Esters of 2- and 3-monochloropropane-1,2-diol (MCPD) are significative contaminants of processed edible oils used as foods or food ingredients. The aim of this study was to develop and validate a new method by GC-MS f...Esters of 2- and 3-monochloropropane-1,2-diol (MCPD) are significative contaminants of processed edible oils used as foods or food ingredients. The aim of this study was to develop and validate a new method by GC-MS for the simultaneous quantification of 2 and 3-MCPD esters in infant milk powder and edible vegetable oils. The developed protocol included fat fraction in infant milk powder and edible vegetable oils samples was extracted and treated with sodium methylate-methanol to cleave the ester bonds of the 2- and 3-MCPD esters, moreover, standard samples of deuterium isotope-labeled 2- and 3-MCPD palmitic acid double esters and stearic acid double esters were used as the internal standards. Furthermore, this method was validated when it was applied to food products, concrete manifestation in its good accuracy (the recovery of MCPD esters ranged from 86% to 114%), high sensitivity (the LOD of 3-MCPD and 2-MCPD esters were 0.025 and 0.020 mg/kg, LOQ were 0.075, 0.060 mg/kg, respectively) and satisfactory repeatability (RSD below 6.8%) for all analytes. In the 150 commercial edible vegetable oils and infant formula milk powder samples, we obtained a preliminary profile of MCPD ester contamination.展开更多
A new phloretin derivative 1 3-[2-(4-hydroxy-phenyl)-ethyl]-benzo[d] isoxazole-4,6-diol (yield 63%) was synthesized from phloretin by carbonyl nucleophilic addition condensation reaction. Its structure was characteriz...A new phloretin derivative 1 3-[2-(4-hydroxy-phenyl)-ethyl]-benzo[d] isoxazole-4,6-diol (yield 63%) was synthesized from phloretin by carbonyl nucleophilic addition condensation reaction. Its structure was characterized by 1H NMR, 13C NMR and HR-MS. The phloretin, compound 1, resveratrol and acetylated resveratrol were determined by comparing them with paclitaxel. Anti-tumor activity of alcohol on SPC-A1, EC109, A549, MCF-7 and MDA-MB-231 cell lines. Compound 1 showed better antitumor activity than docetaxel against A549 tumor cells.展开更多
Ni/Al_2O_3-SiO_2 catalysts were synthesized via one-step method employing SiO_2 as an additive for the selective hydrogenation of butyne-1,4-diol(B_3D) to butane-1,4-diol(B1D). The prepared catalysts were evaluated by...Ni/Al_2O_3-SiO_2 catalysts were synthesized via one-step method employing SiO_2 as an additive for the selective hydrogenation of butyne-1,4-diol(B_3D) to butane-1,4-diol(B1D). The prepared catalysts were evaluated by a series of characterization techniques including BET, XRD, SEM, EDX-mapping, TEM, H_2-TPR, XPS, NH_3-TPD and Py-FTIR. Compared to Ni/Al_2O_3 catalyst, the SiO_2-doped samples exhibited better B_3D conversion. SiO_2 could help to form a strong interaction between NiO with the support, which inhibited Ni agglomeration at high temperature, improved the Ni dispersion, and enhanced the hydrogenation activity. B_1D selectivity was mainly influenced by the quantity of Lewis acid sites in addition to the Ni dispersion. The catalyst with a silica loading of 6.4% demonstrated an excellent selectivity of 75.18%(by 13% higher than the contrastive Ni/Al_2O_3 catalyst), which was attributed to the larger amount of Lewis acid sites and the moderate interaction between NiO with the support, which could facilitate the nickel dispersion on a preferable surface area of 176.3 m^2/g of support.展开更多
Objective:To evaluate the in-vitro antioxidant and antimicrobial activity of cvcloart-23- ene-3β,25-diol(called as B2) isolated from stem bark of Pongamia pinnata.Methods:In vitro antioxidant activity of B2 was deter...Objective:To evaluate the in-vitro antioxidant and antimicrobial activity of cvcloart-23- ene-3β,25-diol(called as B2) isolated from stem bark of Pongamia pinnata.Methods:In vitro antioxidant activity of B2 was determined by methods for determination of Dl’PH radical scavenging,reducing power,superoxide anion radical scavenging,hydroxyl radical scavenging, hydrogen peroxide scavenging,metal chelating and nitric oxide