A β-cyclodextrin derivative, perbenzyl-β-cyclodextrin, was prepared and used as a chiral stationary phase for capillary gas chromatography. Using FID detector and the column temperature between 70 and 80℃, the chir...A β-cyclodextrin derivative, perbenzyl-β-cyclodextrin, was prepared and used as a chiral stationary phase for capillary gas chromatography. Using FID detector and the column temperature between 70 and 80℃, the chiral separations of racemic 3-phenyl-1, 2-epoxyethane and 1-phenyl-1-propanol on a 14m×0.23mm I. D. fused silica column with the β-cyclodextrin derivative were carried out and the optical purities of optically active 1-phenyl-1-propanol samples prepared by asymmetric synthesis were determined.展开更多
The title compound trans-4-[（5-（2,4-dichlorophenoxy）-3-methyl- 1-phenyl-1H-pyrazol-4-yl）methyleneamino]- 1,5-dimethyl-2-phenyl-1,2-dihydropyrazol-3-one 3 （C28H23Cl2N5O2, Mr = 532.41） has been synthesized and its...The title compound trans-4-[（5-（2,4-dichlorophenoxy）-3-methyl- 1-phenyl-1H-pyrazol-4-yl）methyleneamino]- 1,5-dimethyl-2-phenyl-1,2-dihydropyrazol-3-one 3 （C28H23Cl2N5O2, Mr = 532.41） has been synthesized and its crystal structure was determined by single-crystal X-ray diffraction analysis. It crystallizes in triclinic, space group P1- with a = 8.9438（4）, b = 11.6065（5）, c = 14.2215（6）A, α = 112.566（1）, β = 92.324（2）, γ = 102.91（1）°, V= 1315.65（10） A^3, Z = 2, Dc = 1.344 g/cm^3,μ（MoKa） = 0.282 mm^-1, λ = 0.71073 A, F（000） = 552, the final R = 0.0587 and wR = 0.1578 for 5071 observed reflections （I 〉 2σ（I））. X-ray analysis reveals that the product is a thermodynamically stable trans isomer. Intra- and intermolecular C（ 12）-H（12）…O（1） and C（28）-H（28）...O（1）# 1 hydrogen bonds were observed in the title compound.展开更多
The synthesis of(S)-1-phenyl-1,2-ethanediol was studied in this article.Firstly,1,2-dibromo-1-phenylethane was synthesized with styrene and bromine as starting agents.The effects of some factors,including raw material...The synthesis of(S)-1-phenyl-1,2-ethanediol was studied in this article.Firstly,1,2-dibromo-1-phenylethane was synthesized with styrene and bromine as starting agents.The effects of some factors,including raw materials’ molar ratio,reaction time and solvents,on the yield of 1,2-dibromo-1-phenylethane were examined.Secondly,1-phenyl-1,2-ethanediol was obtained by use of hydrolysis of 1,2-dibromo-1-phenylethane in alkaline medium.The influence of reaction time and medium on the yield of 1-phenyl-1,2-ethanediol was also examined.Under the optimized conditions,the yield of 1,2-dibromo-1-phenylethane was 90.1% while the yield of 1-phenyl-1,2-ethanediol was 78.5%.Finally,(S)-1-phenyl-1,2-ethanediol was received by deracemization of 1-phenyl-1,2-ethanediol employing Candida parapsilosis.The enantiomeric excess of(S)-1-phenyl-1,2-ethanediol was 99% and the conversion was 88%.展开更多
The gene which encodes(R)-specific carbonyl reductase(rCR)from Candida parapsilosis CCTCC M203011 was cloned,sequenced and compared with genes from the GenBank.The results indicated that rCR gene was 1011 bp,encoding ...The gene which encodes(R)-specific carbonyl reductase(rCR)from Candida parapsilosis CCTCC M203011 was cloned,sequenced and compared with genes from the GenBank.The results indicated that rCR gene was 1011 bp,encoding a protein of 336 amino acids with a molecular weight of 35.9 kDa,and its nucleotide sequence showed 99%similarity to those of other members of the alcohol dehydrogenase superfamily.The rCR gene could express in recombinant strain Escherichia coli JM109,and the expression plasmid could produce(R)-1-pheny-1,2-ethanediol(100%e.e.,80.14%yield)from b-hydroxyacetophenone without any additive to regenerate NAD+from NADH.展开更多
