低温诱导甜菜(Beta vulgaris L.)Ty7Br600基因的Northern blotting和Southern blotting分析

以低温诱导甜菜幼苗为试验材料,以未进行低温诱导的甜菜幼苗为对照,采用α-32P-ATP放射标记按随机引物标记法标记本实验室克隆的Ty7Br600基因,进行Northern杂交。Northern杂交试验结果显示,在低温诱导培养的甜菜幼苗的RNA群体中,出现较强的杂交条带,而在未诱导培养的甜菜幼苗的RNA群体中,则几乎没有阳性杂交条带出现。因此,Ty7Br600这一序列有可能是二年生甜菜幼苗经低温诱导处理之后被诱导表达的与抽薹相关的新基因序列。Southern杂交结果发现,在每一组酶切中至少有2~3条条带,表明Ty7Br600确为甜菜基因组片段,也同时表明该基因在甜菜基因组中以2个拷贝或低拷贝形式存在。

The Southern Oscillation / Northern Oscillation Cycle Associated with Sea Surface Temperature in the Equatorial Pacific

This paper analyzed the time evolution of the global 1000 hPa height anomalies related to the sea surface temperature (SST) in the eastern equatorial Pacific by using ECMWF data in the period 1979-1988, in which two Pacific warm events, 1982/83 and 1986/787, are included. It is found that there are distinct evidences of eastward propagation of alternate positive / negative height anomalies not only in the tropical South Pacific but also in the tropical North Pacific. The former is associated with the Southern Oscillation (SO) and the latter is associated with the so-called Northern Oscillation (NO).It is noteworthy that the alternate positive / negative anomaly centers associated with SO and NO can be traced back to the middle and higher latitudes of the South Indian Ocean and the East Asian continent respectively, which may be significant for the understanding of the causes and mechanism of SO and NO and for the monitoring of ENSO.Furthermore, these evolution processes have a strong symmetry about the Northern and the Southern Hemisphere. The whole SO/ NO cycle takes about 3.5 years from extreme positive to negative index pattern and again from extreme negative to positive index pattern. It is suggested that the anomalous cold air activities over East Asia and Australia and the polar vortex activities over the northern and the southern Pacific seem to have dominant role on the SO / NO cycle.

Differential Hydrocarbon Accumulation Controlled by Structural Styles along the Southern and Northern Tianshan Thrust Belt

The Kuqa and the Southern Junggar foreland thrust belts, which lie to the southern and northern Tianshan, respectively, were formed under a strong compressional tectonic setting. Due to the differential propagation and deformation under the control of the décollement horizon, the structural deformation styles differ in the Kuqa and Southern Junggar thrust belts. Imbricated stacking is developed in the Kuqa thrust belt, forming a piggyback imbricated pattern of faulted anticline and fault-block structural assemblage dominated by salt structures. In contrast, wedge-shaped thrusts are developed in Southern Junggar, mainly forming vertical laminated patterns of multi-wedge-structure stacks strongly influenced by the décollement horizons. The different deformation patterns and structural styles of the north and south of Tian Shan control the contrasting characteristics of hydrocarbon accumulation in the foreland thrust belts of the Kuqa and the Southern Junggar thrust belts, including the variance in the hydrocarbon trap types, pathway systems and hydrocarbon-bearing horizons. Proven by the hydrocarbon accumulation research and exploration achievements, recent exploration targets should focus on sub-salt piggyback imbricated structural patterns in the Kuqa and the deep laminated patterns in the Southern Junggar thrust belt.

An Outline of Mesozoic to Paleogene SequenceStratigraphy and Sea－Level Changes in Northern Himalayas，Southern Xizang