radical scavenging at the doses of 20.40.60.80 and 100μg/mL,respectively.3 -tocopherol with same concentration was used as a standard antioxidant.In vitro antimicrobial activity of B2 was determined by cup plate method in different concentration range of 10-100μg/mL.Results:The results indicated that dose dependent%reduction against DPPH radical,reducing power,superoxide anion radical scavenging,hydroxyl radical scavenging,metal chelating,hydrogen peroxide scavenging and nitric oxide radical scavenging by B2 andβ-tocopherol.Conclusions:It is concluded that cycloart 23-ene-3β,25 diol(B2) showed dose dependent antioxidant activity.B2 showed more DPPH radical scavenging,reducing power,superoxide scavenging,hydroxyl radical scavenging, metal chelating scavenging,hydrogen peroxide radical scavenging and nitric oxide radical scavenging activity thanβ-tocopherol and in case of antimicrobial activity B2 exhibited broad-spectrum activ ity against bacteria and strong activity against yeast type of fungi.展开更多
ObjectiveTo investigate the effects of friedelin (terpenoid) and 8-hydroxyisocapnolactone-2-3-diol (coumarin) with concentration 10 μM, 30 μM, and 100 μM on inhibiting mast cells (MCs) degranulation....ObjectiveTo investigate the effects of friedelin (terpenoid) and 8-hydroxyisocapnolactone-2-3-diol (coumarin) with concentration 10 μM, 30 μM, and 100 μM on inhibiting mast cells (MCs) degranulation.MethodsThe investigation was performed in vitro by administering each compound into rat peritoneal MCs and rat basophilic leukemia-2H3 cells followed by activation with 50 μg/mL of compound 48/80 or 1 μM of ionomycin. The concentration of histamine released from each group was measured by a high-performance liquid chromatography-fluorometry system with post-column derivatization using o-phthalaldehyde.Results8-Hydroxyisocapnolactone-2-3-diol inhibited degranulation of compound 48/80 activated-rat peritoneal MCs with the histamine release percentages of 74.57%, 72.21% and 51.79% when the 10 μM, 30 μM and 100 μM concentrations were used, respectively. Where as about 81% histamine was released by the control group. Degranulation inhibition ability was also observed in ionomycin-activated rat basophilic leukemia-2H3 cells. In contrast, friedelin failed to inhibit degranulation in either cell type. The inhibition of 8-hydroxyisocapnolactone-2-3-diol was not related to the depletion of histamine synthesis as implied by the total histamine measurement.ConclusionsThese results exhibit the promising of 8-hydroxyisocapnolactone-2-3-diol is a potential parent structure for developing a MCs stabilizer.展开更多
The title compound 3,9-bis（pyridin-2-yl）-2,4,8,10-tetraoxaspiro[5.5]undecane 4（C17H18N2O4） has been synthesized by the reaction of 2,2-bis（hydroxymethyl）propane-1,3-diol with pyridine-2-carbaldehyde in the prese...The title compound 3,9-bis（pyridin-2-yl）-2,4,8,10-tetraoxaspiro[5.5]undecane 4（C17H18N2O4） has been synthesized by the reaction of 2,2-bis（hydroxymethyl）propane-1,3-diol with pyridine-2-carbaldehyde in the presence of p-toluenesulfonic acid,and characterized by IR,1H-NMR and X-ray single-crystal diffraction.The crystal belongs to monoclinic system,space group C2/c with a = 25.133（2）,b = 5.7219（5）,c = 21.942（2）（A°） ,β = 99.145（2）°,V = 3115.3（5）（A°）^3,Z = 8,Dc = 1.340 g/cm^3,Mr = 314.33,F（000） = 1328,μ = 0.097 mm-1,MoKa radiation（λ = 0.71073）,R = 0.0352 and wR = 0.1022 for 2486 observed reflections with I 〉 2σ（I）.X-ray analysis reveals that the two 1,3-dioxane rings passing through the central spiro-C atom both adopt chair conformation.Intermolecular C-H…π,C-H…O and C-H…N weak interactions link the molecules to form a three-dimensional samdwich,which are effective in the stabilization of the crystal structure.展开更多
Environmental concerns have highlighted the need of cleaner technologies. A cleaner, convenient and selective technology has been developed for the preparation of 3-(4,6-dimethoxy-pyrimidin-2-yloxy)-2-methyl-phenol (...Environmental concerns have highlighted the need of cleaner technologies. A cleaner, convenient and selective technology has been developed for the preparation of 3-(4,6-dimethoxy-pyrimidin-2-yloxy)-2-methyl-phenol (DPMP) from 2-methyl-benzene-1,3-diol in water as solvent. The isolated yield of DPMP is up to 86% in good selectivity. The product structure was characterized by conventional methods, H-NMR and MS. This technology belongs to the modern concept of 1 environmentally friendly low wastes or non-wastes technology (LWNWT).展开更多
