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Role of 3'-untranslated region translational control in cancer development, diagnostics and treatment 被引量:6
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作者 Andrii Vislovukh Thaiz Rivera Vargas +1 位作者 Anna Polesskaya Irina Groisman 《World Journal of Biological Chemistry》 CAS 2014年第1期40-57,共18页
The messenger RNA 3'-untranslated region(3'UTR)plays an important role in regulation of gene expres-sion on the posttranscriptional level. The 3'UTR con-trols gene expression via orchestrated interactionbe... The messenger RNA 3'-untranslated region(3'UTR)plays an important role in regulation of gene expres-sion on the posttranscriptional level. The 3'UTR con-trols gene expression via orchestrated interactionbetween the structural components of mRNAs(cis-ele-ment) and the specific trans-acting factors(RNA bind-ing proteins and non-coding RNAs). The crosstalk ofthese factors is based on the binding sequences and/or direct protein-protein interaction, or just functionalinteraction. Much new evidence that has accumulatedsupports the idea that several RNA binding factors canbind to common mRNA targets: to the non-overlappingbinding sites or to common sites in a competitive fash-ion. Various factors capable of binding to the sameRNA can cooperate or be antagonistic in their actions.The outcome of the collective function of all factorsbound to the same mRNA 3'UTR depends on manycircumstances, such as their expression levels, affinity to the binding sites, and localization in the cell, which can be controlled by various physiological conditions. Moreover, the functional and/or physical interactions of the factors binding to 3'UTR can change the character of their actions. These interactions vary during the cell cycle and in response to changing physiological condi-tions. Abnormal functioning of the factors can lead to disease. In this review we will discuss how alterations of these factors or their interaction can affect cancer development and promote or enhance the malignant phenotype of cancer cells. Understanding these altera-tions and their impact on 3'UTR-directed posttran-scriptional gene regulation will uncover promising new targets for therapeutic intervention and diagnostics. We will also discuss emerging new tools in cancer di-agnostics and therapy based on 3'UTR binding factors and approaches to improve them. 展开更多
关键词 Translational control 3 -untranslated region MICRORNAS RNA binding proteins CANCER
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Evolutionary relationship of 5′-untranslated regions among Thai dengue-3 viruses,Bangkok isolates,during 24 year-evolution
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作者 Watcharee Attatippaholkun Panyupa Pankhong +1 位作者 Ananda Nisalak Siripen Kalayanarooj 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2015年第3期176-184,共9页
Objective:To study evolutionary relationship of the 5'untranslated regions(5'UTRs) in low passage dengue3 viruses(DEN3) isolated from hospitalized children with different clinical manifestations in Bangkok dur... Objective:To study evolutionary relationship of the 5'untranslated regions(5'UTRs) in low passage dengue3 viruses(DEN3) isolated from hospitalized children with different clinical manifestations in Bangkok during 24 year-evolution(1977-2000) comparing to the DEN3prototype(H87).Methods:The 5'UTRs of these Thai DEN3 and the H87 prototype were amplified by RT-PCR and sequenced.Their multiple sequence alignments were done by Codon Code Aligner v 4.0.4 software and their RNA secondary structures were predicted by MFOLD software.Replication of five Thai DEN3 candidates comparing to the 1187 prototype were done in human(HepG2) and the mosquito(C6/36) cell lines.Results:Among these Thai DEN3,the completely identical sequences of their first 89 nucleotides,their high-order secondary structure of 5'UTRs and three SNPs including the predominant C90 T,and two minor SNPs including A109 G and A112 G were found.The C90 T of Thai DEN3.Bangkok isolates was shown predominantly before 1977.Five Thai DEN3 candidates with the predominant C90 T were shown to replicate in human(HepG2) and the mosquito(C6/36) cell lines better than the H87 prototype.However,their highly conserved sequences as well as SNPs of the 5'UTR did not appear to correlate with their disease severity in human.Conclusions:Our findings highlighted evolutionary relationship of the completely identical 89 nucleotide sequence,the high-order secondary structure and the predominant C90 T of the 5'UTR of these Thai DEN3 during 24 year-evolution further suggesting to be their genetic markers and magic targets for future research on antiviral therapy as well as vaccine approaches of Thai DEN3. 展开更多
