The synthetic route of 3’-amino carboxylate hydrochloride of quercetin is improved.Using benzyl bromide to partly protect the hydroxyl groups,the protected quercetin then react with a series of Boc protected amino ac...The synthetic route of 3’-amino carboxylate hydrochloride of quercetin is improved.Using benzyl bromide to partly protect the hydroxyl groups,the protected quercetin then react with a series of Boc protected amino acids to afford the 3’-amino carboxylate,hydrogenised and treated with HCl(g),a series of 3’-amino carboxylate hydrochloride of quercetin are synthesized in good yields.The structures of these compounds are confirmed by means of their m.p.,mass spectra and 1H NMR.展开更多
目的探讨阿霉素诱导胃癌细胞磷脂酰肌醇3’-激酶(P I3’K)/A k t/FKHRL 1通路的激活对胃癌细胞SGC-7901化疗效果的影响及二者的关系。方法阿霉素及P I3’K/A k t抑制剂W ortm ann in分别作用于胃癌细胞SGC-7901,M TT比色法检测胃癌细胞...目的探讨阿霉素诱导胃癌细胞磷脂酰肌醇3’-激酶(P I3’K)/A k t/FKHRL 1通路的激活对胃癌细胞SGC-7901化疗效果的影响及二者的关系。方法阿霉素及P I3’K/A k t抑制剂W ortm ann in分别作用于胃癌细胞SGC-7901,M TT比色法检测胃癌细胞的生存率,W estern印迹法检测FKHRL 1磷酸化表达水平。结果阿霉素抑制胃癌细胞SGC-7901的生长,呈时间依赖性诱导FKHRL 1磷酸化。W ortm ann in可明显增强阿霉素的细胞生长抑制作用,同时下调阿霉素诱导的磷酸化FKHRL 1表达。结论阿霉素可能通过激活SGC-7901细胞的P I3’K/A k t通路诱导FKHRL 1磷酸化,从而影响胃癌细胞的化疗耐药性。W ortm ann in可以阻断P I3’K/A k t/FKHRL 1通路而提高胃癌的化疗敏感性。展开更多
文摘The synthetic route of 3’-amino carboxylate hydrochloride of quercetin is improved.Using benzyl bromide to partly protect the hydroxyl groups,the protected quercetin then react with a series of Boc protected amino acids to afford the 3’-amino carboxylate,hydrogenised and treated with HCl(g),a series of 3’-amino carboxylate hydrochloride of quercetin are synthesized in good yields.The structures of these compounds are confirmed by means of their m.p.,mass spectra and 1H NMR.
文摘目的探讨阿霉素诱导胃癌细胞磷脂酰肌醇3’-激酶(P I3’K)/A k t/FKHRL 1通路的激活对胃癌细胞SGC-7901化疗效果的影响及二者的关系。方法阿霉素及P I3’K/A k t抑制剂W ortm ann in分别作用于胃癌细胞SGC-7901,M TT比色法检测胃癌细胞的生存率,W estern印迹法检测FKHRL 1磷酸化表达水平。结果阿霉素抑制胃癌细胞SGC-7901的生长,呈时间依赖性诱导FKHRL 1磷酸化。W ortm ann in可明显增强阿霉素的细胞生长抑制作用,同时下调阿霉素诱导的磷酸化FKHRL 1表达。结论阿霉素可能通过激活SGC-7901细胞的P I3’K/A k t通路诱导FKHRL 1磷酸化,从而影响胃癌细胞的化疗耐药性。W ortm ann in可以阻断P I3’K/A k t/FKHRL 1通路而提高胃癌的化疗敏感性。
