[Objectives]This study was conducted to investigate the effect of tetrabromodiphenyl ether(BDE-47)on the activity of 11-ketotestosterone(11-KT)in tilapia liver,with a view to understanding the potential hazard of BDE-...[Objectives]This study was conducted to investigate the effect of tetrabromodiphenyl ether(BDE-47)on the activity of 11-ketotestosterone(11-KT)in tilapia liver,with a view to understanding the potential hazard of BDE-47 on fish and aquatic ecosystems from the perspective of sex steroid hormones.[Methods]Adopting the semi-static water exposure method,3 exposure concentrations of 5,50,and 500μg/L and 5 sampling time of 1,3,7,15,and 30 d were set to investigate the effect of BDE-47 on 11-ketotestosterone in tilapia liver.[Results]The low concentration of BDE-47(5μg/L)had no effect on the 11-KT level of tilapia liver;and when exposed to high concentrations of BDE-47(50 and 500μg/L),11-KT in the liver of tilapia was first suppressed and then returned to the normal level.Because the fish reproductive process is completed under the coordinated regulation of sex steroid hormones,significant changes of 11-KT in the liver of tilapia may cause its reproductive dysfunction to a certain extent.[Conclusions]This study provides relevant toxicological data for promoting the formulation(revision)of relevant water quality standards and the formulation of limit standards,and facilitating the protection of aquatic living resources and aquatic ecosystems.展开更多
SWEET(sugars will eventually be exported transporter)基因在植物开花过程中具有重要的作用,但AcSWEET11在菠萝成花中的作用机制尚不清楚。通过鉴定成花过程中与AcSWEET11的互作蛋白,为菠萝成花机制的解析奠定基础。本研究利用共转...SWEET(sugars will eventually be exported transporter)基因在植物开花过程中具有重要的作用,但AcSWEET11在菠萝成花中的作用机制尚不清楚。通过鉴定成花过程中与AcSWEET11的互作蛋白,为菠萝成花机制的解析奠定基础。本研究利用共转化的方法在菠萝成花过程的cDNA膜文库中筛选AcSWEET11的互作蛋白,分析候选蛋白的表达量。结果表明,pBT3-STE-AcSWEET11+pPR3-N对NMY51酵母细胞无毒性,但有自激活活性。进一步研究结果显示,在TDO/3?AT培养基和QDO培养基上自激活受到抑制。利用该系统筛选到了81个阳性克隆,经测序鉴定出48个与AcSWEET11互作的候选蛋白,包括E3 ubiquitin-protein ligase RING1-like、Trehalose-phosphate synthase 7、Cytochrome P450、TranscriptionfactorLUX等。GO和KEGG分析结果显示,48个蛋白主要分布在细胞进程、代谢过程、刺激反应和催化活性等生物过程,参与脂类代谢、氨基酸代谢和碳水化合物代谢、信号转导和运输与分解代谢等新陈代谢途径。Trehalose-phosphate synthase 7(XP_020105459.1)、Protein TIFY 3-like(XP_020082835.1)、40S ribosomal protein S27(XP_020092770.1)、Heterogeneous nuclear ribonucleoprotein 1-like(XP_020112516.1)等4个基因与AcSWEET11表达趋势一致,在菠萝成花过程中下调表达;Dihydrolipoyl dehydrogenase 2(XP_020113798.1)、Putative lipid-transfer protein DIR1(XP_020086640.1)、clathrin assembly protein At4g32285(XP_020108161.1)等3个基因在菠萝成花过程中上调表达。这些结果表明,AcSWEET11可能通过与Trehalose-phosphatesynthase7等蛋白发生互作,参与菠萝成花过程。本研究进一步丰富了AcSWEET11的蛋白互作网络,为AcSWEET11在菠萝成花中的调控机制的解析奠定基础。展开更多
基金National Key R&D Program(2020YFD0900502)Special Project of National Characteristic Freshwater Fish Industry Technology System(CARS-46).
文摘[Objectives]This study was conducted to investigate the effect of tetrabromodiphenyl ether(BDE-47)on the activity of 11-ketotestosterone(11-KT)in tilapia liver,with a view to understanding the potential hazard of BDE-47 on fish and aquatic ecosystems from the perspective of sex steroid hormones.[Methods]Adopting the semi-static water exposure method,3 exposure concentrations of 5,50,and 500μg/L and 5 sampling time of 1,3,7,15,and 30 d were set to investigate the effect of BDE-47 on 11-ketotestosterone in tilapia liver.[Results]The low concentration of BDE-47(5μg/L)had no effect on the 11-KT level of tilapia liver;and when exposed to high concentrations of BDE-47(50 and 500μg/L),11-KT in the liver of tilapia was first suppressed and then returned to the normal level.Because the fish reproductive process is completed under the coordinated regulation of sex steroid hormones,significant changes of 11-KT in the liver of tilapia may cause its reproductive dysfunction to a certain extent.[Conclusions]This study provides relevant toxicological data for promoting the formulation(revision)of relevant water quality standards and the formulation of limit standards,and facilitating the protection of aquatic living resources and aquatic ecosystems.
文摘SWEET(sugars will eventually be exported transporter)基因在植物开花过程中具有重要的作用,但AcSWEET11在菠萝成花中的作用机制尚不清楚。通过鉴定成花过程中与AcSWEET11的互作蛋白,为菠萝成花机制的解析奠定基础。本研究利用共转化的方法在菠萝成花过程的cDNA膜文库中筛选AcSWEET11的互作蛋白,分析候选蛋白的表达量。结果表明,pBT3-STE-AcSWEET11+pPR3-N对NMY51酵母细胞无毒性,但有自激活活性。进一步研究结果显示,在TDO/3?AT培养基和QDO培养基上自激活受到抑制。利用该系统筛选到了81个阳性克隆,经测序鉴定出48个与AcSWEET11互作的候选蛋白,包括E3 ubiquitin-protein ligase RING1-like、Trehalose-phosphate synthase 7、Cytochrome P450、TranscriptionfactorLUX等。GO和KEGG分析结果显示,48个蛋白主要分布在细胞进程、代谢过程、刺激反应和催化活性等生物过程,参与脂类代谢、氨基酸代谢和碳水化合物代谢、信号转导和运输与分解代谢等新陈代谢途径。Trehalose-phosphate synthase 7(XP_020105459.1)、Protein TIFY 3-like(XP_020082835.1)、40S ribosomal protein S27(XP_020092770.1)、Heterogeneous nuclear ribonucleoprotein 1-like(XP_020112516.1)等4个基因与AcSWEET11表达趋势一致,在菠萝成花过程中下调表达;Dihydrolipoyl dehydrogenase 2(XP_020113798.1)、Putative lipid-transfer protein DIR1(XP_020086640.1)、clathrin assembly protein At4g32285(XP_020108161.1)等3个基因在菠萝成花过程中上调表达。这些结果表明,AcSWEET11可能通过与Trehalose-phosphatesynthase7等蛋白发生互作,参与菠萝成花过程。本研究进一步丰富了AcSWEET11的蛋白互作网络,为AcSWEET11在菠萝成花中的调控机制的解析奠定基础。
