1-Aminocyclopropane-1-carboxylic acid （ACC） synthase （ACS） is the principal enzyme in phytohormone ethylene biosynthesis. Previous studies have shown that the hypervariable C-terminus of ACS is proteolytically pro...1-Aminocyclopropane-1-carboxylic acid （ACC） synthase （ACS） is the principal enzyme in phytohormone ethylene biosynthesis. Previous studies have shown that the hypervariable C-terminus of ACS is proteolytically processed in vivo. However, the protease responsible for this has not yet been identified. In the present study, we investigated the processing of the 55-kDa full-length tomato ACS （LeACS2） into 52-, 50- and 49-kDa truncated isoforms in ripening tomato （Lycopersicon esculentum Mill. cv. Cooperation 903） fruit using the sodium dodecyl sulfate-boiling method. Meanwhile, an LeACS2-processing protease was purified via multi-step column chromatography from tomato fruit. Subsequent biochemical analysis of the 64-kDa purified protease revealed that it is a metalloprotease active at multiple cleavage sites within the hypervariable C-terminus of LeACS2. N-terminal sequencing and matrix-assisted laser desorption/ionization time-of-flight analysis indicated that the LeACS2-processing metalloprotease cleaves at the C-terminal sites Lys^438, Glu^447, Lys^448, Asn^456, Ser^460, Ser^462, Lys^463, and Leu^474, but does not cleave the N- terminus of LeACS2. Four C-terminus-deleted （26-50 amino acids） LeACS2 fusion proteins were overproduced and subjected to proteolysis by this metalloprotease to identify the multiple cleavage sites located on the N-terminal side of the phosphorylation site Ser^460. The results indisputably confirmed the presence of cleavage sites within the region between the α-helix domain （H14） and Ser^460 for this metalloprotease. Furthermore, the resulting C-terminally truncated LeACS2 isoforms were active enzymatically. Because this protease could produce LeACS2 isoforms in vitro similar to those detected in vivo, it is proposed that this metalloprotease may be involved in the proteolysis of LeACS2 in vivo.展开更多
目的探讨健脾疏肝丸对非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)大鼠肝X受体(liver X receptorα,LXRα)-固醇调节元件结合蛋白-1(sterolregulatory element-binding protein-1,SREBP-1)-脂肪酸合成酶(fatty acid s...目的探讨健脾疏肝丸对非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)大鼠肝X受体(liver X receptorα,LXRα)-固醇调节元件结合蛋白-1(sterolregulatory element-binding protein-1,SREBP-1)-脂肪酸合成酶(fatty acid synthase,FAS)信号转导通路的影响。方法将32只无特定病原体(specific pathogen free,SPF)SD雄性大鼠按照随机数字表法分为正常组、模型组、健脾疏肝丸组和易善复组,每组8只。正常组进食普通饲料,其他组进食高脂饲料,健脾疏肝丸组给予4.86 g/(kg·d)灌胃,易善复组给予0.123 g/(kg·d)灌胃,正常组和模型组给予等量蒸馏水灌胃。灌胃与造模同时进行,共8周。检测大鼠血清丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天门冬氨酸氨基转移酶(aspartate aminotransferase,AST)、高密度脂蛋白(high-density lipoprotein cholesterol,HDL-C)、低密度脂蛋白(low-density lipoprotein cholesterol,LDL-C)、甘油三酯(triglycerides,TG)、总胆固醇(total cholesterol,TC)、肿瘤坏死因子-α(tumor necrosis factor alpha,TNF-α)及白细胞介素-6(interleukin-6,IL-6)水平。对大鼠肝组织切片进行HE染色和油红O染色,于光学显微镜下观察。采用免疫组织化学法、蛋白质免疫印迹法及实时荧光定量聚合酶链式反应(real time polymerase chain reaction,RT-PCR)检测大鼠LXRα、SREBP-1及FAS的表达水平。