This work aims to look for a simplifying surface that can represent the effect of the dual wheels on the variation of the stress and deformation state prevailing during the passage of traffic loads. This was facilitat...This work aims to look for a simplifying surface that can represent the effect of the dual wheels on the variation of the stress and deformation state prevailing during the passage of traffic loads. This was facilitated by the results of Thiam (2016) [4] obtained on the distribution of the vertical contact stress in the space described by the dual wheels. The analysis of the results of this study, on all the loading circles considered, shows that the radius loading circle equal to 0.181 m makes it possible to most probably represent the effect of the dual wheels. With this new surface, the effect of the dual wheels can be determined in 2D. The choice of this load is confirmed by a study in case of overload. Thus, the single axle with dual wheels is represented by a simplified diagram equipped on each side by a disk of radius 0.181 m. These results are obtained using a numerical simulation under Cast3M with a gravelly lateritic pavement.展开更多
Objective The detection of RNA single nucleotide polymorphism(SNP)is of great importance due to their association with protein expression related to various diseases and drug responses.At present,splintR ligase-assist...Objective The detection of RNA single nucleotide polymorphism(SNP)is of great importance due to their association with protein expression related to various diseases and drug responses.At present,splintR ligase-assisted methods are important approaches for RNA direct detection,but its specificity will be limited when the fidelity of ligases is not ideal.The aim of this study was to create a method to improve the specificity of splintR ligase for RNA detection.Methods In this study,a dualcompetitive-padlock-probe(DCPLP)assay without the need for additional enzymes or reactions is proposed to improve specificity of splintR ligase ligation.To verify the method,we employed dual competitive padlock probe-mediated rolling circle amplification(DCPLP-RCA)to genotype the CYP2C9 gene.Results The specificity was well improved through the competition and strand displacement of dual padlock probe,with an 83.26%reduction in nonspecific signal.By detecting synthetic RNA samples,the method demonstrated a dynamic detection range of 10 pmol/L-1 nmol/L.Furthermore,clinical samples were applied to the method to evaluate its performance,and the genotyping results were consistent with those obtained using the qPCR method.Conclusion This study has successfully established a highly specific direct RNA SNP detection method,and provided a novel avenue for accurate identification of various types of RNAs.展开更多
文摘This work aims to look for a simplifying surface that can represent the effect of the dual wheels on the variation of the stress and deformation state prevailing during the passage of traffic loads. This was facilitated by the results of Thiam (2016) [4] obtained on the distribution of the vertical contact stress in the space described by the dual wheels. The analysis of the results of this study, on all the loading circles considered, shows that the radius loading circle equal to 0.181 m makes it possible to most probably represent the effect of the dual wheels. With this new surface, the effect of the dual wheels can be determined in 2D. The choice of this load is confirmed by a study in case of overload. Thus, the single axle with dual wheels is represented by a simplified diagram equipped on each side by a disk of radius 0.181 m. These results are obtained using a numerical simulation under Cast3M with a gravelly lateritic pavement.
文摘Objective The detection of RNA single nucleotide polymorphism(SNP)is of great importance due to their association with protein expression related to various diseases and drug responses.At present,splintR ligase-assisted methods are important approaches for RNA direct detection,but its specificity will be limited when the fidelity of ligases is not ideal.The aim of this study was to create a method to improve the specificity of splintR ligase for RNA detection.Methods In this study,a dualcompetitive-padlock-probe(DCPLP)assay without the need for additional enzymes or reactions is proposed to improve specificity of splintR ligase ligation.To verify the method,we employed dual competitive padlock probe-mediated rolling circle amplification(DCPLP-RCA)to genotype the CYP2C9 gene.Results The specificity was well improved through the competition and strand displacement of dual padlock probe,with an 83.26%reduction in nonspecific signal.By detecting synthetic RNA samples,the method demonstrated a dynamic detection range of 10 pmol/L-1 nmol/L.Furthermore,clinical samples were applied to the method to evaluate its performance,and the genotyping results were consistent with those obtained using the qPCR method.Conclusion This study has successfully established a highly specific direct RNA SNP detection method,and provided a novel avenue for accurate identification of various types of RNAs.