关键词 THAI dengue-3 viruses Evolutionary relationship 5’untranslated regions 24 Year-evolution
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TYMS gene 5'-and 3'-untranslated region polymorphisms and risk of non-syndromic cleft lip and palate in an Indian population
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作者 Jyotsna Murthy Venkatesh Babu G. L.V.K.S.Bhaskar 《The Journal of Biomedical Research》 CAS CSCD 2015年第4期337-339,共3页
Dear Editor: Increased homocysteine levels due to vitamin B6 or B12 deficiency or genetic defects in folate pathway genes are associated with an increased incidence of non-syndromic cleft lip with or without cleft p... Dear Editor: Increased homocysteine levels due to vitamin B6 or B12 deficiency or genetic defects in folate pathway genes are associated with an increased incidence of non-syndromic cleft lip with or without cleft palate (NSCLP)tlj. Thymidylate synthase (TS) is a folate-dependent enzyme that catalyzes methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deox- ythymidine-5'-monophosphate (dTMP), a rate-limiting step in DNA synthesis, 展开更多
关键词 TYMS gene 5 untranslated region polymorphisms and risk of non-syndromic cleft lip and palate in an Indian population and 3 GENE
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0~3岁婴幼儿托育教养人员生存状态的地区差异及影响因素分析
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作者 秦旭芳 臧天品 黄思芸 《重庆第二师范学院学报》 2024年第4期74-81,128,共9页
关注0~3岁婴幼儿托育教养人员的生存状态有利于其专业成长和队伍稳定。通过对东北、华东、西部三个地区666名托育教养人员生存状态的实证调查,发现托育教养人员生存状态总体水平中等偏上且存在地区差异,政府保障是影响东北地区托育教养... 关注0~3岁婴幼儿托育教养人员的生存状态有利于其专业成长和队伍稳定。通过对东北、华东、西部三个地区666名托育教养人员生存状态的实证调查,发现托育教养人员生存状态总体水平中等偏上且存在地区差异,政府保障是影响东北地区托育教养人员生存状态的关键因素,机构管理是影响华东地区托育教养人员生存状态的潜在因素,个体主观是影响西部地区托育教养人员生存状态的驱动因素。基于调查结果,提出改善托育教养人员生存状态的建议。 展开更多
关键词 0~3岁婴幼儿 托育教养人员 生存状态 地区差异
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血清金属蛋白酶组织抑制剂3和性别决定区Y框蛋白2在2型糖尿病肾损伤早期诊断中的临床应用
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作者 张洪利 王海成 +2 位作者 李华 未瑞超 曾玲 《临床肾脏病杂志》 2024年第7期529-535,共7页
目的探究血清金属蛋白酶组织抑制剂3(tissue inhibitors of metalloproteinases 3,TIMP3)和性别决定区Y框蛋白2(transcription factor determining region Y box protein 2,SOX2)对2型糖尿病(type 2 diabetes mellitus,T2DM)肾损伤的早... 目的探究血清金属蛋白酶组织抑制剂3(tissue inhibitors of metalloproteinases 3,TIMP3)和性别决定区Y框蛋白2(transcription factor determining region Y box protein 2,SOX2)对2型糖尿病(type 2 diabetes mellitus,T2DM)肾损伤的早期诊断。方法选取2022年4月至2023年4月在华北石油管理局总医院就诊的102例T2DM患者为研究对象,根据24 h尿蛋白排泄率(uri-nary albumin excretion rate,UAER)将T2DM患者分为肾损伤组(n=40)和无肾损伤组(n=62),另选取同期在华北石油管理局总医院行体检的健康者50名为对照组。酶联免疫吸附法测定所有受试者血清TIMP3、SOX2水平;比较3组受试者一般资料、糖化血红蛋白(hemoglobin A1c,HbA1c)、UAER、血肌酐(serum creatinine,Scr)、估算肾小球滤过率(estimated glomerular filtration rate,eGFR)、血清TIMP3、SOX2水平;Pearson相关性分析血清TIMP3与SOX2及两者与HbA1c、UAER、Scr、eGFR之间的关系;受试者工作特征曲线(receiver operating characteristic curve,ROC)分析血清TIMP3、SOX2对T2DM患者肾损伤的诊断价值。结果T2DM患者血清TIMP3[(0.68±0.17)μg/L比(1.35±0.35)μg/L]及eGFR[(105.99±20.56)mL·min^(-1)·(1.73 m^(2))比(133.15±26.18)mL·min^(-1)·(1.73 m^(2))^(-1)]显著低于对照组(P<0.05),且肾损伤患者血清TIMP3[(0.47±0.11)μg/L比(0.82±0.21)^(-1)μg/L]及eGFR[(74.69±10.22)mL·min^(-1)·(1.73 m^(2))比(126.18±27.23)mL·min^(-1)·(1.73 m^(2))^(-1)]显著低于无肾损伤患者(P<0.05);血清SOX2[(8.91±1.82)kU/L比(5.15±1.31)kU/L]及HbA1c[(8.80±1.55)%比(5.52±0.83)%]、UAER[(70.13±18.06)mg/24 h比(13.22±3.61)mg/24 h]、Scr[(82.14±15.23)µmol/L比(53.19±5.62)µmol/L]显著高于对照组(P<0.05),且肾损伤患者血清SOX2[(10.81±2.13)kU/L比(5.15±1.31)kU/L]及UAER[(156.83±40.29)mg/24 h比(13.22±3.61)mg/24 h]、Scr[(113.77±13.58)µmol/L比(53.19±5.62)µmol/L]显著高于无肾损伤患者(P<0.05)。Pearson相关性分析结果显示,TIMP3与UAER、Scr、HbA1c呈显著负相关(P<0.05),与eGFR呈显著正相关(P<0.05);SOX2与UAER、Scr、HbA1c呈显著正相关(P<0.05),与eGFR呈显著负相关(P<0.05);血清TIMP3与SOX2呈显著负相关(r=-0.590,P<0.05)。ROC结果显示,血清TIMP3联合SOX2诊断T2DM患者肾损伤的敏感度和特异度分别为95.0%、85.3%,显著高于TIMP3、SOX2单独测定。结论T2DM肾损伤患者血清TIMP3水平显著降低,SOX2水平显著升高,且两者与T2DM患者肾损伤密切相关,可用于T2DM患者肾损伤早期诊断。 展开更多