The compound （1 z）-2-（hydroxymethyl）-dodec-1-ene-1,3-diol was previously isolated from the integument of the red palm weevil Rhynhophorus ferruginous and found to antagonize calcium chloride induced dysrrhythmia i...The compound （1 z）-2-（hydroxymethyl）-dodec-1-ene-1,3-diol was previously isolated from the integument of the red palm weevil Rhynhophorus ferruginous and found to antagonize calcium chloride induced dysrrhythmia in mice. Further calcium-dependent pharmacological actions performed in this study revealed that it has the ability to inhibit platelets aggregation induced in guinea pigs with either ADP or archegonia acids, and the compound also decreased the arterial pressure in rats in a dose-dependent manner and exerted potential ant consultant activity in mice against pentylene tetrazole-induced seizures. However, no complete protection was observed. Analgesic studies in mice using the hot plate （58 ℃） revealed its potential analgesic activities. Its effective dose 50 （ED50） was 85.7 ± 6.3 mg/kg （N= 5）. The compound seamed to produce all of the above actions via a calcium antagonistic action.展开更多
A new 3-（3,4-methylenedioxyphenyl）-propane-1,2-diol glycoside,named cinnamomdiol A（1）,together with two known compounds,3-（3,4-methylenedioxyphenyl）-propane-1,2-diol（2） and taxifolin（3）,was isolated from the...A new 3-（3,4-methylenedioxyphenyl）-propane-1,2-diol glycoside,named cinnamomdiol A（1）,together with two known compounds,3-（3,4-methylenedioxyphenyl）-propane-1,2-diol（2） and taxifolin（3）,was isolated from the roots of Cinnamomum camphora.Their structures were determined on the basis of spectroscopic evidences.Compounds 2 and 3 were isolated from the title plant for the first time.展开更多
运用色谱分离技术,从泽泻科泽泻中分离得到7个化学成分,其结构用各种光谱技术(包括红外光谱、质谱、一维和二维核磁共振谱)鉴定为4-pyrazin-2-yl-but-3-ene-1,2-diol,烟酰胺,甘油棕榈酸酯,1-monolinolein,11-deoxyalisol,alisol A 24-ac...运用色谱分离技术,从泽泻科泽泻中分离得到7个化学成分,其结构用各种光谱技术(包括红外光谱、质谱、一维和二维核磁共振谱)鉴定为4-pyrazin-2-yl-but-3-ene-1,2-diol,烟酰胺,甘油棕榈酸酯,1-monolinolein,11-deoxyalisol,alisol A 24-acetate及alisol C 23-acetate.其中,4-pyrazin-2-yl-but-3-ene-1,2-diol、烟酰胺、甘油棕榈酸酯和1-monolinolein为首次从泽泻科中获得.展开更多
A new alkaloid, named 6-（2＇,3＇-dihydroxy-4＇-hydroxymethyl-tetrahydro-furan-1＇-yl）cyclopentadiene[c]pyrrole-1,3-diol, was isolated from the seeds of Castanea mollissima Blume. The structure was elucidated based o...A new alkaloid, named 6-（2＇,3＇-dihydroxy-4＇-hydroxymethyl-tetrahydro-furan-1＇-yl）cyclopentadiene[c]pyrrole-1,3-diol, was isolated from the seeds of Castanea mollissima Blume. The structure was elucidated based on spectroscopic evidence including 2D NMR techniques.展开更多
文摘Esters of 2- and 3-monochloropropane-1,2-diol (MCPD) are significative contaminants of processed edible oils used as foods or food ingredients. The aim of this study was to develop and validate a new method by GC-MS for the simultaneous quantification of 2 and 3-MCPD esters in infant milk powder and edible vegetable oils. The developed protocol included fat fraction in infant milk powder and edible vegetable oils samples was extracted and treated with sodium methylate-methanol to cleave the ester bonds of the 2- and 3-MCPD esters, moreover, standard samples of deuterium isotope-labeled 2- and 3-MCPD palmitic acid double esters and stearic acid double esters were used as the internal standards. Furthermore, this method was validated when it was applied to food products, concrete manifestation in its good accuracy (the recovery of MCPD esters ranged from 86% to 114%), high sensitivity (the LOD of 3-MCPD and 2-MCPD esters were 0.025 and 0.020 mg/kg, LOQ were 0.075, 0.060 mg/kg, respectively) and satisfactory repeatability (RSD below 6.8%) for all analytes. In the 150 commercial edible vegetable oils and infant formula milk powder samples, we obtained a preliminary profile of MCPD ester contamination.
