100-seed weight is a very complicated quantitative trait of yield. The study of gene mapping for yield trait in soybean is very important for application. However, the mapping result of 100-seed weight was dispersed, ...100-seed weight is a very complicated quantitative trait of yield. The study of gene mapping for yield trait in soybean is very important for application. However, the mapping result of 100-seed weight was dispersed, the public map should be chosen which was suitable for the published results integrated, and to improve yield. In this research, an integrated map of 100-seed weight QTLs in soybean had been established with soymap2 published in 2004 as a reference map. QTLs of 100-seed weight in soybean were collected in recent 20 yr. With the software BioMercator 2.1, QTLs from their own maps were projected to the reference map. From published papers, 65 QTLs of 100-seed weight were collected and 53 QTLs were integrated, including 17 reductive effect QTLs and 36 additive effect QTLs. 12 clusters of QTLs were found in the integrated map. A method of meta-analysis was used to narrow down the confidence interval, and 6 additive QTLs and 6 reductive QTLs and their corresponding markers were obtained respectively. The minimum confidence interval （C.I.） was shrunk to 1.52 cM. These results would lay the foundation for marker-assisted selection and mapping QTL precisely, as well as QTL gene cloning in soybean.展开更多
Birth weight(BW)and days to 100 kg(D100)are important economic traits that are both affected by polygenes.However,the genetic architecture of these quantitative traits is still elusive.Genotyping-by-sequencing(GBS)dat...Birth weight(BW)and days to 100 kg(D100)are important economic traits that are both affected by polygenes.However,the genetic architecture of these quantitative traits is still elusive.Genotyping-by-sequencing(GBS)data containing a large number of single nucleotide polymorphisms(SNPs)have become a powerful tool in genomic analysis.To better understand their complex genetic structure,a total of 600 Yorkshire pigs were sequenced using GBS technology.After quality control,279787 SNPs were generated for subsequent genome-wide association study(GWAS).A total of 30 genome-wide SNPs(P<1.79 E–07)were identified for D100.Furthermore,a total of 22 and 2 suggestive SNPs(P<3.57 E–06)were detected for D100 and BW,respectively.Of these,one locus located on SSC12(position:46226512 bp)were evaluated to affect both BW and D100 in Yorkshire pigs,indicating the pleiotropism in different traits.Considering the function of candidate genes,two genes,NSRP1 and DOCK7,were suggested as the most promising candidate genes involved in growth traits.Thus,use of GBS is able to identify novel variants and potential candidate genes for BW and D100,and provide an opportunity for improving pig growth traits using genomic selection in pigs.展开更多
Bambara groundnut (Vigna subterranea (L.) Verdc.) has economic importance for the producers and the traders as cash crop since it generates substantial income for households in Niger. The objective of this study was t...Bambara groundnut (Vigna subterranea (L.) Verdc.) has economic importance for the producers and the traders as cash crop since it generates substantial income for households in Niger. The objective of this study was to assess eight (8) Bambara groundnut morphotype from Université André Salifou de Zinder in Niger Republic by their morphological and agronomic characters. The experiment was conducted in a randomized complete block design with three replications during the rainy season of 2020-2021. Data were collected on the Number of leaves, Plant height (cm), Growth habit (cm), Terminal leaflet length, Terminal leaflet width, Number of nodes/stem, Number of stems/plant, Number of pods/plant, Weight of 100 pods (g), Weight of 100 seeds (g), Length of seeds (mm), Width of seeds (mm), Weight of seeds/Plot (kg). There were significant differences for all characters, except Number of leaves, Terminal leaflet length, Terminal leaflet width, Length of seeds (mm) Weight of 100 pods (g), Weight of 100 seeds (g). The morphotypes UZ-VZ-04, UZ-VZ-03, UZ-VZ-06, UZ-VZ-02 and UZ-VZ-05 have demonstrated good performance for grain weight per plot and can be useful for a breeding program.展开更多
基金supported by the Chinese Transgenic Specific Technology Programs (2009ZX08009-013B)
文摘100-seed weight is a very complicated quantitative trait of yield. The study of gene mapping for yield trait in soybean is very important for application. However, the mapping result of 100-seed weight was dispersed, the public map should be chosen which was suitable for the published results integrated, and to improve yield. In this research, an integrated map of 100-seed weight QTLs in soybean had been established with soymap2 published in 2004 as a reference map. QTLs of 100-seed weight in soybean were collected in recent 20 yr. With the software BioMercator 2.1, QTLs from their own maps were projected to the reference map. From published papers, 65 QTLs of 100-seed weight were collected and 53 QTLs were integrated, including 17 reductive effect QTLs and 36 additive effect QTLs. 12 clusters of QTLs were found in the integrated map. A method of meta-analysis was used to narrow down the confidence interval, and 6 additive QTLs and 6 reductive QTLs and their corresponding markers were obtained respectively. The minimum confidence interval （C.I.） was shrunk to 1.52 cM. These results would lay the foundation for marker-assisted selection and mapping QTL precisely, as well as QTL gene cloning in soybean.
基金supported by grants from the Sichuan Science and Technology Program,China(2020YFN0024)the Sichuan Innovation Team of Pig,Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province(sccxtd-2020-08)+2 种基金the National Key R&D Program of China(2018YFD0501204)the National Natural Science Foundation of China(31530073 and C170102)the the China Agricultural Research System of MOF and MARA(CARS-35-01A)。
文摘Birth weight(BW)and days to 100 kg(D100)are important economic traits that are both affected by polygenes.However,the genetic architecture of these quantitative traits is still elusive.Genotyping-by-sequencing(GBS)data containing a large number of single nucleotide polymorphisms(SNPs)have become a powerful tool in genomic analysis.To better understand their complex genetic structure,a total of 600 Yorkshire pigs were sequenced using GBS technology.After quality control,279787 SNPs were generated for subsequent genome-wide association study(GWAS).A total of 30 genome-wide SNPs(P<1.79 E–07)were identified for D100.Furthermore,a total of 22 and 2 suggestive SNPs(P<3.57 E–06)were detected for D100 and BW,respectively.Of these,one locus located on SSC12(position:46226512 bp)were evaluated to affect both BW and D100 in Yorkshire pigs,indicating the pleiotropism in different traits.Considering the function of candidate genes,two genes,NSRP1 and DOCK7,were suggested as the most promising candidate genes involved in growth traits.Thus,use of GBS is able to identify novel variants and potential candidate genes for BW and D100,and provide an opportunity for improving pig growth traits using genomic selection in pigs.
文摘Bambara groundnut (Vigna subterranea (L.) Verdc.) has economic importance for the producers and the traders as cash crop since it generates substantial income for households in Niger. The objective of this study was to assess eight (8) Bambara groundnut morphotype from Université André Salifou de Zinder in Niger Republic by their morphological and agronomic characters. The experiment was conducted in a randomized complete block design with three replications during the rainy season of 2020-2021. Data were collected on the Number of leaves, Plant height (cm), Growth habit (cm), Terminal leaflet length, Terminal leaflet width, Number of nodes/stem, Number of stems/plant, Number of pods/plant, Weight of 100 pods (g), Weight of 100 seeds (g), Length of seeds (mm), Width of seeds (mm), Weight of seeds/Plot (kg). There were significant differences for all characters, except Number of leaves, Terminal leaflet length, Terminal leaflet width, Length of seeds (mm) Weight of 100 pods (g), Weight of 100 seeds (g). The morphotypes UZ-VZ-04, UZ-VZ-03, UZ-VZ-06, UZ-VZ-02 and UZ-VZ-05 have demonstrated good performance for grain weight per plot and can be useful for a breeding program.
