The anti-cancer effects of betulinic acid （BA） on Jurkat cells and its in vitro mechanism were examined by using MTT assay. Apoptosis was detected by using Hoechst33258 staining and annexin-Ⅴ/PI double-labeled cyto...The anti-cancer effects of betulinic acid （BA） on Jurkat cells and its in vitro mechanism were examined by using MTT assay. Apoptosis was detected by using Hoechst33258 staining and annexin-Ⅴ/PI double-labeled cytometry. The effects of betulinic acid on the cell cycle of Jurkat cells were studied by propidium iodide method. RT-PCR and Western blotting were used to analyze the changes of cyclin D3, bcl-xl mRNA and protein levels in Jurkat cells after treatment with betulinic acid. Our results showed the proliferation of Jurkat cells was decreased in betulinic acid-treated group with a 24-h IC50 value being 70.00 μmol/L. Betulinic acid induced apoptosis of Jurkat cells in a time-and dose-dependent manner. The number of Jurkat cells treated with betulinic acid showed an increase in G0/G1 phase and decrease in S phase. After treatment with 0, 20, 60, 100 μmol/L betulinic acid for 24 h, the number of Jurkat cells was increased from （31.00±1.25）% to （58.84±0.32）% in G0/G1 phase, whereas it was decreased from （61.45±1.04）% to （35.82±1.95）% in S phase. PBMCs were less sensitive to the cytotoxicity of betulinic acid than Jurkat cells. The expressions of cyclin D3, bcl-xl mRNA and protein were decreased sharply in Jurkat cells treated with betulinic acid. It is concluded that betulinic acid is able to inhibit the proliferation of Jurkat cells by regulating the cell cycle, arrest cells at G0/G1 phase and induce the cell apoptosis. The anti-tumor effects of betulinic acid are related to the down-regulated expression of cyclin D3 and bcl-xl.展开更多
The effects of betulinic acid (BA), a pentacyclic lupane-type triterpene, on the cell viability, cell cycle and apoptosis in human leukemia K562 cells were investigated. The effects of BA on the growth of K562 cells w...The effects of betulinic acid (BA), a pentacyclic lupane-type triterpene, on the cell viability, cell cycle and apoptosis in human leukemia K562 cells were investigated. The effects of BA on the growth of K562 cells were studied by MTT assay. Apoptosis was assayed through Annexin V/propidium iodide (PI) double-labeled cytometry. The effects of BA on the cell cycle of K562 cells were studied by a PI method. The expression of Bax and capase-3 was detected by using Western blot. The results showed that BA was cytotoxic to K562 cells with an IC50 of 21.26 μg/mL at 24 h. After treating K562 cells with 10 μg/mL BA for 72 h, the number of cells was reduced by 58%. BA induced apoptosis of K562 cells in a time-and dose-dependent manner. The proportion of cells in G0/G1 and G2/M phases was decreased and that in S phase was increased after K562 cells were treated with BA for 24 h. BA treatment also increased the expression of the pro-apoptotic proteins Bax and caspase-3. It suggested that BA could inhibit the proliferation of K562 cells through the induction of cell cycle arrest and apoptosis. The antitumor effects of BA were related with up-regulation of the expression of Bax and caspase-3 proteins. BA may qualify for the development of new therapies for leukemia.展开更多
钟萼木(Bretschneidera sinensis Hemsl.)又名伯乐树,系钟萼木科(Bretschnei-deraceae)钟萼木属的稀有保护植物。该科仅此1属1种,为我国特有山。分布于云南、广东、广西、江西、浙江、湖南、贵州和四川,生于海拔500—1500 m 的山地林中...钟萼木(Bretschneidera sinensis Hemsl.)又名伯乐树,系钟萼木科(Bretschnei-deraceae)钟萼木属的稀有保护植物。该科仅此1属1种,为我国特有山。分布于云南、广东、广西、江西、浙江、湖南、贵州和四川,生于海拔500—1500 m 的山地林中。树皮有祛风活血作用。钟萼木的化学成分尚未见报道。为研究其活性物质。展开更多
