
文摘A numerical approach is an effective means of solving boundary value problems(BVPs).This study focuses on physical problems with general partial differential equations(PDEs).It investigates the solution approach through the standard forms of the PDE module in COMSOL.Two typical mechanics problems are exemplified:The deflection of a thin plate,which can be addressed with the dedicated finite element module,and the stress of a pure bending beamthat cannot be tackled.The procedure for the two problems regarding the three standard forms required by the PDE module is detailed.The results were in good agreement with the literature,indicating that the PDE module provides a promising means to solve complex PDEs,especially for those a dedicated finite element module has yet to be developed.