In the Northern Himalayan region of Southern Xizang (Tibet),from the Lower Triassic to Upper Eocene 73 Sequences have been identified, with an average duration of 2.9 Ma;these can in turn be grouped into 24 super-Sequences and 6 super-sequence sets. During Mesozoic and Paleogene, several large sea - level falls occurred in the Eastern Neo-Tethys. Among the recognized sea- level falls, the important ones include those at the ages of 255 Ma, 215 Ma,177 Ma, 138 Ma, 103 Ma and 68 Ma .Those at 239 Ma, 215 Ma, 157 Ma,80 Ma, 50Ma and 36 Ma are also significant. The third-order Sequences and sea-level cycles Probably reflect mainly global sea - level fluctuations, while the higher rank cycles seem more closely related to the basin evolution of the Neo-Tethys. Based on the study, six major periods have been suggested for ths tectonic evolution of the Eastern Neo-Tethys and the plates, i. e. the Pangea Period (Pre-Triassic), continental rifting Period (Triassic to Early Jurassic ), inter-continental sea Period (Middle Jurassic ), continental divergence period (late Middle Jurassic to Early Cretaceous ), continental convergence period (Late Cretaceous ) and the continental collision Period (Paleogene ). These major Periods can be further subdivided into eight stages according to the basin evolution. In each of the periods and Stages, Sequences and their boundaries show clear characters related to the tectonic background. The study indicater that the initial breakup of the Pangea along the Indus- Yarlung may have taken Place around 239 Ma. The Late Bathonian to Early Callovian seems to have been a critical time in the evolution of the Neo-Tethys, with the turning Point around 158 Ma. The blocks split from the northern margin of the Gondwana continual did not obviously drift away from the Indian Plate until Callovian .The oceanic crust subduction in the Neo- Tethys may have Started at 113Ma, while the contraction of the ocean probably began at 107- 103 Ma. The initial contact of the Indian Plate with the Eurasian plate may have taken Place around 80 Ma, with strong uplifting and thrushng in Late Paleocene.

The circulation in the southern Huanghai Sea and northern East China Sea in June 1999

On the basis of hydrographic data and current measurement (the mooring system, vessel-mounted ADCP and toward ADCP) data obtained in June 1999, the circulations in the southern Huanghai Sea (HS) and northern East China Sea (ECS) are computed by using the modified inverse method. The Kuroshio flows northeastward through eastern part of the investigated region and has the main core at Section PN, a northward flow at the easternmost part of Section PN, a weaker anti-cyclonic eddy between these two northward flows, and a weak cyclonic eddy at the western part of Section PN. The above current structure is one type of the current structures at Section PN in ECS. The net northward volume transport (VT) of the Kuroshio and the offshore branch of Taiwan Warm Current (TWCOB) through Section PN is about 26.2 x 10(6) m(3)/s in June 1999. The VT of the inshore branch of Taiwan Warm Current (TWCIB) through the investigated region is about 0.4 x 10(6) m(3)/s. The Taiwan Warm Current (TWC) has much effect on the currents over the continental shelf. The Huanghai Sea Coastal Current (HSCC) flows southeastward and enters into the northwestern part of investigated region, and flows to turn cyclonically, and then it flows northeastward, due to the influences of the Taiwan Warm Current and topography. There is a cyclonic eddy south of Cheju Island where the Huanghai Sea Coastal Current flows to turn cyclonically. It has the feature of high dense and cold water. The uniform and cold water is occurred in the layer from about 30 m level to the bottom between Stations C306 and C311 at the northernmost Section C3. It is a southern part of the Huanghai Sea Cold Water Mass (HSCWM).

Influence of climatic warming in the Southern and Northern Hemi-sphere on the tropical cyclone over the western North Pacific Ocean

Based on analyzing the surface air temperature series in the Southern and Northern Hemisphere and the tropical cyclone (TC) over the western North Pacific Ocean, the relationships between climatic warming and the frequency and intensity of tropical cyclone are investigated. The results showed that with the climatic warming in both hemispheres, the frequency of the tropical cyclone over the western North Pacific Ocean reduces and its intensity weakens simultaneously. A possible explanation might be that the cold air invasion from the Southern Hemisphere weakens due to global warming.

Comparisons of surface Chl a and primary productivity along three transects of the southern South China Sea, northern Java Sea and eastern Indian Ocean in April 2011

Results are presented about the changes in chlorophyll a density, carbon fixation and nutrient levels in the surfacewaters of three transects of the southern South China Sea （SCS）, northern Java Sea （JS） and eastern Indian Ocean （IO） duringApril 5-16 of 2011. The in situ Chl a concentration and carbon fixation showed decreasing trends from high to low latitudealong the three transects, while the photosynthetic rate of phytoplankton estimated from 14C incorporation displayed no simplevariation with latitude. Chl a concentration and carbon fixation in the IO water was lower than that in the JS water. Highersalinity and lower contents of dissolved inorganic nitrogen （DIN） and silicate （SiO3^2-） characterized the IO water as comparedto the SCS or JS water, and the PO4^3- content was lower in the IO water than in the SCS or JS water in most cases. Our resultsalso indicate the importance of DIN and SiO3^2- concentrations for the geographical changes in phytoplankton biomass andprimary productivity among the three regions.