结果 (1)正常组、模型组、健脾疏肝丸组和易善复组大鼠ALT[(3.40±0.81)U/L vs(9.98±2.27)U/L vs(7.80±1.52)U/L vs(6.43±1.89)U/L]、AST [(10.61±1.17)U/L vs(23.63±4.82)U/L vs(18.04±2.98)U/L vs(16.42±3.30)U/L]、TNF-α[(2.40±0.96)×10-3μg/L vs(6.64±0.92)×10-3μg/L vs(4.87±1.35)×10-3μg/L vs(4.45±1.39)×10-3μg/L]、IL-6 [(0.95±0.81)pg/ml vs(7.88±3.08)pg/ml vs(3.17±1.26)pg/ml vs(1.64±0.55)pg/ml]、TG [(0.33±0.13)mmol/L vs(0.90±0.24)mmol/L vs(0.62±0.37)mmol/L vs 0.62(0.46,0.66)mmol/L]、TC [(2.10±0.42)mmol/L vs 5.34(5.17,6.12)mmol/Lvs(3.68±0.63)mmol/Lvs(3.41±0.81)mmol/L]、HDL-C [(1.07±0.17)mmol/Lvs(0.62±0.14)mmol/Lvs(0.78±0.13)mmol/Lvs(0.79±0.12)mmol/L]及LDL-C[0.38(0.26,0.41)mmol/L vs(0.69±0.11)mmol/L vs(0.41±0.13)mmol/L vs(0.43±0.10)mmol/L]水平差异均有统计学意义(P <0.05)。与正常组相比,模型组AST、ALT、LDL-C、TG、TC、IL-6及TNF-α显著升高,HDL-C显著降低(P <0.05);与模型组相比,健脾疏肝丸组和易善复组AST、ALT、LDL-C、TG、TC、IL-6、TNF-α显著降低,HDL-C显著升高(P<0.05);与健脾疏肝丸组相比,易善复组大鼠血清TC显著降低(P <0.05),其他各指标差异无统计学意义(P> 0.05)。(2)肝组织HE染色和油红O染色示:与正常组相比,模型组大鼠肝脏脂肪变严重;与模型组相比,健脾疏肝丸组大鼠肝脏脂肪变减轻。(3)免疫组织化学结果表明,正常组、模型组、健脾疏肝丸组和易善复组大鼠LXRα(345872±52737 vs 544998±55506 vs 436319±65076 vs 448588±104641)、SREBP-1(259408±71143 vs 538701±62336vs 399705±102395 vs 394167±158047)和FAS(201683±48205 vs 466884±74934 vs 425589±63672 vs417852±84373)相对表达水平差异有统计学意义(P <0.05)。与正常组相比,模型组各蛋白相对表达水平均显著升高(P <0.05);与模型组相比,健脾疏肝丸组与易善复组各蛋白相对表达水平显著下降(P <0.05);健脾疏肝丸组与易善复组各蛋白相对表达水平差异无统计学意义(P> 0.05)。(4)Western blot结果表明,正常组、模型组、健脾疏肝丸组和易善复组大鼠LXRα[0.80±0.29 vs 1.57(1.30,1.67) vs 1.09±0.30 vs 1.10±0.36]、SREBP-1(0.42±0.12 vs 1.15±0.45 vs 0.86±0.20 vs 0.84±0.20)和FAS(0.43±0.12 vs 1.10±0.40 vs 0.81±0.26 vs 0.80±0.28)相对表达水平差异有统计学意义(P <0.05)。与正常组对比,模型组小鼠各蛋白相对表达水平显著升高(P <0.05);与模型组对比,健脾疏肝丸组及易善复组小鼠LXRα蛋白相对表达水平显著降低(P <0.05),健脾疏肝丸组与易善复组相比,各蛋白相对表达水平差异无统计学意义(P> 0.05)。(5)正常组、模型组、健脾疏肝丸组和易善复组大鼠肝组织LXRα[1.13±0.38 vs 4.14(4.01,4.35) vs 2.65±1.85 vs 1.35(0.54,4.23)]、SREBP-1、FAS[1.37±0.49 vs 4.35±1.97 vs 1.98(1.88,3.22)m RNA相对表达量差异有统计学意义(P <0.05)。与正常组相比,模型组LXRα[1.46±0.51 vs 6.13±1.17 vs 3.82±2.06 vs 1.56(1.19,4.74)]、SREBP-1、FAS vs1.83(1.64,4.29)] m RNA相对表达量显著升高(P <0.05);与模型组对比,健脾疏肝丸组及易善复组LXRα、SREBP-1、FAS m RNA表达均显著降低(P <0.05),健脾疏肝丸组与易善复组相比,LXRα、SREBP-1、FAS m RNA表达差异无统计学意义(P> 0.05)。结论健脾疏肝丸对大鼠NAFLD的改善作用可能与其抑制LXRα-SREBP-1-FAS信号转导通路有关。展开更多
Tomato （Lycopslcon esculentum Mill.） plants grown in a greenhouse were irradiated with two different levels of UV-B, namely 8.82 （T1） and 12.6 kJ/m^2 per day （T2）. Ethylene production, 1-aminocyclopropane-1-carb...Tomato （Lycopslcon esculentum Mill.） plants grown in a greenhouse were irradiated with two different levels of UV-B, namely 8.82 （T1） and 12.6 kJ/m^2 per day （T2）. Ethylene production, 1-aminocyclopropane-1-carboxylate （ACC） content, 1-（malonylamino） cyclopvopane-1-carboxylic acid （MACC） content, gene expression of ACC aynthase （EC 4.4.1.14）, and ACC oxidase activity in tomato leaves were determined. The results Indicated that ACC content, the activity of ACC synthase and ACC oxidase, and ethylene production Increased continuously under low doses of UV-B radiation, whereas at high doses of radiation these parameters Increased during the first 12 d and then started to decrease. The MACC content increased continuously over 18 d under both doses of UV-B irradiation. The changes in ACC content, ACC synthaae activity, ACC oxidase activity, the transcriptional level of the ACC synthase gene, and ethylene production were consistent with each other, suggesting that ACC synthase was the key enzyme in ethylene biosynthesis and that ethylene production in tomato leaf tissues under UV-B radiation could be regulated by the expression of the ACC synthase gene. The results also indicate that the change in ethylene metabolism may be an adaptive mechanism to enhanced UV-B radiation.展开更多
