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Reduction of uncertainties in rice yield response to elevated CO_(2) by experiment-model integration:A case study in East China
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作者 Zihao Wang Yu Zhang +2 位作者 Xueni Wang Yanfeng Ding Songhan Wang 《The Crop Journal》 SCIE CSCD 2024年第6期1812-1816,共5页
Accurate prediction of future rice yield needs the precise estimations of rice yield response to climate change factors,of which the most important one is the increasing carbon dioxide(CO_(2))concentrations.Estimates ... Accurate prediction of future rice yield needs the precise estimations of rice yield response to climate change factors,of which the most important one is the increasing carbon dioxide(CO_(2))concentrations.Estimates of CO_(2) fertilization effect(CFE)on rice,however,still had large uncertainties.Therefore,using the rice planting areas in East China as the study area,we firstly compared the rice yields and CFE predicted by four state-of-the-art crop models,and found that the CFE predicted by these models had significant differences.We then quantified the CFE on rice yield using the field-controlled experiment conducted at Danyang site at Jiangsu province.Using CFE measurements from a field experiment as benchmark,we have developed an experiment–model integration approach aiming to reduce this variation.This study thus highlights the large CFE uncertainties of current crop models and provides us with a method to reduce this uncertainty,which is beneficial for the accurate prediction of future global rice yield in the context of climate change. 展开更多
关键词 Rice yield Elevated CO_(2) experiment-model integration Field experiment Crop models
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Laboratory Experiments Do Not Support the Greenhouse Effect as Applicable to CO2
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作者 Michael D. Nelson 《International Journal of Geosciences》 CAS 2024年第10期809-839,共31页
The analysis of Greenhouse Effect experiments in the public domain indicated that the lab tests were primarily centered around illustrating the mechanics of conventional greenhouses. They used high-energy visible ligh... The analysis of Greenhouse Effect experiments in the public domain indicated that the lab tests were primarily centered around illustrating the mechanics of conventional greenhouses. They used high-energy visible light (such as sunlight), rather than addressing the Greenhouse Effect, which involves low-energy infrared radiation emitted by the Earth’s surface. Studies with argon, a non-greenhouse gas with similar density to CO2, showed thermal heat transfer as the dominant factor in the temperature profiles, with radiation absorption being undetected. The same conclusion was drawn by another study, which measured infrared back radiation. Experiments using exaggerated CO2 concentrations inadvertently illustrated the principle of the Popper Falsification Test by disproving the Greenhouse Effect applicable to CO2 within the troposphere. A straightforward kitchen test showed that a microwave oven cannot be used as a model for the Greenhouse Effect. 展开更多
关键词 Laboratory experiment Climate Change Greenhouse Effect CO2
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内皮细胞特异性骨形态发生蛋白2对血管新生的影响:生物信息学分析和实验验证
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作者 燕茹 王凯茹 +2 位作者 张飞燕 贾绍斌 丛广志 《中国组织工程研究》 CAS 北大核心 2025年第1期103-110,共8页
