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鼠李糖乳杆菌HN001菌株水平快速定量方法的建立及应用
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作者 彭新凯 王娉 +5 位作者 武运 陈颖 曲天铭 王宇 赵晓美 郜忠川 《食品安全质量检测学报》 CAS 2024年第7期167-174,共8页
目的应用实时荧光定量聚合酶链式反应(real-time fluorescence quantitative polymerase chain reaction,qPCR),建立鼠李糖乳杆菌HN001菌株水平的快速定量检测方法。方法通过比对鼠李糖乳杆菌HN001的近缘菌株的基因组,筛选出鼠李糖乳杆... 目的应用实时荧光定量聚合酶链式反应(real-time fluorescence quantitative polymerase chain reaction,qPCR),建立鼠李糖乳杆菌HN001菌株水平的快速定量检测方法。方法通过比对鼠李糖乳杆菌HN001的近缘菌株的基因组,筛选出鼠李糖乳杆菌HN001菌株水平的特异基因,以菌株水平的特异基因为靶标设计引物及探针,优化反应体系和条件,建立鼠李糖乳杆菌HN001株水平的qPCR检测方法。对方法的特异性、灵敏度、检出限进行验证,并采用已建立的qPCR方法进行模拟添加样品和实际样品的检测。结果所建立的方法特异性强,与近缘菌株无交叉反应,鼠李糖乳杆菌HN001纯培养液检出限为10^(2)CFU/mL,灵敏度可达到650 copies/μL,可在2 h内完成样品检测。对模拟添加样品以及实际样品检测中qPCR和平板计数法结果的对数值进行显著性分析检验,两种方法的测定值结果均无显著性差异(P>0.05)。结论本研究建立的qPCR检测方法可有效应用于益生菌产品中鼠李糖乳杆菌HN001菌株水平定量检测,具有快速、灵敏、准确的特点。 展开更多
关键词 实时荧光定量聚合酶链式反应 鼠李糖乳杆菌HN001 菌株水平定量检测 益生菌产品
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朝天椒新品种黔研001的选育
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作者 张丽 李正丽 《中国蔬菜》 北大核心 2024年第3期118-120,共3页
黔研001是以自交系CZLB2009-4为母本,以HB32为父本配制而成的一代杂种。中熟,株型半直立,果实羊角形,平均果长7.2 cm,单果质量6.7 g,单株结果数122.8个。青熟果深绿色,老熟果鲜红色,定植至商品青果始收约43 d(天)。田间对白粉病、炭疽... 黔研001是以自交系CZLB2009-4为母本,以HB32为父本配制而成的一代杂种。中熟,株型半直立,果实羊角形,平均果长7.2 cm,单果质量6.7 g,单株结果数122.8个。青熟果深绿色,老熟果鲜红色,定植至商品青果始收约43 d(天)。田间对白粉病、炭疽病、病毒病的抗性强于对照单生理想52,每667 m^(2)鲜红椒产量1600 kg左右,适宜贵州地区春季种植。 展开更多
关键词 辣椒 黔研001 一代杂种
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Subplanes of PG( 2, q 3 ) and the Ruled Varieties V 2 5 of PG( 6,q )
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作者 Rita Vincenti 《Open Journal of Discrete Mathematics》 2024年第2期16-27,共12页
In this note we study subplanes of order q of the projective plane Π=PG( 2, q 3 ) and the ruled varieties V 2 5 of Σ=PG( 6,q ) using the spatial representation of Π in Σ, by fixing a hyperplane Σ ′ with a regula... In this note we study subplanes of order q of the projective plane Π=PG( 2, q 3 ) and the ruled varieties V 2 5 of Σ=PG( 6,q ) using the spatial representation of Π in Σ, by fixing a hyperplane Σ ′ with a regular spread of planes. First are shown some configurations of the affine q-subplanes. Then to prove that a variety V 2 5 of Σ represents a non-affine subplane of order q of Π, after having shown basic incidence properties of it, such a variety V 2 5 is constructed by choosing appropriately the two directrix curves in two complementary subspaces of Σ. The result can be translated into further incidence properties of the affine points of V 2 5 . Then a maximal bundle of varieties V 2 5 having in common one directrix cubic curve is constructed. 展开更多
关键词 Finite Geometry Translation planes SPREADS VARIETIES
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THE NONLINEAR STABILITY OF PLANE PARALLEL SHEAR FLOWS WITH RESPECT TO TILTED
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作者 许兰喜 关芳芳 《Acta Mathematica Scientia》 SCIE CSCD 2024年第3期1036-1045,共10页