The present study observed the action of 1H-indole-2, 3-dione （isatin） on Bax protein expression in the substantia nigra of a Parkinson＇s disease animal model. Parkinson＇s disease-like behaviors were induced in C5...The present study observed the action of 1H-indole-2, 3-dione （isatin） on Bax protein expression in the substantia nigra of a Parkinson＇s disease animal model. Parkinson＇s disease-like behaviors were induced in C57BL/6J mice treated with 1-methyl-4-phenyl-1,2, 3, 6-tetrahydropyridine （MPTP） Bax protein expression was significantly reduced in isatin （100, 200 mg/kg）-pretreated mice. Results demonstrate that isatin plays a neuroprotective role in mice treated with MPTP by down-regulating Bax protein expression.展开更多
A novel NADPH-dependent carbonyl reductase was separated from Candida parapsilosis CCTCC 203011.The enzyme gave a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE),which was purified t...A novel NADPH-dependent carbonyl reductase was separated from Candida parapsilosis CCTCC 203011.The enzyme gave a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE),which was purified through ammonium sulfate,Diethylamino Ethanol(DEAE)sepharose Fast flow(FF),phenyl-sepharose FF and blue sepharose FF chromato graphy from cell-free extract.The molecular mass of the enzyme was about 30 kDa.The optimum pH and temperature for reduction were 4.5℃ and 35℃,respectively.The Cu2+had strong restrictive effect on enzyme activity.In addition,the carbonyl reductase was an enzyme with high substrate specificity and stereo-selectivity,and showed high asymmetric reduction activity towards a-hydroxyacetophenone and ethyl 4-chloro acetoacetate.For the asymmetric reduction of a-hydroxyacetophenone and ethyl 4-chloro acetoacetate,(S)-1-phenyl-1,2-ethanediol and(R)-ethyl 4-chloro-3-hydroxybutanoate were produced by the purified enzyme,with the 100% and 94.3%e.e.value,respec-tively.Therefore,the enzyme could be one of the effective biocatalysts for asymmetric synthesis of chiral alcohols.The amino acid sequences of one peptide from the purified enzyme were analyzed by LC-MASS-MASS,and the car-bonyl reductase showed some identity to the hypothetical protein CaO19.10414 reported.展开更多
文摘A β-cyclodextrin derivative, perbenzyl-β-cyclodextrin, was prepared and used as a chiral stationary phase for capillary gas chromatography. Using FID detector and the column temperature between 70 and 80℃, the chiral separations of racemic 3-phenyl-1, 2-epoxyethane and 1-phenyl-1-propanol on a 14m×0.23mm I. D. fused silica column with the β-cyclodextrin derivative were carried out and the optical purities of optically active 1-phenyl-1-propanol samples prepared by asymmetric synthesis were determined.
基金the Science Research Foundation of Henan Institute of Science and Technology (No. 06036)
文摘The title compound trans-4-[（5-（2,4-dichlorophenoxy）-3-methyl- 1-phenyl-1H-pyrazol-4-yl）methyleneamino]- 1,5-dimethyl-2-phenyl-1,2-dihydropyrazol-3-one 3 （C28H23Cl2N5O2, Mr = 532.41） has been synthesized and its crystal structure was determined by single-crystal X-ray diffraction analysis. It crystallizes in triclinic, space group P1- with a = 8.9438（4）, b = 11.6065（5）, c = 14.2215（6）A, α = 112.566（1）, β = 92.324（2）, γ = 102.91（1）°, V= 1315.65（10） A^3, Z = 2, Dc = 1.344 g/cm^3,μ（MoKa） = 0.282 mm^-1, λ = 0.71073 A, F（000） = 552, the final R = 0.0587 and wR = 0.1578 for 5071 observed reflections （I 〉 2σ（I））. X-ray analysis reveals that the product is a thermodynamically stable trans isomer. Intra- and intermolecular C（ 12）-H（12）…O（1） and C（28）-H（28）...O（1）# 1 hydrogen bonds were observed in the title compound.