OBJECTIVE: To study the mechanism of Dangfei Liganning capsule(当飞利肝宁胶囊) in the treatment of rats with metabolic associated fatty liver disease(MAFLD). METHODS: Totally 48 specific pathogen free SpragueDawley ma...OBJECTIVE: To study the mechanism of Dangfei Liganning capsule(当飞利肝宁胶囊) in the treatment of rats with metabolic associated fatty liver disease(MAFLD). METHODS: Totally 48 specific pathogen free SpragueDawley male rats were randomly divided into normal Group, model group, Dangfei Liganning high, moderate, and low-dose groups and Essentiale group which were fed with high fat diet for 8 weeks, and gavage and molding were carried out simultaneously. Dangfei Liganning high, middle and low-dose group were given 0.27, 0.135 and 0.0675 g·kg-1·d-1 respectively by gavage, Essentiale group was given 0.123 g·kg-1·d-1 by gavage, the same amount of distilled water was given by gavage in the normal group and the model group. The rats were weighed at the 0th week, 2nd week, 4th week, 6th week and 8th weekend respectively. The rats were sacrificed at the end of the 8th week. Serum levels of alanine aminotransferase(ALT), alanine aminotransferase(AST),triglyceride(TG), total cholesterol(CHO), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein (LDL-C), total protein(TP), albumin(Alb), globulin(GLB), total bilirubin(TBIL), direct bilirubin(DBIL), tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) were measured. The levels of liver tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) and liver pathology [hematoxylin and eosin(HE) staining, oil red O staining] were detected. The expression levels of liver X receptor α(LXRα), steroid regulatory element binding protein-1(SREBP-1) and fatty acid synthase(FAS) were detected by immunohistochemistry, Western blot and reverse transcription-polymerase chain reaction reverse transcription-polymerase chain reaction. RESULTS: From the beginning to the 8th week, the growth rate of body weight in the Dangfei Liganning highdose group was slower than all other groups. There was no significant difference in ALB level in all groups(P > 0.05). Compared with the model group, the levels of ALT, AST, LDL-C, TG, CHO, TP, GLB, TBIL, DBIL, IL-6, TNF-α were significantly decreased and HDL-C were significantly increased in Dangfei Liganning high-dose group(P < 0.01, < 0.05). HE and oil red O staining showed that the fatty lesions in rat liver were alleviated, while the expressions of LXRα, SREBP-1, FAS m RNA and protein were significantly decreased(P < 0.01). CONCLUSIONS: Dangfei Liganning capsule can slow down the increase of body weight of MAFLD rats, reduce the levels of transaminase, Lipid and inflammatory factors in MAFLD rats, promote the synthesis of liver protein and bile metabolism, and improve the liver fatty lesion of MAFLD rats, among which the Dangfei Liganning highdose group is more effective. The mechanism of action may be through blocking LXR-SREBP-1-FAS signal pathway.展开更多
基金Supported by Hong Kong Research Grant Council （HKUST6102/02M and HKUST6105/01M） and the National Natural Science Foundation of China （30129001）.