The nonlinear stability of plane parallel shear flows with respect to tilted perturbations is studied by energy methods.Tilted perturbation refers to the fact that perturbations form an angleθ∈(0,π/2)with the direc... The nonlinear stability of plane parallel shear flows with respect to tilted perturbations is studied by energy methods.Tilted perturbation refers to the fact that perturbations form an angleθ∈(0,π/2)with the direction of the basic flows.By defining an energy functional,it is proven that plane parallel shear flows are unconditionally nonlinearly exponentially stable for tilted streamwise perturbation when the Reynolds number is below a certain critical value and the boundary conditions are either rigid or stress-free.In the case of stress-free boundaries,by taking advantage of the poloidal-toroidal decomposition of a solenoidal field to define energy functionals,it can be even shown that plane parallel shear flows are unconditionally nonlinearly exponentially stable for all Reynolds numbers,where the tilted perturbation can be either spanwise or streamwise. 展开更多
关键词 plane parallel shear flows energy method energy functional nonlinear stability Reynolds number
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Nonlinear constitutive models of rock structural plane and their applications
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作者 Wenlin Feng Shuangjian Niu +1 位作者 Chunsheng Qiao Dujian Zou 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第3期790-806,共17页
Structural planes play an important role in controlling the stability of rock engineering,and the influence of structural planes should be considered in the design and construction process of rock engineering.In this ... Structural planes play an important role in controlling the stability of rock engineering,and the influence of structural planes should be considered in the design and construction process of rock engineering.In this paper,mechanical properties,constitutive theory,and numerical application of structural plane are studied by a combination method of laboratory tests,theoretical derivation,and program development.The test results reveal the change laws of various mechanical parameters under different roughness and normal stress.At the pre-peak stage,a non-stationary model of shear stiffness is established,and threedimensional empirical prediction models for initial shear stiffness and residual stage roughness are proposed.The nonlinear constitutive models are established based on elasto-plastic mechanics,and the algorithms of the models are developed based on the return mapping algorithm.According to a large number of statistical analysis results,empirical prediction models are proposed for model parameters expressed by structural plane characteristic parameters.Finally,the discrete element method(DEM)is chosen to embed the constitutive models for practical application.The running programs of the constitutive models have been compiled into the discrete element model library.The comparison results between the proposed model and the Mohr-Coulomb slip model show that the proposed model can better describe nonlinear changes at different stages,and the predicted shear strength,peak strain and shear stiffness are closer to the test results.The research results of the paper are conducive to the accurate evaluation of structural plane in rock engineering. 展开更多
关键词 Structural plane Engineering stability ROUGHNESS Normal stress Elasto-plastic constitutive model Discrete element method