文摘A new phloretin derivative 1 3-[2-(4-hydroxy-phenyl)-ethyl]-benzo[d] isoxazole-4,6-diol (yield 63%) was synthesized from phloretin by carbonyl nucleophilic addition condensation reaction. Its structure was characterized by 1H NMR, 13C NMR and HR-MS. The phloretin, compound 1, resveratrol and acetylated resveratrol were determined by comparing them with paclitaxel. Anti-tumor activity of alcohol on SPC-A1, EC109, A549, MCF-7 and MDA-MB-231 cell lines. Compound 1 showed better antitumor activity than docetaxel against A549 tumor cells.
基金Financial support from the National Natural Science Foundation of China (21163019) is gratefully acknowledged
文摘Ni/Al_2O_3-SiO_2 catalysts were synthesized via one-step method employing SiO_2 as an additive for the selective hydrogenation of butyne-1,4-diol(B_3D) to butane-1,4-diol(B1D). The prepared catalysts were evaluated by a series of characterization techniques including BET, XRD, SEM, EDX-mapping, TEM, H_2-TPR, XPS, NH_3-TPD and Py-FTIR. Compared to Ni/Al_2O_3 catalyst, the SiO_2-doped samples exhibited better B_3D conversion. SiO_2 could help to form a strong interaction between NiO with the support, which inhibited Ni agglomeration at high temperature, improved the Ni dispersion, and enhanced the hydrogenation activity. B_1D selectivity was mainly influenced by the quantity of Lewis acid sites in addition to the Ni dispersion. The catalyst with a silica loading of 6.4% demonstrated an excellent selectivity of 75.18%(by 13% higher than the contrastive Ni/Al_2O_3 catalyst), which was attributed to the larger amount of Lewis acid sites and the moderate interaction between NiO with the support, which could facilitate the nickel dispersion on a preferable surface area of 176.3 m^2/g of support.
文摘Objective:To evaluate the in-vitro antioxidant and antimicrobial activity of cvcloart-23- ene-3β,25-diol(called as B2) isolated from stem bark of Pongamia pinnata.Methods:In vitro antioxidant activity of B2 was determined by methods for determination of Dl’PH radical scavenging,reducing power,superoxide anion radical scavenging,hydroxyl radical scavenging, hydrogen peroxide scavenging,metal chelating and nitric oxide radical scavenging at the doses of 20.40.60.80 and 100μg/mL,respectively.3 -tocopherol with same concentration was used as a standard antioxidant.In vitro antimicrobial activity of B2 was determined by cup plate method in different concentration range of 10-100μg/mL.Results:The results indicated that dose dependent%reduction against DPPH radical,reducing power,superoxide anion radical scavenging,hydroxyl radical scavenging,metal chelating,hydrogen peroxide scavenging and nitric oxide radical scavenging by B2 andβ-tocopherol.Conclusions:It is concluded that cycloart 23-ene-3β,25 diol(B2) showed dose dependent antioxidant activity.B2 showed more DPPH radical scavenging,reducing power,superoxide scavenging,hydroxyl radical scavenging, metal chelating scavenging,hydrogen peroxide radical scavenging and nitric oxide radical scavenging activity thanβ-tocopherol and in case of antimicrobial activity B2 exhibited broad-spectrum activ ity against bacteria and strong activity against yeast type of fungi.
文摘ObjectiveTo investigate the effects of friedelin (terpenoid) and 8-hydroxyisocapnolactone-2-3-diol (coumarin) with concentration 10 μM, 30 μM, and 100 μM on inhibiting mast cells (MCs) degranulation.MethodsThe investigation was performed in vitro by administering each compound into rat peritoneal MCs and rat basophilic leukemia-2H3 cells followed by activation with 50 μg/mL of compound 48/80 or 1 μM of ionomycin. The concentration of histamine released from each group was measured by a high-performance liquid chromatography-fluorometry system with post-column derivatization using o-phthalaldehyde.Results8-Hydroxyisocapnolactone-2-3-diol inhibited degranulation of compound 48/80 activated-rat peritoneal MCs with the histamine release percentages of 74.57%, 72.21% and 51.79% when the 10 μM, 30 μM and 100 μM concentrations were used, respectively. Where as about 81% histamine was released by the control group. Degranulation inhibition ability was also observed in ionomycin-activated rat basophilic leukemia-2H3 cells. In contrast, friedelin failed to inhibit degranulation in either cell type. The inhibition of 8-hydroxyisocapnolactone-2-3-diol was not related to the depletion of histamine synthesis as implied by the total histamine measurement.ConclusionsThese results exhibit the promising of 8-hydroxyisocapnolactone-2-3-diol is a potential parent structure for developing a MCs stabilizer.