关键词 金属蛋白酶组织抑制剂3 性别决定区框蛋白2 2型糖尿病 肾损伤 早期诊断
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Histone H3K27me3 methylation regulates the expression of secreted proteins distributed at fast-evolving regions through transcriptional repression of transposable elements 被引量:1
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作者 XIE Jia-hui TANG Wei +4 位作者 LU Guo-dong HONG Yong-he ZHONG Zhen-hui WANG Zong-hua ZHENG Hua-kun 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2023年第10期3059-3068,共10页
The fine-tuned expression dynamics of the effector genes are pivotal for the transition from vegetative growth to host colonization of pathogenic filamentous fungi.However,mechanisms underlying the dynamic regulation ... The fine-tuned expression dynamics of the effector genes are pivotal for the transition from vegetative growth to host colonization of pathogenic filamentous fungi.However,mechanisms underlying the dynamic regulation of these genes remain largely unknown.Here,through comparative transcriptome and chromatin immunoprecipitation sequencing(ChIP-seq)analyses of the methyltransferase PoKmt6 in rice blast fungus Pyricularia oryzae(syn.Magnaporthe oryzae),we found that PoKmt6-mediated H3K27me3 deposition was enriched mainly at fast-evolving regions and contributed to the silencing of a subset of secreted proteins(SP)and transposable element(TE)families during the vegetative growth of P.oryzae.Intriguingly,we observed that a group of SP genes,which were depleted of H3K27me3 modification,could also be silenced via the H3K27me3-mediated repression of the nearby TEs.In conclusion,our results indicate that H3K27me3 modification mediated by PoKmt6 regulates the expression of some SP genes in fast-evolving regions through the suppression of nearby TEs. 展开更多
关键词 secreted protein transposable elements fast-evolving regions H3K27me3
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狭缝引导配体13′UTR通过miR-34a-5p/SIRT1轴调节内皮细胞表型
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作者 欧涛 胡志琴 +5 位作者 高原 伍华燕 陈凯茵 徐金东 方咸宏 单志新 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2024年第3期362-372,共11页
在高等生物的进化过程中,mRNA的3′非翻译区(3′untranslated region,3′UTR)序列显著增加,提示3′UTR在生物功能调节中可能发挥重要作用。我们发现狭缝引导配体1(slit guidance ligand 1,SLIT1)3′UTR在肥厚型心肌病(HCM)患者心肌中水... 在高等生物的进化过程中,mRNA的3′非翻译区(3′untranslated region,3′UTR)序列显著增加,提示3′UTR在生物功能调节中可能发挥重要作用。我们发现狭缝引导配体1(slit guidance ligand 1,SLIT1)3′UTR在肥厚型心肌病(HCM)患者心肌中水平降低,但其对肥厚心肌中血管功能调节的作用机制不清楚。利用腺病毒介导在主动脉内皮细胞(human aortic endothelial cells,HAECs)中过表达SLIT13′UTR,检测HAECs中一氧化氮合酶(endothelial nitric oxide synthases,eNOS)和血管内皮生长因子A(vascular endothelial growth factor,VEGFA)表达。分别采用划痕实验和基于Matrigel胶的管腔形成实验检测HAECs的迁移和成管腔能力。结果发现,过表达SLIT13′UTR会升高HAECs中p-eNOS、eNOS和VEGFA水平(P<0.01),并促进HAECs迁移和成管腔能力(P<0.01)。生物信息学预测提示,SLIT13′UTR上有多个微RNA(miRNA)的潜在结合位点,反义RNA纯化技术(RNA antisense purification,RAP)筛选和利用Ago2抗体进行RNA结合蛋白质免疫沉淀(RNA binding protein immunoprecipitation,RIP)结果显示,SLIT13′UTR与miR-34a-5p存在结合作用,而过表达SLIT13′UTR的HAECs中miR-34a-5p水平显著降低(P<0.05)。Western印迹结果证实,miR-34a-5p可逆转过表达SLIT13′UTR对去乙酰化酶SIRT1的上调作用(P<0.05)。在HAECs中转染miR-34a-5p和si-SIRT1,可一致地抑制过表达SLIT13′UTR引起的p-eNOS、eNOS和VEGFA增加(P<0.05,P<0.01),及促进HAECs迁移和成管腔的能力(P<0.01,P<0.001)。因此,SLIT13′UTR可以特异性吸附miR-34a-5p,通过miR-34a-5p/SIRT1轴发挥促进内皮细胞迁移和管腔生成的作用。 展开更多
关键词 狭缝引导配体1 3′非翻译区 血管内皮细胞 微RNA
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邯郸地区2017—2021年PM_(2.5)和O_(3)污染特征
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作者 牛红亚 金妞 +4 位作者 胡塔峰 胡伟 王硕 史沥介 王金喜 《中国环境监测》 CAS CSCD 北大核心 2024年第3期60-70,共11页
利用实时监测数据分析2017—2021年邯郸市及周边区县PM_(2.5)和O_(3)污染特征。研究发现:2017—2021年各地区PM_(2.5)年均质量浓度持续降低,轻度及轻度以上污染逐渐减少;2017—2019年O_(3)污染加剧,2020年起O_(3)年均质量浓度逐年下降,... 利用实时监测数据分析2017—2021年邯郸市及周边区县PM_(2.5)和O_(3)污染特征。研究发现:2017—2021年各地区PM_(2.5)年均质量浓度持续降低,轻度及轻度以上污染逐渐减少;2017—2019年O_(3)污染加剧,2020年起O_(3)年均质量浓度逐年下降,污染天不断减少。PM_(2.5)和O_(3)-8 h分别在1月(平均浓度为127.3μg/m^(3),平均超标22d)和6月(平均浓度为233.4μg/m^(3),平均超标22 d)污染最严重。结合气象参数分析来看,PM_(2.5)与温度、风速和降水量呈显著负相关,与相对湿度呈显著正相关;O_(3)-8 h与温度呈显著正相关,而与风速、湿度和降水量的相关性较弱。后向轨迹和潜在源分析表明:邯郸地区PM_(2.5)典型污染月受山西省中部地区污染传输影响最大,O_(3)典型污染月受河南省东部污染传输影响最大。 展开更多