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A novel method for simulating nuclear explosion with chemical explosion to form an approximate plane wave: Field test and numerical simulation
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作者 Wei Ming Xiaojie Yang +3 位作者 Yadong Mao Xiang Wang Manchao He Zhigang Tao 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第6期2137-2153,共17页
A nuclear explosion in the rock mass medium can produce strong shock waves,seismic shocks,and other destructive effects,which can cause extreme damage to the underground protection infrastructures.With the increase in... A nuclear explosion in the rock mass medium can produce strong shock waves,seismic shocks,and other destructive effects,which can cause extreme damage to the underground protection infrastructures.With the increase in nuclear explosion power,underground protection engineering enabled by explosion-proof impact theory and technology ushered in a new challenge.This paper proposes to simulate nuclear explosion tests with on-site chemical explosion tests in the form of multi-hole explosions.First,the mechanism of using multi-hole simultaneous blasting to simulate a nuclear explosion to generate approximate plane waves was analyzed.The plane pressure curve at the vault of the underground protective tunnel under the action of the multi-hole simultaneous blasting was then obtained using the impact test in the rock mass at the site.According to the peak pressure at the vault plane,it was divided into three regions:the stress superposition region,the superposition region after surface reflection,and the approximate plane stress wave zone.A numerical simulation approach was developed using PFC and FLAC to study the peak particle velocity in the surrounding rock of the underground protective cave under the action of multi-hole blasting.The time-history curves of pressure and peak pressure partition obtained by the on-site multi-hole simultaneous blasting test and numerical simulation were compared and analyzed,to verify the correctness and rationality of the formation of an approximate plane wave in the simulated nuclear explosion.This comparison and analysis also provided a theoretical foundation and some research ideas for the ensuing study on the impact of a nuclear explosion. 展开更多
关键词 Approximate plane wave Multi-hole simultaneous blasting Chemical explosion Nuclear explosion Pressure sensor inclusion
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An 8-Node Plane Hybrid Element for StructuralMechanics Problems Based on the Hellinger-Reissner Variational Principle
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作者 Haonan Li WeiWang +1 位作者 Quan Shen Linquan Yao 《Computer Modeling in Engineering & Sciences》 SCIE EI 2024年第2期1277-1299,共23页