文摘The synthesis of(S)-1-phenyl-1,2-ethanediol was studied in this article.Firstly,1,2-dibromo-1-phenylethane was synthesized with styrene and bromine as starting agents.The effects of some factors,including raw materials’ molar ratio,reaction time and solvents,on the yield of 1,2-dibromo-1-phenylethane were examined.Secondly,1-phenyl-1,2-ethanediol was obtained by use of hydrolysis of 1,2-dibromo-1-phenylethane in alkaline medium.The influence of reaction time and medium on the yield of 1-phenyl-1,2-ethanediol was also examined.Under the optimized conditions,the yield of 1,2-dibromo-1-phenylethane was 90.1% while the yield of 1-phenyl-1,2-ethanediol was 78.5%.Finally,(S)-1-phenyl-1,2-ethanediol was received by deracemization of 1-phenyl-1,2-ethanediol employing Candida parapsilosis.The enantiomeric excess of(S)-1-phenyl-1,2-ethanediol was 99% and the conversion was 88%.
基金This study was supported by the National Basic Research Program of China(No.2003CB716008)the Program for Changjiang Scholars and Innovative Research Team in University(PCSIRT,IRT0532).
文摘The gene which encodes(R)-specific carbonyl reductase(rCR)from Candida parapsilosis CCTCC M203011 was cloned,sequenced and compared with genes from the GenBank.The results indicated that rCR gene was 1011 bp,encoding a protein of 336 amino acids with a molecular weight of 35.9 kDa,and its nucleotide sequence showed 99%similarity to those of other members of the alcohol dehydrogenase superfamily.The rCR gene could express in recombinant strain Escherichia coli JM109,and the expression plasmid could produce(R)-1-pheny-1,2-ethanediol(100%e.e.,80.14%yield)from b-hydroxyacetophenone without any additive to regenerate NAD+from NADH.
基金a grant from Shandong Provincial Education Department, No. J08LH54
文摘The present study observed the action of 1H-indole-2, 3-dione （isatin） on Bax protein expression in the substantia nigra of a Parkinson＇s disease animal model. Parkinson＇s disease-like behaviors were induced in C57BL/6J mice treated with 1-methyl-4-phenyl-1,2, 3, 6-tetrahydropyridine （MPTP） Bax protein expression was significantly reduced in isatin （100, 200 mg/kg）-pretreated mice. Results demonstrate that isatin plays a neuroprotective role in mice treated with MPTP by down-regulating Bax protein expression.
基金This work was supported by the National Natural Science Foundation of China(Grant No.20376031)the National Key Basic Research and Development Program of China(973 Program)(Grant No.2003CB716008)+1 种基金the Program for New Century Excellent Talents in University,Ministry of Education,China(Grant No.NCET-04-0498)the Program for Changjiang Scholars and Innovative Research Team in University(Grant No.PCSIRT,IRT0532).
文摘A novel NADPH-dependent carbonyl reductase was separated from Candida parapsilosis CCTCC 203011.The enzyme gave a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE),which was purified through ammonium sulfate,Diethylamino Ethanol(DEAE)sepharose Fast flow(FF),phenyl-sepharose FF and blue sepharose FF chromato graphy from cell-free extract.The molecular mass of the enzyme was about 30 kDa.The optimum pH and temperature for reduction were 4.5℃ and 35℃,respectively.The Cu2+had strong restrictive effect on enzyme activity.In addition,the carbonyl reductase was an enzyme with high substrate specificity and stereo-selectivity,and showed high asymmetric reduction activity towards a-hydroxyacetophenone and ethyl 4-chloro acetoacetate.For the asymmetric reduction of a-hydroxyacetophenone and ethyl 4-chloro acetoacetate,(S)-1-phenyl-1,2-ethanediol and(R)-ethyl 4-chloro-3-hydroxybutanoate were produced by the purified enzyme,with the 100% and 94.3%e.e.value,respec-tively.Therefore,the enzyme could be one of the effective biocatalysts for asymmetric synthesis of chiral alcohols.The amino acid sequences of one peptide from the purified enzyme were analyzed by LC-MASS-MASS,and the car-bonyl reductase showed some identity to the hypothetical protein CaO19.10414 reported.
