2,4-二硝基氯苯洗涤废水资源化研究

采用活性炭吸附-碱液脱附法无害化处理2,4-二硝基氯苯[CDNB]生产中的废水,使CDNB的含量降到0.5 mg/L以下,达到国家地表水一级排放最高含量0.5 mg/L的标准(GB 3838-2002).活性炭可以反复再生,重复利用,处理废水成本由10元/t降低到2元/t以下.将CDNB转化成2,4-二硝基苯酚[DNP]进行回收,既避免了二次污染,又变废为宝.

竹炭对溶液中2,4-二硝基氯苯的吸附与回收研究

本研究了竹炭对溶液中2,4-二硝基氯苯(CDNB)的吸附行为。研究确定竹炭对CDNB的吸附平衡时间、最佳吸附pH以及吸附温度、吸附剂用量等因素对吸附效果的影响。实验结果表明:CDNB初始浓度c0=0.05mg/mL,T=298K,pH=5.0,吸附时间t=12h时,竹炭对CDNB的吸附率E可达到97.6%;用乙醇作洗脱再生剂,洗脱率可达95.2%;且脱附的CDNB可用蒸馏重结晶法回收,回收率可达86.4%。因此,竹炭是废水中CDNB吸附处理的材料。

Reductive transformation and detoxification mechanism of 2,4-dinitrochlorobenzene in combined zero valent iron and anaerobic-aerobic process

A combined zero valent iron （ZVI） and anaerobic-aerobic process was adopted for the treatment of 2,4-dinitrochlorobenzene （DNCB）- containing wastewater. The transformation pathway, reduction of acute toxicity and enhancement of biodegradability were investigated, After pretreatment by ZVI, DNCB in wastewater could be completely converted into 2,4-diaminochlorobenzene （DACB）. The ratio of BODs/COD increased from 0.005±0.001 to 0.168±0.007, while EC50,48hr （V/V） increased from 0.65% to 5.20%, indicating the enhancement of biodegradability and reduction of acute toxicity with the pretreatment by ZVI. DACB was further dechlorinated to m-phenylenediamine during the anaerobic process using methanol as electron donor, with EC50＇ 48 hr increasing from 5.20% to 48.2%. After the subsequent anaerobic-aerobic process, m-phenylenediamine was degraded completely, with effluent COD of 67.5±10.8 mg/L. This effluent of the subsequent anaerobic-aerobic process was not toxic to zebrafish. The combined ZVI and anaerobic-aerobic process offers bright prospects for the treatment of chlorinated nitroaromatic compound-containing wastewater.

柱前衍生-高效液相色谱法测定大熊猫血清中18种游离氨基酸

建立了大熊猫血清中18种游离氨基酸的反相高效液相色谱分析方法。血清样品经HPO3沉淀蛋白后,在弱碱性条件（pH 9.0）下游离氨基酸与2,4-二硝基氯苯在90℃水浴中避光反应1.5 h,生成具有紫外吸收的衍生物,用反相C18柱分离,紫外检测器检测,内标法定量。18种氨基酸的工作曲线的相关系数范围为0.9951～0.9999;相对标准偏差为1.4%～4.7%;检出限为0.20～7.72μg/mL;加标回收率为65.9%～118%。

A new chronic ulcerative colitis model produced by combined methods in rats

INTRODUCTIONThe etiopathogenesis of ulcerative colitis (UC)remains obscure,due to lack of an ideal animalmodel.With thc improvement of theory andmethodology in the last 30 years,people used toadopt chemicals(acetic acid,ethanol,carrageen,etc.),immunotechniques(humoral or cellularimmunity,immune complex) and substance derivedfrom UC patients to set up various kinds of UCanimal model,which mimic the pathologic changesof human UC,so far these remain far

玉米螟谷胱甘肽转硫酶的纯化及性质研究

以亚洲玉米螟（Ｏｓｔｒｉｎｉａｆｕｒｎａｃａｌｉｓ）为材料，经ＳｅｐｈａｄｅｘＧ７５、ＤＥＡＥＳｅｐｈａｒｏｓｅＦａｓｔＦｌｏｗ、还原型谷胱甘肽（ＧＳＨ）为配基的亲和层析，分离纯化得到玉米螟谷胱甘肽转硫酶（ＧＳＴ）。以２，４二硝基氯苯（ＣＤＮＢ）为底物测得其比活为１０７０Ｕ／ｍｇ蛋白，收率为２２９％，提纯１１３８倍。经ＳＤＳＰＡＧＥ和ＰＡＧＥ鉴定，得到的ＧＳＴ为一条带，亚基的分子量为２５ｋＤ，经凝胶过滤测得其全酶分子量为５０ｋＤ，说明该酶是由两个相同亚基组成的。ＩＥＦ测得主带等电点为８２。该酶最适温度为４１℃，最适ｐＨ范围为６３～６９。经动力学测定该酶双底物反应机制为序列机制，对ＣＤＮＢ的Ｋｍ值为０１５ｍｍｏｌ／Ｌ，对ＧＳＨ的Ｋｍ值为０３５ｍｍｏｌ／Ｌ，两种底物的ｋｃａｔ均为７８３ｓ－１。Ｃｌ－对底物ＣＤＮＢ有竞争性抑制作用，对ＧＳＨ是非竞争性抑制作用；３，５二硝基苯甲酸（ＤＮＢ）对ＣＤＮＢ有竞争性抑制作用，对ＧＳＨ是反竞争性抑制作用。