The finite element method (FEM) plays a valuable role in computer modeling and is beneficial to the mechanicaldesign of various structural parts. However, the elements produced by conventional FEM are easily inaccurat... The finite element method (FEM) plays a valuable role in computer modeling and is beneficial to the mechanicaldesign of various structural parts. However, the elements produced by conventional FEM are easily inaccurate andunstable when applied. Therefore, developing new elements within the framework of the generalized variationalprinciple is of great significance. In this paper, an 8-node plane hybrid finite element with 15 parameters (PHQ8-15β) is developed for structural mechanics problems based on the Hellinger-Reissner variational principle.According to the design principle of Pian, 15 unknown parameters are adopted in the selection of stress modes toavoid the zero energy modes.Meanwhile, the stress functions within each element satisfy both the equilibrium andthe compatibility relations of plane stress problems. Subsequently, numerical examples are presented to illustrate theeffectiveness and robustness of the proposed finite element. Numerical results show that various common lockingbehaviors of plane elements can be overcome. The PH-Q8-15β element has excellent performance in all benchmarkproblems, especially for structures with varying cross sections. Furthermore, in bending problems, the reasonablemesh shape of the new element for curved edge structures is analyzed in detail, which can be a useful means toimprove numerical accuracy. 展开更多
关键词 8-node plane hybrid element Hellinger-Reissner variational principle locking behaviors structural mechanics problems
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Review on dynamic analysis of road pavements under moving vehicles and plane strain conditions
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作者 Edmond V.Muho Niki D.Beskou 《Journal of Road Engineering》 2024年第1期54-68,共15页
This paper reviews works on the dynamic analysis of flexible and rigid pavements under moving vehicles on the basis of continuum-based plane strain models and linear theories.The purpose of this review is to provide i... This paper reviews works on the dynamic analysis of flexible and rigid pavements under moving vehicles on the basis of continuum-based plane strain models and linear theories.The purpose of this review is to provide in-formation about the existing works on the subject,critically discuss them and make suggestions for further research.The reviewed papers are presented on the basis of the various models for pavement-vehicle systems and the various methods for dynamically analyzing these systems.Flexible pavements are modeled by a homogeneous or layered half-plane with isotropic or anisotropic and linear elastic,viscoelastic or poroelastic material behavior.Rigid pavements are modeled by a beam or plate on a homogeneous or layered half-plane with material properties like the ones for flexible pavements.The vehicles are modeled as concentrated or distributed over a finite area loads moving with constant or time dependent speed.The above pavement-vehicle models are dynamically analyzed by analytical,analytical/numerical or purely numerical methods working in the time or frequency domain.Representative examples are presented to illustrate the models and methods of analysis,demonstrate their merits and assess the effects of the various parameters on pavement response.The paper closes with con-clusions and suggestions for further research in the area.The significance of this research effort has to do with the presentation of the existing literature on the subject in a critical and easy to understand way with the aid of representative examples and the identification of new research areas. 展开更多
