A method was developed for the simultaneous determination of four kinds of estrogens （hexoestrol, diethylstilbestrol, estrone, and 17-beta-estradiol） in feed by gas chromatography-mass spectrometry （GC-MS）. After ...A method was developed for the simultaneous determination of four kinds of estrogens （hexoestrol, diethylstilbestrol, estrone, and 17-beta-estradiol） in feed by gas chromatography-mass spectrometry （GC-MS）. After the sample was extracted by ethyl ether and cleaned-up on HLB phase extraction column, four kinds of estrogens were derived and quantified in gas chromatographymass spectrometry. The results showed that the linear detectable ranged from 2.5 ng· mL-1 to 250 ng· mL-1for hexoestrol and from 5 ng· mL-1 to 500 ng· mL-1 for three other estrogens with the correlation coefficients （R2） were no less than 0.990. The recoveries were in the range of 76.34%-96.33% and the relative standard deviation was no more than 22.7%. The limits of quantitation （LOQ） for all analytics were between 10 ug· kg^-1 and 20 ug· kg^-1. The method was accurate and sensitive and could meet the actual requirements for the analyses of feed samples.展开更多
Objective:To evaluate the effects of 17-beta-estradiol on growth factors of goldfish(Carassius auratus).Methods:To perform the test,17-beta-estradiol was given 3 months period to fish at different doses as followed:co...Objective:To evaluate the effects of 17-beta-estradiol on growth factors of goldfish(Carassius auratus).Methods:To perform the test,17-beta-estradiol was given 3 months period to fish at different doses as followed:control group,Group 1:10 mg/kg food,Group 2:25 mg/kg food and Group 3:50 mg/kg food.For this purpose,a solution of hormone in pure ethanol used to spray on food.Feeding was done 3 times daily as an appetite.Comparing the mean values measured for length and weight using ANOVA.Results:Indicated with increase length and weight,the effects of the hormone get more distinct,so that with increase concentration of hormone,reduce weight and length.Conclusions:Estradiol along with testosterone and progesterone regulates final stages of oocyte maturation and ovulation.Various studies have proven the different concentrations of this hormone has different effects on the growth of different fishes.The aim of this study was to assess the effects of this hormone on growth factors of Carassius auratus.展开更多
基金Supported by Fund of Harbin Provincial Education Department(2014AB3BN041)
文摘A method was developed for the simultaneous determination of four kinds of estrogens （hexoestrol, diethylstilbestrol, estrone, and 17-beta-estradiol） in feed by gas chromatography-mass spectrometry （GC-MS）. After the sample was extracted by ethyl ether and cleaned-up on HLB phase extraction column, four kinds of estrogens were derived and quantified in gas chromatographymass spectrometry. The results showed that the linear detectable ranged from 2.5 ng· mL-1 to 250 ng· mL-1for hexoestrol and from 5 ng· mL-1 to 500 ng· mL-1 for three other estrogens with the correlation coefficients （R2） were no less than 0.990. The recoveries were in the range of 76.34%-96.33% and the relative standard deviation was no more than 22.7%. The limits of quantitation （LOQ） for all analytics were between 10 ug· kg^-1 and 20 ug· kg^-1. The method was accurate and sensitive and could meet the actual requirements for the analyses of feed samples.
基金Supported by the Gorgan University of Agriculture Science&Natural Resource(Grant No.6177515-5).
文摘Objective:To evaluate the effects of 17-beta-estradiol on growth factors of goldfish(Carassius auratus).Methods:To perform the test,17-beta-estradiol was given 3 months period to fish at different doses as followed:control group,Group 1:10 mg/kg food,Group 2:25 mg/kg food and Group 3:50 mg/kg food.For this purpose,a solution of hormone in pure ethanol used to spray on food.Feeding was done 3 times daily as an appetite.Comparing the mean values measured for length and weight using ANOVA.Results:Indicated with increase length and weight,the effects of the hormone get more distinct,so that with increase concentration of hormone,reduce weight and length.Conclusions:Estradiol along with testosterone and progesterone regulates final stages of oocyte maturation and ovulation.Various studies have proven the different concentrations of this hormone has different effects on the growth of different fishes.The aim of this study was to assess the effects of this hormone on growth factors of Carassius auratus.