关键词 邯郸地区 PM_(2.5) O_(3) 污染特征
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3D Model Construction and Ecological Environment Investigation on a Regional Scale Using UAV Remote Sensing
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作者 Chao Chen Yankun Chen +7 位作者 Haohai Jin Li Chen Zhisong Liu Haozhe Sun Junchi Hong Haonan Wang Shiyu Fang Xin Zhang 《Intelligent Automation & Soft Computing》 SCIE 2023年第8期1655-1672,共18页
The acquisition of digital regional-scale information and ecological environmental data has high requirements for structural texture,spatial res-olution,and multiple parameter categories,which is challenging to achiev... The acquisition of digital regional-scale information and ecological environmental data has high requirements for structural texture,spatial res-olution,and multiple parameter categories,which is challenging to achieve using satellite remote sensing.Considering the convenient,facilitative,and flexible characteristics of UAV(unmanned air vehicle)remote sensing tech-nology,this study selects a campus as a typical research area and uses the Pegasus D2000 equipped with a D-MSPC2000 multi-spectral camera and a CAM3000 aerial camera to acquire oblique images and multi-spectral data.Using professional software,including Context Capture,ENVI,and ArcGIS,a 3D(three-dimensional)campus model,a digital orthophoto map,and multi-spectral remote sensing map drawing are realized,and the geometric accuracy of typical feature selection is evaluated.Based on a quantitative remote sensing model,the campus ecological environment assessment is performed from the perspectives of vegetation and water body.The results presented in this study could be of great significance to the scientific management and sustainable development of regional natural resources. 展开更多
关键词 Unmanned air vehicle multi-spectral camera oblique photography 3D model construction ecological environment investigation regional scale
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血清MR-ProADM、Gal-3在慢性心力衰竭患者中的表达及临床意义
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作者 王艳 韩军 周凡 《临床和实验医学杂志》 2024年第3期261-265,共5页
目的 观察血清肾上腺髓质前体中段肽(MR-ProADM)、半乳糖凝集素-3(Gal-3)在慢性心力衰竭(CHF)患者中的表达,并分析其临床意义。方法 前瞻性选取2020年7月至2021年6月在皖北煤电集团总医院治疗的80例CHF患者作为CHF组,再选取同期健康体... 目的 观察血清肾上腺髓质前体中段肽(MR-ProADM)、半乳糖凝集素-3(Gal-3)在慢性心力衰竭(CHF)患者中的表达,并分析其临床意义。方法 前瞻性选取2020年7月至2021年6月在皖北煤电集团总医院治疗的80例CHF患者作为CHF组,再选取同期健康体检的健康人群80名作为对照组。将其按纽约心脏协会(NYHA)分级再次分组为NYHAⅡ级组(n=27)、NYHAⅢ级组(n=32)、NYHAⅣ级组(n=21)。将CHF组患者随访12个月,按是否发生终点事件再次分组为不良预后组(n=26)和非不良预后组(n=54)。比较CHF组与对照组、各NYHA分组,规范抗心力衰竭治疗前后各NYHA分组、不良预后组和非不良预后组患者入组时的血清MR-ProADM、Gal-3水平,并采用受试者工作特征(ROC)曲线分析血清MR-ProADM、Gal-3水平对CHF患者不良预后的预测价值。结果 CHF组患者血清MR-ProADM、Gal-3水平分别为(702.69±47.16) pmol/mL、(26.11±5.78) ng/mL,均明显高于对照组[(587.21±40.69) pmol/mL、(2.34±0.69) ng/mL],差异均有统计学意义(P<0.05)。CHF组患者中,NYHAⅣ级组患者的血清MR-ProADM、Gal-3水平分别为(755.93±62.37) pmol/mL、(36.85±7.95) ng/mL,均明显高于Ⅲ级组[(690.61±49.26) pmol/mL、(20.21±4.07) ng/mL]和Ⅱ级组[(625.87±48.56) pmol/mL、(13.69±3.28) ng/mL],差异均有统计学意义(P<0.05)。CHF组各NYHA分组经治疗后的血清MR-ProADM、Gal-3水平均明显低于同组治疗前,差异均有统计学意义(P<0.05)。不良预后组患者入组时血清MR-ProADM、Gal-3水平分别为(711.26±47.22) pmol/mL、(29.87±5.39) ng/mL,均明显高于非不良预后组[(639.58±45.73) pmol/mL、(15.61±3.78) ng/mL],差异均有统计学意义(P<0.05)。血清MR-ProADM、Gal-3检测联合对CHF患者不良预后评估的曲线下面积(AUC)为0.893(95%CI:0.772~0.951,P<0.001),高于单独检测血清MR-ProADM预测[AUC为0.751(95%CI:0.619~0.892,P<0.001)]或血清Gal-3预测的AUC[0.855(95%CI:0.751~0.935,P<0.001)]。结论 血清MR-ProADM、Gal-3可作为CHF诊断、危险分层、治疗效果评估、预后预测的生物标志物。 展开更多
关键词 慢性心力衰竭 肾上腺髓质前体中段肽 半乳糖凝集素-3 临床意义
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渤海湾盆地石臼坨凸起秦皇岛27-3大型油田发现与地质新认识 被引量:6
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作者 周家雄 徐春强 +4 位作者 杨海风 张宏国 张震 柳永军 张德龙 《中国海上油气》 CAS CSCD 北大核心 2024年第1期14-25,共12页