背景:血管新生是心血管疾病的主要干预靶点,骨形态发生蛋白2具有调控血管新生作用,但内皮细胞特异性骨形态发生蛋白2对血管新生的调控作用不清楚。目的:探讨内皮细胞特异性骨形态发生蛋白2对血管新生的影响。方法:(1)生物信息学分析:通... 背景:血管新生是心血管疾病的主要干预靶点,骨形态发生蛋白2具有调控血管新生作用,但内皮细胞特异性骨形态发生蛋白2对血管新生的调控作用不清楚。目的:探讨内皮细胞特异性骨形态发生蛋白2对血管新生的影响。方法:(1)生物信息学分析:通过Panglao DB公共基因表达数据库单细胞转录组荟萃分析观察骨形态发生蛋白2细胞群表达丰度和定位。血管新生小鼠和内皮(心内膜)过表达骨形态发生蛋白2小鼠转录组测序数据集探索内皮细胞骨形态发生蛋白2对血管新生信号通路的调控作用。(2)体内实验验证:建立小鼠后肢缺血模型,对比模型小鼠患侧与健侧缺血后肢7,14和21 d血流灌注情况,免疫荧光和免疫组织化学染色评估小鼠骨形态发生蛋白2和CD31的表达定位情况。(3)体外实验验证:体外培养人脐静脉内皮细胞,分为对照组、缺氧组和骨形态发生蛋白2抑制剂(Noggin蛋白)干预组,培养24 h,观察各组内皮细胞血管新生情况。结果与结论:(1)内皮细胞是表达骨形态发生蛋白2的重要细胞亚群,在血管新生内皮细胞和骨形态发生蛋白2过表达内皮细胞转录组再分析均发现骨形态发生蛋白2表达明显升高,血管新生通路明显激活。(2)缺血7 d小鼠新生血管周围骨形态发生蛋白2阳性血管明显增加(P<0.05),缺血2周骨形态发生蛋白2阳性血管明显减少(P<0.001)。(3)体外培养人脐静脉内皮细胞,缺氧干预后,内皮细胞迁移能力和血管出芽明显增加,血管新生因子血管内皮生长因子和血小板衍生生长因子的表达明显升高,Noggin明显减少了缺氧诱导的内皮细胞血管新生(P<0.001),并下调血管内皮生长因子和血小板衍生生长因子的表达(P<0.01)。(4)结果证实,内皮细胞特异性骨形态发生蛋白2具有调控血管新生作用,靶向性内皮细胞骨形态发生蛋白2可望改善血管新生。 展开更多
关键词 内皮细胞 骨形态发生蛋白2 血管新生 单细胞RNA测序 批量RNA测序 信号通路 后肢缺血模型 成管实验
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注CO_(2)驱提高致密砂岩气藏采收率实验及数值模拟
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作者 廖海婴 《大庆石油地质与开发》 北大核心 2025年第1期101-106,共6页
针对致密砂岩气藏地层压力下降快、稳产难度大、采收率较低等问题,利用长岩心驱替实验、可视化实验和数值模拟方法,从提高气藏采收率方面对注CO_(2)的驱替特征和扩散特征进行了研究。结果表明:地层中的超临界CO_(2)不是与天然气大范围... 针对致密砂岩气藏地层压力下降快、稳产难度大、采收率较低等问题,利用长岩心驱替实验、可视化实验和数值模拟方法,从提高气藏采收率方面对注CO_(2)的驱替特征和扩散特征进行了研究。结果表明:地层中的超临界CO_(2)不是与天然气大范围地互溶,而是“部分互溶”;由于重力分异作用,CO_(2)在沉降过程中驱替天然气向上运移并被采出;CO_(2)驱替天然气可提高采收率17.8百分点。研究成果可为下一步致密砂岩气藏CO_(2)驱矿场试验提供理论依据。 展开更多
关键词 CO_(2)驱 致密气藏 砂岩气藏 可视化实验 提高采收率 数值模拟
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Remarks on interpretations of the Eotvos experiment and misinterpretation of E=mc^2 被引量:1
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作者 鲁重贤 《Chinese Physics B》 SCIE EI CAS CSCD 2007年第3期635-639,共5页
The Eotvos experiment on the verification of equivalence between inertial mass and gravitational mass of a body is famous for its accuracy. A question is, however, can these experimental results be applied to the case... The Eotvos experiment on the verification of equivalence between inertial mass and gravitational mass of a body is famous for its accuracy. A question is, however, can these experimental results be applied to the case of a physical space in general relativity, where the space coordinates could be arbitrary? It is pointed out that it can be validly applied because it has been proven that Einstein's equivalence principle for a physical space must have a frame of reference with the Euclidean-like structure. Will claimed further that such an overall accuracy can be translated into an accuracy of the equivalence between inertial mass and each type of energy. It is shown that, according to general relativity, such a claim is incorrect. The root of this problem is due to an inadequate understanding of special relativity that produced the famous equation E=mc^2, which must be understood in terms of energy conservation. Concurrently, it is pointed out that this error is a problem in Will's book, ‘Theory and Experiment in Gravitational Physics'. 展开更多
关键词 Einstein's equivalence principle Einstein-Minkowski condition Euclidean-like structure Eotvos experiment weak equivalence principle E=MC^2
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Simulation and Experiment for Oxygen-enriched Combustion Engine Using Liquid Oxygen to Solidify CO2 被引量:5
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作者 LIU Yongfeng JIA Xiaoshe +3 位作者 PEI Pucheng LU Yong YI Li SHI Yan 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2016年第1期188-194,共7页
