The article Jetting-based bioprinting:process,dispense physics,and applications,written by Wei Long Ng and Viktor Shkolnikov,was originally published electronically on the publisher’s internet portal on 12 July 2024 ...The article Jetting-based bioprinting:process,dispense physics,and applications,written by Wei Long Ng and Viktor Shkolnikov,was originally published electronically on the publisher’s internet portal on 12 July 2024 without open access.展开更多
Sea pens are a highly specialized and morphologically distinct group of octocorallians,but many taxa were poorly described and their phylogenetic relationships are still poorly known.We describe two species of sea pen...Sea pens are a highly specialized and morphologically distinct group of octocorallians,but many taxa were poorly described and their phylogenetic relationships are still poorly known.We describe two species of sea pens collected from the tropical Western Pacific:Alloptilella splendida gen.et sp.nov.and Scytalium veneris(Thomson&Henderson,1906).Alloptilella splendida gen.et sp.nov.accords with the definition of the recently resurrected genus Ptilella Gray,1870 in most characters,but differs from the latter in the arrangement of autozooids and the location of mesozooids.The new species mostly resembles Pennatula naresi K(o|")lliker(1880),but differs by the location of me sozooids and the reddish color of sclerite s surrounding siphonozooids.The generic separation is also well supported by their genetic distances and the molecular phylogenetic trees constructed by the concatenated mtMutS-COI-28 S,where Alloptilella clustered with Scytalium Herklots,1858 and formed a clade with the cluster of Ptilella and Distichoptilum.Scytalium veneris,usually considered as a junior synonym of Scytalium sarsii Herklots,1858,is resurrected by recognizing their distinctive differences.This is the first record of Scytalium veneris outside its type locality,and the phylogenetic analysis indicates that Scytalium is a monophyletic group.Both the families Pennatulidae and Virgulariidae are polyphyletic,and more morphological and molecular data are needed to clarify the phylogenetic positions of pennatulacean families and genera.展开更多
[ Objective ] The paper aimed to screen the poison bait and lure suitable for controlling Apodemus peninsulae. [ Method ] A. peninsulae was reared individually in a single cage, to observe its feeding conditions. [Res...[ Objective ] The paper aimed to screen the poison bait and lure suitable for controlling Apodemus peninsulae. [ Method ] A. peninsulae was reared individually in a single cage, to observe its feeding conditions. [Result] A. peninsulae preferred carrot and cucumber, followed by peanut, and rat diet was the last choice. [ Conclusion] Carrot and cucumber could be used to prepare poisoning bait for controlling A. peninsulae, in order to improve rat capture efficiency.展开更多
A starvation trial was conducted to determine compensatory growth of the juvenile golden pompano Trachinotus ovatus which were deprived of food for 1, 2, 5 and 7 days respectively. The results of tests in outdoor ceme...A starvation trial was conducted to determine compensatory growth of the juvenile golden pompano Trachinotus ovatus which were deprived of food for 1, 2, 5 and 7 days respectively. The results of tests in outdoor cement pools and net pens showed that, in the first 15 days and 30 days, the growth of 1 day and 2 days-deprived groups was probably the same with that of control group, which accorded with fully compensatory description. However, the weight of 5 days and 7 days-deprived fingerlings were lower than that of the control group, which accorded with the description of the partial compensatory growth. As the starvation prolonged, feed conversion efficiency of the starved groups increased and was higher than the control group. Meanwhile, feeding rate also increased, but the fish of 1 day and 2 days-deprived groups was lower than that of the control group, and feeding quantity reduced and was lower than that of the control group. It suggested that the mechanism of compensatory growth was mainly due to improving feed conversion efficiency. Fish biochemical composition was analyzed: the water content and ash of fish sample increased due to starvation, while the lipid and protein decreased. The loss of lipid was greater than that of the protein, and the biochemical composition of fish in each group was restored to the control level by the end of the experiment. It suggested that Trachinotus ovatus may mainly consume lipid during the period of starvation.展开更多