渤海湾盆地石臼坨凸起历经50余年勘探,在凸起高部位背斜区发现了3个浅层亿吨级大油田,而低部位斜坡区浅层由于构造圈闭不发育,勘探程度较低,近10年无规模性发现。随着渤海海域勘探程度提高,现已全面进入岩性勘探阶段,针对石臼坨凸起东... 渤海湾盆地石臼坨凸起历经50余年勘探,在凸起高部位背斜区发现了3个浅层亿吨级大油田,而低部位斜坡区浅层由于构造圈闭不发育,勘探程度较低,近10年无规模性发现。随着渤海海域勘探程度提高,现已全面进入岩性勘探阶段,针对石臼坨凸起东段顺向断裂斜坡区浅层油气运移、岩性圈闭刻画和油气成藏规律等展开了深入系统研究。研究认为:(1)研究区南北两侧被富生烃凹陷夹持,凸起边界大断层晚期强烈活动和陡坡带近源扇体普遍发育使凸起区成为规模性油气运聚的最终指向区;(2)凸起边界大断层在馆陶组开启系数最小,馆陶组含砾砂岩输导层是石臼坨凸起之上主要的油气横向运移通道;(3)馆陶组输导脊坡度和切脊断层组合控制顺向斜坡区油气的垂向输导,当坡度小于1°且断层长度大于2.50 km、成藏期断距大于40 m时斜坡区可产生规模性的油气垂向运移,突破顺向断裂斜坡区“过路不留”的传统认识;(4)在凸起斜坡区,与构造脊方向斜交的砂体有利于油气保存,同时,提出井约束谱蓝化整形高分辨率处理技术,实现了对斜坡区岩性圈闭有效刻画,优选出保存条件较好的斜向砂体开展钻探部署。基于上述新认识、新技术,2023年在石臼坨凸起东段顺向斜坡区成功评价了秦皇岛27-3亿吨级油田,实现了勘探老区浅层挖潜的重大突破,为渤海湾盆地高成熟探区持续油气发现提供了借鉴。 展开更多
关键词 秦皇岛27-3 顺向斜坡区 油气运聚 岩性圈闭刻画 石臼坨凸起 渤海海域 浅层 油田发现
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基于免疫组库测序的肝硬化典型证候患者外周血T细胞受体β链CDR3多样性分析
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作者 王佳 胡宇婷 +3 位作者 龚跃峰 朱健 苏式兵 陆奕宇 《世界科学技术-中医药现代化》 CSCD 北大核心 2024年第7期1916-1924,共9页
目的 本研究通过免疫组库测序,分析肝硬化不同典型证候患者外周血TCR β链CDR3的多样性,探讨肝硬化证候的物质基础及其规律。方法 20例肝硬化患者为病例组,包括肝胆湿热(LGHD)、肝郁脾虚(LDSD)、肝肾阴虚(LKYD)3种证型,10例健康患者为... 目的 本研究通过免疫组库测序,分析肝硬化不同典型证候患者外周血TCR β链CDR3的多样性,探讨肝硬化证候的物质基础及其规律。方法 20例肝硬化患者为病例组,包括肝胆湿热(LGHD)、肝郁脾虚(LDSD)、肝肾阴虚(LKYD)3种证型,10例健康患者为正常对照组。从外周血样品中提取DNA,对TCRβ链CDR3进行多重PCR扩增,然后对产物进行高通量测序,分析其TCR β链CDR3的多样性。结果 肝硬化证候LDSD的CDR3独有nt序列数与CDR3独有aa序列数均少于LKYD(P<0.05);LGHD和LKYD的Clonality、Pielous、Shannon.Index和DE50具有显著性差异(P<0.05);3种证型的V和J区基因中多个片段使用频率及V-J基因重组有显著性差异:在LGHD和LDSD中,TRBV21-1、TRBV12-4、TRBV11-1亚型及7对V-J重组有统计学差异(P<0.05);在LGHD和LKYD中,TRBV10-2、TRBV7-6、TRBV5-8亚型及30对V-J重组有统计学差异(P<0.05);在LDSD和LKYD中,TRBJ1-5亚型及18对V-J重组有统计学差异(P<0.05)。结论本研究通过挖掘肝硬化不同证候的免疫学特征,发现肝硬化证候TCR CDR3的多样性具有显著差异且符合由实向虚的证型变化规律,为寻找“病证结合”“辨证论治”的客观依据提供新的支撑,以期发现肝硬化不同证候人群适应性免疫基因重排的表达差异和特异性标记。 展开更多
关键词 肝硬化 中医证候 免疫组库测序 T细胞受体 互补决定区3
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长顺绿壳蛋鸡GRIN1基因3′非翻译区的多态性及其与蛋壳品质的关联性
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作者 罗华伦 王春源 +2 位作者 吴燕 向进 张依裕 《云南农业大学学报(自然科学版)》 CAS CSCD 北大核心 2024年第3期56-63,共8页
【目的】探索长顺绿壳蛋鸡GRIN1基因3′非翻译区(untranslated region,UTR)多态性对蛋壳品质的影响。【方法】选择185只健康蛋鸡,测定其第45周龄产蛋的蛋质量、蛋形指数、蛋壳强度、蛋壳厚度和蛋壳质量5个指标;使用NCBI和PrimerPremier ... 【目的】探索长顺绿壳蛋鸡GRIN1基因3′非翻译区(untranslated region,UTR)多态性对蛋壳品质的影响。【方法】选择185只健康蛋鸡,测定其第45周龄产蛋的蛋质量、蛋形指数、蛋壳强度、蛋壳厚度和蛋壳质量5个指标;使用NCBI和PrimerPremier 3.0设计引物,采用正向测序法筛选GRIN1基因在3′UTR区域的SNP位点。【结果】GRIN1基因的3′UTR检测到4个SNP位点:g.30871C>T、g.31017A>G、g.31158A>C和g.31166G>C,其中仅g.31158A>C和g.31166G>C之间存在强连锁不平衡,且g.30871C>T和g.31017A>G都极显著偏离哈代—温伯格平衡(P<0.01)。g.31017A>G与蛋壳品质的关联分析中,GG基因型的蛋质量显著高于AA基因型(P<0.05)。4个SNP位点共产生5个单倍型(H1、H2、H3、H4、H5)和8个双倍型(H1H1、H1H2、H1H3、H2H2、H2H3、H2H4、H3H5、H4H4),双倍型H3H5的蛋质量显著高于双倍型H2H2和H2H3(P<0.05),双倍型H3H5的蛋壳质量显著高于双倍型H2H2(P<0.05),其他双倍型指标间的差异未达到显著水平(P>0.05)。【结论】长顺绿壳蛋鸡GRIN1基因与蛋壳品质存在显著关联;g.31017A>G对蛋壳品质有显著影响,能够作为改善蛋壳品质的分子标记位点参考。 展开更多
关键词 长顺绿壳蛋鸡 GRIN1基因 3′非翻译区 SNP位点 蛋壳品质
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南极地区BDS-3新频率性能评估
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作者 汤门生 徐儒林 +1 位作者 李学才 谢黎明 《测绘与空间地理信息》 2024年第4期110-112,共3页
针对BDS-3新频率B1C和B2a在南极地区定位性能评估问题,本文基于南极地区CAS1站连续7 d数据,分别进行了BDS-3新频率伪距与精密单点定位实验。实验结果表明,南极地区BDS-3新频率单点定位性能较优,B1C频率和B2a频率伪距单点定位精度可以达... 针对BDS-3新频率B1C和B2a在南极地区定位性能评估问题,本文基于南极地区CAS1站连续7 d数据,分别进行了BDS-3新频率伪距与精密单点定位实验。实验结果表明,南极地区BDS-3新频率单点定位性能较优,B1C频率和B2a频率伪距单点定位精度可以达到米级,B1C/B2a静态精密单点定位精度可以达到厘米级,仿动态精密单点定位水平精度在厘米级,高程精度在分米级,可为今后研究BDS-3在南极地区的定位性能提供一定的参考。 展开更多
关键词 南极地区 BDS-3 新频率 定位性能
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0-3岁婴幼儿阅读发展问卷调查研究中的方法问题——基于中国知识网库文献的内容分析
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作者 钱高垠 潘俊林 《图书馆研究与工作》 2024年第1期37-45,63,共10页
问卷调查是国内0—3岁婴幼儿阅读发展的培育和支持研究中最常采用的方式。文章采用内容分析的方法,对中国知网(CNKI)数据库中的10篇已发表的论文在理论基础、设计、分析和解释等方面进行了分析,旨在总结问卷调查近20年来在促进0—3岁婴... 问卷调查是国内0—3岁婴幼儿阅读发展的培育和支持研究中最常采用的方式。文章采用内容分析的方法,对中国知网(CNKI)数据库中的10篇已发表的论文在理论基础、设计、分析和解释等方面进行了分析,旨在总结问卷调查近20年来在促进0—3岁婴幼儿阅读发展的研究成果,并找出这些研究项目的潜在问题:研究者很少使用理论框架来证明研究问题的合理性;大多数自制设计的调查工具没有建立其有效性和可靠性;没有遵循严格的数据收集程序,并且在分析中没有对结果进行交叉验证。笔者还注意到,目前大部分研究重点都集中在发达的城市地区,如何运用知识基金的理论关注农村,特别是欠发达地区0—3岁婴幼儿的阅读发展,具有迫切的现实意义。 展开更多
关键词 0—3岁婴幼儿 阅读推广 问卷调查 欠发达地区 知识基金
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Polymorphism Analysis of the 3′ Flank Region of Equine IGF-Ⅰ Gene by PCR-SSCP 被引量:5
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作者 孟祥伟 额尔登其木格 +2 位作者 红海 李蓓 芒来 《Agricultural Science & Technology》 CAS 2008年第6期39-42,共4页