For capturing and recycling of CO2 in the internal combustion engine, Rankle cycle engine can reduce the exhaust pollutants effectively under the condition of ensuring the engine thermal efficiency by using the techni... For capturing and recycling of CO2 in the internal combustion engine, Rankle cycle engine can reduce the exhaust pollutants effectively under the condition of ensuring the engine thermal efficiency by using the techniques of spraying water in the cylinder and optimizing the ignition advance angle. However, due to the water spray nozzle need to be installed on the cylinder, which increases the cylinder head design difficulty and makes the combustion conditions become more complicated. In this paper, a new method is presented to carry out the closing inlet and exhaust system for internal combustion engines. The proposed new method uses liquid oxygen to solidify part of cooled CO2 from exhaust system into dry ice and the liquid oxygen turns into gas oxygen which is sent to inlet system. The other part of CO2 is sent to inlet system and mixed with oxygen, which can reduce the oxygen-enriched combustion detonation tendency and make combustion stable. Computing grid of the IP52FMI single-cylinder four-stroke gasoline-engine is established according to the actual shape of the combustion chamber using KIVA-3V program. The effects of exhaust gas recirculation (EGR) rate are analyzed on the temperatures, the pressures and the instantaneous heat release rates when the EGR rate is more than 8%. The possibility of enclosing intake and exhaust system for engine is verified. The carbon dioxide trapping device is designed and the IP52FMI engine is transformed and the CO2 capture experiment is carried out. The experimental results show that when the EGR rate is 36% for the optimum EGR rate. When the liquid oxygen of 35.80-437.40 g is imported into the device and last 1-20 min, respectively, 21.50-701.30 g dry ice is obtained. This research proposes a new design method which can capture CO2 for vehicular internal combustion engine. 展开更多
关键词 internal combustion Rankle cycle engine CO2 emission reduction carbon-sequestration with liquid oxygen KIVA-3V program oxygen-enriched combustion numerical simulation experiments
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A diagnostic experiment of the influence of CO_2on winter wheat 被引量:1
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作者 Wang Chunyi Bai Yueming Wen Min(Chinese Acaderny of Meteorological Sciences , Beijing 100081 , China) 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 1995年第2期167-175,共9页
AdiagnosticexperimentoftheinfluenceofCO_2onwinterwheatWangChunyi;BaiYueming;WenMin(ChineseAcadernyofMeteorol... AdiagnosticexperimentoftheinfluenceofCO_2onwinterwheatWangChunyi;BaiYueming;WenMin(ChineseAcadernyofMeteorologicalSciences,Be... 展开更多
关键词 winter wheat CO_2 OTC -1 open top chamber ventilated experiment.
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Overview of HL-2A Experiment Results 被引量:1
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作者 YANG Qingwei LIU Yong DING Xuantong YAN Longwen LIU Dequan XUAN Weiming CHEN Liaoyuan RAO Jun SONG Xianming CAO Zeng ZHANG Jinhua DUAN Xuru MAO Weicheng ZHOU Caipin PAN Chuanhong 《Southwestern Institute of Physics Annual Report》 2006年第1期3-11,共9页