基金supported by the National Natural Science Foundation of China (No. 20872051 and 21002009)the Scientific and Technological Project of Changzhou (CJ20115019)the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘The title compound 3,9-bis（pyridin-2-yl）-2,4,8,10-tetraoxaspiro[5.5]undecane 4（C17H18N2O4） has been synthesized by the reaction of 2,2-bis（hydroxymethyl）propane-1,3-diol with pyridine-2-carbaldehyde in the presence of p-toluenesulfonic acid,and characterized by IR,1H-NMR and X-ray single-crystal diffraction.The crystal belongs to monoclinic system,space group C2/c with a = 25.133（2）,b = 5.7219（5）,c = 21.942（2）（A°） ,β = 99.145（2）°,V = 3115.3（5）（A°）^3,Z = 8,Dc = 1.340 g/cm^3,Mr = 314.33,F（000） = 1328,μ = 0.097 mm-1,MoKa radiation（λ = 0.71073）,R = 0.0352 and wR = 0.1022 for 2486 observed reflections with I 〉 2σ（I）.X-ray analysis reveals that the two 1,3-dioxane rings passing through the central spiro-C atom both adopt chair conformation.Intermolecular C-H…π,C-H…O and C-H…N weak interactions link the molecules to form a three-dimensional samdwich,which are effective in the stabilization of the crystal structure.
文摘Environmental concerns have highlighted the need of cleaner technologies. A cleaner, convenient and selective technology has been developed for the preparation of 3-(4,6-dimethoxy-pyrimidin-2-yloxy)-2-methyl-phenol (DPMP) from 2-methyl-benzene-1,3-diol in water as solvent. The isolated yield of DPMP is up to 86% in good selectivity. The product structure was characterized by conventional methods, H-NMR and MS. This technology belongs to the modern concept of 1 environmentally friendly low wastes or non-wastes technology (LWNWT).
文摘The compound （1 z）-2-（hydroxymethyl）-dodec-1-ene-1,3-diol was previously isolated from the integument of the red palm weevil Rhynhophorus ferruginous and found to antagonize calcium chloride induced dysrrhythmia in mice. Further calcium-dependent pharmacological actions performed in this study revealed that it has the ability to inhibit platelets aggregation induced in guinea pigs with either ADP or archegonia acids, and the compound also decreased the arterial pressure in rats in a dose-dependent manner and exerted potential ant consultant activity in mice against pentylene tetrazole-induced seizures. However, no complete protection was observed. Analgesic studies in mice using the hot plate （58 ℃） revealed its potential analgesic activities. Its effective dose 50 （ED50） was 85.7 ± 6.3 mg/kg （N= 5）. The compound seamed to produce all of the above actions via a calcium antagonistic action.
文摘A new 3-（3,4-methylenedioxyphenyl）-propane-1,2-diol glycoside,named cinnamomdiol A（1）,together with two known compounds,3-（3,4-methylenedioxyphenyl）-propane-1,2-diol（2） and taxifolin（3）,was isolated from the roots of Cinnamomum camphora.Their structures were determined on the basis of spectroscopic evidences.Compounds 2 and 3 were isolated from the title plant for the first time.
文摘运用色谱分离技术,从泽泻科泽泻中分离得到7个化学成分,其结构用各种光谱技术(包括红外光谱、质谱、一维和二维核磁共振谱)鉴定为4-pyrazin-2-yl-but-3-ene-1,2-diol,烟酰胺,甘油棕榈酸酯,1-monolinolein,11-deoxyalisol,alisol A 24-acetate及alisol C 23-acetate.其中,4-pyrazin-2-yl-but-3-ene-1,2-diol、烟酰胺、甘油棕榈酸酯和1-monolinolein为首次从泽泻科中获得.
文摘A new alkaloid, named 6-（2＇,3＇-dihydroxy-4＇-hydroxymethyl-tetrahydro-furan-1＇-yl）cyclopentadiene[c]pyrrole-1,3-diol, was isolated from the seeds of Castanea mollissima Blume. The structure was elucidated based on spectroscopic evidence including 2D NMR techniques.