A PRINCIPAL MODE OF CIRCULATION COVARIATION BETWEEN THE NORTHERN AND SOUTHERN HEMISPHERES AND ITS ASSOCIATION WITH ENSO DURING BOREAL WINTER

By employing the singular value decomposition(SVD) analysis, we have investigated in the present paper the covariations between circulation changes in the Northern(NH) and Southern Hemispheres(SH) and their associations with ENSO by using the NCEP/NCAR reanalysis, the reconstructed monthly NOAA SST, and CMAP precipitation along with NOAA Climate Prediction Center(CPC) ENSO indices. A bi-hemispheric covariation mode(hereafter BHCM) is explored, which is well represented by the first mode of the SVD analysis of sea surface pressure anomaly(SLPA-SVD1). This SVD mode can explain 57.36% of the total covariance of SLPA. BHCM varies in time with a long-term trend and periodicities of 3—5 years. The long term trend revealed by SVD1 shows that the SLP increases in the equatorial central and eastern Pacific but decreases in the western Pacific and tropical Indian Ocean, which facilitates easterlies in the lower troposphere to be intensified and El Ni觡o events to occur with lower frequency. The spatial pattern of the BHCM looks roughly symmetric about the equator in the tropics, whereas it is characterized by zonal disturbances in the mid-latitude of NH and is highly associated with AAO in the mid-latitude of SH. On inter-annual time scales, the BHCM is highly correlated with ENSO. The atmosphere in both the NH and SH responds to sea surface temperature anomalies in the equatorial region, while the contemporaneous circulation changes in the NH and SH in turn affect the occurrence of El Ni觡o/La Ni觡a. In boreal winter, significant temperature and precipitation anomalies associated with the BHCM are found worldwide. Specifically, in the positive phase of the BHCM,temperature and precipitation are anomalously low in eastern China and some other regions of East Asia. These results are helpful for us to better understand interactions between circulations in the NH and SH and the dynamical mechanisms behind these interactions.

The Research on the Psychological Tactic of the Northern and Southern Dynasties

Psychology’s military use was discovered by ancient Chinese strategists in a very early time.In the Spring and Autumn period(770 B.C.-476 B.C.),psychological tactics were put into practice.After coming to the Northern and Southern Dynasties(420-589),psychological tactics had become the required course of strategists.Therefore,many generals of this period could use it adroitly.There were many classic examples which could help us analyze the psychological tactics of this era.The purpose of this paper is to introduce a few classic examples and summarize their characteristics.Then,we can better understand the psychological tactics of the Northern and Southern Dynasties.

Permo–Triassic and Liassic Tethyan Oceanic Tracts within the Pontide Belt Along the Southern Margin of Eurasia, Northern Anatolia