文摘1-Aminocyclopropane-1-carboxylic acid （ACC） synthase （ACS） is the principal enzyme in phytohormone ethylene biosynthesis. Previous studies have shown that the hypervariable C-terminus of ACS is proteolytically processed in vivo. However, the protease responsible for this has not yet been identified. In the present study, we investigated the processing of the 55-kDa full-length tomato ACS （LeACS2） into 52-, 50- and 49-kDa truncated isoforms in ripening tomato （Lycopersicon esculentum Mill. cv. Cooperation 903） fruit using the sodium dodecyl sulfate-boiling method. Meanwhile, an LeACS2-processing protease was purified via multi-step column chromatography from tomato fruit. Subsequent biochemical analysis of the 64-kDa purified protease revealed that it is a metalloprotease active at multiple cleavage sites within the hypervariable C-terminus of LeACS2. N-terminal sequencing and matrix-assisted laser desorption/ionization time-of-flight analysis indicated that the LeACS2-processing metalloprotease cleaves at the C-terminal sites Lys^438, Glu^447, Lys^448, Asn^456, Ser^460, Ser^462, Lys^463, and Leu^474, but does not cleave the N- terminus of LeACS2. Four C-terminus-deleted （26-50 amino acids） LeACS2 fusion proteins were overproduced and subjected to proteolysis by this metalloprotease to identify the multiple cleavage sites located on the N-terminal side of the phosphorylation site Ser^460. The results indisputably confirmed the presence of cleavage sites within the region between the α-helix domain （H14） and Ser^460 for this metalloprotease. Furthermore, the resulting C-terminally truncated LeACS2 isoforms were active enzymatically. Because this protease could produce LeACS2 isoforms in vitro similar to those detected in vivo, it is proposed that this metalloprotease may be involved in the proteolysis of LeACS2 in vivo.
文摘目的探讨健脾疏肝丸对非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)大鼠肝X受体(liver X receptorα,LXRα)-固醇调节元件结合蛋白-1(sterolregulatory element-binding protein-1,SREBP-1)-脂肪酸合成酶(fatty acid synthase,FAS)信号转导通路的影响。方法将32只无特定病原体(specific pathogen free,SPF)SD雄性大鼠按照随机数字表法分为正常组、模型组、健脾疏肝丸组和易善复组,每组8只。正常组进食普通饲料,其他组进食高脂饲料,健脾疏肝丸组给予4.86 g/(kg·d)灌胃,易善复组给予0.123 g/(kg·d)灌胃,正常组和模型组给予等量蒸馏水灌胃。灌胃与造模同时进行,共8周。检测大鼠血清丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天门冬氨酸氨基转移酶(aspartate aminotransferase,AST)、高密度脂蛋白(high-density lipoprotein cholesterol,HDL-C)、低密度脂蛋白(low-density lipoprotein cholesterol,LDL-C)、甘油三酯(triglycerides,TG)、总胆固醇(total cholesterol,TC)、肿瘤坏死因子-α(tumor necrosis factor alpha,TNF-α)及白细胞介素-6(interleukin-6,IL-6)水平。对大鼠肝组织切片进行HE染色和油红O染色,于光学显微镜下观察。采用免疫组织化学法、蛋白质免疫印迹法及实时荧光定量聚合酶链式反应(real time polymerase chain reaction,RT-PCR)检测大鼠LXRα、SREBP-1及FAS的表达水平。