关键词 Flexible pavements Rigid pavements Moving vehicles plane strain models Dynamic analysis
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Study on the Evolution of Plane Shape of Guangfu Ancestral Hall Buildings and the Construction of Type Genealogy in the Ming and Qing Dynasties
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作者 Yanming Chen Sharul Azim Sharudin Rui Chen 《Journal of Architectural Research and Development》 2024年第1期1-7,共7页
This paper studies the clan temples in four periods,namely,the development period(early Ming Dynasty and before),the shaping period(mid-Ming Dynasty to early Qing Dynasty),the maturity period(mid-Qing Dynasty),and the... This paper studies the clan temples in four periods,namely,the development period(early Ming Dynasty and before),the shaping period(mid-Ming Dynasty to early Qing Dynasty),the maturity period(mid-Qing Dynasty),and the programmed period(late Qing Dynasty),analyzes and summarizes their prototypical planform features and attempts to summarize and construct a genealogy of the architectural characteristics of the plane shape of clan temples.During the Ming and Qing dynasties,Guangfu ancestral halls in general showed a journey of development from a centripetal-single-linear-multi-linear-grid pattern,forming the unique Guangsanlu classic pattern in the Guangfu area. 展开更多
关键词 Guangfu clan Ancestral halls plane shape GENEALOGY
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(0001)面氧化锌单晶微纳米尺度划痕特性实验研究
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作者 李继军 李源明 +4 位作者 张丽华 郎风超 杨诗婷 王旭东 杨文欣 《表面技术》 EI CAS CSCD 北大核心 2023年第7期231-238,共8页
目的对(0001)面ZnO单晶微纳米尺度划痕特性进行实验研究,为ZnO单晶器件性能提升及ZnO单晶精密加工工艺优化等提供必要的科学依据。方法采用Berkovich金刚石压头棱向前和面向前2种划痕方式,在不同划痕速度下对(0001)面ZnO单晶进行了纳米... 目的对(0001)面ZnO单晶微纳米尺度划痕特性进行实验研究,为ZnO单晶器件性能提升及ZnO单晶精密加工工艺优化等提供必要的科学依据。方法采用Berkovich金刚石压头棱向前和面向前2种划痕方式,在不同划痕速度下对(0001)面ZnO单晶进行了纳米划痕实验,分析了划痕速度和划痕方式对其微纳米尺度划痕特性的影响。结果当划痕速度从2μm/s增加到100μm/s时,棱向前划痕方式下的深度从352.9 nm降到了326.9 nm,面向前划痕方式下的深度从352.7 nm降到了289.9 nm;棱向前划痕方式下的切向力从4.15 mN降到了3.93 mN,面向前划痕方式下的切向力从5.12 mN降到了4.45 mN;棱向前划痕方式下的摩擦因数从0.21降到了0.19,面向前划痕方式下的摩擦因数从0.25降到了0.2;棱向前划痕方式下的残余划痕深度从162.2 nm降到了138.4 nm,面向前划痕方式下的残余划痕深度从148.3 nm降到了129.9 nm;棱向前划痕方式下的残余划痕两侧塑性堆积高度从23 nm降到了17 nm,面向前划痕方式下的残余划痕两侧塑性堆积高度从18nm降到了11nm。结论随划痕速度的增加,(0001)面Zn O单晶的划痕深度、切向力、摩擦因数、残余划痕深度及划痕两侧塑性堆积高度均在下降。在相同划痕速度下,棱向前划痕方式下的划痕深度、残余划痕深度及划痕两侧塑性堆积高度都比面向前划痕方式下的要大,而棱向前划痕方式下的切向力和摩擦因数都比面向前划痕方式下的要小。 展开更多
关键词 (0001)面氧化锌单晶 微纳米尺度 划痕特性 纳米划痕 划痕速度 划痕方式
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《检测和校准实验室能力认可准则在微生物检测领域的 应用说明》(CNAS-CL01-A001)修订差异解析 被引量:2
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作者 宋义运 孙晓辰 +6 位作者 武维伟 张娜 万子晨 刘淑艳 徐昀 李宏 刘云国 《食品与发酵工业》 CAS CSCD 北大核心 2023年第1期347-353,共7页
通过对比《检测和校准实验室能力认可准则在微生物检测领域的应用说明》(CNAS-CL01-A001:202X)和《检测和校准实验室能力认可准则在微生物检测领域的应用说明》(CNAS-CL01-A001:2018)之间的差异,从新旧版本的变化情况和新版应用说明的... 通过对比《检测和校准实验室能力认可准则在微生物检测领域的应用说明》(CNAS-CL01-A001:202X)和《检测和校准实验室能力认可准则在微生物检测领域的应用说明》(CNAS-CL01-A001:2018)之间的差异,从新旧版本的变化情况和新版应用说明的关注点2个方面进行了解读。以对照表的形式详述新增、删除和变更内容,为申请认可实验室指明要点,帮助已通过认可实验室顺利完成转换。 展开更多
关键词 CNAS-CL01-A001 版本差异 关注点