The Pontide belt in northern Turkey includes three major tectonic terranes,the Strandja Massif（Sj M）,and the Istanbul（ISZ）and Sakarya Zones（SZ）(Fig.1).We present new age and geochemical data from ophiolites and ophiolitic mélanges within the Sakarya Zone and show that these mafic–ultramafic rocks are the remnants of Tethyan oceanic lithosphere formed in different tectonic settings.The main ophiolite occurrences investigated in this study along the Karakaya Suture（KS）are associated with the latest Triassic Cimmeride orogeny,and in the Küre–Yusufeli ophiolite belt are part of the Alpide orogeny.The Karakaya Suture Zone ophiolites in northern west Turkey are comprised mainly of the Denizgoren（?anakkale）ophiolite,Bo?azk?y（Bursa）,Geyve（Sakarya）,Almac?k（Düzce）and?ele（Bolu）metaophiolites.The Denizg?ren ophiolite largely contains upper mantle peridotites,which are equivalents of the Permo–Triassic Lesvos peridotites and mélange units farther SW in the northern Aegean Sea.The Bo?azk?y ophiolite includes serpentinite and metagabbro,and the Almac?k and Geyve ophiolites display an almost complete Penrose–type sequence consisting of serpentinizeduppermantleperidotites,cumulate ultramafic–mafic rocks,isotropic gabbros,dolerite and plagiogranite dikes,and extrusive rocks.U–Pb zircon dating of plagiogranite dikes from?ele has revealed an igneous age of 260 Ma,and 255,235,227 Ma from Almac?k（Bozkurt et al.,2012a,b）.Consistent with the previouslypublished Permo–Triassic age,we obtained a 268.4±6.3 Ma U–Pb zircon age from a plagiogranite dike within the Almacik ophiolite to the west.This KS ophiolite belt containing the?ele,Almac?k,Geyve ophiolites within the SZ extends westward into the Armutlu Peninsula and then into the Biga Peninsula（i.e.Denizg?ren ophiolite）and most likely connects with the remnants of the Triassic Meliata–Meliac ocean basin（Stampfli and Borel,2002）in the Balkan Peninsula.The KS ophiolites also continue eastward within the Pontide Belt into the Elekda?ophiolite（eastern Kastamonu）and then to the Refahiye ophiolite in NE Anatolia.Triassic granites in the SZ represent a magmatic arc that formed as a result of the northward subduction of the Izmir–Ankara–Erzincan oceanic lithosphere existing during the late Paleozoic through Cretaceous（Sarifakioglu et al.,2014）beneath the Pontides.We obtained a U–Pb zircon age of 231±2 Ma from a metagranitic intrusion into the Variscan basement of the SZ in the Kastamonu region of the central Pontides.This metagranite is enriched in LILE（Rb:63 ppm;Ba:65 ppm;Sr:200 ppm）and depleted in HFSE（Y:12.58 ppm;Yb:1.26 ppm;Ti O2:0.2 wt.%;Nb:7.6 ppm;Hf:3.9 ppm）,characterizing it as subduction–related calc-alkaline pluton.Lead（3.9 ppm）,U（1.6 ppm）and Ce（59 ppm）contents are interpreted as evidence for contamination by continental crust.The Küre basin to the north opened during the late Triassic to Liassic,following a backarc rifting episode in the central Pontides.Metabasic dike intrusions in the Devrekani metamorphic massif represent the first magmatic stage of this backarc rifting event.Whole-rock 40Ar-39Ar dating ofthe metabasic dikes has yielded cooling ages of 160.5±1.2 Ma. We infer that this age was reset due to thermal heating during the emplacement of the Middle Jurassic granitoids as the Küre oceanic basin was closing. The Küre ophiolite contains upper mantle peridotites and oceanic crustal rocks composed mainly of pillow–massive–breccia basalts, dacitic and rhyolitic lavas–tuffs, diabase dyke swarms, massive gabbros and a limited extent mafic cumulates. We obtained 182.6±1.9 Ma as a whole-rock 40Ar-39 Ar age from a pillow basalt and a U–Pb zircon age of 171±1 Ma from the granitic intrusion cross-cutting the peridotites. The easternmost representatives of the Küre ophiolite occur in the Yusufeli（Artvin） area in the eastern part of the Pontide belt. Here, oceanic crustal rocks are tectonically related to metamorphic rocks of the Variscan basement of the SZ. The ophiolitic crustal rocks contain isotropic gabbro and mafic and felsic dikes. Serpentinized upper mantle peridotites are scarce. Pillow lava basalts are overlain by a thick metasandstone–metashale association with locally foliated meta–lava and some manganiferous chert and mudstone interlayers. We obtained a U–Pb zircon age of 172.5±1.4 Ma from the granitic intrusion cross-cutting the Yusufeli ophiolite and of 181.9±0.9 Ma from a felsic dike（plagiogranite） in the Yusufeli ophiolite. The Middle Jurassic granites are related to the closure of the Küre-Yusufeli marginal ocean basin. The Küre and Yusufeli ophiolites have been previously interpreted as the remnants of the Paleotethys or the Intra-Pontide Ocean. However, we posit that these ophiolites represent amarginal, short-lived（;0 Ma） ocean basin, which opened during the late Triassic through Liassic, and then closed in Dogger. This oceanic lithosphere is similar to the Evros ophiolite in the northeastern Greece in terms of its ages and geochemical characteristics.

