We present new photometry for two contact binaries,Y Sex and V1363 Ori,which were observed by three small telescopes in China.By using the W-D method,the absolute parameters are updated from new BVR light curves and p...We present new photometry for two contact binaries,Y Sex and V1363 Ori,which were observed by three small telescopes in China.By using the W-D method,the absolute parameters are updated from new BVR light curves and previous radial velocity curves.Results identify that two binaries are deep,lowmass ratio(DLMR)overcontact binaries with q≤0.25 and f≥50%.From the temperature-luminosity diagram,the primary components are slightly evolved main-sequence stars,whose evolutionary ages are~2.51 Gyr for Y Sex and~3.56 Gyr for V 1363 Ori,respectively.From the(O-C)curves,it is found that the orbital periods may be undergoing secular increase with cyclic variations,which may be interpreted either by magnetic activity cycles or by the light-time orbit effect.With period increasing,this kind of DLMR overcontact binaries,such as Y Sex and V1363 Ori,will evolve into the rapid-rotating single stars.展开更多
Objective To discuss the feasibility of pereutaneous nephrolithotomy ( PCNL) for treating upper urinary calculi under local anesthesia. Methods One thousand three hundred and sixty - three patients who suffered
To evaluate the specific immune responses induced by recombinant Lactococcus lactis（L.lactis） which expresses porcine epidemic diarrhea virus（PEDV） S1 protein through oral administration,the spike gene fragment of...To evaluate the specific immune responses induced by recombinant Lactococcus lactis（L.lactis） which expresses porcine epidemic diarrhea virus（PEDV） S1 protein through oral administration,the spike gene fragment of PEDV was amplified from PEDV SDLY strain to construct p MG36 e-S1 recombinant plasmid.The recombinant plasmid was then electro-transferred into competent cells of L.lactis MG1363,to prepare the recombinant L.lactis expressing S1 protein of PEDV.The expression of target protein was identified by SDS-PAGE and Western-blot.New Zealand white rabbits were orally administered with the recombinant strain;the antibody titer in intestinal mucosa and serum was detected by neutralizing test;and the specific Ig G in serum was evaluated by indirect ELISA.The results showed that the recombinant L.lactis could effectively induce high level of Ig G in serum and high level of mucosal immune antibody.The recombinant L.lactis is qualified to be a potential oral vaccine because it could successfully stimulate both humoral and mucosal immune responses against PEDV.展开更多
基金the National Natural Science Foundation of China(No.11873003)the Natural Science Research Key Program of Anhui Provincial Department of Education(No.kj2019A0954)+1 种基金the Outstanding Young Talents Program of Anhui Provincial Department of Education(gxgnfx2019084)the Open Project Program of the Key Laboratory of Optical Astronomy of NAOC。
文摘We present new photometry for two contact binaries,Y Sex and V1363 Ori,which were observed by three small telescopes in China.By using the W-D method,the absolute parameters are updated from new BVR light curves and previous radial velocity curves.Results identify that two binaries are deep,lowmass ratio(DLMR)overcontact binaries with q≤0.25 and f≥50%.From the temperature-luminosity diagram,the primary components are slightly evolved main-sequence stars,whose evolutionary ages are~2.51 Gyr for Y Sex and~3.56 Gyr for V 1363 Ori,respectively.From the(O-C)curves,it is found that the orbital periods may be undergoing secular increase with cyclic variations,which may be interpreted either by magnetic activity cycles or by the light-time orbit effect.With period increasing,this kind of DLMR overcontact binaries,such as Y Sex and V1363 Ori,will evolve into the rapid-rotating single stars.
文摘Objective To discuss the feasibility of pereutaneous nephrolithotomy ( PCNL) for treating upper urinary calculi under local anesthesia. Methods One thousand three hundred and sixty - three patients who suffered
基金Supported by Priority Academic Talent Team Cultivation Program of Shandong Colleges and Universities and Agricultural Industry Research System of Shandong Province(SDAIT-06-022-08)People’s Livelihood Science and Technology Program of Qingdao City(16-6-2-42-nsh)
文摘To evaluate the specific immune responses induced by recombinant Lactococcus lactis（L.lactis） which expresses porcine epidemic diarrhea virus（PEDV） S1 protein through oral administration,the spike gene fragment of PEDV was amplified from PEDV SDLY strain to construct p MG36 e-S1 recombinant plasmid.The recombinant plasmid was then electro-transferred into competent cells of L.lactis MG1363,to prepare the recombinant L.lactis expressing S1 protein of PEDV.The expression of target protein was identified by SDS-PAGE and Western-blot.New Zealand white rabbits were orally administered with the recombinant strain;the antibody titer in intestinal mucosa and serum was detected by neutralizing test;and the specific Ig G in serum was evaluated by indirect ELISA.The results showed that the recombinant L.lactis could effectively induce high level of Ig G in serum and high level of mucosal immune antibody.The recombinant L.lactis is qualified to be a potential oral vaccine because it could successfully stimulate both humoral and mucosal immune responses against PEDV.
