Alkylation of diethyl 3 oxoglutarate monoanion by 4 bromobutene to produce enone diester 3, subsequent alkylation of 3 with 4 bromo 1 butene using K 2CO 3 and a catalytic amount of NaI in the mixed solvent afforded di...Alkylation of diethyl 3 oxoglutarate monoanion by 4 bromobutene to produce enone diester 3, subsequent alkylation of 3 with 4 bromo 1 butene using K 2CO 3 and a catalytic amount of NaI in the mixed solvent afforded dienone diester 4, which underwent decarboxylative hydrolysis when treated with 15% KOH in methanol under refluxing to provide dienone 5. The dienone afforded 2,8 dimethyl 1,7 dioxaspiroundecane 1 in good yield and high purity via an oxymercuration cyclisation reduction sequence.展开更多
Elevated activities of cytosolic fructose-1,6-bisphosphatase(cyFBPase) and sedoheptulose-1,7-bisphosphatase(SBPase)are associated with higher yields in plants. In this study, the expression levels of the cyFBPase and ...Elevated activities of cytosolic fructose-1,6-bisphosphatase(cyFBPase) and sedoheptulose-1,7-bisphosphatase(SBPase)are associated with higher yields in plants. In this study, the expression levels of the cyFBPase and SBPase genes were increased by overexpressing rape(Brassica napus) cDNA in tobacco(Nicotiana tabacum) plants. The transgenic plants coexpressing cy FBPase and SBPase(TpFS), or expressing single cy FBPase(TpF) or SBPase(TpS) had 1.77-, 1.55-, 1.23-fold cyFBPase and 1.45-, 1.12-, 1.36-fold SBPase activities as compared to the wild-type(WT), respectively. Photosynthesis rates of TpF, TpS and TpFS increased 4, 20 and 25% compared with WT plants. The SBPase and cyFBPase positively regulated each other and functioned synergistically in transgenic tobacco plants. In addition, the sucrose contents of the three transgenic plants were higher than that of WT plants. The starch accumulation of the TpFS and TpS plants was improved by 53 and 37%, but slightly decreased in TpF plants. Moreover, the transgenic tobacco plants harbouring SBPase and/or cyFBPase genes showed improvements in their growth, biomass, dry weight, plant height, stem diameter, leaf size,flower number, and pod weight. In conclusion, co-expression of SBPase and cyFBPase may pave a new way for improving crop yield in agricultural applications.展开更多
文摘Alkylation of diethyl 3 oxoglutarate monoanion by 4 bromobutene to produce enone diester 3, subsequent alkylation of 3 with 4 bromo 1 butene using K 2CO 3 and a catalytic amount of NaI in the mixed solvent afforded dienone diester 4, which underwent decarboxylative hydrolysis when treated with 15% KOH in methanol under refluxing to provide dienone 5. The dienone afforded 2,8 dimethyl 1,7 dioxaspiroundecane 1 in good yield and high purity via an oxymercuration cyclisation reduction sequence.
基金supported by the National Major Program on Transgenic Organisms from Ministry of Agriculture,China(2016ZX08005-004)。
文摘Elevated activities of cytosolic fructose-1,6-bisphosphatase(cyFBPase) and sedoheptulose-1,7-bisphosphatase(SBPase)are associated with higher yields in plants. In this study, the expression levels of the cyFBPase and SBPase genes were increased by overexpressing rape(Brassica napus) cDNA in tobacco(Nicotiana tabacum) plants. The transgenic plants coexpressing cy FBPase and SBPase(TpFS), or expressing single cy FBPase(TpF) or SBPase(TpS) had 1.77-, 1.55-, 1.23-fold cyFBPase and 1.45-, 1.12-, 1.36-fold SBPase activities as compared to the wild-type(WT), respectively. Photosynthesis rates of TpF, TpS and TpFS increased 4, 20 and 25% compared with WT plants. The SBPase and cyFBPase positively regulated each other and functioned synergistically in transgenic tobacco plants. In addition, the sucrose contents of the three transgenic plants were higher than that of WT plants. The starch accumulation of the TpFS and TpS plants was improved by 53 and 37%, but slightly decreased in TpF plants. Moreover, the transgenic tobacco plants harbouring SBPase and/or cyFBPase genes showed improvements in their growth, biomass, dry weight, plant height, stem diameter, leaf size,flower number, and pod weight. In conclusion, co-expression of SBPase and cyFBPase may pave a new way for improving crop yield in agricultural applications.