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g-C_(3)N_(4)/{001}TiO_(2)异质结增强光催化剂降解MO
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作者 郝欣 田景芝 +2 位作者 赵云鹏 荆涛 齐海燕 《化学研究与应用》 CAS 北大核心 2023年第8期1854-1863,共10页
光催化技术因其反应条件温和而成为一种很有应用前景的污染物处理技术。本文采用热聚合法制备2D/2D石墨相氮化碳负载二氧化钛(g-C_(3)N_(4)/{001}TiO_(2))复合催化剂。通过XRD、FTIR、TEM、SEM、XPS和EIS等测试手段探究了g-C_(3)N_(4)和... 光催化技术因其反应条件温和而成为一种很有应用前景的污染物处理技术。本文采用热聚合法制备2D/2D石墨相氮化碳负载二氧化钛(g-C_(3)N_(4)/{001}TiO_(2))复合催化剂。通过XRD、FTIR、TEM、SEM、XPS和EIS等测试手段探究了g-C_(3)N_(4)和{001}TiO_(2)的微观形貌、结构、光生载流子的分离和迁移、催化机理以及之间异质结的成功构建。在氙灯模拟可见光下,对浓度为10 mg/L的甲基橙(MO)溶液进行降解测试,15-g-C_(3)N_(4)/{001}TiO_(2)的光催化活性最高。此外,研究表明含有不规则多孔状的g-C_(3)N_(4)/{001}TiO_(2)复合催化剂能有效的抑制光生电子-空穴对的复合,促进对太阳光的利用率,从而提高光催化活性。本研究为制备高效异质结光催化剂提供了新的方法。 展开更多
关键词 热聚合法 g-C_(3)N_(4) {001}TiO_(2) 光催化 水处理
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(001)/(101)晶面暴露TiO_(2)光催化氧化甘油制备甲酸研究
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作者 侯浩强 杨峥 +4 位作者 徐俪菲 马春慧 李伟 罗沙 刘守新 《功能材料》 CAS CSCD 北大核心 2023年第10期10207-10215,共9页
采用水热法制备了(001)/(101)晶面暴露的TiO_(2)催化剂,对其光催化氧化甘油制备甲酸性能进行了研究。利用SEM、TEM、XRD、XPS、EPR、Raman、UV-vis DRS和PL对TiO_(2)的结构和光学性能进行表征。结果表明,以钛酸异丙酯为Ti源,以氢氟酸为... 采用水热法制备了(001)/(101)晶面暴露的TiO_(2)催化剂,对其光催化氧化甘油制备甲酸性能进行了研究。利用SEM、TEM、XRD、XPS、EPR、Raman、UV-vis DRS和PL对TiO_(2)的结构和光学性能进行表征。结果表明,以钛酸异丙酯为Ti源,以氢氟酸为形貌控制剂,通过水热法合成的TiO_(2)暴露出(001)晶面。氢氟酸加入量为2 mL的样品(TiO_(2)-2F)同时暴露锐钛矿(001)和(101)晶面,表现出最高的催化活性和选择性。紫外光照射4 h,TiO_(2)-2F催化剂上甘油转化率为49.0%,甲酸选择性为55.6%。界面接触的(001)和(101)晶面形成表面异质结,抑制光生载流子的复合,提高甘油光催化氧化活性。(001)晶面的氧空位和配位不饱和O_(2c)-Ti_(5c)-O_(2c)键合方式使甘油及其中间产物深度氧化,提高甲酸选择性。机理分析表明,·OH和·O^(-)_(2)是甘油选择性氧化制备甲酸的关键活性物种。 展开更多
关键词 光催化 TiO_(2) (001)/(101)晶面 甘油 甲酸
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赤铁矿{001}和{012}晶面对Cr(Ⅵ)吸附差异性研究 被引量:1
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作者 廖荣 罗才贵 +2 位作者 高琦 钟丽娴 罗仙平 《有色金属科学与工程》 CAS 北大核心 2023年第6期862-870,共9页
赤铁矿是热稳定性最高的铁氧化物,其与环境污染物相互作用(吸附)对污染物的迁移、转化甚至环境归趋均具有重要影响。前人采用粗制赤铁矿研究其对污染物的吸附作用,所得结果难以准确说明其矿物表面的反应性质。基于此,采用水热法成功合... 赤铁矿是热稳定性最高的铁氧化物,其与环境污染物相互作用(吸附)对污染物的迁移、转化甚至环境归趋均具有重要影响。前人采用粗制赤铁矿研究其对污染物的吸附作用,所得结果难以准确说明其矿物表面的反应性质。基于此,采用水热法成功合成了主暴露{001}晶面、{012}晶面的赤铁矿纳米颗粒(HNPs和HNCs)。在此基础上,研究了赤铁矿不同晶面对Cr(Ⅵ)的吸附动力学和热力学特征,并考察了pH、离子强度等对赤铁矿不同晶面吸附Cr(Ⅵ)的影响。结果表明,HNPs与HNCs对Cr(Ⅵ)的吸附过程均遵循准二级动力学模型,且分别符合Langmuir和Freundlich等温吸附模型。随着温度升高,KL与KF均减小,△H^(ɵ)<0,说明吸附过程为放热过程。HNPs与HNCs对Cr(Ⅵ)的吸附量随溶液pH增大均减小。结合离子强度实验结果与DFT计算得出,HNPs对Cr(Ⅵ)的吸附主要以表面络合为主,推测吸附构型为单齿单核;而HNCs对Cr(Ⅵ)的吸附主要由表面络合与静电吸附共同主导,推测吸附构型为双齿双核。上述结果为赤铁矿应用于环境治理提供参考。 展开更多
关键词 赤铁矿 不同晶面 Cr(Ⅵ) 吸附构型
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Ni_(2)B(001)析氧反应性能的第一性原理
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作者 虞江涛 高洪涛 《青岛科技大学学报(自然科学版)》 CAS 2023年第6期57-62,共6页
采用基于第一性原理计算方法探讨了Ni_(2)B(001)晶面的电子结构和析氧反应(OER)性能。研究发现:Ni_(2)B(001)有着优异的电导率和OER活性。同周期过渡金属掺杂可有效地调节Ni_(2)B(001)电子特性并提高其OER活性,Cr掺杂效果尤其明显。电... 采用基于第一性原理计算方法探讨了Ni_(2)B(001)晶面的电子结构和析氧反应(OER)性能。研究发现:Ni_(2)B(001)有着优异的电导率和OER活性。同周期过渡金属掺杂可有效地调节Ni_(2)B(001)电子特性并提高其OER活性,Cr掺杂效果尤其明显。电荷密度和d带中心分析表明,掺杂原子M周围产生了更多的电子空穴,表面原子的d带中心更靠近费米能级,电子迁移率更高,OER催化活性更强。OER四电子反应过程中的第三步,即由中间体^(*)O至^(*)OOH的反应为电势决定步骤,当过电势为1.500 V时,OER反应自发进行。 展开更多
关键词 Ni_(2)B(001) 析氧反应 第一性原理
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UC001kfo通过靶向α-SMA对HeLa细胞增殖、迁移与侵袭的促进作用
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作者 任美颖 金燕 +1 位作者 樊思轩 舒丽莎 《中国老年学杂志》 CAS 北大核心 2023年第19期4781-4786,共6页