In the White sturgeon fish farms, some individuals have difficulty in getting access to food: such sturgeons are called "runts", and they result in a slower growth rate than normally feeding fish. In this pa...In the White sturgeon fish farms, some individuals have difficulty in getting access to food: such sturgeons are called "runts", and they result in a slower growth rate than normally feeding fish. In this paper, we have studied the gut peculiarities of runt sturgeons. Utilizing in paralleling an analysis of diatom populations in both the fish gut tissues and the rearing tank waters, we hypothesized a causative relation between the occurrence of runt sturgeons and periodic diatom blooms. In fact, we have observed that the diatom species identified in the aquatic environment were also detected in organs (Fragilaria spp and Rhoicosfenia spp for both glandular body, mid-intestine) of the runt sturgeon's gut, but not in tissues of normally feeding individuals. Owing to their siliceous wall, diatoms can be responsible for areas of epithetlial detachment in the mucosal surfaces of the alimentary canal and a catharral inflammation in both the gastric pits and intestinal folds which may be the cause of secondary bacterial diseases. We suggest that diatom blooms may contribute to the occurrence of runt sturgeons in the studied Italian fish farm.展开更多
A high rate of improper antibiotic dispensing was reported to patients with presumed viral infections even without a prescription from a physician in Vietnam[1]. Imprudent antibiotic dispensing and use were also obser...A high rate of improper antibiotic dispensing was reported to patients with presumed viral infections even without a prescription from a physician in Vietnam[1]. Imprudent antibiotic dispensing and use were also observed in other countries, including the Philippines. A large number of patients experienced respiratory symptoms, such as cough and rhinorrhea during the COVID-19 pandemic, which exacerbated this problem with antibiotic use.展开更多
Water-Soluble Chitosan(WSC)has been sucessfuly synthesized from squid pens waste.The synthesis of chitosan from chitin was carried out by optimization of deacetylation temperature and time.Chitin was obtained from squ...Water-Soluble Chitosan(WSC)has been sucessfuly synthesized from squid pens waste.The synthesis of chitosan from chitin was carried out by optimization of deacetylation temperature and time.Chitin was obtained from squid pens waste by demineralization and deproteinization process.HCl 7%was used for demineralization and NaOH 10%at 60℃ was applied for deproteinization process.Deacetylation reaction was carried out at varied temperatures i.e.,60℃,70℃,80℃,90℃ and 100℃ in NaOH 50%solution for 10 hours.Deacetylation reaction time were varied for 2 hours,4 hours,6 hours,8 hours,and 10 hours.The crude chitosan obtained then reacted with H2O230%to depolymerize.The synthesis product obtained then characterized by FTIR.The result of squid chitin yield was 33.9%.The optimum temperature and time of chitosan deacetylation process were 90℃ for 8 hours as indicated by the value of deacetylation degree(DD)that equal to 83.94%at optimum temperature and 82.22%at optimum reaction time.The percentage of WSC yield at optimum temperature(90℃)and optimum time(8 hours)were 27.59%and 23.16%,respectively.WSC solubility test was done in water and HCl 0,1N.The solubility of 2.8325 mg/mL and 0.8125 mg/mL were obtained in acid medium and water medium,respectively.展开更多
During the Eighth Five-Year Plan period, (1991-95), China’s pen industry maintained sustained, rapid, stable and sound development with the further promotion of product quality and grade in production, and its techno...During the Eighth Five-Year Plan period, (1991-95), China’s pen industry maintained sustained, rapid, stable and sound development with the further promotion of product quality and grade in production, and its technological advancement. At the same time, new varieties of products and more patterns and specifications were developed. The industry also further developed its business ties with clients abroad and ex-展开更多
