Founded in 1919, Shanghai Hua Tong Switchgear Works ("HTSW" in short for below) is one of large enterprises on state level. With high and new technology, it is designed as an export base for mechanical and e...Founded in 1919, Shanghai Hua Tong Switchgear Works ("HTSW" in short for below) is one of large enterprises on state level. With high and new technology, it is designed as an export base for mechanical and electrical product of the Ministry of Machinery Industry as well. It has been awarded the honor of first grade factory in the competition for key and major project construction in Shanghai year by year,展开更多
Objective:To investigate the mechanism of the effect of Daodi Tongguan Decoction on ovarian function in rats with premature ovarian failure.Methods:Forty SD female rats with regular estrous cycle were randomly divided...Objective:To investigate the mechanism of the effect of Daodi Tongguan Decoction on ovarian function in rats with premature ovarian failure.Methods:Forty SD female rats with regular estrous cycle were randomly divided into the blank group,the model group,the positive group(Bujiale 0.099 mg/kg),the Daodigitongguan decoction high-dose group(1.4 g/100 g)and the Daodigitongguan decoction medium-dose group(0.7 g/100 g).The model group,positive group,Daodi Tongguan Decoction high-dose group and Daodi Tongguan decoction medium-dose group were given Tripterygium wilfordii polyglycoside tablets suspension(50 mg/kg)by continuous gavage for 14 days to establish a rat model of premature ovarian failure.After the modeling,each group was given the corresponding intervention agent for 28 consecutive days.After the intervention,serum and ovarian tissue were collected from rats in each group.The morphological structure of rat ovarian tissue was observed after HE staining.Serum AMH level was determined by ELISA.The expressions of Bcl-2,Bax and VEGF in ovarian tissue were detected by IHC.The apoptosis of granulosa cells in ovary was observed by TUNEL.Results:Compared to the normal group,the growth follicles in the model group were reduced and the atretic follicles were increased.Serum AMH level was significantly decreased(P<0.01).The expression of Bax protein in ovarian tissue was increased(P<0.01).The protein expressions of Bcl-2 and VEGF were decreased(P<0.01,P<0.05),and the apoptosis rate of follicular granulosa cells in ovarian tissue was increased(P<0.01).Compared to the molding group,the serum AMH level in the TCM dose groups was increased to varying degrees,and the change of the AMH in high-dose group was the most significant(P<0.05).The expression of Bax protein in ovarian tissue decreased(P<0.05),while the expression of Bcl-2 and VEGF protein increased(P<0.05,P<0.05).The apoptosis rate of follicular granulosa cells in ovarian tissue was decreased in the high dose group(P<0.05).Conclusion:Daodi Tongguan Decoction can improve the histological morphology of ovary and relieve ovarian injury,and its mechanism may be related to improving the sex hormone level of POF model rats,reducing the expression level of Bax protein in ovarian tissue,increasing the expression level of Bcl-2 and VEGF protein in ovarian tissue,and reducing the apoptosis rate of follicular granulosa cells in ovarian tissue.展开更多
THE traditional Chongyanag Festival-also known as the Festival of the Elderly-falls on the ninth day of the ninth month of the lunar calendar.Legend has it that during the Eastern Han Dynasty(25-220),there was a Taois...THE traditional Chongyanag Festival-also known as the Festival of the Elderly-falls on the ninth day of the ninth month of the lunar calendar.Legend has it that during the Eastern Han Dynasty(25-220),there was a Taoist named Huanjing,whose hometown,Shangcai,now a county in north China’s Henan Province,was ravaged by plague and people were living in miserable conditions.On the ninth day of the ninth lunar month,Huanjing brought dogwood twigs and chrysanthemum liquor back home to relieve his hometown of its suffering.展开更多
文摘Founded in 1919, Shanghai Hua Tong Switchgear Works ("HTSW" in short for below) is one of large enterprises on state level. With high and new technology, it is designed as an export base for mechanical and electrical product of the Ministry of Machinery Industry as well. It has been awarded the honor of first grade factory in the competition for key and major project construction in Shanghai year by year,
基金National Natural Science Foundation of China(No.81360594)Autonomous Region Traditional Chinese Medicine Inheritance and Development Special Fund Project‑Luo Zhijuan Guangxi Famous Traditional Chinese Medicine Inheritance Studio Construction Project(No.[2022]2)。
文摘Objective:To investigate the mechanism of the effect of Daodi Tongguan Decoction on ovarian function in rats with premature ovarian failure.Methods:Forty SD female rats with regular estrous cycle were randomly divided into the blank group,the model group,the positive group(Bujiale 0.099 mg/kg),the Daodigitongguan decoction high-dose group(1.4 g/100 g)and the Daodigitongguan decoction medium-dose group(0.7 g/100 g).The model group,positive group,Daodi Tongguan Decoction high-dose group and Daodi Tongguan decoction medium-dose group were given Tripterygium wilfordii polyglycoside tablets suspension(50 mg/kg)by continuous gavage for 14 days to establish a rat model of premature ovarian failure.After the modeling,each group was given the corresponding intervention agent for 28 consecutive days.After the intervention,serum and ovarian tissue were collected from rats in each group.The morphological structure of rat ovarian tissue was observed after HE staining.Serum AMH level was determined by ELISA.The expressions of Bcl-2,Bax and VEGF in ovarian tissue were detected by IHC.The apoptosis of granulosa cells in ovary was observed by TUNEL.Results:Compared to the normal group,the growth follicles in the model group were reduced and the atretic follicles were increased.Serum AMH level was significantly decreased(P<0.01).The expression of Bax protein in ovarian tissue was increased(P<0.01).The protein expressions of Bcl-2 and VEGF were decreased(P<0.01,P<0.05),and the apoptosis rate of follicular granulosa cells in ovarian tissue was increased(P<0.01).Compared to the molding group,the serum AMH level in the TCM dose groups was increased to varying degrees,and the change of the AMH in high-dose group was the most significant(P<0.05).The expression of Bax protein in ovarian tissue decreased(P<0.05),while the expression of Bcl-2 and VEGF protein increased(P<0.05,P<0.05).The apoptosis rate of follicular granulosa cells in ovarian tissue was decreased in the high dose group(P<0.05).Conclusion:Daodi Tongguan Decoction can improve the histological morphology of ovary and relieve ovarian injury,and its mechanism may be related to improving the sex hormone level of POF model rats,reducing the expression level of Bax protein in ovarian tissue,increasing the expression level of Bcl-2 and VEGF protein in ovarian tissue,and reducing the apoptosis rate of follicular granulosa cells in ovarian tissue.
文摘THE traditional Chongyanag Festival-also known as the Festival of the Elderly-falls on the ninth day of the ninth month of the lunar calendar.Legend has it that during the Eastern Han Dynasty(25-220),there was a Taoist named Huanjing,whose hometown,Shangcai,now a county in north China’s Henan Province,was ravaged by plague and people were living in miserable conditions.On the ninth day of the ninth lunar month,Huanjing brought dogwood twigs and chrysanthemum liquor back home to relieve his hometown of its suffering.