our new saponins were isolated from the leaves of Oplopanax elatus Nakai.The structures of the four saponins , tentatively named compounds A(1) , B(2) , C(3) and D (4) were elucidated to be 28-O-a-L-rhamnopyranosyl (1...our new saponins were isolated from the leaves of Oplopanax elatus Nakai.The structures of the four saponins , tentatively named compounds A(1) , B(2) , C(3) and D (4) were elucidated to be 28-O-a-L-rhamnopyranosyl (1-4)-β-D-glu-copyranosyl ( 1-6 )-β-D-glucopyranosyl esters of betulinic acid 3-O-β-D-glucopyra-noside (1) . oleanolic acid 3-O-β-D-glucopyranoside (2) , 3a, 23-dihydroxyolean-12-ene-28-Oic acid ( = 3-epi-hederagenin ) (3) , 3β-hydroxyolean-12-ene-23-al-28-oic acid (=gypsogenin) (4) , respetively.展开更多
gain, four new saponins were isolated from tlie leaves of Oplopanax elatusHaka,. The structures of the four saponins ,tentatively named cirensenoside E(1),F(2), G(3) and H(4) , were elucidated to be 28-O-α-L-rlianino...gain, four new saponins were isolated from tlie leaves of Oplopanax elatusHaka,. The structures of the four saponins ,tentatively named cirensenoside E(1),F(2), G(3) and H(4) , were elucidated to be 28-O-α-L-rlianinopyranosyl(1→4)-β-D-glucopyranosyl(1→6)-β-D-g-Dglucopyranosyl esters of 3epi-betulinic acid 3-O-β-D-glucopyranoside (1), 3β-hydroxylup-20 (29)-en-23.28-dioic acid (2),3β, 23-di-hydroxylup-20 (29 )-en-28-oic acid (3), 3α, 23-dihydroxylup-20 (29)-en-28-oic acidt4), respectively.展开更多
目的建立多组分定量联合化学计量学及熵权TOPSIS分析对不同产地所得青钱柳Cyclocarya paliurus质量进行评价的方法,为青钱柳药材的品质评价和质量控制提供参考。方法取不同产地所得18批青钱柳为检测样品,以熊果酸为内参物,采用一测多评(...目的建立多组分定量联合化学计量学及熵权TOPSIS分析对不同产地所得青钱柳Cyclocarya paliurus质量进行评价的方法,为青钱柳药材的品质评价和质量控制提供参考。方法取不同产地所得18批青钱柳为检测样品,以熊果酸为内参物,采用一测多评(quantitative analysis of multi-components by single-marker,QAMS)法同时测定青钱柳中14个成分的含量,运用化学计量学和熵权TOPSIS分析法对不同产地青钱柳进行比较分析和综合评价。结果含量测定方法学考察结果符合中国药典规定要求。以熊果酸为内参物的相对校正因子稳定性、耐用性良好(RSD均小于2.0%),外标法实测值与QAMS法计算结果没有显著差异。化学计量学方法显示18批青钱柳可聚为3类,呈现一定的区域差异;槲皮素-3-O-葡萄糖醛酸苷、金丝桃苷、熊果酸、阿福豆苷和异槲皮苷是影响青钱柳产品质量的主要成分;EW-TOPSIS法分析结果显示贵州和四川地区所得青钱柳质量最优,其次为湖南、江西和安徽。结论所建方法快速灵敏、准确可靠,可用于青钱柳内在质量的综合质量评价。展开更多
基金supported by a grant from the National Natural Sciences Foundation of China (No. 30500686).
文摘The anti-cancer effects of betulinic acid （BA） on Jurkat cells and its in vitro mechanism were examined by using MTT assay. Apoptosis was detected by using Hoechst33258 staining and annexin-Ⅴ/PI double-labeled cytometry. The effects of betulinic acid on the cell cycle of Jurkat cells were studied by propidium iodide method. RT-PCR and Western blotting were used to analyze the changes of cyclin D3, bcl-xl mRNA and protein levels in Jurkat cells after treatment with betulinic acid. Our results showed the proliferation of Jurkat cells was decreased in betulinic acid-treated group with a 24-h IC50 value being 70.00 μmol/L. Betulinic acid induced apoptosis of Jurkat cells in a time-and dose-dependent manner. The number of Jurkat cells treated with betulinic acid showed an increase in G0/G1 phase and decrease in S phase. After treatment with 0, 20, 60, 100 μmol/L betulinic acid for 24 h, the number of Jurkat cells was increased from （31.00±1.25）% to （58.84±0.32）% in G0/G1 phase, whereas it was decreased from （61.45±1.04）% to （35.82±1.95）% in S phase. PBMCs were less sensitive to the cytotoxicity of betulinic acid than Jurkat cells. The expressions of cyclin D3, bcl-xl mRNA and protein were decreased sharply in Jurkat cells treated with betulinic acid. It is concluded that betulinic acid is able to inhibit the proliferation of Jurkat cells by regulating the cell cycle, arrest cells at G0/G1 phase and induce the cell apoptosis. The anti-tumor effects of betulinic acid are related to the down-regulated expression of cyclin D3 and bcl-xl.