The recent experiment results of the HL-2A tokamak are presented in this paper. Up to now, the stable and reproducible discharges with divertor configuration have been obtained under the main plasma parameters of Ip=4... The recent experiment results of the HL-2A tokamak are presented in this paper. Up to now, the stable and reproducible discharges with divertor configuration have been obtained under the main plasma parameters of Ip=433 kA, BT=2.70 T, ne-6.0×10^19 m^-3 and the discharge duration -3.0 s, using the reliable feedback control. Siliconization is the normal wall conditioning method on HL-2A. The impurity concentration and radiated power can be obviously suppressed after siliconization. Advanced scenario with weak positive/negative current shear, calculated by TRANSP codes using experimental data, is achieved by the central fuelling of the pellet injection. The supersonic molecular beam injection (SMBI) with liquid nitrogen temperature gas is used. It is proved that the low temperature SMBI can form the hydrogen cluster and penetrate into plasma more deeply and efficiently. The particle diffusion coefficient is obtained as 0.5 - 1.5 m^2/s at plasma peripheral area. The symmetries (m = 0- 1, n = 0) of the directly measured low frequency (7 -9 kHz ) electric potential and field are simultaneously observed for the first time by a novel designed Langmuir probe system. The impurity diffusion coefficient and convection velocity are determined by using laser blow-off system and transport code. In disruption studies, a new criterion for disruption prediction is given according to the MHD activity features and statistic analysis, and the plasma current quench time increases from about 5 ms to 20 ms with noble gas injection. The sawtooth character in on-axis ECRH experiment is investigated, and a persistent m= 1 oscillation induced by SMBI and pellet injection is found to be a thermal fluctuation instead of magnetic island. The detachment characteristics of HL-2A divertor are numerically and experimentally studied using code SOLPS5.0 and measured data. The long divertor legs and thin divertor throats in HL-2A pose MHD shaping problems resulting in momentum losses even at low densities and strongly enhanced main chamber losses. 展开更多
关键词 experiment results HL-2A
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Responses of Planetary Waves to Global Warming: Implications from CMIP3 4×CO_2 Experiments
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作者 WEN Xin-Yu JIA Zhe TAN Ben-Kui 《Atmospheric and Oceanic Science Letters》 CSCD 2013年第2期109-116,共8页
The planetary wave response to global warm ing with single forcing of greenhouse gases (GHGs) is investigated in this study by using a total of 11 model results that anticipated CMIP3 4XCO2 experiments. It is shown ... The planetary wave response to global warm ing with single forcing of greenhouse gases (GHGs) is investigated in this study by using a total of 11 model results that anticipated CMIP3 4XCO2 experiments. It is shown that the amplitudes of the planetary wave fluxes over Siberia, the Eastern North Pacific, and the North Atlantic decrease by approximately -10% to -30% in the warming context. In particular, the vertical wave flux over the Eastern North Pacific significantly decreases by -28.6%. The weakening of the planetary waves is partly associated with the decreased land-sea thermal contrast, which may be caused by the radiation effect of CO2 and the different surface heat capacities of land and sea. The present work provides a clear understanding of the re sponses of planetary waves to GHGs forcing. 展开更多
