Objectives To assess the possible association between P2Y12 C34T、G52T polymorphism and clopidogrel resistance in coronary artery disease(CAD) of North China. Methods A case-control study was conducted in 615 patients...Objectives To assess the possible association between P2Y12 C34T、G52T polymorphism and clopidogrel resistance in coronary artery disease(CAD) of North China. Methods A case-control study was conducted in 615 patients with angiographically documented CAD.All 615 patients was detected platelet aggregation ratio,and accorded the platelet aggregation ratio to defined Clopidogrel resistance (CR) and None-Clopidogrel resistance(NCR),5μmol/L ADP-induced platelet aggregation ratio≥70%was defined CR.The P2Y12 C34T、G52T single nucleotide polymorphism was selected and genotyped.Results The genotype frequencies of CC,CT and TT of P2Y12 C34T polymorphism were 38.36%,50.68%and 10.96%in clopidogrel resistance patients, 50.96%,43.07%and 5.97%in non-clopidogrel resistance patients(P=0.010).The frequencies of P2Y12 C34T T allele frequencies in CR and NCR were 36.30%and 27.51% (P=0.004).The genotype frequencies of GG,GT and TT of P2Y12 G52T polymorphism were 74.66%,24.66%and 0.68%in clopidogrel resistance patients,79.74%,19.83% and 0.43%in non-clopidogrel resistance patients(P】0.05). The frequencies of P2Y12 G52T T allele frequencies in CR and NCR were 13.01%and 10.34%(P】0.05).After adjustment for conventional risk factors,including gender,age, body mass index,smoking status,hypertension,diabetes mellitus and hypercholesterolemia,there was significant difference between P2Y12 C34T polymorphism and clopidogel resistance (P =0.007).Conclusions The results suggest that P2Y12 C34T polymorphism might associated with clopidogrel resistance in CAD population of North China.展开更多
Objective To study the expression of bone matrix protein （BMP） induced by bovine bone morphogenetic proteins （BMPs） in vitro. Methods Type 1 collagen, osteopontin （OPN）, osteonectin （ON）, osteocalcin （OC）, a...Objective To study the expression of bone matrix protein （BMP） induced by bovine bone morphogenetic proteins （BMPs） in vitro. Methods Type 1 collagen, osteopontin （OPN）, osteonectin （ON）, osteocalcin （OC）, and bone sialoprotein （BSP） were detected by immunohistochemistry in C2C12 cultured from day 1 to day 28. Results The signaling of bone matrix protein expression became weaker except for type I collagen, OC and BSP after 5 days. Fourteen days after culture, the positive signaling of type I collagen, OPN, ON, OC, and BSP was gradually declined, and could be detected significantly as compared with that of the negative control on day 28. BMP assay showed that the lkaline phosphatase （ALP） activity was higher in C2C12 culture than in the control during the 14-day culture. Also, total protein and DNA significantly increased during the 14-day culture. High levels of ALP were seen in preosteoblasts and osteoblsts in vivo and in differentiating osteoblasts in vitro. ALP was well recognized as a marker reflecting osteoblastic activity. Conclusion Native bovine BMP induces conversion of myoblasts into osteoblasts, produces type I collagen, and plays significantly role in osteoinduction and bone matrix mineralization of C2C 12 in vitro.展开更多
Skeletal muscle satellite cells(C2C12)are origin of cells in mouse skeletal muscle,and used widely to explore the cell differentiation in sports science.C2C12 were cultured to explore the appropriate mechanical stimul...Skeletal muscle satellite cells(C2C12)are origin of cells in mouse skeletal muscle,and used widely to explore the cell differentiation in sports science.C2C12 were cultured to explore the appropriate mechanical stimulation conditions and lay a foundation for the establishment of a stable cell mechanical stimulation model in our study.C2C12 cells were cultured in vitro,purpose built centrifugal machine was used to exert mechanical stimulation through different revolving speed and duration.展开更多
文摘Objectives To assess the possible association between P2Y12 C34T、G52T polymorphism and clopidogrel resistance in coronary artery disease(CAD) of North China. Methods A case-control study was conducted in 615 patients with angiographically documented CAD.All 615 patients was detected platelet aggregation ratio,and accorded the platelet aggregation ratio to defined Clopidogrel resistance (CR) and None-Clopidogrel resistance(NCR),5μmol/L ADP-induced platelet aggregation ratio≥70%was defined CR.The P2Y12 C34T、G52T single nucleotide polymorphism was selected and genotyped.Results The genotype frequencies of CC,CT and TT of P2Y12 C34T polymorphism were 38.36%,50.68%and 10.96%in clopidogrel resistance patients, 50.96%,43.07%and 5.97%in non-clopidogrel resistance patients(P=0.010).The frequencies of P2Y12 C34T T allele frequencies in CR and NCR were 36.30%and 27.51% (P=0.004).The genotype frequencies of GG,GT and TT of P2Y12 G52T polymorphism were 74.66%,24.66%and 0.68%in clopidogrel resistance patients,79.74%,19.83% and 0.43%in non-clopidogrel resistance patients(P】0.05). The frequencies of P2Y12 G52T T allele frequencies in CR and NCR were 13.01%and 10.34%(P】0.05).After adjustment for conventional risk factors,including gender,age, body mass index,smoking status,hypertension,diabetes mellitus and hypercholesterolemia,there was significant difference between P2Y12 C34T polymorphism and clopidogel resistance (P =0.007).Conclusions The results suggest that P2Y12 C34T polymorphism might associated with clopidogrel resistance in CAD population of North China.
