Fragments of nuclear ribosomal 28S rDNA D2 divergent domain, mitochondrial 16S rDNA, and COI partial genes of 15 species in the subgenus Singhardina Mahmood from China were amplified and sequenced. Molecular phylogene...Fragments of nuclear ribosomal 28S rDNA D2 divergent domain, mitochondrial 16S rDNA, and COI partial genes of 15 species in the subgenus Singhardina Mahmood from China were amplified and sequenced. Molecular phylogenetic trees were constructed using maximum parsimony, maximum likelihood and Bayesian inference methods. Results from these methods revealed similar topologies with recognizable relationships among subclades. The phylogenetic relationship of four species groups of subgenus Singhardina Mahmood from China is discussed for the first time. The results show that Singhardina Mahmood forms a single lineage representing a monophyletic group. The Eurhadina （Singhardina） rubra species group newly proposed in this study is likely the most basal species group within Singhardina Mahmood. The E. （Singhardina） robusta species group is the sister group of the E. （Singhardina） mamata species group. Molecular evidence supports including the E. （Singhardina） vittata species group in the E. （Singhardina） punjabensis species group.展开更多
Egyptian species of the leafhopper genus Exitianus Ball,1929,E.capicola(St?l,1855),E.nanus(Distant,1908),and E.pondus Ross,1968 are reviewed.Illustrations,morphological descriptions,and a key for their identification ...Egyptian species of the leafhopper genus Exitianus Ball,1929,E.capicola(St?l,1855),E.nanus(Distant,1908),and E.pondus Ross,1968 are reviewed.Illustrations,morphological descriptions,and a key for their identification are provided.In this study,we used molecular techniques to confirm morphological identification and detect phylogeny among the three Exitianus species.The partial nucleotide sequences of the amplified products obtained were determined by Macrogen Korea.The nucleotide sequences of 28S rDNA and COX genes of the EGY-ARC-9,EGY-ARC-4,and EGY-ARC-5 were determined by Macrogen Korea,blasted into BLAST at the National Center for Biotechnology Information website(NCBI)and compared with those deposited in the GenBank DNA database.The results represented the homology percentage between the partial sequences of the 28S rRNA and COX genes from each species and related species obtained from GenBank DNA database.The morphological identifications of the three Exitianus species were confirmed by molecular characterization and sequencing of 28S rDNA and COX genes and identified as E.capicola,E.nanus,and E.pondus.Also,their sequences of 28srDNA and COX genes were deposited in GenBank with an accession number(LC670610,LC670607,and OQ196105)for 28srDNA gene and(LC775357,LC775358,and LC775359)for the COX gene.展开更多
Despite the diversity and ecological importance of cestodes,there is a paucity of studies on their life stages(i.e.,complete lists of intermediate,paratenic,and definitive hosts)and genetic variation.For example,in th...Despite the diversity and ecological importance of cestodes,there is a paucity of studies on their life stages(i.e.,complete lists of intermediate,paratenic,and definitive hosts)and genetic variation.For example,in the Gulf of Mexico(GoM)98 species of cestodes have been reported to date;however,data on their intraspecific genetic variation and population genetic studies are lacking.The trypanorhynch cestode,Oncomegas wageneri,is found(among other places)off the American Western Atlantic Coast,including the GoM,and has been reported as an adult from stingrays and from several teleost species in its larval form(as plerocerci).This study represents the first report of 2 previously unregistered definitive hosts for O.wageneri,namely the Atlantic sharpnose shark Rhizoprionodon terraenovae and the southern stingray Hypanus americanus.In this work,partial sequences of the 28S(region D1-D2)ribosomal DNA were analyzed to include O.wageneri within an eutetrarhynchoid phylogenetic framework.All O.wageneri individuals(which included plerocerci and adults)were recovered as monophyletic and Oncomegas celatus was identified as the sister species of O.wageneri.Furthermore,population genetic analyses of O.wageneri from the southern GoM were carried out using DNA sequences of the mitochondrial cytochrome c oxi-dase subunit 1(COI)gene,which reflected high genetic variation and a lack of genetic structure among the 9 oceanographic sampling sites.Based on these results,O.wageneri is panmictic in the southern GoM.More extensive sampling along the species entire distribution is necessary to make more accurate inferences of population genetics of O.wageneri.展开更多
基金supported by"The National Natural Science Foundation of China" (No. 30770262)"The Ministry of Science and Technology of the People's Republic of China " (No. 2006FY120100)partly by "Northwest A&F University Grants for Outstanding Young Faculty Members" (No.Z111020905)
文摘Fragments of nuclear ribosomal 28S rDNA D2 divergent domain, mitochondrial 16S rDNA, and COI partial genes of 15 species in the subgenus Singhardina Mahmood from China were amplified and sequenced. Molecular phylogenetic trees were constructed using maximum parsimony, maximum likelihood and Bayesian inference methods. Results from these methods revealed similar topologies with recognizable relationships among subclades. The phylogenetic relationship of four species groups of subgenus Singhardina Mahmood from China is discussed for the first time. The results show that Singhardina Mahmood forms a single lineage representing a monophyletic group. The Eurhadina （Singhardina） rubra species group newly proposed in this study is likely the most basal species group within Singhardina Mahmood. The E. （Singhardina） robusta species group is the sister group of the E. （Singhardina） mamata species group. Molecular evidence supports including the E. （Singhardina） vittata species group in the E. （Singhardina） punjabensis species group.
