K^(+)cycling in the cochlea is critical to maintain hearing.Many sodium-potassium pumps are proved to participate in K^(+)cycling,such as Na/K-ATPase.Theα2-Na/K-ATPase is an important isoform of Na/K-ATPase.The expre...K^(+)cycling in the cochlea is critical to maintain hearing.Many sodium-potassium pumps are proved to participate in K^(+)cycling,such as Na/K-ATPase.Theα2-Na/K-ATPase is an important isoform of Na/K-ATPase.The expression ofα2-Na/K-ATPase in the cochlea is not clear.In this study,we used C57BL/6 mice as a model of presbycusis and implemented immunohistochemistry staining and quantitative real time-PCR,and theα2-Na/K-ATPase expression pattern was confirmed in the inner ear.It was foundα2-Na/K-ATPase was expressed widely in cochlea and its mRNA and protein expression was gradually reduced with aging(4-,14-,26-and 48-weeks old mice).We suspected that,the down-regulation ofα2-Na/K-ATPase expression might be associated with the remodeling of K^(+)cycling,degeneration of morphological structure and decrease of hearing function in aging C57 mice.In conclusion,we speculated that the reduction ofα2-Na/K-ATPase might play an important role in the pathogenesis of age-related hearing loss.展开更多
The results from this study showed that the thresholds of brainstem auditory-evoked potentials peak following 10 successive days of intramuscular injection of Roman chickens with kanamycin, starting 3 days after birth...The results from this study showed that the thresholds of brainstem auditory-evoked potentials peak following 10 successive days of intramuscular injection of Roman chickens with kanamycin, starting 3 days after birth. Fluorescence immunohistochemistry analysis revealed few ganglion cells positively labeled for Ephrin A2 in the cochlea of experimental chickens from 2 days before until 7 days after the last kanamycin injection. The number of Ephrin A2-positive ganglion cell bodies was increased at 15 days after the last injection and was similar to that in normal chickens at 30 days following the cessation of kanamycin treatment. These experimental findings indicate that Ephrin A2 protein expression in the acoustic ganglia is synchronized with the connection damage and regeneration of cochlear hair cells after kanamycin exposure. Ephrin A2 may play an important role in the regeneration and plasticity of cochlear hair cells in the chick cochlea following kanamycin ototoxicity.展开更多
目的观察耳穴埋豆联合柴术汤治疗2型糖尿病(Type 2 diabetes mellitus,T2DM)伴抑郁患者的临床疗效及对神经内分泌指标的影响。方法选取2019年9月—2020年5月期间石家庄市中医院收治的90例T2DM伴抑郁患者,按简单随机化法分为治疗组、对照...目的观察耳穴埋豆联合柴术汤治疗2型糖尿病(Type 2 diabetes mellitus,T2DM)伴抑郁患者的临床疗效及对神经内分泌指标的影响。方法选取2019年9月—2020年5月期间石家庄市中医院收治的90例T2DM伴抑郁患者,按简单随机化法分为治疗组、对照1组和对照2组,各30例。治疗组采取氟哌噻吨美利曲辛片联合柴术汤、耳穴埋豆,对照1组采取氟哌噻吨美利曲辛片联合柴术汤,对照2组采取氟哌噻吨美利曲辛片。治疗3个月后,比较3组患者治疗效果、中医证候积分(心情抑郁、胸肋胀满、心烦易怒)、汉密尔顿抑郁量表24项(Hamilton depresssion scale-24,HAMD-24)、血糖指标[糖化血红蛋白(Gglycosylated hemoglobin,HbA1c)、空腹血糖(Fasting plasma glucose,FPG)、餐后2 h血糖(2 h blood glucose after meal,2 h PG)]、神经内分泌指标[5-羟色氨酸(5-hydroxytryptamine,5-HT)、多巴胺(Dopamine,DA)、促肾上腺皮质激素(Adrenocorticotrophic hormone,ACTH)]及不良反应情况。结果治疗3个月后,治疗组总有效率93.10%(27/29)高于对照2组55.17%(16/29)、对照1组65.52%(19/29),差异有统计学意义(P<0.05);对照2组和对照1组治疗总有效率比较,差异无统计学意义(P>0.05)。治疗3个月后3组患者中医证候积分及HAMD-24评分均低于治疗前,差异有统计学意义(P<0.05);且治疗组HAMD-24及心情抑郁、心烦易怒、胸肋胀满评分均低于对照2组、对照1组,对照1组低于对照2组,差异有统计学意义(P<0.05)。治疗3个月后3组患者FPG、2 h PG、HbA1c水平均低于治疗前,差异有统计学意义(P<0.05);且治疗组FPG、2 h PG、HbA1c水平均低于对照2组、对照1组,对照1组低于对照2组,差异有统计学意义(P<0.05)。治疗3个月后3组患者5-HT、DA水平均高于治疗前,ACTH水平均低于治疗前,差异有统计学意义(P<0.05);且治疗组5-HT、DA水平均高于对照2组、对照1组,ACTH水平均低于对照2组、对照1组,对照1组5-HT、DA水平均高于对照2组,ACTH水平低于对照2组,差异有统计学意义(P<0.05)。3组患者不良反应发生率比较,差异无统计学意义(P>0.05)。结论耳穴埋豆联合柴术汤有利于控制T2DM伴抑郁患者血糖和抑郁,提高治疗效果,且安全性高,机制可能与调节神经内分泌有关。展开更多
基金National Natural Science Foundation of China(Nos.81771004,81271078,81500791,81500794 and 81300827).