Zircon U-Pb dating,Geochemical Features of the Halunwusu Composite Granites at the Northern Edge of Southern Qilian,Qinghai

1 Introduction Halunwusu Composite Granites,locates in the northern margin of Belt,between the North Qaidam block and the Central Qilian block,with two phases of magmatic activities.The granites mainly consisits of the Early

Analysis of Buddhist sculpture art in Wei, Jin, southern and Northern Dynasties

The emergence and development of Buddhist sculpture has completely changed the original Chinese ancient sculpture, rich China sculpture theme, greatly promote the development of the Chinese sculpture, in the Wei and Jin Dynasties, Buddhist sculpture received considerable development, the art form is abundant, representative of the ＂ slim but clear appearance＂ and＂ images of noble figures ＂two kinds of modeling features. From the perspective of historical background and aesthetic orientation, this paper analyzes and studies the overall features of the art of carving art in this historical period, as well as its profound influence on the development of Chinese Buddhist statues.

Analysis on the Homogeneities between Traditional Chinese Music and Painting Arts from Qichang Dong＇s ＂Theory of Southern and Northern Schools＂

Qichang Dong was a famous calligraphy and painting artist in ancient China. The theory of southern and northern schools proposed by him was a conclusion to traditional Chinese landscape painting art, and played an incomparable function in the traditional Chinese painting history in comparison with other painting theories. In the painting history, there were the theory of ＂pre-Dong＂ and the theory of ＂post-Dong＂ . In this paper, by starting from Qichang Dong＇ s ＂theory of southern and northern schoots＂ , the homogeneities between traditional Chinese music and painting arts are analyzed from the principle of spirit and the principle of technology, so that the homogeneities between traditional Chinese music and painting arts are explored in aesthetic concept and actual art creation.

Metamorphic Evolution of Eclogite Facies Rocks in Northern Hubei and Southern Henan,China

Eclogite facies rocks in this area are diverse in rock type. The field occurrence androck-chemistry reflect their in-situ origin. Based on their regional geology and field occurrence,two groups of eclogites are divided in terms of their peak temperature of metamorphism. Medi-um-temperature eclogites (MT), as Group B, occur in the Dabie Group. They were formedfrom epidote-amphibolite facies. The metamorphism of eclogite facies has two stages: the co-esite eclogite facies stage (the peak condition: T = 600-700℃, P = 2. 7 - 3. 0 GPa) and theglaucophane eclogite facies stage (the pressure decreases, may be lower than 2. 5 GPa while thetemperature has little change). Low temperature eclogites (LT), as Group C, occur in the Qi-jiaoshan Formation. They were formed from blue schist facies (the peak condition: T = 490 -560℃, P< 1. 5 GPa). The appearance of hydrous minerals in the eclogites indicates the im-portant role of water in metamorphism. Medium-temperature eclogites are different from low-temperature ones in metamorphism. At last, the evolution of the high-pressure metamorphicbelt is discussed as well.

沁水盆地南部中深部煤层气储层特征及开发技术对策

为了实现沁水盆地南部中深部煤层气高效开发,以郑庄北-沁南西区块为研究对象,基于参数井取心分析测试、注入/压降测试、地应力循环测试结果和大量动静态数据,通过与浅部对比,阐述了中深部煤储层特征,分析了从浅部到中深部煤层直井压裂和水平井分段压裂两种开发技术的改进,进而提出了中深部煤层气主体开发技术。结果表明,郑庄北-沁南西区块3号煤平均埋深1200 m左右,为中深部煤层气储层。随着埋深增加,研究区含气量和吸附时间均先增加后降低,含气量和吸附时间峰值分别位于埋深1100~1200 m和800~1000 m;随着埋深增加,研究区地应力场类型发生了2次转换,埋深小于600 m时,为逆断层型地应力场类型,水力压裂易形成水平缝,利于造长缝;埋深大于1000 m时为走滑断层型地应力场类型,水力压裂易形成垂直缝,裂缝延伸较短;埋深为600~1000 m时,地应力场由逆断层型向走滑断层型转换阶段,水力压裂形成的裂缝系统较为复杂。与浅层相比,中深部储层含气量、解吸效率和应力场发生明显转变。随着埋深增加,无论是直井(定向井)还是水平井,均应采用更大的压裂规模才能获得较好的效果。对于直井,埋深大于800 m后,压裂液量达到1500 m^(3)以上、排量12~15 m^(3)/min以上、砂比10%~14%以上,单井日产气量可以达到1000 m^(3)以上;对于水平井,埋深大于800 m后,压裂段间距控制在70~90 m以下,单段液量、砂量分别达到2000、150 m^(3)以上,排量达到15 m^(3)/min以上开发效果较好,单井产量突破18000 m^(3)。随着埋深增加,水平井开发方式明显优于直井,以二开全通径水平井井型结构、优质层段识别技术和大规模、大排量缝网压裂为核心的水平井开发方式是适用于沁水盆地南部中深部煤层气高效开发的主体工艺技术。