The use of natural enemies represents an important strategy in programs of Integrated Pest Management (IPM). Asopinae predators are reared with alternative prey, but supplementary plant may enhance the fecundity and l...The use of natural enemies represents an important strategy in programs of Integrated Pest Management (IPM). Asopinae predators are reared with alternative prey, but supplementary plant may enhance the fecundity and longevity of their females. The objective of this research was to evaluate the development and reproduction of the predator Brontocoris tabidus Signoret, 1852 (Heteroptera: Pentatomidae) fed with Tenebrio molitor L., 1785 (Coleoptera: Tenebrionidae) pupae in a Eucalyptus grandis Hill ex Maiden (Myrtaceae) plant in the field. The duration and survival of the nymphal stage of B. tabidus were 32.07 ± 8.95 days and 77.00 ± 0.42%, respectively with 15.55 ± 8.19 egg masses, 603.00 ± 379.20 eggs and 387.45 ± 291.40 nymphs per female of this predator while longevity of its males and females was 119.80 ± 32.80 and 160.20 ± 42.13 days, respectively. Better results with E. grandis plant in the field and the fact that B. tabidus will be adapted at release time show that this predator should be reared in these conditions.展开更多
The debris cloud generated by the hypervelocity impact(HVI)of orbiting space debris directly threatens the spacecraft.A full understanding of the damage mechanism of rear plate is useful for the optimal design of prot...The debris cloud generated by the hypervelocity impact(HVI)of orbiting space debris directly threatens the spacecraft.A full understanding of the damage mechanism of rear plate is useful for the optimal design of protective structures.In this study,the hypervelocity yaw impact of a cylindrical aluminum projectile on a double-layer aluminum plate is simulated by the FE-SPH adaptive method,and the damage process of the rear plate under the impact of the debris cloud is analyzed based on the debris cloud structure.The damage process can be divided into the main impact stage of the debris cloud and the structural response of the rear plate.The main impact stage lasts a short time and is the basis of the rear plate damage.In the stage of structure response,the continuous deformation and inertial motion of the rear plate dominate the perforation of the rear plate.We further analyze the damage mechanism and damage distribution characteristics of the rear plate in detail.Moreover,the connection between velocity space and position space of the debris cloud is established,which promotes the general analysis of the damage law of debris cloud.Based on the relationship,the features of typical damage areas are identified by the localized fine analysis.Both the cumulative effect and structural response cause the perforation of rear plate;in the non-perforated area,cratering by the impact of hazardous fragments is the main damage mode of the rear plate.展开更多
【目的】Pen a 1是虾中的主要过敏原,已知其5个抗原表位在过敏反应中起着决定性的作用。用全蛋白抗体和表位特异性抗体,检测Pen a 1经体外模拟胃液(simulated gastric fluid,SGF)消化后其抗原表位免疫原性的变化情况,为研究人体消化对Pe...【目的】Pen a 1是虾中的主要过敏原,已知其5个抗原表位在过敏反应中起着决定性的作用。用全蛋白抗体和表位特异性抗体,检测Pen a 1经体外模拟胃液(simulated gastric fluid,SGF)消化后其抗原表位免疫原性的变化情况,为研究人体消化对Pen a 1的影响机理及脱敏食品的制备提供理论依据。【方法】以刀额新对虾(metapenaeus ensis)为原材料,提取纯化虾过敏原Pen a 1,采用Fmoc化学法合成Pen a 1抗原表位的5个多肽片段(No.1—No.5),分别与载体蛋白KLH和牛血清蛋白BSA偶联制备人工免疫原和包被原。用Pen a 1和制备的多肽免疫原免疫新西兰纯种白兔获得全白蛋抗体和特异性表位抗体。参照美国药典模拟人体胃液的条件消化处理Pen a 1,利用SDS-PAGE和Tricine-SDS-PAGE观察消化后Pen a 1分子量变化;通过免疫印迹(Western-blot)定性观察Pen a 1消化后生成的新蛋白片段与各抗体的结合情况;再经竞争抑制酶联免疫吸附试验(ci-ELISA)定量检测消化后的Pen a 1和表位多肽与各抗体结合能力,从而得出Pen a 1经SGF消化后各抗原表位免疫原性的变化程度。【结果】SDS-PAGE结果显示Pen a 1随SGF消化时间的延长逐渐被降解,在22 kD处生成一些较稳定的新蛋白片段,Tricine-SDS-PAGE结果表明在1.7—18 k D之间无新蛋白片段生成。Western-blot表明消化后分解生成的小分子蛋白与六种抗体发生不同程度结合,其中全蛋白抗体和No.4抗体几乎与生成的所有小分子蛋白结合,在22 k D左右处生成的稳定新蛋白片段均与No.3、4、5抗体有较强的结合,而与No.1、2抗体结合较弱甚至无结合,表明该蛋白携带No.3、4、5表位,而基本无No.1、2表位。ci-ELISA结果显示,Pen a 1经SGF消化后对全蛋白抗体和5个表位抗体的抑制率均显著下降,说明经过消化后Pena1及其抗原表位的免疫原性均明显降低,各抗原表位消化稳定性排序为No.4>No.2>No.1>No.3>No.5,其中No.2、1、3之间无显著性差异;ci-ELISA检测表位多肽经消化后其免疫原性也显著下降,消化稳定性排序为No.1>No.2>No.3>No.4>No.5,其中No.2、3、4之间无显著性差异。结合免疫印迹结果可知No.4抗原表位消化稳定性最高,但其表位多肽消化稳定性没有显著高于其它所有表位多肽,说明在消化过程中Pen a 1结构对该表位起到一定的保护作用,使其保持较高的消化稳定性;No.1、2、3次之,3个表位间无显著性差异;No.5消化稳定性最差,表明Pen a 1空间结构对其无明显保护作用。【结论】建立了一种用表位抗体检测过敏原经模拟消化后抗原表位消化稳定性的方法。Pen a 1经SGF消化后,免疫原性降低,分解生成部分较稳定的蛋白片段,但仍具有致敏性;5个抗原表位中No.4消化稳定最高,No.5最差。展开更多
文摘The article Jetting-based bioprinting:process,dispense physics,and applications,written by Wei Long Ng and Viktor Shkolnikov,was originally published electronically on the publisher’s internet portal on 12 July 2024 without open access.