文摘K^(+)cycling in the cochlea is critical to maintain hearing.Many sodium-potassium pumps are proved to participate in K^(+)cycling,such as Na/K-ATPase.Theα2-Na/K-ATPase is an important isoform of Na/K-ATPase.The expression ofα2-Na/K-ATPase in the cochlea is not clear.In this study,we used C57BL/6 mice as a model of presbycusis and implemented immunohistochemistry staining and quantitative real time-PCR,and theα2-Na/K-ATPase expression pattern was confirmed in the inner ear.It was foundα2-Na/K-ATPase was expressed widely in cochlea and its mRNA and protein expression was gradually reduced with aging(4-,14-,26-and 48-weeks old mice).We suspected that,the down-regulation ofα2-Na/K-ATPase expression might be associated with the remodeling of K^(+)cycling,degeneration of morphological structure and decrease of hearing function in aging C57 mice.In conclusion,we speculated that the reduction ofα2-Na/K-ATPase might play an important role in the pathogenesis of age-related hearing loss.
基金supported by the Natural Science Foundation of Shanghai,No.08ZR1414900 and 11ZR1423600
文摘The results from this study showed that the thresholds of brainstem auditory-evoked potentials peak following 10 successive days of intramuscular injection of Roman chickens with kanamycin, starting 3 days after birth. Fluorescence immunohistochemistry analysis revealed few ganglion cells positively labeled for Ephrin A2 in the cochlea of experimental chickens from 2 days before until 7 days after the last kanamycin injection. The number of Ephrin A2-positive ganglion cell bodies was increased at 15 days after the last injection and was similar to that in normal chickens at 30 days following the cessation of kanamycin treatment. These experimental findings indicate that Ephrin A2 protein expression in the acoustic ganglia is synchronized with the connection damage and regeneration of cochlear hair cells after kanamycin exposure. Ephrin A2 may play an important role in the regeneration and plasticity of cochlear hair cells in the chick cochlea following kanamycin ototoxicity.
文摘目的观察耳穴埋豆联合柴术汤治疗2型糖尿病(Type 2 diabetes mellitus,T2DM)伴抑郁患者的临床疗效及对神经内分泌指标的影响。方法选取2019年9月—2020年5月期间石家庄市中医院收治的90例T2DM伴抑郁患者,按简单随机化法分为治疗组、对照1组和对照2组,各30例。治疗组采取氟哌噻吨美利曲辛片联合柴术汤、耳穴埋豆,对照1组采取氟哌噻吨美利曲辛片联合柴术汤,对照2组采取氟哌噻吨美利曲辛片。治疗3个月后,比较3组患者治疗效果、中医证候积分(心情抑郁、胸肋胀满、心烦易怒)、汉密尔顿抑郁量表24项(Hamilton depresssion scale-24,HAMD-24)、血糖指标[糖化血红蛋白(Gglycosylated hemoglobin,HbA1c)、空腹血糖(Fasting plasma glucose,FPG)、餐后2 h血糖(2 h blood glucose after meal,2 h PG)]、神经内分泌指标[5-羟色氨酸(5-hydroxytryptamine,5-HT)、多巴胺(Dopamine,DA)、促肾上腺皮质激素(Adrenocorticotrophic hormone,ACTH)]及不良反应情况。结果治疗3个月后,治疗组总有效率93.10%(27/29)高于对照2组55.17%(16/29)、对照1组65.52%(19/29),差异有统计学意义(P<0.05);对照2组和对照1组治疗总有效率比较,差异无统计学意义(P>0.05)。治疗3个月后3组患者中医证候积分及HAMD-24评分均低于治疗前,差异有统计学意义(P<0.05);且治疗组HAMD-24及心情抑郁、心烦易怒、胸肋胀满评分均低于对照2组、对照1组,对照1组低于对照2组,差异有统计学意义(P<0.05)。治疗3个月后3组患者FPG、2 h PG、HbA1c水平均低于治疗前,差异有统计学意义(P<0.05);且治疗组FPG、2 h PG、HbA1c水平均低于对照2组、对照1组,对照1组低于对照2组,差异有统计学意义(P<0.05)。治疗3个月后3组患者5-HT、DA水平均高于治疗前,ACTH水平均低于治疗前,差异有统计学意义(P<0.05);且治疗组5-HT、DA水平均高于对照2组、对照1组,ACTH水平均低于对照2组、对照1组,对照1组5-HT、DA水平均高于对照2组,ACTH水平低于对照2组,差异有统计学意义(P<0.05)。3组患者不良反应发生率比较,差异无统计学意义(P>0.05)。结论耳穴埋豆联合柴术汤有利于控制T2DM伴抑郁患者血糖和抑郁,提高治疗效果,且安全性高,机制可能与调节神经内分泌有关。