目的 探讨UC001kfo是否通过靶向α-平滑肌肌动蛋白(SMA)对宫颈癌HeLa细胞生物学行为产生影响。方法 采用RT-聚合酶链反应(PCR)技术检测UC001kfo在宫颈上皮永生化细胞株H8与宫颈癌细胞株HeLa中的表达;通过转染构建空白对照组、pCDNA3.1(+... 目的 探讨UC001kfo是否通过靶向α-平滑肌肌动蛋白(SMA)对宫颈癌HeLa细胞生物学行为产生影响。方法 采用RT-聚合酶链反应(PCR)技术检测UC001kfo在宫颈上皮永生化细胞株H8与宫颈癌细胞株HeLa中的表达;通过转染构建空白对照组、pCDNA3.1(+)-UC001kfo组、pCDNA3.1(+)-Vector组、siRNA-UC001kfo组和siRNA-NC组;分别利用CCK-8、划痕实验、Transwell检测细胞增殖、迁移与侵袭能力;运用RT-PCR技术检测α-SMA表达水平。结果 UC001kfo在Hela细胞中表达显著高于H8细胞(P<0.05);细胞功能实验结果显示上调UC001kfo表达能显著促进HeLa细胞增殖、迁移与侵袭能力;RT-PCR结果提示,与空白对照组和pCDNA3.1(+)-Vector组比较,pCDNA3.1(+)-UC001kfo组α-SMA mRNA及蛋白表达显著升高(P<0.05)。结论 UC001kfo在HeLa细胞中可能起促癌作用,其作用机制可能通过调节α-SMA产生对Hela细胞的影响,说明UC001kfo可能是宫颈癌靶向治疗的新型标记分子。 展开更多
关键词 宫颈癌 HELA细胞 UC001kfo 迁移 侵袭
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Crystal plane induced in-situ electrochemical activation of manganese-based cathode enable long-term aqueous zinc-ion batteries 被引量:1
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作者 Yuxin Gao Jiang Zhou +6 位作者 Liping Qin Zhenming Xu Zhexuan Liu Liangbing Wang Xinxin Cao Guozhao Fang Shuquan Liang 《Green Energy & Environment》 SCIE EI CAS CSCD 2023年第5期1429-1436,共8页
Rapid capacity decay and sluggish reaction kinetics are major barriers hindering the applications of manganese-based cathode materials for aqueous zinc-ion batteries.Herein,the effects of crystal plane on the in-situ ... Rapid capacity decay and sluggish reaction kinetics are major barriers hindering the applications of manganese-based cathode materials for aqueous zinc-ion batteries.Herein,the effects of crystal plane on the in-situ transformation behavior and electrochemical performance of manganese-based cathode is discussed.A comprehensive discussion manifests that the exposed(100)crystal plane is beneficial to the phase transformation from tunnel-structured MnO_(2) to layer-structured ZnMn_(3)O_(7)·3H_(2)O,which plays a critical role for the high reactivity,high capacity,fast diffusion kinetics and long cycling stability.Additionally,a two-stage zinc storage mechanism can be demonstrated,involving continuous activation reaction and phase transition reaction.As expected,it exhibits a high capacity of 275 mAh g^(-1)at 100 mA g^(-1),a superior durability over 1000 cycles and good rate capability.This study may open new windows toward developing advanced cathodes for ZIBs,and facilitate the applications of ZIBs in large-scale energy storage system. 展开更多
关键词 Crystal plane Electrochemical activation Phase transition reaction Cycling stability Zinc-ion batteries
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RRx-001对吉兰-巴雷综合征大鼠模型的保护作用及机制研究
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作者 高远 李煜 +4 位作者 武心语 杨慧 朱正杨 年娣 时鹏 《国际神经病学神经外科学杂志》 2023年第6期1-6,共6页
目的探讨RRx-001对实验性自身免疫性神经炎(EAN)的保护作用及机制的影响。方法采用人工合成P253~78肽段与完全弗氏佐剂混合免疫Lewis大鼠建立EAN模型,RRx-001腹腔注射给药。实验分为3组:对照组、模型组和实验组。通过检测大鼠体重和临... 目的探讨RRx-001对实验性自身免疫性神经炎(EAN)的保护作用及机制的影响。方法采用人工合成P253~78肽段与完全弗氏佐剂混合免疫Lewis大鼠建立EAN模型,RRx-001腹腔注射给药。实验分为3组:对照组、模型组和实验组。通过检测大鼠体重和临床评分,评估病情进展。采用透射电镜观察坐骨神经脱髓鞘变化。检测血液异硫氰酸荧光素―右旋糖酐,评估肠道屏障通透性。检测肠道一氧化氮(NO)、谷胱甘肽(GSH)和超氧化物歧化酶(SOD),评估氧化应激水平。采用ELISA试剂盒检测白细胞介素(IL)-1β、IL-18和肿瘤坏死因子α(TNF-α)表达情况。采用流式细胞术分析CD3^(+)T细胞、CD3^(+)CD4^(+)T细胞、CD3^(+)CD8^(+)T细胞、CD4^(+)CD44H^(+)T细胞、CD4^(+)CD62L^(+)T细胞和CD11b^(+)F4/80^(+)巨噬细胞。结果与模型组相比,RRx-001可以减少EAN大鼠体重丢失、降低临床评分(P<0.05);缓解坐骨神经脱髓鞘;提高SOD活力,降低IL-1β和TNF-α表达水平,减轻肠道损伤而改善肠通透性(P<0.001);降低CD4^(+)T/CD8^(+)T淋巴细胞和CD11b^(+)F4/80^(+)巨噬细胞比例,提升CD3^(+)T淋巴细胞的比例(P<0.05)。结论RRx-001可以改善EAN大鼠临床症状,其机制可能与调节免疫细胞活化和抑制炎症因子释放有关,发挥对EAN大鼠的保护作用。 展开更多