基金the Science&Technology Basic Resources Investigation Program of China(No.2017FY100804)the National Natural Science Foundation of China(Nos.41930533,31970489)+1 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDB42000000)the Senior User Project of R/V Kexue(Nos.KEXUE2019GZ04,KEXUE2020G01)。
文摘Sea pens are a highly specialized and morphologically distinct group of octocorallians,but many taxa were poorly described and their phylogenetic relationships are still poorly known.We describe two species of sea pens collected from the tropical Western Pacific:Alloptilella splendida gen.et sp.nov.and Scytalium veneris(Thomson&Henderson,1906).Alloptilella splendida gen.et sp.nov.accords with the definition of the recently resurrected genus Ptilella Gray,1870 in most characters,but differs from the latter in the arrangement of autozooids and the location of mesozooids.The new species mostly resembles Pennatula naresi K(o|")lliker(1880),but differs by the location of me sozooids and the reddish color of sclerite s surrounding siphonozooids.The generic separation is also well supported by their genetic distances and the molecular phylogenetic trees constructed by the concatenated mtMutS-COI-28 S,where Alloptilella clustered with Scytalium Herklots,1858 and formed a clade with the cluster of Ptilella and Distichoptilum.Scytalium veneris,usually considered as a junior synonym of Scytalium sarsii Herklots,1858,is resurrected by recognizing their distinctive differences.This is the first record of Scytalium veneris outside its type locality,and the phylogenetic analysis indicates that Scytalium is a monophyletic group.Both the families Pennatulidae and Virgulariidae are polyphyletic,and more morphological and molecular data are needed to clarify the phylogenetic positions of pennatulacean families and genera.
基金Supported by Science and Technology Innovation Team Projects of Colleges and Universities in Heilongjiang Province(Collapse Mechanism and Control Technology Research of Forest Rodent Population)Rodent Fauna in Mudanjiang Sandaoguan Forest Farm and Their Harms on Agriculture and Forestry(yjsxscx2015-12mdjnu)
文摘[ Objective ] The paper aimed to screen the poison bait and lure suitable for controlling Apodemus peninsulae. [ Method ] A. peninsulae was reared individually in a single cage, to observe its feeding conditions. [Result] A. peninsulae preferred carrot and cucumber, followed by peanut, and rat diet was the last choice. [ Conclusion] Carrot and cucumber could be used to prepare poisoning bait for controlling A. peninsulae, in order to improve rat capture efficiency.
基金Supported by Ocean Public Welfare Scientific Research Project,State Oceanic Administration of the People's Republic of China(Grant No.201205028)Key Projects in the National Science&Technology Pillar Program during the Twelfth Five-year Plan Period(2011BD13B11)Hainan Scientific Operating Expense Projects and Major Project of Science and Technology(ZDZX2013009,ZDZX2013014)
文摘A starvation trial was conducted to determine compensatory growth of the juvenile golden pompano Trachinotus ovatus which were deprived of food for 1, 2, 5 and 7 days respectively. The results of tests in outdoor cement pools and net pens showed that, in the first 15 days and 30 days, the growth of 1 day and 2 days-deprived groups was probably the same with that of control group, which accorded with fully compensatory description. However, the weight of 5 days and 7 days-deprived fingerlings were lower than that of the control group, which accorded with the description of the partial compensatory growth. As the starvation prolonged, feed conversion efficiency of the starved groups increased and was higher than the control group. Meanwhile, feeding rate also increased, but the fish of 1 day and 2 days-deprived groups was lower than that of the control group, and feeding quantity reduced and was lower than that of the control group. It suggested that the mechanism of compensatory growth was mainly due to improving feed conversion efficiency. Fish biochemical composition was analyzed: the water content and ash of fish sample increased due to starvation, while the lipid and protein decreased. The loss of lipid was greater than that of the protein, and the biochemical composition of fish in each group was restored to the control level by the end of the experiment. It suggested that Trachinotus ovatus may mainly consume lipid during the period of starvation.