基金supported by the Ontario Research and Development Challenge Fund (ORDCF)GenSci Regeneration Sciences Inc. (Toronto,Canada)
文摘Objective To study the expression of bone matrix protein （BMP） induced by bovine bone morphogenetic proteins （BMPs） in vitro. Methods Type 1 collagen, osteopontin （OPN）, osteonectin （ON）, osteocalcin （OC）, and bone sialoprotein （BSP） were detected by immunohistochemistry in C2C12 cultured from day 1 to day 28. Results The signaling of bone matrix protein expression became weaker except for type I collagen, OC and BSP after 5 days. Fourteen days after culture, the positive signaling of type I collagen, OPN, ON, OC, and BSP was gradually declined, and could be detected significantly as compared with that of the negative control on day 28. BMP assay showed that the lkaline phosphatase （ALP） activity was higher in C2C12 culture than in the control during the 14-day culture. Also, total protein and DNA significantly increased during the 14-day culture. High levels of ALP were seen in preosteoblasts and osteoblsts in vivo and in differentiating osteoblasts in vitro. ALP was well recognized as a marker reflecting osteoblastic activity. Conclusion Native bovine BMP induces conversion of myoblasts into osteoblasts, produces type I collagen, and plays significantly role in osteoinduction and bone matrix mineralization of C2C 12 in vitro.
文摘Skeletal muscle satellite cells(C2C12)are origin of cells in mouse skeletal muscle,and used widely to explore the cell differentiation in sports science.C2C12 were cultured to explore the appropriate mechanical stimulation conditions and lay a foundation for the establishment of a stable cell mechanical stimulation model in our study.C2C12 cells were cultured in vitro,purpose built centrifugal machine was used to exert mechanical stimulation through different revolving speed and duration.
基金jointly supported by the National Natural Science Foundation of China(No.22273093,No.41905018,No.21903080)the Ministry of Science and Technology of China(No.2022YFF0606500)。
文摘目的探讨新生儿持续肺动脉高压(persistent pulmonary hypertension of newborn,PPHN)患儿血清人CXC型趋化因子配体8(C-X-C motif chemokine ligand 8,CXCL8)、CXCL12与一氧化氮吸入治疗临床转归的关系。方法选择2021-08/2023-05月作者医院收治并给予一氧化氮吸入治疗的PPHN患儿135例为研究对象。根据患儿出院时临床转归结局分为死亡组(n=32)和存活组(n=103)。比较两组PPHN患儿血清CXCL8、CXCL12水平。单因素及多因素Logistic回归模型分析接受一氧化氮吸入治疗PPHN患儿临床转归的影响因素。受试者工作特征(receiver operating characteristic,ROC)曲线分析血清CXCL8、CXCL12对接受一氧化氮吸入治疗PPHN患儿临床转归的预测价值。结果死亡组患儿血清CXCL8、CXCL12水平显著高于存活组(P均<0.05)。多因素Logsitic回归分析结果显示,血清CXCL8水平升高、血清CXCL12水平升高、早产、出生时Apgar评分0~3分、合并并发症是接受一氧化氮吸入治疗的PPHN患儿死亡的危险因素,肺表面活性物质应用、吸入一氧化氮早期反应则是保护因素(P<0.05)。ROC曲线分析结果显示,血清CXCL8、CXCL12联合检测对接受一氧化氮吸入治疗的PPHN患儿死亡预测的曲线下面积(area under the curve,AUC)为0.828,大于血清CXCL8、CXCL12单独检测(AUC分别为0.762、0.714)。结论PPHN患儿血清CXCL8、CXCL12水平升高与接受一氧化氮治疗的不良临床转归有关,且CXCL8、CXCL12水平升高是PPHN患儿死亡的危险因素。CXCL8、CXCL12联合检测对接受一氧化氮治疗PPHN患儿死亡具有较高的预测价值。