[Objective] To explore the polymorphism of the 3′ flank region of equine IGF-Ⅰ gene. [Method] The 3′ flank region sequences of IGF-Ⅰ gene were amplified from genomic DNA of 270 horses, which included 4 types of Mo... [Objective] To explore the polymorphism of the 3′ flank region of equine IGF-Ⅰ gene. [Method] The 3′ flank region sequences of IGF-Ⅰ gene were amplified from genomic DNA of 270 horses, which included 4 types of Mongolian horse, Sanhe horse and Thoroughbred, and then analyzed by PCR-SSCP. [Result] Three genotypes (AA, BB and AB) were detected by PCR-SSCP and the distribution of genotypes of all research objects except Xinihe horse and Baerhu horse were in line with the "Hardy-Weinberg Law". [Conclusion] There was a polymorphic locus in the 3′ flank region of IGF-Ⅰ gene, which might affect the equine growth and development mechanism. The study is of important theoretical and practical significance to improve the performance and to develop equine industry. 展开更多
关键词 IGF-Ⅰ 3′ FLANK region PCR-SSCP
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A study on 3-D velocity structure of crust and upper mantle in Sichuan -Yunnan region, China 被引量:6
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作者 王椿镛 Mooney W.D +3 位作者 王溪莉 吴建平 楼海 王飞 《Acta Seismologica Sinica(English Edition)》 CSCD 2002年第1期1-17,共17页
Based on the first arrival P and S data of 4 625 regional earthquakes recorded at 174 stations dispersed in the Yunnan and Sichuan Provinces, the 3-D velocity structure of crust and upper mantle in the region is deter... Based on the first arrival P and S data of 4 625 regional earthquakes recorded at 174 stations dispersed in the Yunnan and Sichuan Provinces, the 3-D velocity structure of crust and upper mantle in the region is determined, incorporating with previous deep geophysical data. In the upper crust, a positive anomaly velocity zone exists in the Sichuan basin, whereas a negative anomaly velocity zone exists in the western Sichuan plateau. The boundary between the positive and negative anomaly zones is the Longmenshan fault zone. The images of lower crust and upper mantle in the Longmenshan fault, Xianshuihe fault, Honghe fault and others show the characteristic of tectonic boundary, indicating that the faults likely penetrate the Moho discontinuity. The negative velocity anomalies at the depth of 50 km in the Tengchong volcanic area and the Panxi tectonic zone appear to be associated with the temperature and composition variations in the upper mantle. The overall features of the crustal and the upper mantle structures in the SichuanYunnan region are the lower average velocity in both crust and uppermost mantle, the large crustal thickness variations, and the existence of high conductivity layer in the crust or/and upper mantle, and higher geothermal value. All these features are closely related to the collision between the India and the Asia plates. The crustal velocity in the SichuanYunnan rhombic block generally shows normal value or positive anomaly, while the negative anomaly exists in the area along the large strike-slip faults as the block boundary. It is conducive to the crustal block side-pressing out along the faults. In the major seismic zones, the seismicity is relative to the negative anomaly velocity. Most strong earthquakes occurred in the upper-mid crust with positive anomaly or normal velocity, where the negative anomaly zone generally exists below. 展开更多
关键词 regional earthquake Moho discontinuity 3-D velocity structure network method plate collision SEISMICITY
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In vitro effect of amoxicillin and clarithromycin on the 3' region of cagA gene in Helicobacter pylori isolates 被引量:5
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作者 Javier Andrés Bustamante-Rengifo Andrés Januer Matta +1 位作者 Alvaro Pazos Luis Eduardo Bravo 《World Journal of Gastroenterology》 SCIE CAS 2013年第36期6044-6054,共11页