关键词 实验性自身免疫性神经炎 RRx-001 炎症因子 T淋巴细胞 巨噬细胞 大鼠
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ACT001通过STAT1/CIITA/MHC-Ⅱ通路发挥抗炎抗氧化活性治疗脓毒症引起的急性肺损伤
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作者 盛磊 周杰诗 +3 位作者 韩旭 李伊楠 刘慧娟 孙涛 《中国药理学通报》 CAS CSCD 北大核心 2023年第12期2231-2239,共9页
目的评价含笑内酯衍生物ACT001对脓毒症进程中急性肺损伤的治疗作用并探究其药理机制。方法动物水平上,小鼠腹腔注射LPS建立急性肺损伤模型,腹腔注射ACT001进行治疗,从小鼠个体存活状况、肺部炎症损伤及水肿情况等方面评价ACT001的药效... 目的评价含笑内酯衍生物ACT001对脓毒症进程中急性肺损伤的治疗作用并探究其药理机制。方法动物水平上,小鼠腹腔注射LPS建立急性肺损伤模型,腹腔注射ACT001进行治疗,从小鼠个体存活状况、肺部炎症损伤及水肿情况等方面评价ACT001的药效;细胞水平上,以LPS刺激RAW264.7细胞构建模型,通过检测炎症反应和氧化应激水平探究其药理机制,并通过蛋白质组学结果分析其相关分子机制。结果动物水平上,ACT001可改善急性肺损伤小鼠生存率、减轻肺部炎症、降低血清中炎症因子水平;细胞水平上,ACT001通过抑制MHC-Ⅱ相关通路,促进RAW264.7细胞向抗炎表型极化,抑制NO和相关炎症因子产生的同时提高SOD含量并清除ROS。结论ACT001通过抑制STAT1/CIITA/MHC-Ⅱ通路,发挥抗炎和抗氧化作用治疗急性肺损伤,有望开发为治疗脓毒症引起的急性肺损伤的新药。 展开更多
关键词 ACT001 LPS 脓毒症 急性肺损伤 MHC-Ⅱ 抗原处理与呈递
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Anti-Corrosion and Reconstruction of Surface Crystal Plane for Zn Anodes by an Advanced Metal Passivation Technique 被引量:1
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作者 Si Liu Hongxin Lin +2 位作者 Qianqian Song Jian Zhu Changbao Zhu 《Energy & Environmental Materials》 SCIE EI CAS CSCD 2023年第4期166-172,共7页
For the aqueous Zn-ion battery,dendrite formation,corrosion,and interfacial parasitic reactions are major issues,which greatly inhibits their practical application.How to develop a method of Zn construction or treatme... For the aqueous Zn-ion battery,dendrite formation,corrosion,and interfacial parasitic reactions are major issues,which greatly inhibits their practical application.How to develop a method of Zn construction or treatment to solve these issues for Zn anodes are still great challenges.Herein,a simple and cheap metal passivation technique is proposed for Zn anodes from a corrosion science perspective.Similar to the metal anticorrosion engineering,the formed interfacial protective layer in a chemical way can sufficiently solve the corrosion issues.Furthermore,the proposed passivity approach can reconstruct Zn surface-preferred crystal planes,exposing more(002)planes and improving surface hydrophilicity,which inhibits the formation of Zn dendrites and hydrogen evolution effectively.As expected,the passivated Zn achieves outstanding cycling life(1914 h)with low voltage polarization(<40 mV).Even at 6 mA cm^(−2) and 3 mA h cm^(−2),it can achieve stable Zn deposition over 460 h.The treated Zn anode coupled with MnO_(2) cathode shows prominently reinforced full batteries service life,making it a potential Zn anode candidate for excellent performance aqueous Zn-ion batteries.The proposed passivation approach provides a guideline for other metal electrodes preparation in various batteries and establishes the connections between corrosion science and batteries. 展开更多
关键词 ANTI-CORROSION aqueous zinc ion battery interfacial protective layer metal passivation technique reconstruction of surface crystal plane
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