基金supported by a grant from the National Natural Sciences Foundation of China (No. 30500686)
文摘The effects of betulinic acid (BA), a pentacyclic lupane-type triterpene, on the cell viability, cell cycle and apoptosis in human leukemia K562 cells were investigated. The effects of BA on the growth of K562 cells were studied by MTT assay. Apoptosis was assayed through Annexin V/propidium iodide (PI) double-labeled cytometry. The effects of BA on the cell cycle of K562 cells were studied by a PI method. The expression of Bax and capase-3 was detected by using Western blot. The results showed that BA was cytotoxic to K562 cells with an IC50 of 21.26 μg/mL at 24 h. After treating K562 cells with 10 μg/mL BA for 72 h, the number of cells was reduced by 58%. BA induced apoptosis of K562 cells in a time-and dose-dependent manner. The proportion of cells in G0/G1 and G2/M phases was decreased and that in S phase was increased after K562 cells were treated with BA for 24 h. BA treatment also increased the expression of the pro-apoptotic proteins Bax and caspase-3. It suggested that BA could inhibit the proliferation of K562 cells through the induction of cell cycle arrest and apoptosis. The antitumor effects of BA were related with up-regulation of the expression of Bax and caspase-3 proteins. BA may qualify for the development of new therapies for leukemia.
文摘钟萼木(Bretschneidera sinensis Hemsl.)又名伯乐树,系钟萼木科(Bretschnei-deraceae)钟萼木属的稀有保护植物。该科仅此1属1种,为我国特有山。分布于云南、广东、广西、江西、浙江、湖南、贵州和四川,生于海拔500—1500 m 的山地林中。树皮有祛风活血作用。钟萼木的化学成分尚未见报道。为研究其活性物质。
文摘our new saponins were isolated from the leaves of Oplopanax elatus Nakai.The structures of the four saponins , tentatively named compounds A(1) , B(2) , C(3) and D (4) were elucidated to be 28-O-a-L-rhamnopyranosyl (1-4)-β-D-glu-copyranosyl ( 1-6 )-β-D-glucopyranosyl esters of betulinic acid 3-O-β-D-glucopyra-noside (1) . oleanolic acid 3-O-β-D-glucopyranoside (2) , 3a, 23-dihydroxyolean-12-ene-28-Oic acid ( = 3-epi-hederagenin ) (3) , 3β-hydroxyolean-12-ene-23-al-28-oic acid (=gypsogenin) (4) , respetively.
文摘gain, four new saponins were isolated from tlie leaves of Oplopanax elatusHaka,. The structures of the four saponins ,tentatively named cirensenoside E(1),F(2), G(3) and H(4) , were elucidated to be 28-O-α-L-rlianinopyranosyl(1→4)-β-D-glucopyranosyl(1→6)-β-D-g-Dglucopyranosyl esters of 3epi-betulinic acid 3-O-β-D-glucopyranoside (1), 3β-hydroxylup-20 (29)-en-23.28-dioic acid (2),3β, 23-di-hydroxylup-20 (29 )-en-28-oic acid (3), 3α, 23-dihydroxylup-20 (29)-en-28-oic acidt4), respectively.
文摘目的建立多组分定量联合化学计量学及熵权TOPSIS分析对不同产地所得青钱柳Cyclocarya paliurus质量进行评价的方法,为青钱柳药材的品质评价和质量控制提供参考。方法取不同产地所得18批青钱柳为检测样品,以熊果酸为内参物,采用一测多评(quantitative analysis of multi-components by single-marker,QAMS)法同时测定青钱柳中14个成分的含量,运用化学计量学和熵权TOPSIS分析法对不同产地青钱柳进行比较分析和综合评价。结果含量测定方法学考察结果符合中国药典规定要求。以熊果酸为内参物的相对校正因子稳定性、耐用性良好(RSD均小于2.0%),外标法实测值与QAMS法计算结果没有显著差异。化学计量学方法显示18批青钱柳可聚为3类,呈现一定的区域差异;槲皮素-3-O-葡萄糖醛酸苷、金丝桃苷、熊果酸、阿福豆苷和异槲皮苷是影响青钱柳产品质量的主要成分;EW-TOPSIS法分析结果显示贵州和四川地区所得青钱柳质量最优,其次为湖南、江西和安徽。结论所建方法快速灵敏、准确可靠,可用于青钱柳内在质量的综合质量评价。