结果 (1)正常组、模型组、健脾疏肝丸组和易善复组大鼠ALT[(3.40±0.81)U/L vs(9.98±2.27)U/L vs(7.80±1.52)U/L vs(6.43±1.89)U/L]、AST [(10.61±1.17)U/L vs(23.63±4.82)U/L vs(18.04±2.98)U/L vs(16.42±3.30)U/L]、TNF-α[(2.40±0.96)×10-3μg/L vs(6.64±0.92)×10-3μg/L vs(4.87±1.35)×10-3μg/L vs(4.45±1.39)×10-3μg/L]、IL-6 [(0.95±0.81)pg/ml vs(7.88±3.08)pg/ml vs(3.17±1.26)pg/ml vs(1.64±0.55)pg/ml]、TG [(0.33±0.13)mmol/L vs(0.90±0.24)mmol/L vs(0.62±0.37)mmol/L vs 0.62(0.46,0.66)mmol/L]、TC [(2.10±0.42)mmol/L vs 5.34(5.17,6.12)mmol/Lvs(3.68±0.63)mmol/Lvs(3.41±0.81)mmol/L]、HDL-C [(1.07±0.17)mmol/Lvs(0.62±0.14)mmol/Lvs(0.78±0.13)mmol/Lvs(0.79±0.12)mmol/L]及LDL-C[0.38(0.26,0.41)mmol/L vs(0.69±0.11)mmol/L vs(0.41±0.13)mmol/L vs(0.43±0.10)mmol/L]水平差异均有统计学意义(P <0.05)。与正常组相比,模型组AST、ALT、LDL-C、TG、TC、IL-6及TNF-α显著升高,HDL-C显著降低(P <0.05);与模型组相比,健脾疏肝丸组和易善复组AST、ALT、LDL-C、TG、TC、IL-6、TNF-α显著降低,HDL-C显著升高(P<0.05);与健脾疏肝丸组相比,易善复组大鼠血清TC显著降低(P <0.05),其他各指标差异无统计学意义(P> 0.05)。(2)肝组织HE染色和油红O染色示:与正常组相比,模型组大鼠肝脏脂肪变严重;与模型组相比,健脾疏肝丸组大鼠肝脏脂肪变减轻。(3)免疫组织化学结果表明,正常组、模型组、健脾疏肝丸组和易善复组大鼠LXRα(345872±52737 vs 544998±55506 vs 436319±65076 vs 448588±104641)、SREBP-1(259408±71143 vs 538701±62336vs 399705±102395 vs 394167±158047)和FAS(201683±48205 vs 466884±74934 vs 425589±63672 vs417852±84373)相对表达水平差异有统计学意义(P <0.05)。与正常组相比,模型组各蛋白相对表达水平均显著升高(P <0.05);与模型组相比,健脾疏肝丸组与易善复组各蛋白相对表达水平显著下降(P <0.05);健脾疏肝丸组与易善复组各蛋白相对表达水平差异无统计学意义(P> 0.05)。(4)Western blot结果表明,正常组、模型组、健脾疏肝丸组和易善复组大鼠LXRα[0.80±0.29 vs 1.57(1.30,1.67) vs 1.09±0.30 vs 1.10±0.36]、SREBP-1(0.42±0.12 vs 1.15±0.45 vs 0.86±0.20 vs 0.84±0.20)和FAS(0.43±0.12 vs 1.10±0.40 vs 0.81±0.26 vs 0.80±0.28)相对表达水平差异有统计学意义(P <0.05)。与正常组对比,模型组小鼠各蛋白相对表达水平显著升高(P <0.05);与模型组对比,健脾疏肝丸组及易善复组小鼠LXRα蛋白相对表达水平显著降低(P <0.05),健脾疏肝丸组与易善复组相比,各蛋白相对表达水平差异无统计学意义(P> 0.05)。(5)正常组、模型组、健脾疏肝丸组和易善复组大鼠肝组织LXRα[1.13±0.38 vs 4.14(4.01,4.35) vs 2.65±1.85 vs 1.35(0.54,4.23)]、SREBP-1、FAS[1.37±0.49 vs 4.35±1.97 vs 1.98(1.88,3.22)m RNA相对表达量差异有统计学意义(P <0.05)。与正常组相比,模型组LXRα[1.46±0.51 vs 6.13±1.17 vs 3.82±2.06 vs 1.56(1.19,4.74)]、SREBP-1、FAS vs1.83(1.64,4.29)] m RNA相对表达量显著升高(P <0.05);与模型组对比,健脾疏肝丸组及易善复组LXRα、SREBP-1、FAS m RNA表达均显著降低(P <0.05),健脾疏肝丸组与易善复组相比,LXRα、SREBP-1、FAS m RNA表达差异无统计学意义(P> 0.05)。结论健脾疏肝丸对大鼠NAFLD的改善作用可能与其抑制LXRα-SREBP-1-FAS信号转导通路有关。
基金Supported by the National Natural Science Foundation of China （30170038, 90302010）, ＂Hundred talents＂ project of the Chinese Academy of Sciences, Gansu Key Technologies R＆D Program （GS022-A41-045）, and Gansu Agricultural Bio-technology Research ＆ Development Project. Acknowledgements The authors thank Dr Akira Nakatsuka （Laboratory of Postharvest Agriculture, Faculty of Agriculture, 0kayama University, 0kayama, Japan） for providing the ACC synthase clone.
