Nucleotide sequences from a 500 bp region of the 16SrRNA gene were analyzed for ten insect pests of five different orders to examine the patterns of variation within the gene fragment and the taxonomic levels for whic...Nucleotide sequences from a 500 bp region of the 16SrRNA gene were analyzed for ten insect pests of five different orders to examine the patterns of variation within the gene fragment and the taxonomic levels for which it showed maximum utility in phylogeny estimation. A hierarchical approach was adopted in the study through comparison of levels of sequence variation among taxa at different taxonomic levels. Among them, partial 16SrRNA gene was amplified in ten insects of five different orders. As previously reported for many holometabolous insects, the 16SrRNA gene data is reported here for 5 different orders were highly AT-rich and exhibited strong site-to-site variation in substitution rate. The partial 16SrRNA genes of five out of ten insects were reported first time. Primers were made from blasting 2 different genera of the order Diptera. These primers were proven to be universal as it amplified the partial 16SrRNA gene in ten different insects across five different orders, Diptera, Coleoptera, Heteroptera, Lepidoptera and Hymenoptera. Later, a phylogenetic tree was also constructed for understanding and analyzing the relation of above five orders. This study resulted in unusual findings which were as follows: All the species of Drosophila of order Diptera were evolutionary more closely related to Dysdercus koenigii of order Heteroptera than Bactrocera cucurbitae of Drosophilan order, Diptera in terms of partial 16SrRNA gene sequence. Similarly, Spodoptera litura and Helicoverpa armigera belonged to same family Noctuidae whereas Pieris brassicae belonged to family Pieridae. All belonged to order Lepidoptera. The results showed that Spodoptera litura in terms of partial 16SrRNA gene sequence was evolutionary more close to Pieris brassicae than Helicoverpa armigera.展开更多
This study investigated differences in the community structure and environmental responses of the bacterial community in sediments of the Bohai Sea.Illumina high-throughput sequencing technology and real-time PCR were...This study investigated differences in the community structure and environmental responses of the bacterial community in sediments of the Bohai Sea.Illumina high-throughput sequencing technology and real-time PCR were used to assay the bacterial 16S rRNA genes in the surface sediments of 13 sampling stations in the Bohai Sea.The results showed that sediments at the majority of the 13 sampling stations were contaminated by heavy metal mercury.The main phyla of bacteria recorded included Proteobacteria(52.92%),Bacteroidetes(11.76%),Planctomycetes(7.39%),Acidobacteria(6.53%)and Chloroflexi(4.97%).The genus with the highest relative abundance was Desulfobulbus(4.99%),which was the dominant genus at most sampling stations,followed by Lutimonas and Halioglobus.The main factors influencing bacterial community structure were total organic carbon,followed by depth and total phosphorus.The content of lead,cadmium,chromium,copper and zinc had a consistent effect on community structure.Arsenic showed a negative correlation with bacterial community structure in most samples,while the impact of mercury on community structure was not significant.The bacterial community in sediment samples from the Bohai Sea was rich in diversity and displayed an increase in diversity from high to low latitudes.The data indicated that the Bohai Sea had abundant microbial resources and was rich in bacteria with the potential to metabolize many types of pollutants.展开更多
文摘Nucleotide sequences from a 500 bp region of the 16SrRNA gene were analyzed for ten insect pests of five different orders to examine the patterns of variation within the gene fragment and the taxonomic levels for which it showed maximum utility in phylogeny estimation. A hierarchical approach was adopted in the study through comparison of levels of sequence variation among taxa at different taxonomic levels. Among them, partial 16SrRNA gene was amplified in ten insects of five different orders. As previously reported for many holometabolous insects, the 16SrRNA gene data is reported here for 5 different orders were highly AT-rich and exhibited strong site-to-site variation in substitution rate. The partial 16SrRNA genes of five out of ten insects were reported first time. Primers were made from blasting 2 different genera of the order Diptera. These primers were proven to be universal as it amplified the partial 16SrRNA gene in ten different insects across five different orders, Diptera, Coleoptera, Heteroptera, Lepidoptera and Hymenoptera. Later, a phylogenetic tree was also constructed for understanding and analyzing the relation of above five orders. This study resulted in unusual findings which were as follows: All the species of Drosophila of order Diptera were evolutionary more closely related to Dysdercus koenigii of order Heteroptera than Bactrocera cucurbitae of Drosophilan order, Diptera in terms of partial 16SrRNA gene sequence. Similarly, Spodoptera litura and Helicoverpa armigera belonged to same family Noctuidae whereas Pieris brassicae belonged to family Pieridae. All belonged to order Lepidoptera. The results showed that Spodoptera litura in terms of partial 16SrRNA gene sequence was evolutionary more close to Pieris brassicae than Helicoverpa armigera.
基金The National Key Basic Research Special Foundation of China under contract No.2017YFC1404500the National Natural Science Foundation of China under contract No.41676115
文摘This study investigated differences in the community structure and environmental responses of the bacterial community in sediments of the Bohai Sea.Illumina high-throughput sequencing technology and real-time PCR were used to assay the bacterial 16S rRNA genes in the surface sediments of 13 sampling stations in the Bohai Sea.The results showed that sediments at the majority of the 13 sampling stations were contaminated by heavy metal mercury.The main phyla of bacteria recorded included Proteobacteria(52.92%),Bacteroidetes(11.76%),Planctomycetes(7.39%),Acidobacteria(6.53%)and Chloroflexi(4.97%).The genus with the highest relative abundance was Desulfobulbus(4.99%),which was the dominant genus at most sampling stations,followed by Lutimonas and Halioglobus.The main factors influencing bacterial community structure were total organic carbon,followed by depth and total phosphorus.The content of lead,cadmium,chromium,copper and zinc had a consistent effect on community structure.Arsenic showed a negative correlation with bacterial community structure in most samples,while the impact of mercury on community structure was not significant.The bacterial community in sediment samples from the Bohai Sea was rich in diversity and displayed an increase in diversity from high to low latitudes.The data indicated that the Bohai Sea had abundant microbial resources and was rich in bacteria with the potential to metabolize many types of pollutants.