关键词 planetary wave stationary-wave globalwarming 4~CO2 experiments
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Experimental and numerical studies of Ca(OH)_(2)/CaO dehydration process in a fixed-bed reactor for thermochemical energy storage
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作者 Zhihao Zhang Danyang Song +2 位作者 Hengxing Bao Xiang Ling Xiaogang Jin 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2023年第10期11-20,共10页
The Ca(OH)_(2)/CaO thermochemical energy storage(TCES)system based on calcium looping has received extensive attention owing to its high energy storage density,prolonged energy storage time,and environmental friendlin... The Ca(OH)_(2)/CaO thermochemical energy storage(TCES)system based on calcium looping has received extensive attention owing to its high energy storage density,prolonged energy storage time,and environmental friendliness.The heat storage process of the Ca(OH)_(2)/CaO TCES system in a mixed heating reactor was evaluated in this study,by employing a combination of direct and indirect heating modes.The dehydration process was studied experimentally,and a numerical model was established and verified based on the experimental results.The dehydration behavior of 500 g of Ca(OH)_(2) powder was investigated in a fixed-bed reactor with mixed heating.The experimental and simulation results indicated that mixed heating causes combined centripetal and horizontal propulsion.Heat input is the main limiting factor in the heat storage process,because the radial advance of the reaction is hindered by the low thermal conductivity of the solid reactant particles.Heat transmission partitions were added to enhance the performance of the reactor.The performance of the modified reactor was compared with that of a conventional reactor.The radial heat transmission partitions in the modified reactor effectively enhance the energy storage rate and reduce the reaction time by 59.5%compared with the reactor without partitions. 展开更多
关键词 Thermochemical energy storage REACTOR Ca(OH)_(2)/CaO DEHYDRATION experiment research Numerical simulation
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Effects of CH_(4)/CO_(2) multi-component gas on components and properties of tight oil during CO_(2) utilization and storage: Physical experiment and composition numerical simulation
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作者 Zhi-Hao Jia Ren-Yi Cao +5 位作者 Bin-Yu Wang Lin-Song Cheng Jin-Chong Zhou Bao-Biao Pu Fu-Guo Yin Ming Ma 《Petroleum Science》 SCIE EI CAS CSCD 2023年第6期3478-3487,共10页
An essential technology of carbon capture, utilization and storage-enhanced oil recovery (CCUS-EOR) for tight oil reservoirs is CO_(2) huff-puff followed by associated produced gas reinjection. In this paper, the effe... An essential technology of carbon capture, utilization and storage-enhanced oil recovery (CCUS-EOR) for tight oil reservoirs is CO_(2) huff-puff followed by associated produced gas reinjection. In this paper, the effects of multi-component gas on the properties and components of tight oil are studied. First, the core displacement experiments using the CH_(4)/CO_(2) multi-component gas are conducted to determine the oil displacement efficiency under different CO_(2) and CH_(4) ratios. Then, a viscometer and a liquid density balance are used to investigate the change characteristics of oil viscosity and density after multi-component gas displacement with