文摘Tomato （Lycopslcon esculentum Mill.） plants grown in a greenhouse were irradiated with two different levels of UV-B, namely 8.82 （T1） and 12.6 kJ/m^2 per day （T2）. Ethylene production, 1-aminocyclopropane-1-carboxylate （ACC） content, 1-（malonylamino） cyclopvopane-1-carboxylic acid （MACC） content, gene expression of ACC aynthase （EC 4.4.1.14）, and ACC oxidase activity in tomato leaves were determined. The results Indicated that ACC content, the activity of ACC synthase and ACC oxidase, and ethylene production Increased continuously under low doses of UV-B radiation, whereas at high doses of radiation these parameters Increased during the first 12 d and then started to decrease. The MACC content increased continuously over 18 d under both doses of UV-B irradiation. The changes in ACC content, ACC synthaae activity, ACC oxidase activity, the transcriptional level of the ACC synthase gene, and ethylene production were consistent with each other, suggesting that ACC synthase was the key enzyme in ethylene biosynthesis and that ethylene production in tomato leaf tissues under UV-B radiation could be regulated by the expression of the ACC synthase gene. The results also indicate that the change in ethylene metabolism may be an adaptive mechanism to enhanced UV-B radiation.
基金Supported by Capital Health Development Research Project:Assessment of the Efficacy of BIEJIAJIANWAN Pill in Patients with Chronic Hepatitis B Cirrhosis/Fibrosis (CD2018-2-2173)Beijing Municipal Administration of Hospitals Incubating Program:Clinical Observation on the Treatment of Nonalcoholic Fatty Liver Disease by Invigorating the Spleen,Soothing the Liver,Activating Blood Circulation and Resolving Phlegm (PZ2019011)。
文摘OBJECTIVE: To study the mechanism of Dangfei Liganning capsule(当飞利肝宁胶囊) in the treatment of rats with metabolic associated fatty liver disease(MAFLD). METHODS: Totally 48 specific pathogen free SpragueDawley male rats were randomly divided into normal Group, model group, Dangfei Liganning high, moderate, and low-dose groups and Essentiale group which were fed with high fat diet for 8 weeks, and gavage and molding were carried out simultaneously. Dangfei Liganning high, middle and low-dose group were given 0.27, 0.135 and 0.0675 g·kg-1·d-1 respectively by gavage, Essentiale group was given 0.123 g·kg-1·d-1 by gavage, the same amount of distilled water was given by gavage in the normal group and the model group. The rats were weighed at the 0th week, 2nd week, 4th week, 6th week and 8th weekend respectively. The rats were sacrificed at the end of the 8th week. Serum levels of alanine aminotransferase(ALT), alanine aminotransferase(AST),triglyceride(TG), total cholesterol(CHO), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein (LDL-C), total protein(TP), albumin(Alb), globulin(GLB), total bilirubin(TBIL), direct bilirubin(DBIL), tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) were measured. The levels of liver tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) and liver pathology [hematoxylin and eosin(HE) staining, oil red O staining] were detected. The expression levels of liver X receptor α(LXRα), steroid regulatory element binding protein-1(SREBP-1) and fatty acid synthase(FAS) were detected by immunohistochemistry, Western blot and reverse transcription-polymerase chain reaction reverse transcription-polymerase chain reaction. RESULTS: From the beginning to the 8th week, the growth rate of body weight in the Dangfei Liganning highdose group was slower than all other groups. There was no significant difference in ALB level in all groups(P > 0.05). Compared with the model group, the levels of ALT, AST, LDL-C, TG, CHO, TP, GLB, TBIL, DBIL, IL-6, TNF-α were significantly decreased and HDL-C were significantly increased in Dangfei Liganning high-dose group(P < 0.01, < 0.05). HE and oil red O staining showed that the fatty lesions in rat liver were alleviated, while the expressions of LXRα, SREBP-1, FAS m RNA and protein were significantly decreased(P < 0.01). CONCLUSIONS: Dangfei Liganning capsule can slow down the increase of body weight of MAFLD rats, reduce the levels of transaminase, Lipid and inflammatory factors in MAFLD rats, promote the synthesis of liver protein and bile metabolism, and improve the liver fatty lesion of MAFLD rats, among which the Dangfei Liganning highdose group is more effective. The mechanism of action may be through blocking LXR-SREBP-1-FAS signal pathway.