AIM:To evaluate the in vitro effect of amoxicillin and clarithromycin on the cag pathogenicity island(cag PAI).METHODS:One hundred and forty-nine clinical isolates of Helicobacter pylori(H.pylori)cultured from gastric... AIM:To evaluate the in vitro effect of amoxicillin and clarithromycin on the cag pathogenicity island(cag PAI).METHODS:One hundred and forty-nine clinical isolates of Helicobacter pylori(H.pylori)cultured from gastric biopsies from 206 Colombian patients with dyspeptic symptoms from a high-risk area for gastric cancer were included as study material.Antimicrobial susceptibility was determined by the agar dilution method.Resistant isolates at baseline and in amoxicillin and clarithromycin serial dilutions were subjected to genotyping(cagA,vacA alleles s and m),Glu-Pro-Ile-Tyr-Ala(EPIYA)polymerase chain reaction and random amplified polymorphic DNA(RAPD).Images of the RAPD amplicons were analyzed by Gel-Pro Analyzer 4.5program.Cluster analyses was done using SPSS 15.0statistical package,where each of the fingerprint bands were denoted as variables.Dendrograms were designed by following Ward’s clustering method and the estimation of distances between each pair of H.pylori isolates was calculated with the squared Euclidean distance.RESULTS:Resistance rates were 4%for amoxicillin and 2.7%for clarithromycin with 2%double resistances.Genotyping evidenced a high prevalence of the genotype cagA-positive/vacA s1m1.The 3’region of cagA gene was successfully amplified in 92.3%(12/13)of the baseline resistant isolates and in 60%(36/60)of the resistant isolates growing in antibiotic dilutions.Upon observing the distribution of the number of EPIYA repetitions in each dilution with respect to baseline isolates,it was found that in 61.5%(8/13)of the baseline isolates,a change in the number of EPIYA repetitions lowered antibiotic pressure.The gain and loss of EPIYA motifs resulted in a diversity of H.pylori subclones after bacterial adjustment to changing conditions product of antibiotic pressure.RAPD PCR evidenced the close clonal relationship between baseline isolates and isolates growing in antibiotic dilutions.CONCLUSION:Antibiotic pressure does not induce loss of the cag pathogenicity island,but it can leadin most cases-to genetic rearrangements within the3’region cagA of the founding bacteria that can affect the level of tyrosine phosphorylation impacting on its cellular effects and lead to divergence of cagA-positive subclones. 展开更多
关键词 HELICOBACTER PYLORI Antimicrobial susceptibility cag pathogenicity island CAGA 3region Random amplified POLYMORPHIC DNA-polymerase chain reaction
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Joint Inversion of the 3D P Wave Velocity Structure of the Crust and Upper Mantle under the Southeastern Margin of the Tibetan Plateau Using Regional Earthquake and Teleseismic Data 被引量:12
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作者 LI Dahu LIAO Hua +4 位作者 DING Zhifeng ZHAN Yan WU Pingping XU Xiaoming ZHENG Chen 《Acta Geologica Sinica(English Edition)》 CAS CSCD 2018年第1期16-33,共18页
The special seismic tectonic environment and frequent seismicity in the southeastern margin of the Qinghai-Tibet Plateau show that this area is an ideal location to study the present tectonic movement and background o... The special seismic tectonic environment and frequent seismicity in the southeastern margin of the Qinghai-Tibet Plateau show that this area is an ideal location to study the present tectonic movement and background of strong earthquakes in China's Mainland and to predict future strong earthquake risk zones. Studies of the structural environment and physical characteristics of the deep structure in this area are helpful to explore deep dynamic effects and deformation field characteristics, to strengthen our understanding of the roles of anisotropy and tectonic deformation and to study the deep tectonic background of the seismic origin of the block's interior. In this paper, the three-dimensional (3D) P-wave velocity structure of the crust and upper mantle under the southeastern margin of the Qinghai-Tibet Plateau is obtained via observational data from 224 permanent seismic stations in the regional digital seismic network of Yunnan and Sichuan Provinces and from 356 mobile China seismic arrays in the southern section of the north-south seismic belt using a joint inversion method of the regional earthquake and teleseismic data. The results indicate that the spatial distribution of the P-wave velocity anomalies in the shallow upper crust is closely related to the surface geological