AIM: To investigate the influence of peroxisome proliferator activated receptor γ (PPARγ) ligand, 15-deoxy-△12, 14-prostaglandin J2 (15dPGJ2) on the proliferation and apoptosis of MCG-803 human gastric cancer cell ...AIM: To investigate the influence of peroxisome proliferator activated receptor γ (PPARγ) ligand, 15-deoxy-△12, 14-prostaglandin J2 (15dPGJ2) on the proliferation and apoptosis of MCG-803 human gastric cancer cell lines.METHODS: Cell proliferation was measured by 3H-TdR assay. Apoptosis was determined by ELISA and TUNEL staining. Protein and mRNA level of bcl-2 family and COXs were measured by Western blotting and Northern blotting respectively. PGE2 production was examined by RIA.RESULTS: 15dPGJ2 inhibited cell growth and induced apoptosis of MlCG-803 cells. The COX-2 and bcl-2/bax ratios were decreased following 15dPGJ2 treatment. The PGE2production in supernatants was also decreased. These changes were in a dose-dependent manner.CONCLUSION: 15dPGJ2 may be a useful therapeutic agent for the treatment of gastric cancer.展开更多
Prostaglandin J2 (PG J2) and its metabolites are naturally occurring derivatives of Prostaglandin D2 (PG D2). The pathway for formation of these compounds involves sequential conversion of PG D2 to PG J2, and 15-deoxy...Prostaglandin J2 (PG J2) and its metabolites are naturally occurring derivatives of Prostaglandin D2 (PG D2). The pathway for formation of these compounds involves sequential conversion of PG D2 to PG J2, and 15-deoxy-△12,14-prostaglandin-J2 (15d-PGJ2). 15d-PGJ2 is a high-affinity ligand for peroxisome proliferation-activated receptor gamma (PPAR7), it represses several genes in different cell lines. Our previous studies have demonstrated that 15d-PGJ2 induced apoptosis in ECV304 endothelial cells. However, the mechanism remains unclear. In this paper, our aim was to explore the molecular mechanism of 15d-PGJ2 on apoptosis in ECV304 endothelial cells. Hoechst 33258 staining, terminal deoxynucleotidyl transferase-展开更多
As a ligand-dependent transcriptional factor, peroxisome proliferator-activated receptor （PPAR） γ was expressed in several human cancer cells and PPARγ activation could induce cells growth inhibition. In this stud...As a ligand-dependent transcriptional factor, peroxisome proliferator-activated receptor （PPAR） γ was expressed in several human cancer cells and PPARγ activation could induce cells growth inhibition. In this study we investigated the PPARγ expression in colon cancer cell line HT-29 and checked the inducement of apoptosis by two PPARγ specific agonists rosiglitazone and 15d-PGJ2. In addition, we also observed the protein expressions of PTEN and p-Akt1 before and after treatment with rosiglitazone and examination whether activation of PPARγ could increase the expression of PTEN in colon cancer cells.展开更多
基金Science Fund of Guangdong Province,No.015012Science Fund of Guangzhou,2001-Z-01-2the State 973 Projects,2002ccc0400
文摘AIM: To investigate the influence of peroxisome proliferator activated receptor γ (PPARγ) ligand, 15-deoxy-△12, 14-prostaglandin J2 (15dPGJ2) on the proliferation and apoptosis of MCG-803 human gastric cancer cell lines.METHODS: Cell proliferation was measured by 3H-TdR assay. Apoptosis was determined by ELISA and TUNEL staining. Protein and mRNA level of bcl-2 family and COXs were measured by Western blotting and Northern blotting respectively. PGE2 production was examined by RIA.RESULTS: 15dPGJ2 inhibited cell growth and induced apoptosis of MlCG-803 cells. The COX-2 and bcl-2/bax ratios were decreased following 15dPGJ2 treatment. The PGE2production in supernatants was also decreased. These changes were in a dose-dependent manner.CONCLUSION: 15dPGJ2 may be a useful therapeutic agent for the treatment of gastric cancer.
文摘Prostaglandin J2 (PG J2) and its metabolites are naturally occurring derivatives of Prostaglandin D2 (PG D2). The pathway for formation of these compounds involves sequential conversion of PG D2 to PG J2, and 15-deoxy-△12,14-prostaglandin-J2 (15d-PGJ2). 15d-PGJ2 is a high-affinity ligand for peroxisome proliferation-activated receptor gamma (PPAR7), it represses several genes in different cell lines. Our previous studies have demonstrated that 15d-PGJ2 induced apoptosis in ECV304 endothelial cells. However, the mechanism remains unclear. In this paper, our aim was to explore the molecular mechanism of 15d-PGJ2 on apoptosis in ECV304 endothelial cells. Hoechst 33258 staining, terminal deoxynucleotidyl transferase-
基金This work was supported by a grant from the 135 Medical ImportantTalent Foundation of Jiangsu Province (No.37RC2002037).
文摘As a ligand-dependent transcriptional factor, peroxisome proliferator-activated receptor （PPAR） γ was expressed in several human cancer cells and PPARγ activation could induce cells growth inhibition. In this study we investigated the PPARγ expression in colon cancer cell line HT-29 and checked the inducement of apoptosis by two PPARγ specific agonists rosiglitazone and 15d-PGJ2. In addition, we also observed the protein expressions of PTEN and p-Akt1 before and after treatment with rosiglitazone and examination whether activation of PPARγ could increase the expression of PTEN in colon cancer cells.