文摘Egyptian species of the leafhopper genus Exitianus Ball,1929,E.capicola(St?l,1855),E.nanus(Distant,1908),and E.pondus Ross,1968 are reviewed.Illustrations,morphological descriptions,and a key for their identification are provided.In this study,we used molecular techniques to confirm morphological identification and detect phylogeny among the three Exitianus species.The partial nucleotide sequences of the amplified products obtained were determined by Macrogen Korea.The nucleotide sequences of 28S rDNA and COX genes of the EGY-ARC-9,EGY-ARC-4,and EGY-ARC-5 were determined by Macrogen Korea,blasted into BLAST at the National Center for Biotechnology Information website(NCBI)and compared with those deposited in the GenBank DNA database.The results represented the homology percentage between the partial sequences of the 28S rRNA and COX genes from each species and related species obtained from GenBank DNA database.The morphological identifications of the three Exitianus species were confirmed by molecular characterization and sequencing of 28S rDNA and COX genes and identified as E.capicola,E.nanus,and E.pondus.Also,their sequences of 28srDNA and COX genes were deposited in GenBank with an accession number(LC670610,LC670607,and OQ196105)for 28srDNA gene and(LC775357,LC775358,and LC775359)for the COX gene.
基金Grant No.Al-S-15134 by the Conscjo National de Ciencia y Tecnologia(CONACyT)(to F.S.C.)Research funded by the CONACyT(Mexican Ministry of Energy)Hydrocarbon Trust,project 201441.
文摘Despite the diversity and ecological importance of cestodes,there is a paucity of studies on their life stages(i.e.,complete lists of intermediate,paratenic,and definitive hosts)and genetic variation.For example,in the Gulf of Mexico(GoM)98 species of cestodes have been reported to date;however,data on their intraspecific genetic variation and population genetic studies are lacking.The trypanorhynch cestode,Oncomegas wageneri,is found(among other places)off the American Western Atlantic Coast,including the GoM,and has been reported as an adult from stingrays and from several teleost species in its larval form(as plerocerci).This study represents the first report of 2 previously unregistered definitive hosts for O.wageneri,namely the Atlantic sharpnose shark Rhizoprionodon terraenovae and the southern stingray Hypanus americanus.In this work,partial sequences of the 28S(region D1-D2)ribosomal DNA were analyzed to include O.wageneri within an eutetrarhynchoid phylogenetic framework.All O.wageneri individuals(which included plerocerci and adults)were recovered as monophyletic and Oncomegas celatus was identified as the sister species of O.wageneri.Furthermore,population genetic analyses of O.wageneri from the southern GoM were carried out using DNA sequences of the mitochondrial cytochrome c oxi-dase subunit 1(COI)gene,which reflected high genetic variation and a lack of genetic structure among the 9 oceanographic sampling sites.Based on these results,O.wageneri is panmictic in the southern GoM.More extensive sampling along the species entire distribution is necessary to make more accurate inferences of population genetics of O.wageneri.