different CO_(2) and CH_(4) ratios. In addition, a laboratory scale numerical model is established to validate the experimental results. Finally, a composition model of multi-stage fractured horizontal well in tight oil reservoir considering nano-confinement effects is established to investigate the effects of multi-component gas on the components of produced dead oil and formation crude oil. The experimental results show that the oil displacement efficiency of multi-component gas displacement is greater than that of single-component gas displacement. The CH_(4) decreases the viscosity and density of light oil, while CO_(2) decreases the viscosity but increases the density. And the numerical simulation results show that CO_(2) extracts more heavy components from the liquid phase into the vapor phase, while CH_(4) extracts more light components from the liquid phase into the vapor phase during cyclic gas injection. The multi-component gas can extract both the light components and the heavy components from oil, and the balanced production of each component can be achieved by using multi-component gas huff-puff. 展开更多
关键词 Multi-component gas Properties and components Core displacement experiment Nano-confinement numerical simulation CO_(2)utilization and storage
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An Experimental Investigation into the Amalgamated Al2O3-40% TiO2 Atmospheric Plasma Spray Coating Process on EN24 Substrate and Parameter Optimization Using TLBO
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作者 Thankam Sreekumar Rajesh Ravipudi Venkata Rao 《Journal of Materials Science and Chemical Engineering》 2016年第6期51-65,共15页
Surface coating is a critical procedure in the case of maintenance engineering. Ceramic coating of the wear areas is of the best practice which substantially enhances the Mean Time between Failure (MTBF). EN24 is a co... Surface coating is a critical procedure in the case of maintenance engineering. Ceramic coating of the wear areas is of the best practice which substantially enhances the Mean Time between Failure (MTBF). EN24 is a commercial grade alloy which is used for various industrial applications like sleeves, nuts, bolts, shafts, etc. EN24 is having comparatively low corrosion resistance, and ceramic coating of the wear and corroding areas of such parts is a best followed practice which highly improves the frequent failures. The coating quality mainly depends on the coating thickness, surface roughness and coating hardness which finally decides the operability. This paper describes an experimental investigation to effectively optimize the Atmospheric Plasma Spray process input parameters of Al<sub>2</sub>O<sub>3</sub>-40% TiO<sub>2</sub> coatings to get the best quality of coating on EN24 alloy steel substrate. The experiments are conducted with an Orthogonal Array (OA) design of experiments (DoE). In the current experiment, critical input parameters are considered and some of the vital output parameters are monitored accordingly and separate mathematical models are generated using regression analysis. The Analytic Hierarchy Process (AHP) method is used to generate weights for the individual objective functions and based on that, a combined objective function is made. An advanced optimization method, Teaching-Learning-Based Optimization algorithm (TLBO), is practically utilized to the combined objective function to optimize the values of input parameters to get the best