文摘In the White sturgeon fish farms, some individuals have difficulty in getting access to food: such sturgeons are called "runts", and they result in a slower growth rate than normally feeding fish. In this paper, we have studied the gut peculiarities of runt sturgeons. Utilizing in paralleling an analysis of diatom populations in both the fish gut tissues and the rearing tank waters, we hypothesized a causative relation between the occurrence of runt sturgeons and periodic diatom blooms. In fact, we have observed that the diatom species identified in the aquatic environment were also detected in organs (Fragilaria spp and Rhoicosfenia spp for both glandular body, mid-intestine) of the runt sturgeon's gut, but not in tissues of normally feeding individuals. Owing to their siliceous wall, diatoms can be responsible for areas of epithetlial detachment in the mucosal surfaces of the alimentary canal and a catharral inflammation in both the gastric pits and intestinal folds which may be the cause of secondary bacterial diseases. We suggest that diatom blooms may contribute to the occurrence of runt sturgeons in the studied Italian fish farm.
文摘A high rate of improper antibiotic dispensing was reported to patients with presumed viral infections even without a prescription from a physician in Vietnam[1]. Imprudent antibiotic dispensing and use were also observed in other countries, including the Philippines. A large number of patients experienced respiratory symptoms, such as cough and rhinorrhea during the COVID-19 pandemic, which exacerbated this problem with antibiotic use.
基金funded by Institute for Research and Community Services(LPPM,Lembaga Penelitian and Pengabdian Masyarakat)ITS(1438/PKS/ITS/2018)The research facilities were supported by Chemistry Department,Faculty Sciences,Institut Teknologi Sepuluh Nopember,Surabaya(ITS).
文摘Water-Soluble Chitosan(WSC)has been sucessfuly synthesized from squid pens waste.The synthesis of chitosan from chitin was carried out by optimization of deacetylation temperature and time.Chitin was obtained from squid pens waste by demineralization and deproteinization process.HCl 7%was used for demineralization and NaOH 10%at 60℃ was applied for deproteinization process.Deacetylation reaction was carried out at varied temperatures i.e.,60℃,70℃,80℃,90℃ and 100℃ in NaOH 50%solution for 10 hours.Deacetylation reaction time were varied for 2 hours,4 hours,6 hours,8 hours,and 10 hours.The crude chitosan obtained then reacted with H2O230%to depolymerize.The synthesis product obtained then characterized by FTIR.The result of squid chitin yield was 33.9%.The optimum temperature and time of chitosan deacetylation process were 90℃ for 8 hours as indicated by the value of deacetylation degree(DD)that equal to 83.94%at optimum temperature and 82.22%at optimum reaction time.The percentage of WSC yield at optimum temperature(90℃)and optimum time(8 hours)were 27.59%and 23.16%,respectively.WSC solubility test was done in water and HCl 0,1N.The solubility of 2.8325 mg/mL and 0.8125 mg/mL were obtained in acid medium and water medium,respectively.
文摘During the Eighth Five-Year Plan period, (1991-95), China’s pen industry maintained sustained, rapid, stable and sound development with the further promotion of product quality and grade in production, and its technological advancement. At the same time, new varieties of products and more patterns and specifications were developed. The industry also further developed its business ties with clients abroad and ex-
文摘The use of natural enemies represents an important strategy in programs of Integrated Pest Management (IPM). Asopinae predators are reared with alternative prey, but supplementary plant may enhance the fecundity and longevity of their females. The objective of this research was to evaluate the development and reproduction of the predator Brontocoris tabidus Signoret, 1852 (Heteroptera: Pentatomidae) fed with Tenebrio molitor L., 1785 (Coleoptera: Tenebrionidae) pupae in a Eucalyptus grandis Hill ex Maiden (Myrtaceae) plant in the field. The duration and survival of the nymphal stage of B. tabidus were 32.07 ± 8.95 days and 77.00 ± 0.42%, respectively with 15.55 ± 8.19 egg masses, 603.00 ± 379.20 eggs and 387.45 ± 291.40 nymphs per female of this predator while longevity of its males and females was 119.80 ± 32.80 and 160.20 ± 42.13 days, respectively. Better results with E. grandis plant in the field and the fact that B. tabidus will be adapted at release time show that this predator should be reared in these conditions.