structure, terrain and lithology. Baoxing and Kangding, with their basic volcanic rocks and volcanic clastic rocks, present obvious high-velocity anomalies. The Chengdu Basin shows low-velocity anomalies associated with the Quaternary sediments. The Xichang Mesozoic Basin and the Butuo Basin are characterised by low- velocity anomalies related to very thick sedimentary layers. The upper and middle crust beneath the Chuan-Dian and Songpan-Ganzi Blocks has apparent lateral heterogeneities, including low-velocity zones of different sizes. There is a large range of low-velocity layers in the Songpan-Ganzi Block and the sub-block northwest of Sichuan Province, showing that the middle and lower crust is relatively weak. The Sichuan Basin, which is located in the western margin of the Yangtze platform, shows high-velocity characteristics. The results also reveal that there are continuous low-velocity layer distributions in the middle and lower crust of the Daliangshan Block and that the distribution direction of the low-velocity anomaly is nearly SN, which is consistent with the trend of the Daliangshan fault. The existence of the low-velocity layer in the crust also provides a deep source for the deep dynamic deformation and seismic activity of the Daliangshan Block and its boundary faults. The results of the 3D P-wave velocity structure show that an anomalous distribution of high-density, strong-magnetic and high-wave velocity exists inside the crust in the Panxi region. This is likely related to late Paleozoic mantle plume activity that led to a large number of mafic and ultra-mafic intrusions into the crust. In the crustal doming process, the massive intrusion of mantle-derived material enhanced the mechanical strength of the crustal medium. The P-wave velocity structure also revealed that the upper mantle contains a low-velocity layer at a depth of 80-120 km in the Panxi region. The existence of deep faults in the Panxi region, which provide conditions for transporting mantle thermal material into the crust, is the deep tectonic background for the area's strong earthquake activity. 展开更多
关键词 3D P-wave velocity structure China seismic array detection Panxi region Chuan-DianBlock Daliangshan Block southeastern margin of Qinghai-Tibet Plateau
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Efects of Cropland Cover Changes on Regional Climate over Western China Based on Simulations with RegCM3 被引量:2
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作者 SHI Xue-Li HE Hui-Juan REN Hong-Chang 《Advances in Climate Change Research》 SCIE 2013年第4期250-259,共10页
The impacts of land cover changes on regional climate with RegCM3. Sensitivity experiments were conducted by in Shaan-Gan-Ning (SGN) in western China were simulated replacing crop grids with different new land cover... The impacts of land cover changes on regional climate with RegCM3. Sensitivity experiments were conducted by in Shaan-Gan-Ning (SGN) in western China were simulated replacing crop grids with different new land cover types in the key area of SGN, where the returning cropland to tree/grass project has been carried out since 1999. The modified new land cover types include desert, forest, shrub and grass. They represent degraded, improved, and maintained vegetation cover with natural canopy in the key area. Results from three individual case studies show that the land cover change causes changes in temperature and terrestrial water variables especially within the key area, while changes in precipitation are found for a larger area. The strongest changes appear where the cropland is degraded to bare soil, leading to increasing temperature and decreases in rainfall, evaporation and soil water. Opposite changes occur when cropland changed into forests, especially with strong increases in soil water. When cropland changed to grass and shrub land, the climatic changes are closer to those with forest cover. This shows the importance of improving and maintaining the vegetation in SGN for the ecosystem and regional climate. 展开更多
关键词 land cover change REGCM3 regional climate
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