output parameters. Confirmation tests are also conducted and their output results are compared with predicted values obtained through mathematical models. The dominating effects of Al<sub>2</sub>O<sub>3</sub>-40% TiO<sub>2</sub> spray parameters on output parameters: surface roughness, coating thickness and coating hardness are discussed in detail. It is concluded that the input parameters variation directly affects the characteristics of output parameters and any number of input as well as output parameters can be easily optimized using the current approach. 展开更多
关键词 Atmospheric Plasma Spray (APS) EN24 Design of experiments (DOE) Teaching Learning Based Optimization (TLBO) Analytic Hierarchy Process (AHP) Al2O3-40% TiO2
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大气等离子喷涂NiCoCrAlYTa-Cr_(2)O_(3)-Cu-Mo高温耐磨涂层组织和性能
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作者 张昂 郭孟秋 +5 位作者 王长亮 张梅 岳震 王天颖 聂梓杏 高燊 《材料工程》 北大核心 2025年第1期202-210,共9页
为了探究工艺参数对NiCoCrAlYTa-Cr_(2)O_(3)-Cu-Mo高温耐磨涂层性能的影响规律,基于正交实验,采用大气等离子喷涂(atmospheric plasma spray,APS)工艺制备NiCoCrAlYTa-Cr_(2)O_(3)-Cu-Mo涂层,应用极差分析法研究工艺参数对NiCoCrAlYTa-... 为了探究工艺参数对NiCoCrAlYTa-Cr_(2)O_(3)-Cu-Mo高温耐磨涂层性能的影响规律,基于正交实验,采用大气等离子喷涂(atmospheric plasma spray,APS)工艺制备NiCoCrAlYTa-Cr_(2)O_(3)-Cu-Mo涂层,应用极差分析法研究工艺参数对NiCoCrAlYTa-Cr_(2)O_(3)-Cu-Mo涂层显微组织、硬度和结合强度性能影响的主次关系,完成喷涂工艺参数优化。优化后的工艺参数为氩气流量为50 L/min,氢气流量为12 L/min,电流为500 A,喷涂距离为100 mm。结果表明:采用优化后的工艺参数喷涂的NiCoCrAlYTa-Cr_(2)O_(3)-Cu-Mo涂层显微组织均匀致密,涂层孔隙率小于1%,结合强度平均值为70.7 MPa,硬度平均值为543.7HV,900℃温度下50~100 h平均氧化速率为0.07302 g/(m^(2)·h),达到完全抗氧化级别,在800℃表现出良好的摩擦磨损性能,平均摩擦因数为0.248,磨损率为2.12×10^(-6) mm^(3)/(N·m)。 展开更多
关键词 NiCoCrAlYTa-Cr_(2)O_(3)-Cu-Mo涂层 大气等离子喷涂 高温耐磨 正交实验 抗氧化 显微组织
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基于Co/SiO_(2)催化剂的制备、表征及在加氢反应中的应用的综合教学实验设计
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作者 孙道来 张鹤千 《广州化工》 2025年第2期205-207,共3页
绿色化学是一门从源头上防止污染的化学,是一种能最大限度从资源合理利用、环境保护及生态平衡等方面满足人类可持续发展的化学。因此,在实验教学中引入绿色化学的相关内容和理念,对培养学生的综合能力具有重要的意义。本论文在综合化... 绿色化学是一门从源头上防止污染的化学,是一种能最大限度从资源合理利用、环境保护及生态平衡等方面满足人类可持续发展的化学。因此,在实验教学中引入绿色化学的相关内容和理念,对培养学生的综合能力具有重要的意义。本论文在综合化学实验教学过程中,引入绿色化学的理念,设计了一个环境友好型的综合性实验:Co/SiO_(2)催化剂的制备、表征和在加氢反应中的应用。在该实验中,通过Co/SiO_(2)催化剂的合成、表征以及应用,使学生了解固体催化剂的基本制备方法和物理性质,以及催化剂在生物质由来的乙酰丙酸加氢制备绿色化学品过程中的应用方法。该实验能够同步强化学生的实验动手能力,激发学生对于绿色化学的学习兴趣,提升学生的自主能动性,产生了良好的教学效果。 展开更多
关键词 开放性实验 Co/SiO_(2)催化剂 催化转化 乙酰丙酸 绿色化学
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基于抑菌实验和网络药理学探讨D-柠檬烯、2-茨醇对白色念珠菌的抑制作用 被引量:1
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作者 童鑫 帅维维 +1 位作者 唐喆 唐燕燕 《中医药信息》 2024年第4期7-13,共7页
目的:采用抑菌实验研究蛇床子-冰片药对成分中的D-柠檬烯及2-茨醇的体外抗白色念珠菌作用,并运用网络药理学预测D-柠檬烯和2-茨醇治疗念珠菌病的核心靶点和通路。方法:以白色念珠菌为研究对象,K-B纸片扩散法分别测定0.5、1.0、1.5 mg的D... 目的:采用抑菌实验研究蛇床子-冰片药对成分中的D-柠檬烯及2-茨醇的体外抗白色念珠菌作用,并运用网络药理学预测D-柠檬烯和2-茨醇治疗念珠菌病的核心靶点和通路。方法:以白色念珠菌为研究对象,K-B纸片扩散法分别测定0.5、1.0、1.5 mg的D-柠檬烯、2-茨醇、制霉菌素的药液抑菌圈直径;采用试管双倍稀释法和棋盘法,测定D-柠檬烯、2-茨醇的最低抑菌浓度(MIC)以及两两联用的MIC,计算出联合抑菌分数(FIC)。通过Pubchem、SwissTargetPrediction数据库预测D-柠檬烯、2-茨醇的有效靶点;通过GeneCards、OMIM数据库检索念珠菌病相关的疾病靶点;运用Venny软件获得两种化学成分和念珠菌病的共同靶点;运用Cytoscape 3.9. 0软件构建“成分-靶点-疾病”网络;利用STRING数据库构建蛋白互作PPI网络;利用R软件进行GO功能及KEGG通路富集分析。结果:D-柠檬烯的MIC为5 mg/mL,2-茨醇的MIC为2.5 mg/mL。D-柠檬烯与2-茨醇联用的FIC指数为0.75,呈相加作用。网络药理学筛选得到两种成分相关作用靶点152个,疾病靶点893个,两者交集靶点为24个;网络拓扑分析得到核心靶点为肿瘤坏死因子(TNF)、过氧化物酶体增殖物激活受体γ(PPARG)、雌激素受体(ESR1)等;KEGG分析得到核心通路为C型凝集素受体信号通路(C-type lectin receptor signaling pathway)、Fc epsilon RI信号通路(Fc epsilon RI signaling pathway)、催乳素信号通路(prolactin signaling pathway)等。结论:D-柠檬烯、2-茨醇对白色念珠菌均有抑制作用,且2种组分药物联合使用具有一定的协同作用。网络药理学预测初步提示D-柠檬烯、2-茨醇可能通过作用于TNF、PPARG、ESR1等核心靶点调控C型凝集素受体信号通路(C-type lectin receptor signaling pathway)、Fc epsilon RI信号通路(Fc epsilon RI signaling pathway)等以治疗念珠菌病。 展开更多