基金supported by the Innovative Research Groups of the National Natural Science Foundation of China(Grant No.12221002)。
文摘The debris cloud generated by the hypervelocity impact(HVI)of orbiting space debris directly threatens the spacecraft.A full understanding of the damage mechanism of rear plate is useful for the optimal design of protective structures.In this study,the hypervelocity yaw impact of a cylindrical aluminum projectile on a double-layer aluminum plate is simulated by the FE-SPH adaptive method,and the damage process of the rear plate under the impact of the debris cloud is analyzed based on the debris cloud structure.The damage process can be divided into the main impact stage of the debris cloud and the structural response of the rear plate.The main impact stage lasts a short time and is the basis of the rear plate damage.In the stage of structure response,the continuous deformation and inertial motion of the rear plate dominate the perforation of the rear plate.We further analyze the damage mechanism and damage distribution characteristics of the rear plate in detail.Moreover,the connection between velocity space and position space of the debris cloud is established,which promotes the general analysis of the damage law of debris cloud.Based on the relationship,the features of typical damage areas are identified by the localized fine analysis.Both the cumulative effect and structural response cause the perforation of rear plate;in the non-perforated area,cratering by the impact of hazardous fragments is the main damage mode of the rear plate.
文摘【目的】Pen a 1是虾中的主要过敏原,已知其5个抗原表位在过敏反应中起着决定性的作用。用全蛋白抗体和表位特异性抗体,检测Pen a 1经体外模拟胃液(simulated gastric fluid,SGF)消化后其抗原表位免疫原性的变化情况,为研究人体消化对Pen a 1的影响机理及脱敏食品的制备提供理论依据。【方法】以刀额新对虾(metapenaeus ensis)为原材料,提取纯化虾过敏原Pen a 1,采用Fmoc化学法合成Pen a 1抗原表位的5个多肽片段(No.1—No.5),分别与载体蛋白KLH和牛血清蛋白BSA偶联制备人工免疫原和包被原。用Pen a 1和制备的多肽免疫原免疫新西兰纯种白兔获得全白蛋抗体和特异性表位抗体。参照美国药典模拟人体胃液的条件消化处理Pen a 1,利用SDS-PAGE和Tricine-SDS-PAGE观察消化后Pen a 1分子量变化;通过免疫印迹(Western-blot)定性观察Pen a 1消化后生成的新蛋白片段与各抗体的结合情况;再经竞争抑制酶联免疫吸附试验(ci-ELISA)定量检测消化后的Pen a 1和表位多肽与各抗体结合能力,从而得出Pen a 1经SGF消化后各抗原表位免疫原性的变化程度。【结果】SDS-PAGE结果显示Pen a 1随SGF消化时间的延长逐渐被降解,在22 kD处生成一些较稳定的新蛋白片段,Tricine-SDS-PAGE结果表明在1.7—18 k D之间无新蛋白片段生成。Western-blot表明消化后分解生成的小分子蛋白与六种抗体发生不同程度结合,其中全蛋白抗体和No.4抗体几乎与生成的所有小分子蛋白结合,在22 k D左右处生成的稳定新蛋白片段均与No.3、4、5抗体有较强的结合,而与No.1、2抗体结合较弱甚至无结合,表明该蛋白携带No.3、4、5表位,而基本无No.1、2表位。ci-ELISA结果显示,Pen a 1经SGF消化后对全蛋白抗体和5个表位抗体的抑制率均显著下降,说明经过消化后Pena1及其抗原表位的免疫原性均明显降低,各抗原表位消化稳定性排序为No.4>No.2>No.1>No.3>No.5,其中No.2、1、3之间无显著性差异;ci-ELISA检测表位多肽经消化后其免疫原性也显著下降,消化稳定性排序为No.1>No.2>No.3>No.4>No.5,其中No.2、3、4之间无显著性差异。结合免疫印迹结果可知No.4抗原表位消化稳定性最高,但其表位多肽消化稳定性没有显著高于其它所有表位多肽,说明在消化过程中Pen a 1结构对该表位起到一定的保护作用,使其保持较高的消化稳定性;No.1、2、3次之,3个表位间无显著性差异;No.5消化稳定性最差,表明Pen a 1空间结构对其无明显保护作用。【结论】建立了一种用表位抗体检测过敏原经模拟消化后抗原表位消化稳定性的方法。Pen a 1经SGF消化后,免疫原性降低,分解生成部分较稳定的蛋白片段,但仍具有致敏性;5个抗原表位中No.4消化稳定最高,No.5最差。