南稻北麦与公司违规——基于水稻理论的实证研究

利用2003—2018年中国上市公司违规行为的数据,研究了水稻种植与公司违规之间的联系及其内在机制。研究表明,随着CEO籍贯地种植水稻的比例提高,公司违规的概率也相应增大,稻作经济所带来的低社会信任度和风险偏好文化是其传导机制。儒家文化影响力与稻作文化在影响公司违规方面存在一定的替代关系,CEO学历的提高能在一定程度上抑制稻作文化对公司违规的正向影响。基于舞弊三角理论,检验了在不同压力与机会要素下,水稻种植的异质性影响,发现CEO籍贯地水稻种植比例在经营压力小、违规机会大的公司能够对公司违规起到更大影响。

从体部指数值探讨中国族群体部特征的共性

目的探讨中国族群体部特征的共性。方法对中国62个族群的8项体部指数值进行分析。结果聚类分析发现,男性52个族群、女性59个族群聚入了北方族群为主的混合组及南方族群为主的混合组。8个汉族族群分别聚入各个组中,没有聚合出汉族组。体部指数的分型结果表明,长躯干型、中胸型、宽肩型、宽骨盆型和中腿型是中国男性族群主要的体部类型。中躯干型、宽胸型、宽肩型、宽骨盆型和中腿型是中国女性族群主要的体部类型。这表现出中国各族群体部特征具有明显的一致性。中国族群体部特征的一致性与其族源相近有关,汉族在中国各个族群交流融合过程中起了重要作用。在汉族的历史上,出现过多次大规模的人口迁徙。北方少数民族南下融入北方汉族,北方汉族南下促进了南方汉族的形成,使得南方汉族与北方汉族体部特征具有相似性,也促进了南方少数民族融入南方汉族。此外,迁入少数民族地区的汉族也逐渐融入少数民族中。结论中国各族群体部特征存在明显的共性。

塑料大棚香瓜-番茄轮作生产技术

基于黑龙江省在保障粮食供给以及自然资发[2021]166号文件中严格控制农业设施建设用地使用一般耕地要求的两大背景下,开展塑料大棚一年两茬高效生产技术模式研究,形成地方标准1项,主要包括轮作茬口安排、香瓜生产技术、过渡期设施管理、番茄生产技术,以期建立以夏秋茬北菜南销番茄生产为主,早春抢早香瓜生产为辅的轮作模式,提升现有塑料大棚的产出率以及推进“北菜南销”塑料大棚番茄产业可持续发展。

气候变化影响下多年冻土区铁路路基热稳定性分析

全球气候变暖加剧了青藏高原气候暖湿化,威胁着高原铁路路基及下伏多年冻土的热稳定性,但以往研究缺乏综合考虑铁路沿线气候、多年冻土及路基稳定性的系统分析。针对这一研究的不足,基于铁路沿线气象和多年冻土路基地温监测数据,分析铁路沿线多年冻土区气温降水、天然场地年平均地温与天然上限、路基人为上限及路基左右路肩沉降变化,揭示气候暖湿化背景下铁路多年冻土路基热稳定性变化,为多年冻土区铁路建设和维护提供参考。结果表明:近20年来,铁路沿线年均气温和年均降水量的平均值分别增加了1.2℃和80mm;相较于2007年,2020年铁路沿线天然场地多年冻土年均地温平均升高0.1℃,多年冻土天然上限平均下降0.58 m,路基人为上限平均抬升2.34 m,路基左路肩平均沉降大于右路肩,存在阴阳坡效应。整体而言,铁路多年冻土路基状态稳定,运行状态良好,建设运营期间采取的一系列工程措施有效,但面向未来气候加剧变化趋势,应提前谋划多年冻土保护新技术。