关键词 白色念珠菌 D-柠檬烯 2-茨醇 抑菌实验 网络药理学
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茄衣新品系川雪2号全国多点试验研究
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作者 何正川 刘勇 +9 位作者 阳苇丽 韦树谷 杜卫民 王要君 肖洁 盛玉珍 尹宏博 何炼 魏建均 代顺冬 《安徽农业科学》 CAS 2024年第13期22-26,共5页
为雪茄烟茄衣新品系川雪2号的品种审定、推广提供科学依据。以川雪2号及其他8份雪茄烟品系为试验材料,2021年在全国9个主要雪茄烟产区、2022年在全国8个主要雪茄烟产区开展多点试验。结果表明,川雪2号田间整齐、长势强,大田生育期88 d... 为雪茄烟茄衣新品系川雪2号的品种审定、推广提供科学依据。以川雪2号及其他8份雪茄烟品系为试验材料,2021年在全国9个主要雪茄烟产区、2022年在全国8个主要雪茄烟产区开展多点试验。结果表明,川雪2号田间整齐、长势强,大田生育期88 d、免疫TMV、抗根黑腐病、综合抗病能力较强、田间自然发病率整体较轻;平均产量为1533.30 kg/hm^(2),平均产值为83234.70元/hm^(2),中上等烟比例为74.98%,产量、产值、中上等烟比例、评吸总分等在9个参试品系中排名靠前。川雪2号总体表现较好,可以进行一定规模的示范种植并开展相关配套栽培技术研究。 展开更多
关键词 茄衣 品系 川雪2 多点试验
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利用化学助剂强化CO_(2)埋存实验设计
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作者 王文东 王程伟 +3 位作者 任慈 孙庆豪 苏玉亮 李蕾 《实验技术与管理》 CAS 北大核心 2024年第1期130-135,共6页
结合储层CO_(2)埋存技术,自主搭建了地层温度压力条件下CO_(2)埋存实验装置,开展了多介质辅助CO_(2)埋存实验研究。研究结果表明,乙醇-KOH体系能够有效进行CO_(2)矿化埋存,其中96%乙醇+3 g KOH 500 mL溶液捕集CO_(2)能力最强,是最佳的CO... 结合储层CO_(2)埋存技术,自主搭建了地层温度压力条件下CO_(2)埋存实验装置,开展了多介质辅助CO_(2)埋存实验研究。研究结果表明,乙醇-KOH体系能够有效进行CO_(2)矿化埋存,其中96%乙醇+3 g KOH 500 mL溶液捕集CO_(2)能力最强,是最佳的CO_(2)矿化埋存溶液配比。经CO_(2)矿化埋存后,低渗透岩心孔隙度平均降低7.07%,孔隙度变化率与孔隙度呈正相关关系,渗透率平均降低16.01%。因此,96%乙醇+3 g KOH能够加速CO_(2)在储层中的CO_(2)沉淀过程,缩短CO_(2)在储层中的矿化埋存时间。该研究可重复性、准确性和可扩展性较强,能够激发学生自主设计实验的积极性及创新意识,培养学生的独立思考能力,有利于学生将理论知识与实际工程问题相结合,实现科研能力与创新能力的相互促进。 展开更多
关键词 低渗透油藏 乙醇+KOH+CO_(2) CO_(2)埋存 矿化埋存 创新性实验
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基于核磁共振测井T_(2)谱评价储层孔隙结构仿真实验设计
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作者 谢然红 王翔宇 +1 位作者 金国文 郭江峰 《实验科学与技术》 2024年第6期20-27,共8页
储层孔隙结构评价对油气资源勘探开发和二氧化碳地质封存等具有重要意义。将核磁共振测井横向弛豫时间(T_(2))谱转换为毛管压力曲线是进行储层孔隙结构评价的有效手段。针对转换模型理论性强、原理抽象复杂等特点,利用MATLAB GUI设计了... 储层孔隙结构评价对油气资源勘探开发和二氧化碳地质封存等具有重要意义。将核磁共振测井横向弛豫时间(T_(2))谱转换为毛管压力曲线是进行储层孔隙结构评价的有效手段。针对转换模型理论性强、原理抽象复杂等特点,利用MATLAB GUI设计了基于核磁共振测井T_(2)谱评价储层孔隙结构的仿真实验。该交互界面包含毛管压力曲线数据预处理、核磁共振T_(2)谱转换毛管压力曲线及孔隙结构特征参数提取等模块。通过在仿真实验中利用不同转换方法将核磁共振T_(2)谱转换为毛管压力曲线,并将处理结果与实验测量数据在图形界面进行比对,最终从转换所得毛管压力曲线中提取孔隙结构特征参数,进行储层孔隙结构评价。该仿真实验操作简单,能将不同方法的处理过程与结果直观展示给学生,增强学生对不同转换模型预测毛管压力曲线的理解与认识,提高了地球物理测井教学效果。 展开更多
关键词 孔隙结构评价 毛管压力曲线 核磁共振测井T_(2)谱 MATLAB仿真实验
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两种不同形貌纳米Cu_(2)(Salen)_(2)的制备与表征综合实验设计
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作者 苑娟 张彬 +1 位作者 吴金萍 王梦凡 《大学化学》 CAS 2024年第10期420-425,共6页
分别采用反溶剂沉淀法和溶剂热法制备了两种不同形貌的Cu_(2)(Salen)_(2)纳米材料,通过红外光谱(IR)、X射线粉末衍射(XRD)和扫描电子显微镜(SEM)等手段对其进行了结构和形貌表征,并利用Olex2软件对该配合物的分子结构和配位情况进行了... 分别采用反溶剂沉淀法和溶剂热法制备了两种不同形貌的Cu_(2)(Salen)_(2)纳米材料,通过红外光谱(IR)、X射线粉末衍射(XRD)和扫描电子显微镜(SEM)等手段对其进行了结构和形貌表征,并利用Olex2软件对该配合物的分子结构和配位情况进行了展示。本实验内容涉及有机合成、配位化学、纳米材料制备与表征、晶体结构分析软件应用等,将基础理论知识与现代科技手段有机统一,兼顾基础的同时,注重创新能力和综合素质的培养。本实验将前沿科研成果转化到实验教学实践中,使二者相互促进,形成良性循环。 展开更多
关键词 综合实验 Cu_(2)(Salen)_(2) 纳米材料 反溶剂沉淀法 溶剂热法 合成与表征
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VITEK■ 2 Compact全自动微生物鉴定药敏分析仪虚拟仿真教学软件的开发与应用
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作者 黄荔丰 《工业微生物》 CAS 2024年第3期31-34,共4页
文章主要介绍了VITEK■2 Compact全自动微生物鉴定药敏分析仪虚拟仿真教学软件的设计与开发,以及虚拟仿真融合线上线下混合教学模式在医学微生物检验实验教学中的应用。结果表明,该仿真教学软件的实际应用既有利于培养学生的实践操作能... 文章主要介绍了VITEK■2 Compact全自动微生物鉴定药敏分析仪虚拟仿真教学软件的设计与开发,以及虚拟仿真融合线上线下混合教学模式在医学微生物检验实验教学中的应用。结果表明,该仿真教学软件的实际应用既有利于培养学生的实践操作能力及思维方式,又能弥补学校实训环境与临床实际不符的缺陷,为微生物检验实验教学质量的提升开辟新的思路。 展开更多
关键词 VITEK■2 Compact 虚拟仿真 微生物学检验 实验教学
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